鼻症狀을 동반한 Maxillary Sinus Retention Cyst 1예

한은정,김윤범,Han, Eun-jeong,Kim, Yoon-bum 대한한방안이비인후피부과학회 2003 한방안이비인후피부과학회지 Vol.16 No.1

Maxillary sinus retention cysts are probably common findings on PNS series. Many patients remain asymptomatic in the presence of these lesions, but a variety of local and systemic symptoms have been associated with them. A patient presented with symptoms of chronic rhinosinusitis had a finding of both maxillary sinus retention cysts on PNS series. He has taken the Gal-gen-tang and treated by acupunture & infra-red. After about 4 months' treatment, his symptoms resolved and PNS series were normal. In conclusion, Maxillary sinus retention cysts may become clinically important when they occur in the setting of symptoms compatible with chronic rhinosinusitis, or when the diagnosis is in doubt.

갈근탕(葛根湯)이 면역조절작용에 미치는 영향

조대연,윤용갑,정명,이은혜,복영옥,정창옥,임규상,Cho, Dae-Yeoun,Yun, Young-Gab,Jung, Myung,Lee, Eun-Hye,Bok, Young-Ok,Jung, Chang-Ohk,Lim, Kyu-Sang 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : Gal-Geun-Tang (GT) has been described from SANGHAN in Korean traditional medicine and known to act against cold, fever, hypertension, and nasal catarrh. However, little has yet been learned about the effect of GT on immune function. In the current study, in vitro and in vivo immunomodulatory activity of GT (water extract) was investigated.Methods : Water extract of GT induced in vitro proliferation of spleen cells and significantly increased their proliferative responses during anti-CD3 activation. Using purified splenic T and B cells, it was revealed that GT has a mitogenic activity to B cells and promotes their proliferation induced by lipopolysaccharide, whereas T cell proliferation was not triggered and GT was rather inhibitory to T cell activation caused by anti-CD3 antibody. In the presence of antigen presenting cells (APC), GT addition resulted in a significant increase of IFNγ and IL-4, but not IL-2, production. However, addition of high concentration (1,000㎍/㎖) of GT led to a marked reduction in T cell cytokine production and under such condition, GT facilitated apoptosis of T cells when examined by flow cytometry with propidium iodide staining.Results : In vivo immunomdulation of GT was also investigated using a mouse model. Following keyhole limpet hemocyanin (KLH) immunization, GT (1 ㎎/day) was orally administered for 9 days. Cell numbers in thymus, spleen and peripheral blood were not altered by GT administration, indicating that such dose is not immunotoxic. Cell numbers in draining lymph nodes (LN) and ex vivo Ag-specific proliferation of LN cells were significantly elevated by GT administration. However, any preferential stimulation of T or B and CD4⁺ or CD8⁺ T cell subpopulations was not observed in a flow cytometric analysis of LN cells. This result shows that GT does not promote in vivo B cell proliferation while GT enhances Ag-specific proliferation of LN cells, unlike what was observed in vitro.Conclusions : For a further understanding of in vivo immunomodulatory activity of GT, ex vivo cytokine production of LN cells obtained from KLH-immunized mice was evaluated. Ag-specific IFNγ production was significantly higher in GT-treated mice when compared to PBS-treated control mice. In contrast, IL-4 production in GT-treated group was comparable to control group unlike to in vitro data. In addition, GT administration did not result in any significant differences in serum levels of Ig (IgM, IgG1 and IgG2a) between GT-treated and control groups. Taken together, these data strongly support that GT promotes immune response, more profoundly type 1 helper T cell (Th1) activity and GT may be applicable for treatment of intracellular parasite infection such as viral diseases.

패혈증 쇼크에서 단삼 추출물의 억제 효과와 그 기전에 관한 연구

신상호,윤정민,고우신,윤화정,Shin, Sang-Ho,Yun, Jeong-Min,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : This study was performed to investigate the protective effects and mechanisms of Salvia miltiorrhizae Radix extract (SME) on endotoxin shock.Methods : We used two models; LPS-induced sepsis model for in vivo model, and murine peritoneal macrophages responses for in vitro. SME was administrated orally to mice. After 1 hr, LPS was injected intraperitoneally. Survival rate was checked each time per 12 hr for 5 days. Mice were sacrificed 3 hr after LPS injection, then blood samples and organs were harvested. Cytokines secretion was measured by ELISA. Organs tissues were observed with microscope. Murine peritoneal macrophages were cultured for 1 hr either in a medium alone or in a medium that contained SME, as indicated. Then, the cells were treated with LPS for 24 hr. mRNA levels of cytokines were measured by real-time RT-PCR. Cytokine levels in the supernatants were measured by ELISA. The amount of nitrite was measured by using the Griess method to evaluate NO production. The cell lysates were analysed by Western blotting using antibodies for iNOS and β-actin was used as an internal control to monitor equal protein loading.Results : SME improverd the survival rate of mice model. SME inhibited the secretion of inflammatory cytokines and organs damages on Endotoxin Shock model. SME suppressed cytokine expression, cytokine secretion,NO production, iNOS expression in LPS-induced murine peritoneal macrophages.Conclusions : The results suggest that SME has protective effects on endotoxin shock through suppression of inflammatory cytokines, organ damages, NO production and so on.

