치매환자에 대한 Indeloxazine hydrochloride의 임상효과

한상익,백인호,전태연,고효진,김용찬 최신의학사 1991 最新醫學 Vol.34 No.3

드론 에너지원 전환의 한계 : 규제와 인증을 중심으로

한상익 한국디지털정책학회 2022 디지털정책학회지 Vol.1 No.2

농업 생산성 향상과 소비활성화 촉진 전략 -밭작물 자급율 향상과 소비활성화 현장 확산-

한상익,이수경,배진우,문윤만,전형광,임시규 한국행정학회 2016 한국행정학회 학술발표논문집 Vol.2016 No.-

Application of SSR Markers and Real-time PCR for Variety Identification in Azuki-bean [Vigna angularis (Willd.) Ohwi and Ohashi]

한상익,Deuk-Yong Suh,하태정,이명희,Woo-Duck Seo,오성환,장기창,Je-Bong Lee,박금룡,강항원 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.4

The azuki bean in Korea consists of seven domestic varieties which have been developed and registered for the public during last 25 years. Here, we present a simple but reliable method to screen and identify Korean azuki bean varieties. A method based on simple sequence repeat (SSR) markers is widely used for prominent gene identification and variety discrimination. In molecular biology, real-time polymerase chain reaction (PCR) is a laboratory technique based on the polymerase chain reaction that is used to amplify and simultaneously quantify a targeted DNA molecule. It enables easy detection of a specific sequence in a DNA sample without performing electrophoresis and further processes. For separation of seven Korean azuki bean varieties, 110 unique azuki bean SSR markers from an (AG)n-enriched library were selected, synthesized and used for polymerase chain reaction (PCR). Data were taken through acrylamide gel electrophoresis and automated multi-capillary electrophoresis system for selection of specific markers and then changed into proper formats for data mining analysis. Ten primer pairs that showed high polymorphism were chosen for the indepth study. These ten primers were re-amplified with real-time PCR and checked the cycle threshold (Ct) and temperature (Tm) for comparison of amplification sequence in seven varieties. Consequently, a total of 20 alleles and 6 SSR primers were detected from the standard PCR amplification. Within these 6 primers, 7 alleles of 3 SSR primers were isolated for variety identification. From real-time PCR results, 3 SSR primers were selected as efficient markers for discrimination of seven Korean azuki bean varieties. The approach described here could be applied in monitoring our varieties and can be adapted in the azuki bean breeding program. The azuki bean in Korea consists of seven domestic varieties which have been developed and registered for the public during last 25 years. Here, we present a simple but reliable method to screen and identify Korean azuki bean varieties. A method based on simple sequence repeat (SSR) markers is widely used for prominent gene identification and variety discrimination. In molecular biology, real-time polymerase chain reaction (PCR) is a laboratory technique based on the polymerase chain reaction that is used to amplify and simultaneously quantify a targeted DNA molecule. It enables easy detection of a specific sequence in a DNA sample without performing electrophoresis and further processes. For separation of seven Korean azuki bean varieties, 110 unique azuki bean SSR markers from an (AG)n-enriched library were selected, synthesized and used for polymerase chain reaction (PCR). Data were taken through acrylamide gel electrophoresis and automated multi-capillary electrophoresis system for selection of specific markers and then changed into proper formats for data mining analysis. Ten primer pairs that showed high polymorphism were chosen for the indepth study. These ten primers were re-amplified with real-time PCR and checked the cycle threshold (Ct) and temperature (Tm) for comparison of amplification sequence in seven varieties. Consequently, a total of 20 alleles and 6 SSR primers were detected from the standard PCR amplification. Within these 6 primers, 7 alleles of 3 SSR primers were isolated for variety identification. From real-time PCR results, 3 SSR primers were selected as efficient markers for discrimination of seven Korean azuki bean varieties. The approach described here could be applied in monitoring our varieties and can be adapted in the azuki bean breeding program.

