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비임균성 뇨도염 환자에서 Chlamydia trachomatis 검출방법에 관한 연구 : 배양법 , 효소면역법 및 직접면역형광법의 비교 관찰
김중환,최태열,김춘원 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.3
Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamyd.ia tra.chomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. Ye have studied a sensitivities of culture, chlamydiaxyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2nd sample was used to Chlamydiazyme test and 3rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%) . 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlarnydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyrne and DIF negative cases are 43/85(50.1%) . In summarized, any one positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%) .