한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

차량 재도색용 청색 페인트의 분석

박성우,손성건,박하선,박성지,정영호,홍성욱,박종신 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

CD34+ 조혈 모세포 이식 2례

김정아,정현식,김원석,윤성수,이홍기,박찬형,박성규,김동욱,이종욱,한치화,민우성,김춘추,김동집 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

Background: In most solid tumors, the CD34 antigen has not been detected, so positive selection of CD34+ cells may reduce tumor cell contamination and the CD34+ cells are capable of reconstituting hematopoiesis. We tried CD34+ cell transplantation in two patients. Method: CD34+ cells from chemotherapy + G-CSF mobilized PBPCs or bone marrow were positively selected with an avidin-biotin immunoadsorption column (CEPRATE SC system). Case 1. One course of chemotherapy using cyclophosphamide(200㎎/㎡) and etoposide (4.2g/㎡), combined with G-CSF(5㎍/㎏) S.C. was used in a relapsed lymphoma patient. This patient responded to the induction chemotherapy. CD34+ cells from harvested bone marrow were selected by the CellPro immunoadsorption column. The total number of mononuclear cells loaded onto the CellPro was 2.4×10^(8)/㎏, with 1.1% CD34+ cells. After column separation, the total number of positively selected cells was 5.16×10^(6)/㎏. The number of CFU-GM was 76.8×10⁴/㎏. This patient was treated with melphalan (140㎎/㎡) and TBI (1200cGy) and the positively selected CD34+ cells were infused. The time to neutrophil recovery greater than 0.5×10^(9)/L was 19 days and the time to platelet recovery greater than 50×10^(9)/L was 21 days. Case 2. Two courses of mobilizing chemotherapy were given 4 weeks apart using taxol(210㎎/m2) and adriamycin(60㎎/m2), combined with G-CSF(5㎍/㎏) S.C. in a breast cancer patients with 7 axillary node metastasis. CD34+ cells from each single leukapheresis product were selected by the CellPro immunoadsorption column. In the first collection, the total number of nucleated cells was 4.4×10^(8)/kg, with 0.42% CD34+ cells. In the second collection, the total number of nucleated cell was 2.8× 10^(8)/㎏ with 0.43% CD34+ cells. After colum separation, the total numbers of collected cells were 4.0×106/kg and 4.8×10^(6)/kg, the total number of CD34+ cells were 1.2×10^(6)/㎏ and 0.82×10^(6)/㎏. Colonogenic assays of positively selected CD34+ cells gave rise to myeloid erythroid, and multilineage colonies, with a median of 190 CFU-GM, 190 BFU-E, and 164 CFU-GEMM per 1×10³ adsorbed cells, respectively. High-dose chemotherapy with cumulative doses of 40mg/㎡ mitoxantrone, 750mg/㎡ thioptepa, and 1000mg/㎡ carboplatin was administered. Positively selected CD34+ cells were rapidly infused 24 hours after the end of high-dose chemotherapy. The time to neutrophil recovery greater than 0.5×10^(9)/L was 16 days and the time to platelet recovery greater than 50×10^(9)/L was 20 days.

오리부산물과 한약재를 이용한 추출액의 영양성분 및 중금속 노출에 대한 피해 완화 효과 : (Ⅱ) 중금속 노출에 대한 오리추출액의 피해 완화 효과 (Ⅱ) Damage Mitigation Effects on Heavy-metals Exposure of Peking-Duck By-Product Extracts

