서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

악관절 과두강직 : 편측성 과두강직을 동반한 안모비대칭 치료 증례 UNILATERAL POSTTRAUMATIC CONDYLAR PSEUDOANKYLOSIS

홍성규,홍성준 대한치과교정학회 1993 대한치과교정학회지 Vol.23 No.3

The ankylosis of temporomandibular joint occured by several causes directly developes TM joint disfunction, In cases with prolonged condylar ankylosis, especially at growing age the condylar ankylosis evokes malfunction of growth center area, and then developes the abnormal facial morphology and malocclusion. Therefore it must be cured. Almost authors have agreed to the necessity of surgical correction of the TMJ anlylosis. but they did not decide the one surgical method to get the best results. The Tx. method suggested by many authors are the using interposition after resection of condyle to remain a lever of 3rd class in Mn. kinetics, the autogenous condylar graft and the alloplastic condylar graft. Some authors have got the satisfied results only with the condylectomy of the involved TMJ. This study also operated only the detachment of fibrous adhesion on ankylosed condylar side and then established occlusion in the case with the unilateral TMJ ankylosis and fibrous joint adhesion and facial asymmetry evoked after the fracture of condylar head at early age. This study got a improved mouth opening and a stable postsurgical result after 1 year. Also, this study reviewed many author's study about the chanracteristics, etiology, diagnosis and Tx. method for the ankylosis of TMJ.

간질발작후 MRI 영상에서 뇌 종창을 보인 3예 보고

홍현숙,김대호,이혜경,박재성,박성진,조준희,차장규,백상현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

We report seizure induced brain swelling in three patients. MRI revealed a cortical, subcortical(3/3), and basal ganglia(2/3) high signal intensity on T2- weighted and FLAIR images. The lesion shows low signal on T1-weighted image, and enhanced after contrast administration. Follow up study after 27 days in one case revealed complete resolution of the lesion. The reversible MRI findings may be the results of a local brain swelling, breakdown of blood-brain permeability, and a defect of cerebral autoregulation during seizure activity. The transient nature of such findings have to be considered in the differential diagnosis such as reversible ischemia, migraine, inflammation and infection.

구순 구개열 신생아에 적용한 Obturator의 효과에 관한 증례보고

홍성준,이긍호,최영철 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.2

구순 구개열 신생아는 출생시부터 수유의 어려움을 비롯하여 중이염, 언어장애, 부정교합, 부적절한 악안면 성장과 이로 인한 정신적 손상등에 직면하게 된다. 이런 복합적인 문제를 갖고 있는 신생아의 전반적인 치료를 위해서는 여러 관련분야 전문가들의 포괄적인 관리가 필요하다. 특히 수유의 어려움은 유아의 영양상태를 좌우할 수 있는 중요한 문제이므로 가장 먼저 관리하여야 할 부분이다. Obturator의 사용은 수유보조의 역할 뿐만 아니라 구강과 비강을 구분하여 중이염의 발생빈도를 감소시키고, 구개판의 성장을 촉진시키며, 정상적인 구강 안면조직의 발달을 유도할 수 있다. 소아치과 의사는 구순 구개열 신생아의 정상적인 성장, 발육을 위해 출생 직후 부모에게 적절한 수유방법의 조언과 함께 obturator를 장착해 주고, 다른 team과 긴밀한 협진을 통해 유치열기, 혼합치열기까지 지속적으로 치과적 관리를 제공하여야 한다. It has been reported that There is a relatively high incidence of congenital cleft lip and palate among congenital anomalies. Children with the cleft lip and palate confront various problems ranging from feeding difficulties as infants to frequebt ear infections, compromised speech, aberrant dentofacial development, malocclusion and esthetic impairment with its unfavorable effects on the psychosocial adaptation of the individual. Management of these patients is a process that starts in infancy and continues on into adulthood. Through a team approach, professionals from various fields convene to assess the needs of the child and to assist the parents in dealing with the situation. A pediatric dentist, one of the team, must advise the parents the way of feeding or make an obturator for normal feeding and weight gain of infants with cleft lip and/or palate. An obturator can aid nursing, stimulate orofacial development, help develop the palatal shelves, prevent tongue distortions, prevent nasal septum irritation, decrease chance of ear infections, expand collaped maxillary segments, constrict the expanded anterior part of the maxilla, reposition the premaxilla, and help the parents psychologically. Three cases of infants with cleft lip and palate were managed with the obturator at the Dept. of Pediatric Dentistry, Kyung Hee University. In all cases, infants showed normal feeding and weight gaining after the insertion of the obturator.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

