살균제 Pyraclostrobin과 대사체 BF500-3의 순무유채 중 잔류특성

임다정,김영은,김선욱,윤지현,김인선 한국농약과학회 2019 한국농약과학회 학술발표대회 논문집 Vol.2019 No.10

살균제 Boscalid의 갓 중 잔류특성

임다정,김영은,김선욱,윤지현,김인선 한국농약과학회 2019 한국농약과학회 학술발표대회 논문집 Vol.2019 No.10

Biosurfactant 기반 Hybrid형 병해충 방제제 개발에 대한 연구사례 소개

임다정,김인선 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

Identification of lipopeptide xantholysins from Pseudomonas sp. DJ15 and their insecticidal activity against Myzus persicae

임다정,양시영,노미영,이철원,김진철,김인선 한국곤충학회 2017 Entomological Research Vol.47 No.6

Myzus persicae is an important insect pest that reduces crop production worldwide. The use of pesticides for aphid control has generated much concern related to insect resistance and undesirable environmental effects. In an effort to discover new alternatives to counter M. persicae, we found that Pseudomonas isolate DJ15 produced insecticidal metabolites. To isolate the insecticidal metabolites, a cell‐free supernatant of DJ15 was extracted and subjected to bioassay‐guided chromatography. Based on the structures elucidated in instrumental analyses, the metabolites were identified as xantholysins A and B. The metabolites showed strong insecticidal activity against M. persicae with 50% mortality at levels of 13.4 and 24.6 μg/mL for xantholysins A and B, respectively. This is the first study to identify xantholysins as insecticidal metabolites against M. persicae.

Attraction of the Invasive Hornet, Vespa velutina nigrithorax, by using Bacillus sp. BV‐1 Cultures

임다정,이정은,이진실,김익수,김인선 한국환경농학회 2019 한국환경농학회지 Vol.38 No.2

BACKGROUND: The invasive hornet Vespa velutina nigrithorax has becomes a public concern in rural and urban South Korea. The technologies are necessary to develop a way to counter V. velutina. In an effort to develop a way to counter V. velutina, we found that a bacillus strain, named Bacillus sp. BV‐1, produces volatile compounds that attract V. velutina. METHODS AND RESULTS: Field trials of V. velutina attraction were performed using plates and traps containing BV‐1 cultures grown on sugar medium. When the sugar medium and sugar‐grown BV‐1 cultures in the plates were placed close together, V. velutina visited preferably the plates with the BV‐1 cultures. Significantly more V. velutina were caught in the traps containing BV‐1 cultures than in those containing only sugar medium. Headspace solid‐phase microextraction coupled with GC/MS analysis of BV‐1 cultures detected 2‐methyl‐1‐propanol, 3‐methyl‐1‐butanol, 3‐methylbutanoic acid, ethyl hexanoate, 2‐pheylethanol, ethyl octanoate, and ethyl decanoate as the major volatiles. CONCLUSION: BV‐1 cultures were suggested as potential agents for managing V. velutina as they produce volatile compounds that attract the hornet.

Fabrication and Properties of Glass Fiber-Reinforced Composites Using Polyimide Prepregs with Inorganic Nanofillers

임다정,박준철,장시훈,최준,박노형 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3

Numerous studies have addressed the utilization of polyimide (PI) and silica for improving the properties of hybridcomposites. However, the applicability of polyhedral oligomeric silsesquioxanes (POSSs) has not been elucidated. Herein,inorganic nanoparticles containing poly(amic acid)s (PAAs) were synthesized; further, glass fibers (GFs) were impregnatedwith PAA solutions to obtain PAA prepregs. PI-based fiber-reinforced composites were manufactured with the prepregs byusing the hand lay-up technique. The morphology and the thermal, mechanical, and dielectric properties of the compositeswith inorganic nanoparticles were analyzed to investigate the effects of inorganic additives. The addition of inorganicparticles were observed to primarily improve the properties of the PI-based fiber-reinforced composites as compared to thoseof the neat PI composites. While this improvement was limited in the case of the addition of POSSs owing to the aggregationand a poor interaction with the PI matrix, silica-containing composites exhibited superior thermal, mechanical, andparticularly dielectric properties as compared to those of the composites containing POSSs; this was because of the increasein the dispersion of silica nanoparticles that was caused by a silane coupling agent (SCA). The results obtained in this studycan be utilized for applications pertaining to the design of PI composites with high mechanical strengths, enhanced thermalstabilities, and low dielectric constants.

