Alcohol extract of Geum Chung Cho alleviates alcohol-induced short-term memory impairment in gerbils

Han-Sam Cho,Tae-Woon Kim,Sang-Seo Park,Mal-Soon Shin,Jae-Min Lee,Eun-Sang Ji,Chang-Ju Kim 한국운동재활학회 2015 한국운동재활학회 학술대회 Vol.2015 No.10

Chronic ethanol consumption leads to memory impairment and also induces brain damage. The hippocampus has been particularly regarded as a possible target organ of alcoholic intoxication, since this structure plays a prominent function in memory processes. Cordyceps has been used as a traditional medicine in Asian countries for a long time. Different types of Cordyceps extract were reported to have various pharmacological activities including anti-tumor, anti-inflammatory, anti-viral, and immune modulatory effects. Guem Chung Cho is an artificial cultivated Cordyceps, and was supplied from GCordy (Seoul, Korea). Ethanol extract of Cordycep (50 g) was made by rotary evaporate, and resulting powder 8.29 g was obtained (yield 17%). In the present study, we investigated the effects of ethanol extract of Geum Chung Cho on short-term memory, cell proliferation and apoptosis in the hippocampus of ethanol-treated gerbils. The gerbils were divided into 5 groups (n = 10 in each group): Control group, alcohol-treated group, alcohol-treated and 0.001 mg/kg Geum Chung Cho-applied group, alcohol-treated and 0.01 mg/kg Geum Chung Cho-applied group, and alcohol-treated and 0.1 mg/kg Geum Chung Cho-applied group. Gerbils in the alcohol-treated groups were orally administered ethanol (1 g/kg) once a day for 2 weeks. Gerbils in the Guem Chung Cho-applied groups were orally administered Guem Chung Cho extract at each dosage 1 hour before ethanol consumption. Our results revealed that consumption of alcohol for 2 weeks deteriorated short-term memory, induced apoptosis, and inhibited cell proliferation, and reduced expressions of brain-derived neurotrophic (BDNF) and tyrosine kinase B (TrkB). However, Guem Chung Cho treatment alleviated alcohol-induced short-term memory impairment through enhancing cell proliferation and BDNF expression with inhibiting apoptosis in the hippocampus. Guem Chung Cho showed maximum effects at 0.1 mg/kg. Based on these results, alcohol extract of Guem Chung Cho may provide therapeutic value for the treatment of chronic alcohol-inducted brain injury. This study was supported by GCordy company.

AHP를 이용한 CT 및 MRI의 비용편익분석

조근태,김성조,김성민,김용우,김성재,조용곤 한국경영과학회 2004 經營 科學 Vol.21 No.2

The imports of used medical equipment such as CT, MRI have been rapidly increased every year. How to manage those medical equipment has been one of critical issues in the government sector. In this study, we demonstrate how benefit/cost analysis using the Analytic Hierarchy Process (AHP) can be applied to the comparison of used CT/MRI and new CT/MRI. The final results show that the new CT/MRI is more attractive than the used CT/MRI.

Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Cyclophosphamide 투여 흰쥐에서 비타민 A가 창상파열력에 미치는 영향에 관한 실험적 연구

조해석,정상훈,임풍,김진철,안상태 大韓成形外科學會 1985 Archives of Plastic Surgery Vol.12 No.1

Cyclophosphamide lays restraints upon production of fibroblast and collagen fiber and reduce wound burst strength by acting on fibroblast in acute inflammatory phase. Vitamin A is known to increase inflammatory reaction by exerting an effect upon inflammatory cells in the beginning of the wound healing process. It shows contrary effect to cyclophosphamide. In order to study the influence of vitamin A over wound healing delay induced by cyclophosphamide in rats, we observed and compared the wound burst strength of cyclophosphamide and vitamin A combined treated group with those of control and cyclophosphamide treated group. Wound burst strength of each group was measured in the skin segments of 2×4cm obtained from the operation sites at the 3rd, 5th, 7th and 14th postoperative days after making 5cm incision and suture at the back of 72 rats. Results of the experiment are as follows; 1. Wound burst strength in control group increases slowly as days go by and shows trends of sudden increase between the 5th and 7th postoperative days. 2. Wound burst strength in cyclophosphamide treated group is markedly less than that in control group. 3. Wound burst strength in cyclophosphamide and vitamin a combined treated group shows similar level to that in control group. 4. Body weight in cyclophosphamide treated group reduces gradually as days go by. But there's no weight reduction in cyclophosphamide and vitamin A combined treated group. Judging from these results, we found the fact that vitamin A could prevent or recuperate the side effects of cyclophosphamide, delay of wound healing and worsening of general condition.

