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A539 및 NCI-H460 인체 폐암세포의 증식 및 apoptosis 유도에 미치는 가미삼기보폐탕의 영향
김진영,김현중,정광식,박철,최영현,감철우,박동일,Kim, Jin-Young,Kim, Hyun-Joong,Jung, Kwang-Sik,Park, Cheol,Choi, Yung-Hyun,Kam, Cheol-Woo,Park, Dong-Il 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1
Objective : This study was designed to investigate the effect of the water extract of Gamisamgibopae-tang(GMSGBPT), an oriental herbal formulation, on the growth of NCI-H460 and A549 human non-small-cell lung cancer cell lines. Methods : Cytotoxicity and cell morphology were evaluated by MTT assay and inverted microscope, respectively. Apoptosis was detected using agarose gel electrophoresis and flow cytometer. The expression levels of mRNAs and proteins of target genes were determined by RT-PCR and western blot analyses, respectively Result and Conclusion : We found that exposure of A549 cells to GMSGBPT resulted in the growth inhibition in a dose-dependent manner as measured by MTT assay, but GMSGBPTdid not affect the growth of NCI-H460 cells. The anti-proliferative effect of GMSGBPT treatment in A549 cells was associated with morphological changes, formation of apoptotic bodies and DNA fragmentation, and flow cytometry analysis confirmed that GMSGBPT treatment increased the populations of apoptotic-sub G1 phase. Growth inhibition and apoptotic cell death by GMSGBPT were connected with a up-regulation of cyclin-dependent kinase inhibitor p21 (WAF1/CIP1) mRNA and protein in a tumor suppressor p53-independent fashion. However GMSGBPT treatment did not affect other growth regulation-related genes such as early growth response-1 (Egr-1), nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1), inducible nitric oxide synthase (iNOS), cyclooxygenases (COXs), telomere-regulatory factors in A549 orNCI-H460 cells. Taken together, these findings partially provide novel insights into the possible molecular mechanism of the anti-cancer activity of GMSGBPT.
질량스펙트럼의 펩타이드 분자량 오차범위 재해석에 의한 단백질 동정의 성능 향상
권경훈,김진영,박건욱,이정화,백융기,유종신,Gwon, Gyeong-Hun,Kim, Jin-Yeong,Park, Geon-Uk,Lee, Jeong-Hwa,Baek, Yung-Gi,Yu, Jong-Sin 한국생물정보시스템생물학회 2006 Bioinformatics and Biosystems Vol.1 No.2
In proteomics research, proteins are digested into peptides by an enzyme and in mass spectrometer, these peptides break into fragment ions to generate tandem mass spectra. The tandem mass spectral data obtained from the mass spectrometer consists of the molecular weights of the precursor ion and fragment ions. The precursor ion mass of tandem mass spectrum is the first value that is fetched to sort the candidate peptides in the database search. We look far the peptide sequences whose molecular weight matches with precursor ion mass of the mass spectrum. Then, we choose one peptide sequence that shows the best match with fragment ions information. The precursor ion mass of the tandem mass spectrum is compared with that of the digested peptides of protein database within the mass tolerance that is assigned by users according to the mass spectrometer accuracy. In this study, we used reversed sequence database method to analyze the molecular weight distribution of precursor ions of the tandem mass spectra obtained by the FT LTQ mass spectrometer for human plasma sample. By reinterpreting the precursor ion mass distribution, we could compute the experimental accuracy and we suggested a method to improve the protein identification performance. 프로테오믹스에서 얻는 탄뎀 질량 스펙트럼은 효소로 가수분해된 펩타이드의 전구이온(precursor ion) 분자량과 펩타이드에 에너지를 가하여 생성된 이온조각(fragment ion)들의 분자량값들로 구성된다. 탄뎀 질량스펙트럼의 전구이온 분자량은 단백질 서열 데이터베이스에서의 검객 과정에서 가장 먼저 고려하는 값이다. 단백질 검색 프로그램은 단백질 서열 중에 스펙트럼의 전구이온으로부터 계산된 분자량과 일치하는 펩타이드 서열들을 찾아내고, 이들 중의 하나를 이온조각들의 분자량 정보를 이용해서 선택한다. 이 때에 전구이온의 분자량은 사용자가 지정한 오차범위 내에서 일치하는 감을 검색하는데, 이때의 오차범위는 질량분석기의 정확도에 따라 결정된다. 본 논문에서는 인간 혈액의 혈장시료로부터 FT LTQ 질량분석기를 통해 얻어진 탄뎀 질량 스펙트럼에서 전구이온 분자량의 분포를 역순서열을 이용하여 분석하였다. 전구이온 분자량의 분포를 재해석하여 실험값의 정확도를 보정하고 단백질 동정의 성능을 향상시키는 방법을 모색하였다.