한종현,이우진,조성균,이미정,정미란,전정우,김운영,박성혜 동아시아식생활학회 2003 동아시아식생활학회지 Vol.13 No.4

This study was performed to investigate the effects of peking-duck extracts added with medicinal herbs(DJ) on the intoxication of heavy metals (Hg, Pb, Cd, As) in rats. Sprague-Dawley rats weighing 150±15g, were randomly assigned to 5 groups: basal diet only in normal control group(NCG); basal diet and heavy metals without DJ injection in heavy metal control group(HMC); basal diet, heavy metals and DJ(3㎎/㎖) injection in heavy metal low duck juice group(HMLD), basal diet, heavy metals and DJ(30㎎/㎖) injection in heavy metal middle duck juice group(HMMlD); basal diet, heavy metal and DJ(300㎎/㎖) injection in heavy metal high duck juice group(HMHD). Hg and As was injected by 50ppm and Cd and Pb by 25ppm for 17days. Also DJ oral feeding was conducted for 28days. The result of this study were as follows: Food intake and body weight gain in heavy metal administered groups were lower than those of NCG. Liver, kidney and testis weights were not significantly different among 5 groups. GOT, GPT and BUN activities were significantly reduced in DJ treated groups as compared to HMC. DJ showed the suppressing effect on the accumulation of Hg, Pb and Cd in serum, liver and kidney. Fecal Hg and Cd excretions increased with DJ feeding. The results suggested that DJ may have some protective effects on Hg, Cd and Pb intoxication by reducing the accumulation m tissues and increasing excretion. This study also showed the effective way of using duck-extract and its application to the oriental medicine.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

유관속 식물 분류의 강과 생태형에 따른 잎 전후면의 기공 분포

鄭宇珪,成敏雄,尹福任 慶尙大學校 1989 論文集 Vol.28 No.2

관속실물 118종이 잎 전후면의 기공분포와 생태학적 식물형과 계통 분류와의 연관성을 찾기 위하여 1987년부터 1988년까지 2년에 걸쳐 경상대학교 가좌 캠퍼스 내의 식물을 채집하여 잎 전후면의 기공분포를 조사한 결과는 다음과 같았다. 1. 고사리류는 기공이 잎의 후면에만 분포하였다. 나자식물, 외떡잎식물, 쌍떡잎식물에서는 잎의 전후면에 기공이 분포하였으며, 기공 분포 비율은 잎 후면에서 각각 66%, 66%및 85%로 높게 나타났다. 2. 나자식물 8종 중에서 소철, 은행나무, 주목 및 전나무 4종은 잎 후면에만 기공이 분포하였고, 평균적으로 전면 43, 후면 65였다. 3. 외떡잎식물 34종의 경우 잎 전면에만 기공이 분포하는 식물은 가래와 죽순대 2종이었고, 후면에만 기공이 분포하는 식물은 백합, 청미래덩굴, 보춘화 및 닭의 난초 4종이었다. 나머지 31종은 잎 전후면에 기공이 분포하였고, 평균 전면 58, 후면 92였다. 4. 쌍떡잎 식물 70여종 중에서 38종은 기공이 잎의 후면에만 존재하였고, 나머지 32종은 전후면에 모두 분포하고 있었고, 평균 전면 27 후면 175이었다. 5. 초본과 목본의 비교에서 기공수는 잎의 후면에서는 목본이 158(85%)개로 초본 120(68%)개 보다 많았고, 잎 전면에서는 목본이 14(15%)개로 초본 58(30%)개 보다 적었다. 상록수와 낙엽수 비교해서 잎의 후면에서는 낙엽수가 201(99%)개로 상록수 126(75%)개 보다 많았고, 전면에서는 상록수가 12(16%)개로 낙엽수 3(1%)개 보다 많았다. 6. 다육식물과 비다육식물 비교해서 기공 분포는 다육 식물의 경우 ㎟단위 면적당 평균 기공 수가 잎 전면에서 33개, 잎 후면에서 34(50%)개, 길이는 각각41㎛로 잎 전후면이 유사하게 나타났다. 비다육식물은 ㎟단위 면적당 평균 기공 수가 잎 전면에서 36(19%)개, 잎 후면에서 149(81%)개로 잎 후면이 높게 나타났다. 7. 잎 전후면 1㎟당 분포하는 평균 기공 수를 합친 기공 수는 속새강 114개, 고사리류 104개, 나자식물 108개, 외떡잎식물 140개, 쌍떡잎식물 203개로 하등에서 고등으로 갈수록 기공 수가 높게 나타났다. A total of 118 species of vascular plants collected at Kajoa Campus, Gyeongsang National University from 1987 to 1988 was observed to determine their systematic relations and ecological plant form by analyzing the distribution of the stomata on both sides of the leaves. 1. The stomata were distributed only on lower surface of leaves in Filicinae, but they were found on both upper and lower surface of leaves in Gymnospermae, Monocotyledoneae and Dicotyledoneae. The stomatal distribution on lower surface of leaves apperared high 66% in Gymnospermae, 66% in Monocotyledonae and 66% in Dicotyledonae of the total number of stomata on both upper and lower surfaces of leaves. 2. The stomata were distributed only on lower surface of leaves in Cycas revoluta, Ginkgo biloba, Taxus cuspidata and Abies holophylla, but they were found on upper surface of leaves in pinus koraiensis, among eight species of Gymnospermae. The average number was 43 on upper surface and 65 on lower surface of leaves. 