Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가

이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.

췌장 선방세포암 1예

이화정,지준호,박승찬,박정철,최은정,서혜진,이원식,이정림,배병조,손경락,이경희 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Acinar cell carcinoma is a rare tumor that represents 1~2% of al1 pancreatic cancers. Clinical and radiologic findings are inconclusive in this disease Acinar cell carcinoma is characterized by rapid progression and early metastasis, which lead to its poor prognosis. A 41-year-o1d man was admitted to our hospital for abdominal pain. Abdominal computed tomography (CT) and positron emission tomography-computed tomography (PET-CT) showed a splenic mass, which was being invaded by a pancreatic tail mass and which had increased ^(18)F-fluorodeoxyglucose (FDG) uptake Primary radical distal pancreatectomy and splenectomy were performed. Pathologic findings revealed an acinar cell carcinoma of the Pancreas The patient underwent a total gastrectomy three months later because of gastric recurrence Four months later, multiple hepatic metastases were discovered, and the patient underwent a left hepatectomy During treatment with capecitabine, there was no evidence of tumor progression for 14 months. We report a case of metastatic pancreatic acinar cell carcinoma, which did not progress for an extended period while the patient was being treated with capecitabme.

구강외과 수술용 스텐트 기반 영상유도 수술 시스템의 개발

이우진,김대승,이원진,이삼선,최순철,허민석,허경회,김명진,이지호 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.3

Purpose : The purpose of this study was to develop a stent-based image guided surgery system and to apply it to oral and maxillofacial surgeries for anatomically complex sites. Materials and Methods : We devised a patient-specific stent for patient-to-image registration and navigation. Threedimensional positions of the reference probe and the tool probe were tracked by an optical camera system and the relative position of the handpiece drill tip to the reference probe was monitored continuously on the monitor of a PC. Using 8 landmarks for measuring accuracy, the spatial discrepancy between CT image coordinate and physical coordinate was calculated for testing the normality. Results : The accuracy over 8 anatomical landmarks showed an overall mean of 0.56±0.16 mm. The developed system was applied to a surgery for a vertical alveolar bone augmentation in right mandibular posterior area and possible interior alveolar nerve injury case of an impacted third molar. The developed system provided continuous monitoring of invisible anatomical structures during operation and 3D information for operation sites. The clinical challenge showed sufficient accuracy and availability of anatomically complex operation sites. Conclusion : The developed system showed sufficient accuracy and availability in oral and maxillofacial surgeries for anatomically complex sites. (Korean J Oral Maxillofac Radiol 2009; 39 : 149-56)

Duplication 9p Syndrome 1례

이미경,노영래,김영훈,백청순,신미경,이진,장필상,김진택,이권행,황경태 대한소아과학회 2002 小兒科 Vol.45 No.9

형상기억복합재료에 대한 온라인 모니터링 시스템 개발

이동화,박영철,이민래,이동화,이규창 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.1

형상기억복합재료를 제조하기 위하여 핫프레스 방법을 이용하여 최적의 제조조건을 도출하였으며 냉간압연에 의해 기지재와 강화재의 계면접합을 강화하여 강도를 증가시켰다. 이러한 방법에 의해 제조된 형상기억복합재료에 대하여 외부 하중에 의한 손상정도와 균열의 발생을 감지하여 균열 발생 및 진전을 억제하는 온라인 모니터링 시스템을 개발하고자 한다. 이를 위하여 음향방출 신호의 파라미터를 이용하여 손상에 따른 최적의 AE 파라미터를 도출하였으며 가열시스템을 이용하여 형상기억합금을 가열함으로써 형상기억합금의 수축에 의한 복합재료 내부에 균열진전을 억제시키는 시스템을 개발하였다. A hot press method was use for the optimal manufacturing condition for a shape memory alloy(SMA) composite. The bonding between the matrix and the reinforcement within the SMA composite by the hot press mothod was strengthened by cold rolling. In this study, the objective was to develop an on-line monitoring system for the prevention of the crack initiation and propagation by shape memory effect of SMA composite. Shape memory effect was used to prevent the SMA composite from cracking. For the system to be developed, an optimal AE parameter should be determined based on the degree of damage and crack initiation. When the SMA composite was heated by the plate heater attached at the composite, the propagating cracks appeared to be controlled by the compressive force of SMA.

