Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

ICT를 활용한 수학과 교수-학습 방안 연구

진명숙,정권수,엄경련 순천대학교 과학교육연구소 2001 科學과 敎育 Vol.9 No.-

The purpose of thesis is to make the students who are not interested in athematics have interest in mathematics by using information & Communication Technology(ICT), to understand the nathematical principles, to know how close they are to us through data connected with real life and direct manufacturing and experiment of cyberspace, to solve the mathematics for themselves, and to make mathematics teachers develop and make use of ICT teaching-learning manual. Let’s see the following thesis for this purpose. First, it was divided into 8 teaching-learning activity types in connection with characteristics and educational use possibility of Information Technology. It was analyzed into concept, teaching-learning process procedures, and illustration for teaching-learning. Second, gathered and analyzed the scattered internet sites about mathematics, and made use of the intenet sites for teaching and learning mathematics. Third, for effective ICT teaching-learning. I chose the teaching-learning main idea, objectives and teaching-learning activity type. and I checked the aility to use information Technology and then chose the environment and media for information Technology. And then I planned the teaching-learning strategy. Fourth, I developed and applied the teaching-learning model using information Technology, and analyzed the results. This ICT teaching-learning activities brought interests for mathematics, accomplishment motivation, confidence about mathematics, and sense of superiority to the students. In addition to my student became active in mathematics class through this ICT teaching-learning activities.

알코올이 Dynamic Posturography에 미치는 영향

이진춘,고의경,장민호,이병주,백무진,노환중,왕수건,전경명 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.2

정보기술 연계성이 전략적 공급망 능력과 기업성과에 미치는 영향에 관한 연구

김경호,심수진 김천과학대학 2011 김천과학대학 논문집 Vol.37 No.-

The purpose of this paper is to analyze the relationships among information technology, supply chain capabilities, and firm performance. Due to the limitation of the traditional supply chain capabilities focusing on efficiency, strategic supply chain capabilities such as agility, adaptability, and alignment should be acquired in order to create a sustained competitive advantage for a firm. In this paper, it was hypothesized that IT integration within a firm and among firms in a supply chain would be the factors which affect the strategic supply chain capabilities. The questionnaire survey was conducted and data were collected from 129 companies in Korea. The results of PLS Structural Equation Modeling provide the following findings. First, IT integration within a firm affects the strategic supply chain capabilities except alignment. Second, IT integration among firms in a supply chain affects all of the strategic supply chain capabilities. Third, supply agility and supply adaptability affects a firm's performance.

루소의 敎育思想이 現代體育에 주는 意味

진윤수,김경오 忠南大學校體育科學硏究所 2003 體育科學硏究誌 Vol.21 No.1

The purpose of study was to review the influences of Rousseau's educational thought on the current physical education. For the purpose of this study, a through literature search based on identified in an extensive review of Emile and related literature was performed. Objectives, contexts, and methods of physical education, ideal teacher, and ideal student are reviewed within the related literature. According to Rousseau's thoughts, objectives of physical education are to develop the various organic systems of the body through physical activity in the environment so students will respond in a healthful way to the increased demands placed on them. The contexts of physical education for child contain recreation and outdoor activities that can provide them play opportunities to benefit them physically(physical fit), psychologically(hardship), and socially(faire, friendship, cooperation). The contexts of physical education for youths contain physical activity, work, and game that can provide opportunities to develop physical fitness. His teaching method was 'everything according to nature'. The laws of nature dictate to the teacher and to the student the logical pattern of growth and development(five stages). Rousseau was a strong believer in discipline. He felt that in order to create good children, discipline is a must. Rousseau also suggested that teachers let kids think that they are in control, and develop where the child naturally chooses to do well. Rousseau suggested that a teacher must have many different learning experiences. He also stressed that a teacher should have the abilities of understanding the law of nature and kid's growth and development. Most irnportantly, a teacher, by setting a good example can and will greatly influence a student's behavior and overall performance. Rousseau also felt that each student has the right to pursue freedom and happiness. It falls largely onto the teacher to find the way to help the student reach his or her potential for intelligence. Rousseau also mentioned that students should learn from experience and that students are exceptional imitators. The purpose of physical education, according to Rousseau was to develop all the child's abilities freely, as nature intended. In Rousseau's view, nature was the primary teacher and the job of the "tutor" was to be a guiding force. In Rousseau's naturalism, exploration of one's capabilities and interest leads directly to greater skills and adjustments to nature. Rousseau regarded physical activity as important, it was body, mind, and knowledge. Through physical activities, the individual develops in an all-around way.

T-scan을 이용한 교합분석에 관한 연구

진태호,한경수 圓光大學校 1993 論文集 Vol.27 No.2

Occlusion should be evaluated in view of the normal physiologic individual variations, and properly treated for good occlusal conditions that have harmonious that harmonious interrelation to masticatory functions. To do so, thorough knowledge and close examination on dental morphology, occlusal contacts during excursions, the mandibular movements, masticatory muscles and tempormandibular joint are essential to vevry clinician. Occlusal contacts during movements is as much important as other components or parameters above mentioned because it reflets not morphohologic status but functional dynamic demands and abilites of masticatory system. The objectives of this study was to investigate and analyze the effects of chewing side and lateral guidance pattern on the occlusal contacts. The author selected 65 students in Wonkwang University Dental College who had not any mandibu-lar dysfunction, and no extensive dental treatment history and also selected 60 patients visiting dentalinfirmary with temporomandibular disorder. They classified by chewing side preference and lateral guidance pattern into 4 groups. To record occlusal tooth contacts, T-Scan System (Tekscan Inc., U,S,A) was used on maximal clenching Record was done 3 times for accuracy, for every subject. The data was analyzed on 3 items which were number of tooth contact points, distribution of early contact points and average contact time intervals, and then, tested statistically. The obtained results were as follows: 1. The range of contact points on both sides in all groups were from 28 to 32. Bilateral canine guidance group had the most contact points, and unilateral non-canine guidance group had the least. Statistical difference between right and left side(or ipsilateral and contralateral side) were noted inallgroups. 2. In normal group, the degree of accordance of early contact points with preferred chewing side was higher in non-canine guidance groups, which was more prominent in case combined with uniateral chewing. And in patient group, result of canine guidance group was the same as normal group but that of the non-canine group was contrary to normal group. 3. Average contact time intervals in normal group were from 0.015 to 0.019sec, and showed no statistical difference among 4 groups. But, there was a tendency that the intervals were increased with canine guidance and/or unilateral chewing pattern. And average contact time intervals in patient group were higher than that of normal group as from 0.037 to 0.044sec, but there was no significance statistically.