상아질에 대한 저점도 복합레진의 자가접착에 관한 연구

조태희,최경규,박상혁,박상진 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.3

The objectiveness of this study was to evaluate whether low-viscosity composite can bond effectively to dentin surface without bonding resin. The low-viscosity composites being 50wt% filler content were made by the inclusion of bonding resin of two self-etching systems(Clearfil SE Bond, Unifil Bond) varied with contents as 0, 10, 20, 30, 40, 50wt%. Exposed dentin surfaces of extracted 3rd molars are used. Dentin bond strengths were measured. The tests were carried out with a micro-shear device placed testing machine at a CHS of 1mm/min after a low-viscosity composite was filled into an iris cut from micro tygon tubind with internal diameter approximately 0.8mm and height of 1.0mm. 1. Flexural strength and modulus was increased with the addition of bonding resin. 2. Micro-shear bond strength to dentin was improved according to content of bonding resin irrespective of applying or not bonding resin in bonding procedure, and that of Clearfil SE Bond groups was higher than Unifil Bond. 3. There were no significant difference whether use of each bonding resin in bonding procedure for S-40, S-50, U-50(p>0.05). 4. In SEM examination, resin was well infiltrated into dentin after primed with self-etching primer only for S-50 and U-50 in spite of the formation of thinner hybrid layer. Low viscosity composite including some functional monomer may be used as dentin bonding resin without an intermediary bonding agent. It makes a simplified bonding procedure and foresees the possibility of self-adhesive restorative material.

Compatibility를 이용한 다수 전문가의 가중치 종합화에 관한 연구

조성훈,김태성,이영찬 한국경영과학회 1998 韓國經營科學會誌 Vol.23 No.4

The objective of this study is to propose a new procedure to synthesize the multi-experts priorities in AHP. if multi-experts with different expertise are involved in a AHP decision, we need some way to aggregate their opinions. AHP model used to do numerical aggregation by taking only the geometric mean or the weighting geometric mean in past. To aggregate the multi-experts priorities, in this paper, we suggest a way which Decision Maker can exclude outlier matrix from group using the concept of the Compatibility and we introduce Delphi method to use Compatibility in AHP. A numerical example is shown to illustrate the procedure.

충주 온천지역의 관광환경자원의 과학적 평가 : 타 온천 지역과의 대기오염상태 비교 Comparison of Atmospheric Environmental State

조일환,전태성,허억 건국대학교 의과학연구소 2003 건국의과학학술지 Vol.13 No.-

Since the concept of clean air at hot spring resorts in Chungju city depends upon the scientific evidence of air pollution state, the atmospheric data at this city should be compared with other cities. The aim of this study was to investigate a degree of atmospheric cleanness at Chungju city comparing with other ones. The measurement results at this city showed the concentration of sulfur dioxide (S0_2; 0.0043 ppm/yr ~ 0.04 ppm/yr), total suspended particulate(TSP; 38.5 ㎍/m^3/hr ~ 90 ㎍/m^3/hr), ozone(0_3; 0.0133 ppm/hr ~ 0.0267 ppm/hr), nitrogen dioxide(N0_2; 0.0078 ppm/yr ~ 0.037 ppm/yr), and carbon monoxide(CO; 3.9 ppm/hr - 21.2 ppm/hr). The atmospheric cleanness at Chungju is involved in high group (p<0.05; high group vs low group) among the hot spring resort cities - Changwon, Seoul, Pusan, Soowon, Taejon, and Kangrung. As Chungju has a lake and less developed manufacturing area, the establishments of frontier industry, the leisure industry, the spa-medical industry and education facilities are strongly recommended for the municipal policy of the city.

점적상에서의 수력학 특성

조지훈,성기천,함병호,김태옥 明知大學校 産業技術硏究所 1991 産業技術硏究所論文集 Vol.10 No.-

Hydrodynamics was investigated in a trickle bed with spherical particles. Flow transition boundary, pressure drop, and liquid holdup were measured and analyzed in an air-water system. The results showed that liquid velocity at trickle-pulse transition boundary increased with increasing packing size and it decreased with increasing gas velocity, and that the theoretical model proposed was well applicable to predicted flow transition boundary. In the same operation conditions, flow characteristics was changed with operation mode because of liquid closslinking. Therefore, as the maximum gas velocity increased, pressure drop increased. Also, pressure drop increased with increasing fluid velocity and it increased with decreasing packing size, and the effect of gas velocity on pressure drop for higher liquid velocity was less than that for lower liquid velocity. Liquid holdup increased with increasing liquid velocity and it decreased with increasing packing size, and the influence of gas velocity on liquid holdup for pulse flow regime had larger than that for trickie flow regime. By contrast, the influence of packing size on liquid holdup for pulse flow regime had less that for trickle flow regime. In this conditions, correlations of pressure drop and liquid holdup were proposed in terms of Reynolds numbers of total fluids and liquid phase.