건칠의 항주름 효과와 미백효과 연구

안재형,김경준,Ahn, Jae-Hyung,Kim, Gyung-Jun 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro.Methods : Human dermis cell (HS68) was used to test the effect of Rhus verniciflua Stokes (RVS). 80% ethanol or water extracts were screened for their inhibitory activities against elastase. Elastase inhibition effect was tested by microplate reader instrument. And MMP-1 suppression effect of RVS was tested by western blot. These cells were investigated the viability by MTS assay. And also the inhibition effect of tyrosinase by RVS was tested.Results : RVS (final concentrstion 1 ㎎/㎖ appeared over 30% of inhibition of elastase activity. So we are investigated anti wrinkle effects of Rhus verniciflua Stokes look through MMP-1 inhibition activity, also Extracts of RVS showed higher anti-tyrosinase activity than arbutin as final concentration 1 mg/ml. These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.Colclusions : RVS was the best suppressor candidate of elastase activity among other control oriental drugs. It was found that RVS did not have toxicity to cells. We found proper concentration of RVS to treat in HS68 culture and investigated not only wrinkle care effect but also whitening effect of RVS.

계지작약지모탕(桂枝芍藥知母湯)이 Acetic Acid에 의한 통증에 미치는 영향

노희엽,김연섭,김도훈,Noh, Hee-Youb,Kim, Youn-Sub,Kim, Do-Hoon 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : We want to know the effect of Gyejakjimo-tang in mice model of acute pain.Methods : We investigated writhing reflex in mice with acetic acid-induced abdominal pain using mice, we observed c-Fos protien expression by immunohistochemistry dyeing method in the paraventricular nucleus(PVN) and supraoptic nucleus(SON) of the hypothalamus.Results : All of Gyejakjimo-tang treated group suppressed acetic acid-induced writhing response as acetic acid injuction group, but in 100㎎/㎏ Gyejakjimo-tang treated group and 200㎎/㎏ Gyejakjimo-tang treated groups represernted significance. All of Gyejakjimo-tang treated groups(50, 100 and 200㎎/㎏ Gyejakjimo-tang-treated group), Fos-positive cells in PNV significantly decreased as acetic acid injuction group, and All of Gyejakjimo-tang-treated groups Fos-positive cells in SON significantly decreased as acetic acid injuction group.Conclusions : The present results showed that the mice pre-treated with the aqueous extract of Gyejakjimo-tang showed analgesic effect on acetic acid-induced abdominal pain.

광역동요법을 병행한 두피 모낭염 한방치험 1례

안진향,윤영희,고성규,최인화,Ahn, Jin-Hyang,Yun, Young-Hee,Ko, Seong-Gyu,Choi, In-Hwa 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : The purpose of this study is to report the effect of combination therapy of photodynamic therapy (PDT) with Korean medical treatments on scalp folliculitis, which has been difficult to treat by using antibiotic.Methods : A 32-year-old male patient with scalp folliculitis, received treatment with acupuncture, herbal medicine and PDT and so on. We used Visual Anologue Scale(VAS) to evaluate recovery from erythema, pruritus and pain. Also, the photos of patient were taken before and after treatments.Results : Visible symptoms to the naked eye and VAS scale effectively decreased after Korean medicine treatments for two months.Conclusions : It is expected that Korean medical treatments and PDT were thouht to be effective for scalp folliculitis.

특발성 양안성 핵간안근마비 1례

엄예진,홍철희,Eom, Ye-Jin,Hong, Chul-Hee 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : The aim of this study is to report the effect of Korean medicine treatment on bilateral internuclear ophthalmoplegia without abnormal findings on brain magnetic resonance imaging.Methods : The patient was treated by using acupuncture, electroacupuncture treatment and hominis placenta pharmacoacupuncture. The change of eye movement and strabismus were evaluated by comparison the photographs of the extraocular movements of patient. Strabismus also was evaluated by corneal reflex test.Results : Adduction of both eyes were improved after Korean medicine treatment. The amplitude and frequency of nystagmus showed a tendency to decrease in both eyes.Conclusions : Korean medicine treatment should be effective for the idiopathic bilateral internuclear ophthalmoplegia.

