Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

청소년 도덕적 동기화 검사 도구 개정 연구(2차년도)

전종희 ( Jong Hee Jeon ),이인재 ( In Jae Lee ),김영한 ( Young Han Kim ),박균열 ( Gyun Yeol Park ),윤영돈 ( Young Don Yoon ),류숙희 ( Sook Hee Ryue ),홍성훈 ( Seong Hoon Hong ) 한국윤리교육학회 2013 윤리교육연구 Vol.0 No.29

본 연구는 J. Rest의 4구성요소 모형을 바탕으로 1차년도(2011년)에 개발된 도덕적 동기화 검사를 보다 신뢰롭고 타당한 도구로 개정하는 것이다. 따라서 선행연구를 보완하여 도덕 동기화의 구성 요소를 도덕지향성과 도덕적 정서귀인 등 두 항목으로 재설정하고, 스토리와 문항을 수정하였다. 개발된 세 개의 스토리(과제, 놀이공원, 작품) 별로 신뢰도를 분석한 결과, 전반적으로 .90 내외의 높고 수치를 보였다. 응답의 편포성을 완화하기 위해 2차년도에는 7점 척도를 사용하였다. 예비조사를 위해서는 전국 중학교 2학년생 409명을 대상으로 했고, 본조사를 위해서는 전국 6,216명의 중학교 2학년생을 대상으로 실시하였다. 개발된 검사도구의 신뢰도계수(문항 내적 일치도인 크론바흐 알파)는 .90이상으로 신뢰할만했다. 타당도를 검증하기 위하여 요인분석을 실시한 결과, 과제 스토리와 놀이공원 스토리의 일부 문항이 가정한 요인으로 잘 묶이지 않았는데 이 문항들을 제거하고 요인분석을 다시 실시해본 결과 기대하는 바를 충족했다. 그리하여 2차년도 연구를 통해 최종 스토리는 총 세 개(과제, 놀이공원, 작품)로 확정되었고, 각 스토리별 문항수는 과제 9개 문항, 놀이공원 9개 문항, 작품 10개 문항으로 총 28개 문항으로 확정되었다. 본 연구를 통해 수정 확정된 도덕적 동기화 검사도구는 상당히 안정적인 신뢰도와 타당도를 확보하였기에, 향후 우리나라 청소년의 도덕적 동기화 능력을 측정하기 위한 검사도구로 활용되기에 적절할 것으로 판단된다. The study aims to revise Moral Motivation Test I which was set for adolescents based on Rest`s four component model in 2011 and make standardized final morality test. In theory, this study is based on 4 components theory of James Rest who invented DIT (Defining Issues Test) to measure the moral judgment. The 4 components are composed of moral sensitivity, moral judgment, moral motivation, and moral character (or moral implementation, moral action). This study focused on moral motivation. This 2nd year study could get some significant outcomes. To make test form, 3 components relating teams have closely exchanged informations. Because teams have to share same real life stories as follows: “homework inspect”, “on going to park”, “broken art work”. The questionnaire used seven likert type to lessen the far epistemological tendency. To represent moral motivation, research team used moral orientation, moral emotion attributions. To get final form of morality test, this study was made two sequential steps. First one was pre-test which was for 409 respondents. Second main one was for 6,216 respondents. For moral motivation, the reliability was highly coefficient with Cronbach`s alpha(>.90). The validity was also much improved and stable. But just in the story “homework inspect”and “on going to park”, the factor analysis shows a little bit limitation result. Eliminated those questions, the result of factor analysis was more stable and reliable. Through these processes, to test moral motivation, this study shows 3 stories and 28 questions. And this recommends that just one simple story might be developed for one component(moral motivation).