ISD 모델에 기초한 농업인 안전교육 프로그램 개발

김진모,이택선,이경숙,김경란,길대환,김효철,이찬 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.3

이 연구의 목적은 ISD 모델에 기초하여 농업인 안전교육 프로그램을 개발하는데 있으며,이를 위해 분석-설계-개발-실행-평가의 다섯 단계로 구성된 개발모형을 설정하였다. 구체적인 프로그램 내용을 개발하기 위해 환경분석,학습자분석,과제분석을 실시하였고,그 결과를 반영하여 ‘안전의식 함양’,‘근골격계질환’,‘농약중독예방’,‘농기계 안전 사용’의 4개 모듈과 10개의 레슨을 설계하였다. 요구분석 및 설계 결과를 바탕으로 농업인 안전교육 프로그램의 교재/보조 자료/매체를 개발하였다. 교재의 경우,학습자용과 강사용을 구분하여 개발하였다. 개발된 프로그램 및 운영체제에 대한 검토 및 보완을 위한 전문가 협의회를 개최하여 프로그램 시연회를 실시한 후 교육프로그램을 수정,보완하였다. 그 후,농작업안전시범마을의 농업인을 대상으로 농업인 안전교육 프로그램을 시험 운영하였고,그 결과를 반영하여 농업인 안전교육 프로그램을 완성하였다. 또한 이상의 프로그램 개발 과정을 통해 나타난 논점을 토대로 향후 교육 프로그램 개발을 위한 시사점을 제공하였다. The purpose of this study was to develop a agricultural safety and health training program based on ISD Model. We set up 5step(Analysis, Design, Development, Implementation, Evaluation) model for developing the program. After analyzing environment, learner and task, four modules(① Safety and Health Mindset, ② Musculoskeletal System Disorders, ③ Agrichemical Poisoning Prevention, and ③ Using Farm Machines Safely) and 10 lessons were designed. We developed teaching materials and supplement materials for the Agricultural Safety and Health Training Program based on need analysis and program design. Teaching materials consisted of the learner's and instructor's. The developed program was examined by expert groups and the weak points were revised through professional workshop. Finally, we tested the Agricultural Safety and Health Training Program at agricultural safety and health model village and fixed the program by reflecting the test results.

A Study on the appropriate herbal medicine according to Sasang constitution using EAV

Choi,Jin-Wook,Lee,Su-kyung,Hong,Suck-chull,Koh,Byung-hee INSTITUTE OF ORIENTAL MEDICINE KYUNG-HEE UNIVERSIT 1997 JOURNAL OF ORIENTAL MEDICINE Vol.2 No.1

1. Objective This is a basic study about the response of herbal medicine to EAV. The purpose was to investigate how the meridian of EAV responded to herbal medicine and how EAV was applied to the diagnosis of Sasang constitution. 2. Materials & Methods The subjects of this study were patients who were being treated in the Dept. of Constitutional Medicine ot Kyung-Hee Medical Center. The study examined how herbal medicine affected the maximum value and the indicator drop and how each constitution responded differently to the constitutional herb medicine. 3. Results & Conclusions 1) When comparing the maximum value and ID of the worst BAP before and after the experiment with herbal medicine, the maximum value significantly went up and the ID significantly went down in all the herbal medicines. So we confirmed that all the herbal medicines positively affected the change of the maximum value and ID. 2) The changes in the maximum value induced by herbal medicines were significantly different according to the constitution of Dokhual Jihwang Tang, and Hyangbuja Palmul Tang. Although Dokhual Jihwang Tang was soyangin's herb medicine in Sasang constitutional medicine, it raised the value of soeumin higher than that of soyangin. Hyangbuja Palmul Tang which was soeumin's herb medicine raised the value of soeumin higher than that of the other constitution. 3) The changes of ID were not significantly different in the constitution.

사상체질과 신체형태지수와의 연관성

박경아,김희진,허경석,안수경,이성호,이종은,이원택 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4