행인(杏仁)이 전립선 암세포의 Bax, Bcl-2 및 Caspase-3에 미치는 영향

이도경,김연섭,김도훈,Lee, Do-Kyung,Kim, Youn-Sub,Kim, Do-Hoon 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Prostate cancer is one of the most common non-skin cancers in men. Armeniacae Amarum Semen has traditionally been used for the treatment of inflammation diseases, leprosy, leucoderma, and tumors. Apoptosis, which is also known as programmed cell death, is an important mechanism in cancer treatment.Objectives : We observed whether an aqueous extract of Armeniacae Amarum Semen induces apoptotic cell death in human DU145 prostate cancer cells.Methods : We treated DU145 cells with Armeniacae Amarum Semen extract and investigated characteristics of apoptosis. And investigated whether treated with Armeniacae Amarum Semen extract increased Bax mRNA expression, Bcl-2 mRNA expression, caspase-3 enzyme activity and their protein level.Results : We have shown that Armeniacae Amarum Semen extract can induce apoptotic cell death in human DU145 prostate cancer cells by caspase-3 activation through the down-regulation on Bcl-2 expression and the up-regulation on Bax expression.Conclusions : It can be expected that an aqueous extract of Armeniacae Amarum Semen may offer a valuable means for the treatment of prostate cancers.

문헌적 근거를 기반으로 한 아토피 피부염의 소화기계·호흡기계로의 변증분류

박승구,노현민,황충연,홍석훈,박민철,장현철,김호경,Park, Sung-Gu,Noh, Hyeon-Min,Hwang, Chung-Yeon,Hong, Seok-Hoon,Park, Min-Cheol,Jang, Hyun-Chul,Kim, Ho-Kyoung 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives: This study aimed to verify the classification of atopic dermatitis into the digestive and respiratory disorder on the basis of a literature study.Methods: We searched for the term "atopic dermatitis" in KISS, RISS, NDSL, DBPIA, and OASIS. On further filtering the searched paper further by including the terms "Korean medicine", "literature study" and excluding "acupuncture", we found eight papers relevant to the literature study of atopic dermatitis. The reviewed papers included keywords of Oriental medicine such as Sub-yeol (濕熱), Bi-heo (脾虛), Poong-sub (風濕), Hyeol-heo (血虛). We classified keywords as "digestive system" or "respiratory system" and compared the clinical symptoms of classified Each category keywords.Results: Atopic dermatitis was classified into four categories : acute digestive disorder, chronic digestive disorder, acute respiratory disorder, and chronic respiratory disorder. The four categories of atopic dermatitis showed differences based on region, dermal symptoms, associated symptoms, and affected age group.Conclusions and Discussions: This study suggests a new theory of Atopic dermatitis classification. The theory is similar to that stated in former classification; however, it focuses on the digestive and respiratory disroder. All of atopic dermatitis into digestive disorder show both digestive symptoms and dermal symptoms. However, atopic dermatitis into respiratory disorder shows only respiratory symptoms. Thus, this study can establish a relationship between Western and Oriental medicine's study of atopic dermatitis using keywords such as "digestive disorder" and "respiratory disorder".

아토피피부염 개인건강기록 어플리케이션 개발을 위한 전문가 집단 설문조사 연구

김영은,김안나,이동효,박민철,손미주,장현철,Kim, Young-Eun,Kim, An-Na,Lee, Dong-Hyo,Park, Min-Cheol,Son, Mi Ju,Jang, Hyun-Chul 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : We conducted a survey aimed at developing a personal health record application for the treatment of atopic dermatitis in Korean medicine .Methods : We conducted a survey on Korean medicine doctors who attended the Korean Medicine Ophthalmology and Otolaryngology and Dermatology conference 2016. The questionnaire was based on priority of usage of the diagnostic indices and tools, and intention to use the personal health record application for treatment of atopic dermatitis in the clinic.Results : Data were collected from 50 Korean medicine doctors. Ninety-six per cent of respondents replied that they were willing to use the personal health record application for treating atopic dermatitis. Among the diagnostic indices related to atopic dermatitis, Korean medicine doctors regarded the following as important in the order of priority, i.e., condition of skin, lifestyle, risk factors, symptoms other than those of skin, past history, family history and medical history, results of tongue, pulse, and abdomen investigation, and constitution. These results did not vary with the purpose of diagnosis, and the results were consistent with those obtained with the intention to use diagnostic. Over 50% of respondents replied that they use immunoglobulin E, scoring atopic dermatitis, and visual analogue scale among the diagnostic tools.Conclusions : Our survey was conducted on clinicians who are the intended users of the personal health record application for the treatment of atopic dermatitis; hence, the results of this study can be helpful for developing a useful personal health record application for atopic dermatitis in the clinic.