α+β 타이타늄 합금의 미세조직 제어에 따른 기계적 특성
황유진 ( Yu-jin Hwang ),박양균 ( Yang-kyun Park ),김창림 ( Chang-lim Kim ),김진영 ( Jin-yung Kim ),이동근 ( Dong-geun Lee ) 한국열처리공학회 2016 熱處理工學會誌 Vol.29 No.5
The mechanical properties of Ti-6Al-4V can be improved by microstructural control through the heat treatment in α+β region. The heat treatment was carried out with a variety of heat treatment temperatures and holding times to find the optimized heat treatment conditions and it was analyzed by linking the microstructural characteristics and mechanical properties. The part of β phase with 10 ± 2 wt% vanadium was transformed into α```` martensite phase after quenched, so the hardness and tensile properties were decreased below 900℃. The higher the heat treatment temperature is, the smaller is the vanadium-rich region, which leads to transformation into hcp α`` martensite above 900℃. The hardness and tensile properties were improved due to the hard α`` martensite. As the holding times were longer, the hardness and tensile properties decreased below 900℃ because of the softening effect by the grain growth. When varying the holding times above 900℃, the change of mechanical properties was slight because the softening effect of grain growth and the strengthening effect of α`` phase were counteractive. Therefore, the best conditions of heat treatment, which is in the range of 920~960oC, 40 min, WQ, can effectively improve the mechanical properties of Ti-6Al-4V. (Received August 5, 2016; Revised August 25, 2016; Accepted September 5, 2016)
조성근(Sungkeun Cho),김진영(Jin Yung Kim),이광근(Kwangkeun Yi) 한국정보과학회 2011 한국정보과학회 학술발표논문집 Vol.38 No.2A
이 논문에서는 정적으로 2단계 언어의 불필요한 식을 안전하게 찾는 간섭분석 방법을 제안한다. 불필요한 식이란 식의 실행이 전체 프로그램의 실행 결과에 영향을 미치지 않는 식을 말하고 간섭분석이란 프로그램의 최종 결과에 영향을 미치는 식들을 찾아내는 분석을 말한다. 정적으로 불필요한 식을 찾으면 전체 프로그램 실행에 드는 비용을 줄일 수 있다. 이 분석은 타입 시스템에 올라탄 분석에 기반한다. 타입 규칙을 디자인하고 안전성을 증명하였으며 이를 위한 알고리즘을 디자인하고 올바름을 증명하였다. 또한 다형 타입을 지원하도록 타입 규칙을 확장하였으며 그 안전성도 증명하였다.
백정애(Jungae Baik),김인경(In Kyoung Kim),김진영(Jin Yung Kim),노용환(Yong Hwan Noh),배선영(Sunyoung Bae),우명원(Myoung Won Woo),장혁기(Hyukki Chang),조성돈(Sung Don Cho),장매희(Maehee Chiang) 인간식물환경학회 2011 인간식물환경학회지 Vol.14 No.3
This study was investigated to research the basis of interdisciplinary fusion through the survey on the basis of the answers of professionals and general public, gender, humanities, science and engineering about the question of climate change and chimera which is one of the various phenomena that affect the human life. All respondents’ perceptions of climate change was high and natural disasters was showed the most destruction according to climate change. The answer of awareness of climate change and the damage, chimerism was significant differences between age groups sex, specialized domain-specific individuals. In addition, a group of respondents for science and engineering professionals was higher for awareness of climate change and the chimerism and heir negative opinion for chimerism was relatively low in comparison of other research groups.
금불초 추출물의 항산화 효과 및 산화 스트레스에 대한 신경세포 보호작용
이나현(Na Hyun Lee),홍정일(Jungil Hong),김진영(Jin Yung Kim),장매희(Mae-Hee Chiang) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.1
본 연구에서는 금불초(Inula britannica) 추출물의 항산화 효과와 H₂O₂로부터 유도된 SH-SY5Y 신경모세포종의 세포독성에 대한 보호능을 측정하였다. 금불초 지상부위의 70% 메탄올 추출물에 대하여 용매별로 분획을 실시하였고 핵산(Fr.H), 에틸아세테이트(Fr.EA) 및 물(Fr.W) 분획에 대하여 활성을 조사하였다. 분획중 Fr.W의 폴리페놀/플라보노이드 함량이 가장 높았으며 Fr.W의 총 폴리페놀 함량은 318.1±20.6 ㎍/㎎ solid로, Fr.EA 및 Fr.H와 비교하여 각각 약 2.5배, 23.1배 수준이었다. DPPH radical, ABTS radical 및 nitric oxide 소거능 등의 항산화 활성에서도 Fr.W가 가장 높은 활성을 나타내었고 Fr.H는 거의 활성을 나타내지 않았다. Fr.W는 H₂O₂에 의해 유도된 세포사멸에 대하여 62.5 ㎍/mL 농도에서 현저하게 세포독성을 감소시켰으며 250 ㎍/mL에서는 77.0%의 세포사멸 억제능을 보였다. Fr.EA는 보호 효과를 나타내지 않았으며 Fr.H는 오히려 H2O2로 인한 세포 독성을 증가시키는 것으로 나타났다. 세포 내 ROS에 대한 영향으로 Fr.W 250 ㎍/mL 처리시 39.2% 세포내 ROS를 감소시켰으며 Fr.EA는 25 ㎍/mL에서 26.8%의 세포내 ROS를 소거하였다. 이러한 금불초 Fr.W의 항산화 활성은 ROS에 의해 야기되는 뇌세포 독성에 대한 보호 작용에 공헌할 수 있을 것으로 예상된다. The antioxidant properties and protective effects of Inula britannica on H₂O₂-induced SH-SY5Y neuroblastoma cell damage were investigated. A series of solvent fractions, including hexane(Fr.H), petroleum ether, chloroform, ethyl acetate(Fr.EA), and water fraction(Fr.W), were prepared from the 70% methanol extracts of Inula britannica. Fr.W had the highest total contents of phenolics and flavonoids, followed by Fr.EA. The antioxidant properties of the fractions were also evaluated by analyzing their scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals, 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and nitric oxide. Fr.W showed the strongest activities in all assays. The concentrations of Fr.W that resulted in 50% reductions of the DPPH and ABTS radicals were 20.7 ㎍/mL and 39.4 ㎍/mL, respectively. Fr.W showed the weakest cytotoxic activities on the SH-SY5Y cells, whereas it effectively protected H₂O₂-induced cell death, increasing cell survival by 35.0-77.0% at a concentration range of 62.5-250 ㎍/mL. In this range, Fr.W also significantly decreased intracellular ROS levels by 34-39%. Overall, the antioxidant properties of Inula britannica can contribute to rescuring neuronal cells from oxidative stress-induced cell injury.