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

직장 구조조정과 파업에 의한 근로자의 사회심리적 스트레스의 변화

박봉진,이종태,손혜숙,김성준 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : This study was conducted to evaluate the insecurity of employment and anxiety by downsizing and strikes affected job characteristics and psychosocial distress of workers. Methods and Material : This is a longitudinal study of the container terminal workers in Pusan. The company operated downsizing from October to December in 1999 by economic depression. In the result of that, the number of workers decreased into 627 from 662. During that period, a new union was built and it made conflict between employers and employees, so the conflict caused the strikes from February to April in 2000. The first cross-sectional study was performed in February, 2000 and the oneyear follow-up cross-sectional study was performed in February, 2001. Socio-demo graphic characteristics, stress-related behavior, job characteristics and psychosocial distress were assessed with job content questionnaire(JCQ) and Psychosocial well-being Index(PWI) in 200 workers(white-collar: 100 men, blue collar: 100 men) from 662 men in total. Results : In the first study, job strain was high in the whole, but there was no difference between white and blue-collar workers. The level of PWI was very high at 86.67 points and the level of blue-collar workers was higher than that of white-collar workers(p=0.013). In the results of follow-up study, the rate of drinking and smoking decreased as the change of stress-related behaviors. In the job characteristics change, decision authority and physical exertion of the white-collar workers got lower(p<0.05), but social support and job strain didn't have no difference. In the PWI level change, stress level got rather higher(p<0.01), especially, increase of the white-collar workers' stress was big difference from that of the blue-collar workers'. Conclusion : Job stress management programs should be made to reduce stress. And in approaches to management and prevention, excessive physical exertion, shift work and irregular meals and breaks should be considered. Especially, this stress control program should be begun with recognition on conflicts between employers and employees and between employees and employees by downsizing and strikes. And also efforts to reinforce social support should be needed.

Helium-Oxygen Plasma 조사에 의한 방사능 저항 미생물 Deinococcus radiodurans 살균

박연희,김성미,김종일 서울여자대학교 자연과학연구소 2006 자연과학연구논문집 Vol.18 No.-

다양한 살균법이 있지만 열에 약한 물질을 살균 처리하는 방법으로는 여러 문제점을 지닌 ethylene oxide(EtO) gas를 이용한 것이 주로 쓰이고 있는 실정이다. 플라즈마를 이용한 살균법은 EtO 살균법의 단점을 보완해 주는 대체 기술로 제시되고 있는데 플라즈마 조사에 대한 미생물의 생존을 UV 조사와 비교 분석하였다. 플라즈마에 직접 노출된 Deinococcus radiodurans는 조사 시간의 증가에 따라 colony 형성능력이 크게 감소하였으며, UV 조사에 의한 사멸보다 더 효과적으로 작용하였다. 또한 세균에 플라즈마를 쪼여 준 후 생체 내 효소와 세포 사멸에 관계가 많은 산소 활성종의 양이 증가하여 세포 사멸에 큰 영향을 준다고 사료된다. There are various methods for sterilization but the heat-sensitive materials are sterilized usually by ethylene oxide gas, which has several problems. Plasma sterilization method could replace the sterilization with ethylene oxide gas. The use of plasma for sterilization has been analyzed in this study. The extents of surving colonies imparted by plasma exposure for the increasing time were assessed. The killing effect by plasma was exhibited to be much higher than those by exposure to UV. Irradiation of plasma to the cells was the cause of the breakdown of biopolymer via action of reactive oxygen species, which could result in the cell death.

고강도 할로겐광으로 중합한 복합레진 수복재의 경도

박종석,이광희,김대업,김성형,안호영 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

복합레진의 광중합시간 단축과 관련하여 최근에 개발된 고강도 할로겐광 중합기를 기존의 중강도 할로겐광 중합기와 비교 평가하기 위하여. 세 종류의 광중합용 복합레진을 두께가 2, 3, 4, 5mm인 스테인레스 스틸 몰드에 충전하고, 레진 상면을 중강도 할로겐 광은 (1) 400mW/cm²으로 40초간, 고강도 할로겐광은 (2) 10초동안 100mW/cm²부터 1000mW/cm²까지 출력량이 증가하면서 중합하고 다음 10초동안 1000mW/cm²로 중합하는 ramp 모드로 20초간, (3) 1000mW/cm²의 boost 모드로 10초간. (4) 850mW/cm²의 표준 모드로 20초간 조사하고. 상면과 하면의 표면경도를 미세경도측정기로 측정한 후 실험군간의 차이를 분석하였다. 레진 상면의 경도는 중합광의 강도와 중합시간 및 레진 두께에 따른 차이가 없었다(유의수준 0.05, 이하 동일). 레진 하면의 경도는,두께 2mm의 중강도군(1)을 제외하고,상면의 경도보다 낮았다. 레진 하면의 경도는 두께 2mm에서 중강도군(1)이 ramp군(2) 및 boost군(3)보다 높았고 중강도군(1)과 표준군(4) 간 및 ramp군(2)과 boost 군(3)간에 차이가 없었으며 두께 3mm에서 중강도군(1)이 가장 높았고 표준군(4)이 boost군(3)보다높았다. 따라서, 복합 레진의 중합 깊이 측면에서 볼 때. 1000mW/cm²의 고강도 할로겐 광을 10초간 조사한 것은 400mW/cm²의 중강도 할로겐광을 40초간 조사한 것에 미치지 못하였으며, 레진 두께 2mm이내인 경우에는 850mw/cm²의 고강도 할로겐 광을 20초간 조사함으로써 400mw/cm²중강도 할로겐 광을 40초간 조사한 것과 대등한 중합을 일으킬 수 있었다. The purpose of this study was to compare the effect of the high intensity halogen light (850∼ 1000 mW/cm²) with that of the conventional halogen light (400 mW/cm²) on the hardness of composite resin. Three resin com-posites (Z-100, 3M, U.S.A ; Tetric Ceram, Vivadent, Liechtenstein : Surefil, Dentsply, U.S.A.) were filled in the stainless steel moulds which were 4mm in diameter and 2, 3, 4, and 5 mm in depth, respectively. They were cured under the four different modes : (1) conventional mode,40 seconds at 400 mW/cm²; (2)'ramp'mode, 10 seconds at 100 to 1000 mW/cm² plus 10 seconds at 1000 mW/cm²: (3)'boost'mode, 10 seconds at 1000 mW/cm²: and (4) 'standard'mode, 20 seconds at 850 mW/cm² The surface hardnesses of the top and the bot-tom of the resin samples Were measured with a microhardness tester (MXT70, Matsuzawa, Japan) . The top surface hardness was not significantly different among the curing modes . The bottom surface hardness was gen-erally the highest in the conventional mode and the lowest in the high intensity boost mode There was no sig-nificant difference in the bottom surface hardness between the conventional mode and the high intensity standard mode in 2mm depth. The results suggest that the curing time of the high intensity halogen light (850 mW/cm²) should be at least 20 seconds to produce the equal level of the bottom surface hardness of 2mm resin composite as compared to the hardness produced by the conventional halogen light (400 mW/cm²).