3. The stomata were distributed only on upper surface of leaves in Potamogeton distinctus and Phyllostachys pubescens, they were found on lower surface of leaves in Lilium longiflorunn, Smilax china, Cymbidium goeringii and Epipactis thunbergii, and they were distributed on both upper and lower surfaces of leaves in twenty- eight of the other thirty-five species of Monocotyledoneae. The average number was 58 on upper surface and 92 on lower surface of leaves. 4. The stomata were distributed only on lower surface of leaves in thirty-eight species in total of seventy species of Dicotyledoneae and they were found upper and lower surfaces of leaves in thirty-two species of all seventy species of Dicotyledoneae. The average number was 27 on upper surface and 175 on lower surface of leaves. 5. The stomatal number, or 158(85%) on lower surface of leaves in wood plant was higher in distribution than that or 120(68%) on lower surface of leaves in the herbaceous. The stomatal number, or 14(15%), on upper surface of leaves in wood plant was smaller in distribution than stomatal number, or 58(30%) on upper the herbaceous. The stomatal number, or 201(99%) on lower surfaces of leaves was more densely distributed in deciduous trees than the stomatal number, or 126(75%) on lower surface of leaves in evergreen trees, The stomatal number, or 12(16%) on upper surface was more densely distributed in evergreen trees than the stomatal number, or 3(1%), on the upper surface of leaves in deciduous trees. 6. The succulent plant was equal in number and size of stomata on both upper and lower surflaces of leaves. In other words it had 33-34 Stomata (50%) respecitively and 41μm length in size of stomata. But the non-succulent plant hard 36 stomata(19%) on the upper surface of leaves and 149(81%) stomata on the lower surface of leaves. The number and size of stomata in the succulent plant appeared similar in comparison with those in the non-succulent plant. 7. The total number of stoamta per ㎟ on both upper and lower surfaces was 114 in Equisetineae, 104 in Fern, 108 in Gymnospermal, 140 in Monocotyledoneae and 203 in Dicotyledoneae. The stomatal number proved to be more and more increasely in sequence of Equisetineae, Filicinae, Gymospermae, Momocotyledoneae, and Dicotyledoneae.

새로운 구조의 챠지펌프 방식를 사용한 직류-직류 변환기설계

한정우,손상희 청주대학교 산업과학연구소 2014 産業科學硏究 Vol.31 No.2

In this paper, output voltage conversion type of DC to DC converter using charge pump method is newly proposed and designed. In conventional cross-coupled charge pump, simulation results show that output voltage is 5.93V and efficiency is 99% and numbers of capacitor are 3 in case of step-up factor, 2. But simulation results using proposed charge pump in same condition show that output voltage is 5.96V and efficiency is 99% and numbers of capacitor are 2. From the simulation results, we know that output voltage and efficiency is similar between cross-coupled charge pump and proposed charge pump. But reducing capacitor makes chip size small and decrease cost down. In the proposed circuit, it is possible to remove the body effect secondary effective MOS by adding a body control system. It is possible to prevent that it is possible to prevent the shift in the threshold voltage of the MOS by the body controls, a result, the efficiency of the overall circuit is reduced. Also output voltage conversion is accomplished by varying the resistance ratio of the switch. Circuit simulations are executed with the design rule of magnachip 5V/30V 0.7um power-CMOS(Complementary Metal-Oxide Semiconductor) process and Cadence Spectre simulator.