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白鼠舌背粘膜의 蛋白合成와 移動에 關한 自記放射法的 硏究
金孝喆 최신의학사 1974 最新醫學 Vol.17 No.6
Forty-five male mice weighing approximately 20g were used in this study. They received a single intraperitoneal injection of 3pc/g of body weight of phenylalanine -3H (specific activity 3 c/mM) obtained from New England Nuclear Corporation. Mice receiving phenylalanine 'H were sacrificed at 1 hour, 4 hours, 1,4.5.7,9,11 and 14 days after injection. Dorsal tongue of all animals were immediately removed and fixed in 2% paraformaldehyde in cacodylate buffer, sectioned at 4p and stained with toluidine blue O. The slides are dipped into Kodak NTB3 liquid emulsion and exposured for two weeks. Following the exposure, the slides are developed for 1 minutes at 18ºC in a developer and fixed in kodak rapid fixer. The present investigation was undertaken primarily to determine more precisely the turnover time of dorsal lingual mucosa by autoradiographic analysis. The results were as follows. 1. Most cells in the stratum basale of the dorsal lingual. epithelium were radioactived at one day after injection. 2. Phenylalanine labeled cells were reached the lower half portion of the stratum spinosum in 4 days after injection. 3. Seven days after injection radioactive cells had moved up the upper half of stratum s0losum. 4. Tritiated phenylalanine was immediately incorporated into all differentiating epithelial layers, entering the stratum corneum in 9 days after injection, and all labelling was gone by day 14. 5. These and similar observations may be of value in future studies of pathological processes involving
김효철,Kim, Hyo-Cheol 한국과학기술단체총연합회 1995 과학과 기술 Vol.28 No.12
1952년 동란중 부산서 탄생한 대한조선학회는 60년부터 본격적인 활동을 개시. 70년 세계조선시장에서 우리나라가 수주량 제1위를 확보할 수 있는 바탕을 마련하는 역할을 해왔다. 회원 1천 1백72명으로 56개의 단체회원사의 후원을 받아 매년 2차례의 연구발표회를 통한 왕성한 연구활동과 14권의 정기간행물을 발행하고 있으며 97년에는 창립 45주년을 기념해 「조선용어사전」을 발간할 계획이다.