한우와 칡소의 선발신호 분석을 통한 한우의 인위적 선발 영역 탐색

임다정,이승환,최봉환,채한화,손주환,유다영,김태헌,강희설,조용민 경상대학교 농업생명과학연구원 2013 농업생명과학연구 Vol.47 No.6

Korean cattle (Hanwoo) are categorized into three types of breeds according to their color, brown, brindle and black. Among the three breeds, the brown Hanwoo has been subjected to intensive artificial selection over the past seventy years to improve meat production traits such as carcass weight and marbling. In this study, three methods (Rsb, iHS and FST) were applied to identify genomic regions of artificial selection for traits in Hanwoo. To identify traces driven by the artificial selection process called the Hanwoo breeding program, we scanned the genome of set of brown and brindle Hanwoo using 50K SNP Chip. As a result, we identified 41, 23 and 50 highly homozygous regions that seem to be very strong and/or recent artificial selections in brown Hanwoo compared to the brindle Hanwoo from Rsb, iHS and FST, respectively. To go further in the interpretation of the observed signatures of selection, we subsequently performed functional enrichment analysis using candidate genes. As a result, candidate genes have enriched GO terms such as muscle development or immune response. Artificial selection might have targeted most of these genes was mainly oriented towards improvement of meat production in brown Hanwoo. 우리나라 고유 품종인 한우는 표현형 중 외모에 따라 일반적으로 한우라 불리는 황갈색 한우, 칡소, 흑우로 구분되어 있다. 한우 집단 중, 황갈색 한우만이 육질, 육량 형질을 중심으로 체계적인 개량프로그램에 의해 개량되었다. 본 연구에서는 황갈색 한우와 칡소의 50K 고밀도 칩을 활용하여 선발신호를 검출할 수 있는 세 가지 분석법(Rsb, iHS, FST)에 따라 인위적 선발에 의해 진화적으로 선택된 유전체 영역을 탐색하였다. 그 결과, Rsb, iHS, FST 분석법에 따라 각각 41개, 23개, 50개의 후보유전체 영역이 칡소와 비교하였을 때 황갈색 한우에서 유의적으로 다름을 알 수 있었다. 또한, 후보 유전체 영역 내에 존재하는 유전자군을 대상으로 유전자 기능에 대한 주석 달기를 진행하였을 때, 근육 발달, 면역 반응 등의 기능들이 통계적으로 유의함을 알 수 있었다. 이러한 유전체 영역들이 진화적 관점에서 황갈색 한우의 적응과정에 있어서 육질 등 생산성을 높이는 데에 기여하였다고 사료된다.

Rsb 분석법을 활용한 칡소의 선발신호 탐색 연구

임다정,이승환,최봉환,손주환,조용민,채한화,김태헌,강희설 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.4

