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김용준,김병진,유일정,지동범 한국임상수의학회 2002 한국임상수의학회지 Vol.19 No.1
To investigate the usability of frozen canine embryos for embryo transfer in the dog, 19 donors, 3 recipients, and 6 male dogs were used for the experiment. Natural mating or artificial insemination was performed for breeding the bitches in natural estrus. Vaginal smear test along with progesterone titre test were performed to detect the appropriate mating time and the bitches were bred twice during 3-6days following LH surge. Embryo collection was done on 8, 9-11, 12-13 days after the second mating to collect morula and blastocyst. Embryos were frozen using a programmable freezer and preserved in LN2 tank. Embryos were thawed in 37oC water for 15 seconds and transferred into each uterine horn within 30 minutes. Embryos were collected from 13 bitches of 19 donors(68.4%) and the collected embryos were from between 9 and 13 days after 2nd mating. Embryos were produced both by natural mating(60.0%, 9/15) and AI with frozen semen(100.0%, 4/4). Embryos were collected from the donors weighed between 2.5 and 30kg and their age was from 1.5 to 3 years. 52 embryos were collected from 13 donors and the mean number of embryos was four. The stage of embryos was from 2-cell to gastrula and morulae were colledted mostly from 10 to 11 daysafter 2nd mating. Embryos were collected evenly from each uterine horn and the rate of embryo collection for the number of corpus luteum was 83.9%. Embryos were transferred to 3 recipients(morula 8, blastocyst 1, gastrula 8), however, no offspring was produced.

김용준,서세현,유일정,김명철,최종복,길명순 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.2
These studies were performed to investigate what genital diseases could be diagnosed by ultrasonography in the mares associated with infertility. Of 159 thoroughbred breeders(4 to 18 years old), 31 mares which had been considered as the mares with reproductive disorders were examined by ultrasonography during the breeding season. 1. Hematoma in ovarian disordersl endometritis and pyometra in uterine disorders could be diagnosed by ultrasonography. 2.The rate of infertility was 19.5% out of 159 breeders and the rate of uterine disordres was 71.0% among the whole reproductive disorders. 3.Of 31 mares considered as the mares with infertilityi 26 mares were diagnosed by ultrasonography denoting 83.9% of diagnosis rate. 4.The highest average age of mares among the mares with reproductive disorders was 11.7 years in the mares with uterine disorders. The average age of the mares diagnosed as concepted was 7.5 years, which was much lower than that of the mares with reproductive disorders.5.Crushing one of twin vesicles was confirmed by ultrasonography. In conclusions ultrasonography was feasible for diagnosis of the mares with reproductive problems.
소프트웨어 제품 라인 공학 기반의 전자전 소프트웨어 가변성 모델링 연구
김용준,이정우,이충훈,김수진,장효정,허진혁 한국군사과학기술학회 2026 한국군사과학기술학회지 Vol.29 No.2
- Electronic warfare equipment operates in various environments such as fixed-wing, rotary-wing aircraft, ships, and ground systems, and controls different types of sensors and countermeasures based on the concept of equipment operation. As the concept of weapon system operation becomes more advanced, electronic warfare equipment must meet a wide range of requirements, and the software installed on it must be developed on time while ensuring reliability and quality. Software product line engineering is a methodology for efficiently developing and maintaining various software products used in specific domains by systematically managing commonality and variability, which helps reduce development costs and time while improving quality. In this paper, we apply software product line engineering to the electronic warfare software family and model the variability of electronic warfare software.

젖소 사양기술의 자동화를 위한 연구 II. 체온 측정 방법을 통한 질병자동 진단 시스템
김용준,유일정,정길도,한병성,김동원,김명순 한국임상수의학회 1998 한국임상수의학회지 Vol.15 No.2
These studies were performed to find out the possibility of automatic detection of the diseased animal with fever by farmers themselves. Firstly, the body temperature of 331 dairy cows was investigated according to major disease symptoms manifested. Secondly, AD 590 thermometer was used to take the teat temperature of the milking cows to determine the possibility of automatic taking of body temperature while milking. The temperatures of scapha of ear and coccygeal artery part were also taken fur the non-milking dairy cows and Korean native cowl 1. The average body temperature of dairy cows associated with respiratory diseases puerperal disease, or mastitis was higher than normal temperature denoting respectively 39.8,39.6, and $39.3{\circ}C.2.$ The teat temperaure of the milking dairy cows with fever($39.5~39.6{\circ}C$) and the cows with mastitis was respectively 1.02 and 0.56${\circ}C$ higher than that of normal cows. 3. The average teat temperature taken by AD 590 was 33.91, 34.93, and 34.50${\circ}C$ in normal milking dairy cows, cows with fever(39.5~39.6${\circ}C$), and cows with mastitis, respectively. 4. The mean temperatures at scapha and coccygeal part of non-milking dairy cows and Korean native cows were 35.62 and 36.63${\circ}C$, respectively. It was concluded that AD 590 thermometer would be usable for the farmers to automatirally detect the body temperature of dairy cows while milking and subsquently to find the diseased cow with fever and that the scapha of ear and coccygeal artery part of the cattle could be the body parts of simply detecting body temperature of non-milking cattle.
Some Key Factors in a Bead-based Fluorescence Immunoassay
김용준,김혜윤,아칠성,정문연,박선희 한국바이오칩학회 2008 BioChip Journal Vol.2 No.1
In an effort to understand some key factors in a bead-based fluorescence immunoassay, a series of sandwich-type immunoassays were carried out on a glass plate using a latex bead embedded with dye molecules. The fluorescence bead functionalized with an amine functionality was conjugated with an anti- prostate specific antigen (anti-PSA) using glutaraldehyde. The glass surface was functionalized with the amine group using 3-aminopropltriethoxysilane(3-APT). The amine functionality on the glass surface was converted to aldehyde usinglutaraldehyde followed by conjugation with the anti-PSA. A series of sandwich-type immunoassays were carried out by incubating the anti-PSA bead with PSA followed by incubating the PSA/bead complex on the anti-PSA glass surface. The dependency of the fluorescence intensity in the immunoassay was observed to increase when the fluorescence bead concentration was increased from 10-3 mg/mL to 101 mg/mL with some signs of saturation around the range of 100 mg/mL to 101 mg/mL. Also, the fluorescence intensity was observed toincrease when the incubation time of the PSA /anti-PSA bead complex on the anti-PSA glass was increased from 5 minutes to 60minutes. The shear force generated during the washing step was observed to affect the bead-based immunoassay.