한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.

안면신경마비에 대한 안륜근 미소안면침 증례보고

조아름,김지현,주경옥,원재선,김창환,Cho, Ah-Reum,Kim, Ji-Hyun,Chou, Ching-Yu,Won, Jae-Sun,Kim, Chang-Hwan 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.2

Objective : This study was designed to evaluate the effect of Miso Facial Acupuncture on Orbicularis Oculi muscle. Methods : Two men and four women with Facial nerve palsy were recruited. Miso Facial Acupuncture on Orbicularis Oculi muscle was performed two times a week for three weeks. The effects of the treatment were evaluated by H-B scale, Y-system and clinical symptom. Results : Before treatment with Miso Facial Acupuncture, three patients showed H-B scale improvement and the other three patients had no change. and four patients showed Y-system improvement and the other two patients had no change. In six cases, the patients had improvement in clinical symptom related with Orbicularis Oculi muscle function. Conclusion : Miso Facial Acupuncture on Orbicularis Oculi muscle showed significant improvement in facial nerve palsy.

Association of Change in Smoking Status and Subsequent Weight Change with Risk of Nonalcoholic Fatty Liver Disease

Jeong Seogsong,Oh Yun Hwan,Choi Seulggie,Chang Jooyoung,Kim Sung Min,Park Sun Jae,Cho Yoosun,Son Joung Sik,Lee Gyeongsil,Park Sang Min 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.1

Background/Aims: Smoking is considered a risk factor for the development of nonalcoholic fatty liver disease (NAFLD). However, the association of a weight change after a change in smoking status and the risk of NAFLD remains undetermined. Methods: This study used the Korean National Health Insurance Service-National Sample Cohort. Based on the first (2009 to 2010) and second (2011 to 2012) health examination periods, 139,180 adults aged at least 40 years were divided into nonsmoking, smoking cessation, smoking relapse, and sustained smoking groups. NAFLD was operationally defined using the fatty liver index. The adjusted odds ratio (aOR) and 95% confidence interval (CI) were calculated using multivariable-adjusted logistic regression. Results: Compared to nonsmoking with no body mass index (BMI) change, the risk of NAFLD was significantly increased among subjects with BMI gain and nonsmoking (aOR, 4.07; 95% CI, 3.77 to 4.39), smoking cessation (aOR, 5.52; 95% CI, 4.12 to 7.40), smoking relapse (aOR, 7.51; 95% CI, 4.81 to 11.72), and sustained smoking (aOR, 6.65; 95% CI, 5.33 to 8.29), whereas the risk of NAFLD was reduced among participants with BMI loss in all smoking status groups. In addition, smoking cessation (aOR, 1.76; 95% CI, 1.35 to 2.29) and sustained smoking (aOR, 1.64; 95% CI, 1.39 to 1.94) were associated with higher risk of NAFLD among participants with no BMI change. The liver enzyme levels were higher among participants with smoking cessation and BMI gain. Conclusions: Monitoring and management of weight change after a change in smoking status may be a promising approach to reducing NAFLD.

이상기후 발생이 가을배추 생산자 및 소비자에게 미치는 영향

조재환 ( Jae Hwan Cho ),서정민 ( Jeong Min Suh ),강점순 ( Jum Soon Kang ),홍창오 ( Chang Oh Hong ),신현무 ( Hyun Moo Shin ),이상규 ( Sang Gyu Lee ),임우택 ( Woo Taik Lim ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.12

The purpose of this article is analyzing the economic impacts of abnormal climate on fall chinese cabbage farmers and consumers in Korea, with employing the equilibrium displacement model. Our results show that there were little difference in gross farm income, even though there were significant yield reductions due to abnormal climate changes. However periodic occurrences of abnormal climates caused serious damage to consumption levels which had declined by 10.6~17.1 percent with higher prices by 15.3~24.6 percent than normal climate years since 1990.

Lipids Induce Release of Tumor-Promoting Exosomes from Hepatocellular Carcinoma Cells

( Eun Ju Cho ),( Hyo Yeong Lee ),( Joon Yeul Nam ),( Young Chang ),( Hyeki Cho ),( Young Youn Cho ),( Jeong-hoon Lee ),( Su Jong Yu ),( Yoon Jun Kim ),( Jung-hwan Yoon ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Hypoxia enhances lipid droplets accumulation by HIF-dependent mechanism, leading to intra-tumoral fatty metamorphosis which is one of the characteristics of early-stage hepatocellular carcinoma (HCC). We investigated whether lipids and lipids-induced exosomes released from HCC cells can induce a tumor-promoting phenotype. Methods: Human HCC cell lines (Huh-7, SNU-761, and SNU-3058) were incubated with oleic acid (OA) or control vehicle. The released exosomes were isolated, quantified, and applied to HCC cells. Results: Incubation of Huh-7 and SNU-761 cells with OA increased proliferation and migration of cells in a fatty-acid binding protein 3 (FABP-3) dependent manner, whereas SNU-3058 cells did not respond to OA. Furthermore, OA upregulated FABP-3 mRNA expression in Huh-7 and SNU-761 cells, whereas its expression in SNU-3058 cells did not change. OA enhanced release of exosomes from Huh-7 and SNU-761 cells, and exosomes collected form these cells upregulated proliferation and invasion of cells. However, this was not observed in SNU-3058 cells. Exosomes released from OA-treated cells were reduced by the inhibition of rho-associated, coiled-coil-containing protein kinase 1 (ROCK1) or FABP-3. Conclusions: These findings suggest that lipids induce release of exo-somes containing unique cargoes from HCC cells in a FABP-3 dependent manner, which may induce the progression of HCC.

