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35종 해조류 추출물의 병원성 세균 및 Candida sp. 진균에 대한 항균 활성 평가
김미선 ( Mi Sun Kim ),권경진 ( Kyung Jin Kwon ),이민진 ( Min Jin Lee ),안선미 ( Seon Mi Ahn ),손호용 ( Ho Yong Sohn ) 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 2012 한국미생물·생명공학회지 Vol.40 No.2
In the course of this study aimed at the development of functional food ingredients from seaweeds, the in vitro antimicrobial activities of methanol extracts prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta) were determined against food-borne diseases and pathogenic microorganisms including multi-drug resistant (MDR) Pseudomonas sp. and Candida sp. Based on disc-diffusion assays at 500 g/disc concentration of the methanol extracts, Ishige okamurai, I. foliacea, Sargassum confusum, and S. yamade exhibited strong antibacterial activities in a broad-spectrum, except against Pseudomonas aeruginosa. In addition to the latter four seaweeds, Ecklonia stolonifera, E. cava and Eisenia bicyclis also demonstrated antifungal activity against C. albicans. Among these 8 selected seaweeds, I. okamurai, I. foliacea, and S. yamade exhibited strong hemolytic activity (55-93%) at 500 g/ml against human RBC. Organic solvent sequential fractions using hexane, ethylacetate and butanol, and water residues were prepared from the 8 selected seaweeds and their anti-Candida sp. activities were further determined. The ethylacetate and butanol fraction of I. okamurai, and the hexane fraction of I. foliacea demonstrated antifungal activity against MDR-pathogenic Candida sp. Although the solvent fractions had no activity against MDR-Pseudomonas sp., our results suggest that seaweeds, especially Ishige okamurai, I. foliacea, S. confusum, and S. yamade could be developed as broad-spectrum antimicrobial ingredients.
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방선균이 생산하는 아미노산 대사길항물질, YS-460의 분리 정제 및 특성
박부길 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3
An amino acid antimetabolite named YS-460 was isolated from the culture filtrate of a newly isolated Actinomycetes identified as Streptomyces sp. Fermentation was carried out in the synthetic medium at 30℃ for 5 days. Purification was done by ion exchange resin, active carbon, silica gel column chromatography and obtained 38 mg of pure active substance per liter of the broth. YS-460, an amino acid like substance, has the molecular formula of C_7H_11NO_3. Its structure determined to be furanomycin by spectral analysis. It is active against some bacteria on a chemically defined medium and reversed competitively by L-isoleucine and non-competitively by L-leucine and L-valine.
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제주 연안의 해수로부터 분리한 Cellulase 생산균 Bacillus sp. GC-1과 GC-4의 동정
지원재 ( Won Jae Chi ),박다연 ( Da Yeon Park ),( Uyangaa Temuujin ),이종열 ( Jong Yeol Lee ),장용근 ( Yong Keun Chang ),홍순광 ( Soon Kwang Hong ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.2
GC-1과 GC-4로 명명된 두 종의 그람 양성 박테리아가 제주도 연안해수로부터 동정되었다. 이 두 균주는 16S rRNA 유전자 염기서열 분석과 생리적 특성 분석결과를 토대로 Bacillus 속의 박테리아로 규명되었다. 균주 GC-1의 16S rRNA 유전자 염기서열은 B. tequiliensis와 B. subtilis subsp. inaquosorum의 16S rRNA 유전자 염기서열과 99.91%의 상동성을 보였고, 균주 GC-4의 16S rRNA 유전자 염기서열은 B. altitudinis, B. stratosphericus 및 B.aerophilus의 16S rRNA 유전자 염기서열과 100%의 상동성을 보였다. 그러나 두 균주의 생리학적-유전학적 특성 분석결과, 이들이 계통적 유연관계를 갖는 다른 Bacillus 속의 균주들과 상당한 차이가 있었고, 따라서 조사된 Bacillus 속과는 다른 속에 속할 가능성이 높았다. 이러한 결과는 Bacillus 속이 진화과정 중에 다양한 변종으로 진화되었음을 암시한다. Two Gram positive bacterial strains, designated strain GC-1 and GC-4, were isolated from coastal seawater near Jeju Island in the Republic of Korea. The two strains were identified as members of the genus Bacillus, based on 16S rRNA gene sequencing and data for physiological characteristics analyses. A subtle difference in physiological and genotypical characteristics has led us to designate the strains GC-1 and GC-4. The strain GC-1 showed a 99.91% similarity in 16S rRNA gene sequencing with B. tequiliensis and B. subtilis subsp. inaquosorum and the strain GC-4 showed a 100% similarity in 16S rRNA gene sequencing with those of B. altitudinis, B. stratosphericus, and B. aerophilus. However, both strains exhibited different physiological and genotypical characteristics in many aspects from those of their phylogenetically closest neighbors listed above, which implies that genus Bacillus has diversified into various species during its evolutionary process.
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Egr-1 유전자의 발현 유도물질을 생산하는 항균성 저 영양 세균의 분리 및 동정
윤상홍 ( Sang Hong Yoon ),김동관 ( Dong Gwan Kim ),이영한 ( Young Han Lee ),신순영 ( Soon Young Shin ),권순우 ( Soon Woo Kwon ),이창묵 ( Chang Muk Lee ),강한철 ( Han Chul Kang ),구본성 ( Bon Sung Koo ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.2
전국 경작지 토양에서 분리한 저 영양 세균 3,800주를 대상으로 암 억제유전자의 작동과 관여하는 전사인자인 Egr-1의 promoter 활성화를 유도하는 세 균주(SSG5, SSG6, SSG10)가 Egr-1 reporter assay와 western blotting 분석으로 최종 선발되었다. 이들 선발 균주들은 16S rDNA와 상업용 생화학적 키트(API 20NE, API ZYM, ID 32GN) 분석에 의해 모두 Pseudomonas koreensis인 것으로 최종 동정되었다. 이들 균주들은 Egr-1 발현을 유도하는 물질뿐만 아니라 다양한 세균의(B. subtilis, S. aureus, and L. monocytogenes) 성장을 저해하는 항균물질도 생산하는 것을 확인하였다. The Egr-1 gene is known to be a transcription factor for activating the expression of many tumor-repressing genes. In this study, three strains activating the promoter of the Egr-1 gene were selected, through the use of Egr-1 luciferase reporter assay and western blotting, from amongst approximately 3,800 oligotrophic bacteria isolated from the cultivated soils of various regions within Korea. These strains were identified as Pseudomonas koreensis on the basis of phylogenetic tree analysis of their 16S ribosomal DNA sequences and biochemical characteristics analyses using a variety of commercial kits (API 20NE, ID 32GN, API ZYM kits). In addition, we discovered that these strains produced anti-bacterial activity against Bacillus subtilis, Staphylococcus aureus and Listeria monocytogenes.
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