눈큰흑찰’의 가바(GABA) 함량 증진을 위한 이화학적 처리 효과

한상익,나지은,서경혜,박지영,서우덕,박동수,조준현,이종희,심은영,남민희 한국작물학회 2014 한국작물학회지 Vol.59 No.4

We assessed the GABA accumulation and othercomponents after the ‘Nunkeunhukchal (BGE)’, ‘Josanghukchal(BR)’, and ‘Ilmibyeo (IB)’ grain was soaked in water for24, 36, 48, 60, 72 and 96 hr. The results showed a continuousaccumulation of GABA in soaking treated brown rice ofBGE and IB. Among the treated hours, 72 hours of soakinghad the maximal accumulation of GABA (51.4 mg/100 g),amino acid, polyphenol and other components. The activitiesof glutamate decarboxylase (GAD) in brown rice and rice-branwere the same in BGE rice. However, the formation of GABAtreated with L-glutamate as substrate showed dramatic increaseof 354.6 (fourteen times higher than normal extraction) and726.4 mg/100 g in BGE rice and rice-bran, respectively. These results suggested that the soaking and extraction withL-glutamate buffer could be better methods for the harvestof increased GABA.

벼 부산물을 함유한 생분해성 멀칭비닐의 포장 재배조건에서의 특성 및 분해력 연구

한상익,강항원,장기창,서우덕,오성환,나지은,이형운,정미남,최경진 한국작물학회 2012 한국작물학회지 Vol.57 No.2

The main challenges for the development of agricultural bio-degradable mulching film concern the degradation during the lifetime of cultivated crops. A set of rice by-product (rice-hull and rice-bran) based bio-degradable mulching films was developed and tested, following the measurement of standard bio-degradability rate and adaptability in a large scale field experiment. The standard bio-degradability of bio-film passed the KS (Korea standard) regulation. The result of mechanical analysis of bio-degradable mulching film presented a higher mechanical strength and elongation rate compared with polyethylene film. In addition, bio-film could be degraded into fragments within 4 months under the field condition of several upland crops. Bio-degradable mulching film indicated great potential as a new source of agricultural bio-degradable material. 작물의 멀칭재배에 활용이 가능한 생분해성 비닐의 생분해 특성과 작물 재배에 따른 특성 변이를 검정한 결과는 다음과 같다. 1. 천연고분자인 벼 부산물(왕겨와 쌀겨)을 이용하여 상업적으로 이용이 가능하고 생분해가 가능한 멀칭 비닐을 제조하였다. 2. 생분해성 멀칭 비닐의 특성 검정을 위한 표준 생분해도 측정결과 셀룰로오스 대비 75%의 생분해가 되어친환경 기준을 충족함을 확인하였다. 3. 생분해성 멀칭 비닐의 기계적인 특성은 기존 멀칭비닐과 비교해서 인장강도와 인장력에서 우수함을 보였고,파단점 비후 비교에서도 생분해성 비닐은 지속적인 힘을 유지시켜야 늘어나는 특성을 보였다. 4. 작물을 대상으로한 시험에서 기계화도 가능하며, 인장강도와 인장력을 통한 분해력 비교에서 작물에 따라시간이 지남에 따라 인장강도와 인장력에 차이를 보임을 관찰할 수 있었고, 작물의 토양 피복도가 생분해에영향을 미침을 확인하였다.