프리캐스트 상판 그라우트형 횡연결부의 전단강도

박종진,오성호,신수봉 동아대학교 건설기술연구소 2001 硏究報告 Vol.25 No.1

Precast R.C. slabs are gaining an acceptance for the construction of bridge structures because of simplicity in their construction processes. One of the frequently encountered problems with precast slabs is design of transverse joint. In this study, grout type transverse joints between precast concrete slabs are statically tested to determine the cracking loads and ultimate shear capacities. The tests are performed with a loading equipment especially designed and constructed in the lab to induce shear failures on the joints of the test specimens. Shape of the transverse joints, grouting materials and amount of prestress are selected as test parameters for the study. The results indicate that epoxy is an excellent grouting material which can be sued in limited locations where large tensile stress is acting on the slab. Longitudinal prestressing is also an effective method to increase the shear strength of the transverse joints. A rational method to estimate the cracking and ultimate loads for the design of grout type transverse joint is proposed based on the static loading test.

간호사의 근골격계질환 증상유병정도 및 관련요인

박종,강명근,이영선,강성득,김철호 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

Objectives : The purpose of this study were to identify musculoskeletal symptom among nurses in a hospital and to explore its related factors . Methods : A self-administered questionnaire survey including work condition and presence of musculoskeletal symptom was made toward 190 nurses at a university hospital. Results : The prevalence of musculoskeletal symptom was calculated as 20.4% at neck, 27.7% at shoulder, 15.7% at arm or hand and 29.3% at leg. By multiple logistic regression, the prevalence of musculoskeletal symptom was 3.57 times (95% CI : 1.53 - 8.35) higher among persons with general illness symptom than persons without symptom, 2.99 times (95% CI : 1.22-7.36) higher among persons with psycho-rieuro subjective symptom than without symptom, 3.48 times (95% CI : 1.46 - 8.27) higher among irregular menstruation period than regular period. The subjective symptom prevalence at the shoulder was 2.24 times (95% CI : 1.06 - 4.71) among persons with past history of certain chronic diseases than without the history. The prevalence at the upper arm was 4.60 times (95 % CI : 1.24 - 17.08) higher among persons drinking two cups of coffee a day and 9. 92 times (95 % CI : 2.28 - 34.91) higher than persons who drank a cup of coffee a day or less. The prevalence of backache was 3.18 times (95 % CI : 1.10 - 11.01) higher among 30-34 year old group than group less than 25 years old persons. The prevalence of subjective symptom at leg showed 3.93 times (95% CI : 1.44 - 10.71) higher among persons with irregular menstruation period than regular period. Conclusion : Many nurses under study had musculoskeletal symptom and the symptom was related with complicated combination of general characteristics, subjective symptom at other site, occupational characteristics and characteristics related with diseases, by various site of the system. Therefore appropriate intervention programs are needed ot be developed to prevent musculoskeletal illness among nurses.