표피성장인자, 염기성 섬유모세포 성장인자 및 각질세포 성장인자가 LNCaP 전립선암 세포주의 PSA 분비와 PSA mRNA 발현에 미치는 영향

이홍우,김준철,서성일,황태곤 大韓泌尿器科學會 2001 Korean Journal of Urology Vol.42 No.8

TNF계 CD137L 및 RANKL의 파골세포와 T 세포에 대한 활성조절

홍성준,박재홍,이현우,이긍호 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.4

본 연구는 TNFR family인 CD137 및 RANK. 파골세포의 CD137L와 T 세포의 RANKL 간의 역신호에 의한 이들 세포의 역할을 알아보고자 하였다. 이에 RANKL 및 CD137L 자극으로 유도되는 역신호 전달에 의한 T 세포 활성과 파골세포분화에 미치는 영향을 규명하고자 웅성 생쥐의 골수세포와 T 세포를 공동배양하여 다음과 같은 결과를 얻었다. 1. 생쥐 단핵세포주 및 골수유도 단핵전구세포에서 CD137L이 발현되며, CD137L 단클론 항체로 자극을 주었을 경우 파 골세포 표지단백절인 TRAP 양성 파골세포의 형성이 억제되었다. 2. 활성화된 CD4^(+) 및 CD8^(+) T 세포에서 RANKL을 발현하였으며 RANKL의 유사 수용체인 OPG 재조합 단백질을 처리하여 CD4^(+) 및 CD8^(+) T 세포의 세포증식이 억제되었다. 이 연구의 결과는 CD137자극에 의한T세포활성 및 RANK 자극에 의한파골세포분화및 활성이 각각수용체에 결합하는 라이겐드의 역신호에 의해 억제되었는데, 이는 파골세포와 T 세포의 과도한 활성을 제어하는 생체의 항상성조절에 관여하는 기전으로 생각된다. Resorption of alveolar bone in periodontitis is due to excessive differentiation and activation of osteoclasts. Bacterial antigens causing periodontitis activates CD4 T cells, which leads to expressing RANK ligand (RANKL) on CD4 T cells. RANKL binds RANK on preosteoclasts or osteoclasts, and enhances the differentiation preosteoclasts into osteoclasts and the activation of mature osteoclasts. CD137, one of TNF receptor (TNFR) family, expressed on activated T cells binds with CD137 ligand (CD137L) on antigen presenting cells. Cross-linking of CD137 by CD137L acts as T cell co-stimulatory signals and, therefore, enhances the activation of T cell. In this study, I elucidated the biological responses of CD137L on (pre)osteoclasts and RANKL on T cells in the context of in vivo interaction between T cells and osteoclasts. RAW264.7. murine monocytic cells, constitutively express CD137L. Ligation of CD137L with anti-CD137L mAb inhibited RANKL-induced osteoclast formation in a dose-dependent manner. Bone marrow cells are expressed CD137L by the treatment with M-CSF. Cross-linking of CD137L abolished M-CSF/RANKL-evoked the formation of multi-nucleated osteoclasts. Both mouse CD4 and CD8 T cells are expressed RANKL following their activation. Ligation of RANKL with OPG, the decoy receptor for RANKL, inhibited both CD4 and CD8 T cell proliferation. These effects were attributed to RANKL-induced apoptosis. These data indicate that CD137L and RANKL on osteoclasts and T cells, respectively provide them with inhibitory signal.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

균형 생육조건하에서 Actinobacillus sp. El-9에 의한 Poly-β-Hydroxybutyrate의 생산

손홍주,이상준 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

토양으로부터 PHB를 생산하는 세균들을 농화배양에 의하여 순수분리하였다. 이들중 주정중류 폐액을 기질로 하여 PHB를 생산하는 균주를 공시균으로 선정하여 형태학적, 배양적, 생화학적 제반 특성을 검토한 결과 Actinobacillus sp.로 동정되었다. 균체생육을 위한 최적 배양온도 및 pH는 각각 30℃, 7.0이었다. 최적 생륙배지의 조성은 포도당 2%, NH_4NO_3 0.15%, Na_2HPO_4·12H_2O 0.25%, KH_2PO_4 0.25%, MgSo_4·7H_2O 0.15%, FeSO_4·7H_2O 0.005%, CaCl_2·2H_2O 0.003%, 미량원소 용액 5 ml/l이었다. Actinobacillus sp. EL-9의 PHB 생산 최적조건은 균형 생육조건(balanced-growth conditions) 즉, 생육 최적조건과 일치하였으며, 보조기질로 propionic acid을 첨가시 PHB/HV copolymer가 합성되었다. Microorganisms growing alcoholic distillery wastes were isolated from soil. Of them, the selected strain EL-9 had a capability of accumulating poly-β-hydroxybutyrate (PHB) when grown in alcoholic distillery wastes. The strain EL-9 was identified as a genus Actinoba-cillus based on the morphological, cultural, and biochemical characteristics. The strain EL-9 was named temporarily as Actinobacillus sp. EL-9. The optimal temperature and pH for cell growth of Actinobacillus sp. EL-9 were 30℃ and 7.0, respectively. The optimal medium compositions for cell growth comprised glucose 2%, NH_4NO_3 0.15%, Na_2HPO_4·12H_2O 0.25%, KH_2PO_4 0.25%, MgSo_4·7H_2O 0.15%, FeSO_4·7H_2O 0.005%, CaCl_2·2H_2O 0.003% and trace element solution 5 ml/l. To investigate the optimal conditions for PHB production, two-stage culture technique was used. The result showed that the conditions for PHB production are identical those for cell growth. When propionic acid was added as a cosubstrate, PHB/HV copolymer was produced.