카나다의 맥주보리 생산 현황과 품종육성 및 양조

한상익 한국맥류산업발전연구원 2003 한국맥류연구회지 Vol.10 No.-

위급상황 통신 지원용 공중 통신중계기의 빠른 배치 기법

한상익 한국정보통신학회 2023 한국정보통신학회논문지 Vol.27 No.1

An aerial relay system utilizing an unmanned aerial vehicle(UAV) or drone is addressed for event-driven operations such as temporary communication services for disaster affected area, military and first responder support. UAV relay system (URS) targets to provide a reliable communication service to a remote user equipment or an operator, therefore, a fast UAV placement to guarantee a minimum quality of service(QoS) is important when an operation is requested. Researches on UAV utilization in communication systems mostly target to derive the optimal position of UAV to maximize the performance, however, fast deployment of UAV is much more important than optimal placement under emergency situations. To this end, this paper derives the feasible area for UAV placement, investigates the effect of performance requirements on that area, and suggests UAV placement to certainly guarantee the performance requirements. Simulation results demonstrate that the feasible area derived in this paper matches that obtained by an exhaustive search. 무인항공기 또는 드론(UAV or Drone)을 활용한 공중 통신중계기는 재난피해 지역, 군 작전 지역, 응급상황 발생 시 등의 임시 통신 서비스 지원에 활용되고 있다. 무인항공기를 활용한 공중 통신중계기는 원격 사용자 또는 임무 수행자에게 안정적인 통신 서비스를 제공하는 것을 목표로 하므로 최소 성능 요구사항을 보장하는 빠른 무인항공기의 배치가 중요하다. 무인항공기를 통신 시스템에서 활용하는 연구에서는 통신 성능을 극대화하는 무인항공기의 최적 위치를 찾는 연구가 대부분이나, 위급상황 시에는 최적 위치보다는 무인항공기 배치 가능 영역을 빠르게 도출하고 통신 지원을 위한 빠른 배치가 더 중요하다. 본 논문에서는 무인항공기를 공중 통신중계기로 활용 시 각 상황에 따라 요구되는 통신 성능을 보장하는 무인항공기 배치 가능 영역을 도출하고, 이론적 분석을 통해 얻은 결과를 시뮬레이션 완전 탐색을 통해 검증하고자 한다.

한국 대통령선거에서 정치 외부자(political outsider)의 도전

한상익 한국정치사회연구소 2022 한국과 국제사회 Vol.6 No.1

After democratization, Korea held seven presidential elections, with seventeen out of a total of 54 candidates winning significant votes of 5% or more. Fourteen of the 17 people, are political insiders who have gained political prominence through political parties or public office careers, and the other three men are in line with the conceptual characteristics of political outsiders in that they ran for president based on popular reputation gained outside of politics. A political outsider refers to a person running for election with a popular reputation gained outside of politics without any political party or public office careers. They appear mainly based on distrust politics itself or political party, and they emphasize post-ideological centrism and pragmatic politics. The three political outsiders who challenged the Korean presidential election show the characteristics of political outsiders not only conceptually but also behaviorally. Considering the reality in Korea where political distrust is very high and direct communication through digital media is expanding, political outsiders are likely to emerge not only in third party candidates but also in mainstream political parties. 민주화 이후 한국은 7회의 대통령 선거를 치렀으며 총 54명의 후보중 17명이 5% 이상의 유의미한 득표를 얻었다. 17명 중 14명은 정당이나 공직 경력을 통해 정치적 지명도를 얻은 정치 내부자이며 나머지 3명은 정치 외부에서 얻은 대중적 명성을 기반으로 대선에 출마했다는 점에서 정치 외부자의 개념적 특성에 부합한다. 정치 외부자는 어떠한 정당 활동이나 공직 경력 없이 정치 외부에서 얻은 대중적 명성을 가지고 선거에 출마하는 자를 뜻한다. 이들은 정치 또는 정당 불신을 기반으로 주로 등장하고 탈이념적인 중도와 실용적 정치를 강조하며 대중영합적인 소통 방식과 정책 공약을 그 행태적 특징으로 한다. 한국 대선에 도전한 3인의 정치 외부자는 개념적 특성뿐만 아니라 행태적으로도 정치 외부자의 특징을 나타내고 있다. 정치 불신이 매우 크고 디지털 미디어를 통한 직접 소통이 확대되고 있는 한국 현실을 감안할 때, 이후 제3후보만이 아닌 주류 정당에서도 정치 외부자가 등장할 가능성이 높다.

번식 육종 1 ; 체외성숙 우 난포란의 체외수정에 미치는 Caffeine 과 Heparin 의 영향

한상익,박춘근,양부근,김정익 한국동물자원과학회 ( 구 한국축산학회 ) 1991 한국동물자원과학회 심포지움자료집 Vol.- No.-