Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

사람 갑상선자극호르몬 수용체를 발현하는 Chinese Hamster Ovary(hTSHR-CHO)세포를 이용한 자극형 갑상선자극호르몬 수용체 항체 활성 측정의 임상적 유용성

한희정,원진호,채수홍,김현진,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.1

Detection of thyroid stimulating hormone receptor(TSHR) autoantibodies has clinical value in establishing the diagnosis of Graves ' disease, and in predicting its clinical course with high sensitivity and high specificity. Especially, TBII using radioreceptor assay has been widely used. Recently, for detecting thyroid stimulating antibody(TSAb), a sensitive method using human TSH receptor-transfected Chinese Hamster Ovary(CHO) cells has been developed and clinically used. The aims of this study are to determine the change and discrepancy of 2 TSHR autoantibodics in clinical course and to evaluate the correlation between 2 autoantibodies. We measured TSAb and TBII in 30 newly diagnosed and untreated patients and in 73 previously diagnosed as Graves ' disease and treated with antithyroid drug. Also we compared the changes of the antibodies in clinical course and correlation between 2 antibodies. Thes results are summarized follows. 1. In newly diagnosed patients, all of the 30 patients had positive TBII and/or TSAb. None of them had negative TBII and negative TSAb. And in newly diagnosed patients, mean TBII and mean TSAb values are 46.8% and 361%, respectively. In 73 treated patients mean values are 18. 7% and 196% each other. Therefore, newly diagnosed group has significant greater TBII and TSAb than antithyroid drug-treated group. 2. TBII was significantly different between euthyroid group and thyrotoxic group in antithyroid drug-treated patients, but TSAb was not. 3. TBII was sequentially decreased as increased medication duration, but TSAb was not shown sequential decrease as increased medication duration. 4. There was significant negative correlation between cumulative doses of antithyroid drug and TBII, but no significant correlation between cumulative doses and TSAb. 6. There was significant positive correlation between TBII and TSAb in total patients group and in antithyroid drug medication group, but not in newly diagnosed group. In conclusion, in Graves ' disease patients, detection of TSHR antibodies are clinical values in establishing the diagnosis and prediction of clinical course. A method detecting TSAb using hTSHR-CHO cells should be further studied for standardization of measurement, and for detecting the changes of TSAb in clinical course, long-term follow-up of patients are important.

호텔 고객 테이터베이스 마케팅의 활용전략 방안 연구

한진수,박향일 경희대학교 관광산업정보연구원 2000 觀光産業情報論集 Vol.2 No.-

Customer expect to be serviced better and more precisely, and competition is trying even harder to capture market-share from other companies. Mass-Marketing no longer works: customized and indivisually tailord marketing efforts are necessary. This study is to rethink the role of the database marketing by analyzing the case of three deluxe-class hotels in Korea. It's focused on a relationship with customer, so generically we call it 'Customer Database Marketing' in this study.

시민참여와 정부신뢰

정수현,강한솔,황은진,이정주,노승용 서울여자대학교 사회과학연구소 2012 사회과학논총 Vol.19 No.-

이 연구는 시민참여와 정부신뢰와의 관계를 분석하는데 그 목적이 있다. 이를 위하여, 이 연구에서는 시민참여를 전통적인 오프라인 시민참여와 디지털 방식인 온라인 시민참여로 그 유형을 분류하고, 오프라인 시민참여와 온라인 시민참여의 경험이 정부신뢰와 어떤 관계가 있는지를 분석하였다. 2차 자료를 활용하여 빈도분석, 교차분석, t-test, 분산분석, 회귀분석 등을 실시한 결과 먼저, 우리나라 시민참여 경험자는 약 22% 정도이며, 오프라인 시민참여 경험자는 약 12%, 온라인 시민참여 경험자는 약 15%, 그리고 온라인과 오프라인 시민참여를 모두 경험한 시민은 약 5% 정도인 것으로 나타났다. 또한, 시민참여와 정부신뢰와는 관계를 분석한 결과 통계적으로 유의미한 관계가 없는 것으로 나타났다. 오히려, t-test 및 분산분석 결과 오프라인 및 온라인 시민참여 경험자에 비하여 무경험자의 정부신뢰 수준이 상대적으로 약간 더 높은 것으로 나타났다. 시민참여의 경험 유무 만으로 정부신뢰를 설명하는데는 한계가 있음을 보여 주는 결과일 뿐만 아니라 시민참여를 제도적으로 보장하는 것에서 나아가서 시민참여가 실질적 효과를 높일 수 있도록 시민참여과정에서 정부와 시민의 보다 적극적인 노력이 필요함을 보여주는 결과라 하겠다. Trust in government adds legitimacy to political system, provides social stability, and helps government to work effectively. In addition it increases cooperation between governments and citizens, and improves effectiveness of government affairs. However, citizens' trust in government has been quite low sine 1990s. In Korea, citizens' trust in government dropped after financial crisis in late 1990s. Various literatures indicated that citizen participation is one of the best ways to increase citizens' trust in governments. Especially as information technology increases, it is expected that citizens acquire information about different policy areas, and actively participate in surveys, discussion, and policy evaluation through internet. This research attempts to prove the relationship between citizen participation and trust in government by comparing online and offline citizen participation. The research utilized 2nd dataset from 'the influence of information on citizens' participation in policy-making.' With various statistical techniques such as frequency analysis, cross tabulation, ANOVA, and regression analysis, the characteristics of offline and online policy participants are examined. The results showed that citizens, who participate in policy-making processes more actively both online and offline, trust in government less. Comparing online and offline participation, online participants distrusted government more than offline participants. Such variables as policy competitiveness, democracy in government, quality of government, and transparency in government have direct influences on trust in government. However, citizen participation is not statistically significant. Key Words:Citizen Participation, Traditional Citizen Pariticipation, Digital Citizen Participation, Trust in Government.