Korean cattle (Hanwoo) are categorized into three types of breeds according to their color,namely brown, brindle and black. Among the three breeds, Chikso (brindle Hanwoo) have beenmaintained at very low population sizes in South Korea and have unique brindle patterns. Inthis study, Rsb method was applied to identify genomic regions of artificial selection for traitsin Chikso. To identify traces driven by the artificial selection process, we scanned the genomeof set of brown and brindle Hanwoo using 50K SNP Chip. As a result, we identified 37 highlyhomozygous regions that seem to be very strong and/or recent selections in Chikso compared to the brown Hanwoo from Rsb analysis. Among these regions, BTA11:100-102.5 Mb is thestrongest selection signal having 17 significant SNPs. To go further in the interpretation of theobserved signatures of selection, we subsequently performed functional enrichment analysis using207 candidate genes. As a result, candidate genes have enriched GO or KEGG pathway termssuch as unsaturated fatty acids metabolic process(e.g., PTGES2 and ANGPTL2) or immunesystem (e.g., Fc gamma R-mediated phagocytosis). They have important roles in thedomestication process of livestock. We also detected the common signature (BTA1:2 – 2.5 Mb)of selection between Chikso and brown Hanwoo. SYNJ1 gene residing within the region isassociated with the horn development as candidate gene. This observation suggests that Chiksoand brown Hanwoo have a common genetic characteristic as Hanwoo breed duringdomestication. However, only one selection signature means that they seem to be processed in adifferent way during domestication. Chikso may has a founder effect as very low populationfrom the results of heterozygosity and fixed SNPs calculation. From the results, this analysisalso led to the identification of candidate genes as having a strong signal of artificial selectionin Chikso. 우리나라 고유 품종인 한우는 표현형 중 외모에 따라 일반적으로 한우라 불리는 황갈색 한우, 칡소,흑우로 구분되어 있다. 한우 집단 중, 칡소는 한우의 원형으로 여겨지고 있으나, 제주흑우와 비슷하게국내 희소품종으로 분류되어 있다. 현재 본 연구에서는 황갈색 한우와 칡소의 50K 고밀도 칩을 활용하여 선발신호를 검출할 수 있는 Rsb 분석에 따라 인위적 선발에 의해 진화적으로 선택된 유전체 영역을탐색하였다. 그 결과, 37개의 후보유전체 영역이 한우와 비교하였을 때 칡소에서 유의적으로 다름을 알수 있었다. 후보 유전체 영역 내에 존재하는 유전자군을 대상으로 유전자 기능에 대한 주석 달기를 진행하였을 때, 불포화지방산 대사 과정, 면역 반응 등의 기능들이 통계적으로 유의함을 알 수 있었다. 이러한 유전체 영역들이 진화적 관점에서 칡소의 적응과정에 있어서 칡소만의 고유한 유전적 특성을 가지는데 기여하였다고 사료된다. 또한, 황갈색 한우의 선발신호와 칡소의 선발신호를 비교한 결과, 염색체 1번 2-2.5Mb 영역이 진화적 관점에서 공통적으로 선발되고 있음을 관찰할 수 있었으며, 해당 영역의 유전자를 살펴본 결과, 각 발달과정에 관여하는 유전자가 포함되어 있음을 알 수 있었다. 이는 한우라는 공통적 특성을 가지며 진화적으로 유지, 보존하고 있다고 예측할 수 있다. 칡소와 황갈색 한우가가지는 선발신호는 거의 대부분이 다르다는 것을 Rsb 분석에 의해 확인하였다. 추가적으로 집단 내 고정된 단일염기서열변이 및 이형접합율을 비교한 결과 소수 집단에서 가지고 있는 유전적 변이 감소, 유전적 흐름의 급격한 변화 등의 유전적 특성이 칡소 집단에서 나타났으며, 이는 칡소의 선발신호 분석결과와 유사한 결과를 보임을 제시하고 있다. 이러한 결과는 향후 칡소를 비롯한 국내 한우에 대한 연구에 기초자료가 될 것이라 판단되며, 국내 한우 집단 차이의 정확한 원인에 대한 연구는 추가적으로 수행할 필요가 있을 것이라 사료된다.

동물유전체 빅데이터 공개로 민관협업체계 조성 및 산업 활성화

임다정 한국정책학회 2018 한국정책학회 학술대회 Vol.2018 No.-

Fabrication and Mechanical Properties of Glass Fiber Reinforced Polyimide Composites with High Thermal Stability

임다정,박준철,윤슬기,박노형 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.2