기후변화와 키메라현상에 대한 계층 간의 관계인식

백정애(Jungae Baik),김인경(In Kyoung Kim),김진영(Jin Yung Kim),노용환(Yong Hwan Noh),배선영(Sunyoung Bae),우명원(Myoung Won Woo),장혁기(Hyukki Chang),조성돈(Sung Don Cho),장매희(Maehee Chiang) 인간식물환경학회 2011 인간식물환경학회지 Vol.14 No.3

This study was investigated to research the basis of interdisciplinary fusion through the survey on the basis of the answers of professionals and general public, gender, humanities, science and engineering about the question of climate change and chimera which is one of the various phenomena that affect the human life. All respondents’ perceptions of climate change was high and natural disasters was showed the most destruction according to climate change. The answer of awareness of climate change and the damage, chimerism was significant differences between age groups sex, specialized domain-specific individuals. In addition, a group of respondents for science and engineering professionals was higher for awareness of climate change and the chimerism and heir negative opinion for chimerism was relatively low in comparison of other research groups.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

고관절의 절제 관절성형술

유명철 ( Myung Chul Yoo ),조윤제 ( Yoon Je Cho ),김용환 ( Yong Hwan Kim ),조창현 ( Chang Hyun Cho ),박병주 ( Byung Joo Park ),조영린 ( Young Lin Cho ) 대한고관절학회 1997 Hip and Pelvis Vol.9 No.2

Girdlestone operation is a resection arthroplasty, which is composed of resection of head and neck of femur and construction of pseudoarthrosis, which was described by Girdlestone at first in 1928. The purpose of this study was to establish the efficacy of Girdlestone operation. In 4I hips of 40 patients, who had undergone Girdlestone operation and was not converted to total hip replacement from Dec. 1986 to Sep. 1996, were reviewed. Average follow up period was 3 years and 10 months(range from one year to ten years and ten months). Twenty four patients (25cases) were males and sixteen patients were females. The average age was 49 years old(range from 19 to 77 years old). Preoperative diagnoses were infection in 32 cases, posttraumatic osteoarthritis in 3 cases, pelvic bone fracture in 2 cases, ankylosing spondylitis involving hip joint in 2 cases(1 patient), RDC(rapidly destructive coxarthrosis) in one case and femoral neck fracture in one case. Pain was relieved completely or decreased markedly in all cases after Girdlestone operation. Preoperatively, four patient(five cases) had not been able to walk and thirty six patients had showed the moderate or severe limping. After operation, thirty cases showed the moderate 1imping. In the analysis of walking grade, preoperatively, four patients(five cases) had not been able to walk and 36 cases had used cane or crutch, postoperatively, 16 cases walked without any support, 9 cases used one cane, and 16 cases used two crutch. The range of motion of the hip joints was improved postoperatively. Preoperative flexion contracture, average fourteen degrees decreased to average three degrees postoperatively. Preoperative further flexion, average sixty eight degrees, increased to ninety degrees postoperatively. The average shortening of operated limb was 4.5 cm. Average time interval from operation to crutch ambulation was 37 days. In the assessment of patient satisfaction, most patients were satisfied with the improvement of pain, physical activity and psychosocial activity except morphological change. Our opinion is that Girdlestone operation is relatively an effective and safe procedure to treat the infected or dysfunctioned hips.

종 분포 모형을 활용한 새로운 구상나무 서식지 탐색, 그리고 식물보전 활용

김남신 ( Nam Shin Kim ),한동욱 ( Dong Uk Han ),차진열 ( Jin Yeol Cha ),박용수 ( Yong Su Park ),조현제 ( Hyeun Je Cho ),권혜진 ( Hye Jin Kwon ),조용찬 ( Yong Chan Cho ),오승환 ( Seung Hwan Oh ),이창석 ( Chang Seok Lee ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2015 한국환경복원기술학회지 Vol.18 No.6

Korean fir(Abies koreana E.H.Wilson 1920), endemic tree species of Korean peninsula, is considered as vulnerable and endangered species to recent rapid environmental changes such as land use and climate change. There are limited activities and efforts to find natural habitats of Korean fir for conservation of the species and habitats. In this study, by applying SDMs (Species Distribution Models) based on climate and topographic factors of Korean fir, we developed Korean fir’s predicted distribution model and explored novel natural habitats. In Mt. Shinbulsan, Youngnam region and Mt. Songnisan, we could find korean fir’s two novel habitat and the former was the warmest(13°C in annual mean temperature), the driest(1,200mm∼1,600mm in annual rainfall) and relatively low altitude environment among Korean fir’s habitats in Korea. The result of SDMs did not include mountain areas of Gangwon-do as habitats of A. nephrolepis, because there were different contributions of key habitat environment factors, summer rainfall, winter mean temperature and winter rainfall, between A. koreana and A. nephrolepis. Our results raise modification of other distribution models on Korean fir. Novel habitat of Korean fir in Mt. Shinbulsan revealed similar habitat affinity of the species, ridgy and rocky site, with other habitats in Korea. Our results also suggest potential areas for creation of Korea fir’s alternative habitats through species reintroduction in landscape and ecosystem level.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.