운동성 산화 스트레스와 항산화비타민의 보충이 말초단핵세포의 NF-kB 활성에 미치는 영향

진영수,박건구,박준영,김미정,이왕록,김혜영,이한준,박은경 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Reactive oxygen species(ROS) are implicated in the pathogenesis of a wide variety of human diseases. Numerous studies indicate that ROS may serve as common intracellular molecules that contribute to the process of nuclear factor(NF)κB activation in response to a diverse stimuli. In our laboratory, we have demonstrated tat antioxidants could reverse the decline of immune function caused by exercise-induced ROS. Furthermore, it in necessary to understand a mechanism underlying ROS-dependent disorder in biological system. Recent studies have been shown that several gene expression were regulated by oxidants, antioxidants and other determinants of the intracellular reduction-oxidation(redox) state. In this process, NF-κB have been shown to play a important role. The purpose of the present investigation was to evaluate the effect of exercise-induced oxidative stress and antioxidnt supplementation on NF-κB activation in peripheral mononuclear cells. Forty male SD rats(4 weeks old) were randomly divided into noraml diet group and antioxidants(ATO) supplement group, and then ATO groups were treated with antioxidants(VE: 2001U/kg, VC: 50mg/rat, β-carotene: 300mg/kg, vitamin B6: 250㎍/100g, selenomethionine: 0.1mg/kg) for 16 weeks. After 16 weeks breeding at each condition, each group divided into two groups: Control group(CR) fed generally, Exercise group(CE) fed generally and followed by acute exercise 16 weeks later. Antioxidant Antioxidants and Exercise group(AE) fed with antioxidants and followed by acute exercise. The activation of NF-κB binding activity was increased after exhaustive exercise bout in both group. In addition, pretreatment of ATO group with the antioxidants mixture lead to the inhibition of NF-κB binding activity. This results suggest that NF-κB activation should be further studies in response to a variety of exercise.

小豆 成分育種을 위한 主要化學成分의 遺傳分析

韓鏡秀,張權烈,金鎭馨 慶尙大學校 1985 論文集 Vol.24 No.2

小豆 成分育種올 위해 主要化學成分의 遺傳關係를 早期에 推定하고 앞으로의 育種計劃에 대한 基礎資料를 얻고자, F_l, F_2 兩世代의 優性程度 및 遺傳子 分布狀態, 그리고 遺傳力과 相關係數等을 推定한 바, 그 結果는 다음과 같다. 1. 優性程度는 全成分에서 各各 超優性으로 遺傳되었으나 Mg는 不完全優性(F_l世代)과 超優性(F_2世代)으로 世代間에 差異가 있었고, F_l 世代의 粗脂肪, 灰分및 Mg와, F_2 世代의 灰分에서는 非對立遺傳子의 關與가 多少 있었다. 2. 各 成分의 遺傳力은 兩世代間에 비교적 낮았으나 K와 粗脂肪은 他成分보다 높았다. 3. 形質相互間의 相關關係에서 灰水化物은 他成分間과 負의 相關을 보였고, 기타 成分들은 相互 正의 相關이었는데, 蛋白質과 粗脂肪은 F_2世代에서 有意한 正의 相關을 나타내었다. A set of 36 crosses of F/s and F_2's from 9 X9 diallel crosses of nine azdukibean varieties were analyzed for estimating genetic parameters, such as gene action, degree of dominance and genotypic correlation. Seven chemical com positions, i. e., carbohydrate, protein, crude fat, ash, K, Ca and Mg were observed. The results obtained are summarized as follows: 1. In Vr-Wr graphical analyses, all chemical compositions except Mg in F/s were found to be inherited in over dominance. 2. The highest heritability value was found in K in both F/s and F/s, but the value of ash was the lowest. 3. The genotypic correlations between carbohydrate and other chemical compositions were negatively correlated, but that between protein and crude fat was positively correlated in F_2's.

간호사의 정신과 자문에 대한 기대도

韓辰熙,房勝規,金光壽,柳太烈 최신의학사 1991 最新醫學 Vol.34 No.1

小豆의 交酸集團에 따른 世代別 遺傳子 分布狀態 및 組合能力의 檢定

韓鏡秀,張權烈,金鎭馨 慶尙大學校 1985 論文集 Vol.24 No.1

The two sets of diallel crosses (5×5 and 9×9 crosses) from nine adzukibean varieties (Yeongdong Jeogdu, Gongju Baegdu, Gyeonggi Gaeguri, Wase Dairuyu No. 1, Natsu Adzuki, Maruba No. 1, Jeogdu, Nesumi Mochi and Hongcheon Jeogdu) were grown in the field for estimating genetic parameters, such as gene action and combining ability in two different generations, F_1 and F_2. From a series of experiments conducted in 1976 to 1977, 1981 to 1982, and two agronomic characters, i.e., days to flowering and 100-grain weight were observed. The results obtained are summarized as follows: 1, In finks and Hayman's (Vr-Wr) graphic analyses, days to flowering and 100-grain weight of F_1's and F_2's from 5×5 and 9×9 crosses were found to be inherited in partial dominance. 2. Mean squares of GCA and SCA were significantly different among two characters in all generations of different crosses, and those of GCA were higher than SCA. 3. The highest GCA effects for days to flowering were expressed Jeogdu in 5×5 cross, Hongcheon Jeogdu in 9×9 cross, ana Jeogdu for 100-grain weight showed relatively high effects in two generations of 5×5 and 9×9 crosses. 4. SCA effects were different among Parents and characters in the different crosses, but the higher SCA effects were exhibited in the hybrid between Yeongdong Jeogdu and Nesumi Mochi in two generations of 5×5 cross.