High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

심인성 색전 요인이 없는 뇌경색 환자에서 경식도 심초음파와 Acoustic Quantification을 이용한 대동맥 탄성의 평가

이종민,윤호중,임효영,박지원,김휘열,전희경,문건웅,정욱성,채장성,김재형,최규보,홍순조 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

5-〔3-(N-Substituted Piperazinyl)Propoxy〕Indan類의 合成과 中樞神經抑制效果

黃熙喆,閔庚鎭,文勝煜,李萬佶,徐丙天 嶺南大學校附設 基礎科學硏究所 1985 基礎科學硏究 Vol.5 No.-

Five new indans, 5-[3-(N-substitued piperaziny) propxy]indans, were synthesized by condensation of 5-(3-bromopropoxy)indan, the intermediate, and N-substituted piperazines. The structure of the compunds were determined by means of IR, NMR spectoscopy along with elemental analysis. Each compound was tested for antimorphine and antiamphetamine effects as part of CNS depressant effects and for muscle relaxant action in mice. Thes compound exhibited less potent activity compared to chlordiazepoxide on CNS depressant effect. Muscle reaxant action of the compounds, the side effect of tranquilizer, however, was much weaker than that of chlordiazepoxide.

자돈에 있어 게르마늄 용질액의 급여가 성장 및 혈액학적 변화에 미치는 영향

홍종욱,권오석,민병준,조진호,진영걸,손경승,강종옥,김인호 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

본 연구의 목적은 이산화게르마늄을 함유한 게르마늄 용질액을 자돈에 급여하였을 때 성장 및 혈액학적 변화에 미치는 영향을 조사하기 위하여 실시하였다. 3원 교잡종 자돈 60두 (평균체중 11.22±0.10㎏)를 공시하였다. 시험설계는 옥수수-대두박 위주의 대조구(CON)와, 대조구 사료내 게르마늄 용질액을 0.5 ppm 첨가한 구(GC0.5) 및 대조구 사료내 게르마늄 용질액을 1.0 ppm 첨가한 구(GC1.0)로 3개 처리를 하였다. 0-10일간 사양시험기간동안, 일당증체량에 있어서 GC0.5 처리구가 약간 높게 평가되었다(quadratic effect, P<0.02). 그러나 전체 시험기간동안, 일당증체량, 일당사료섭취량 및 사료효율에 있어서 처리구간에 유의적인 차이는 보이지 않았다. 영양소 소화율에 있어서는 GC0.5 처리구가 조회분(linear effect, P<0.01; quadratic effect, P<0.04), 칼슘(linear effect, P<0.01; quadratic effect, P<0.01), 인 (linear effect, P<0.02; quadratic effect, P<0.04) 소화율이 유의적으로 높게 평가되었다. 혈액내 neutrophil 농도에 있어서는 게르마늄 용질액의 첨가 수준이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다(quadratic effect, P<0.02). 결론적으로, 자돈 사료내 게르마늄 용질액의 급여가 성장 및 혈액학적 수치에는 영향을 미치지 못하는 것으로 사료된다. This study was conducted to investigate the effects of dietary germanium colloid on the growth performance and hematological values in nursery pigs. Sixty pigs (11.22(0.10 ㎏ average initial body weight) were used in a 20 d growth assay. Dietary treatments included 1) CON (basal diet), 2) GC0.5 (basal diet+0.5 ppm germanium colloid) and 3) GC1.0 (basal diet+1.0 ppm germanium colloid). For d 0 to 10, pigs fed GC0.5 diet grew faster than pigs fed CON and GC1.0 diets (Quadratic effect, P<0.02). However, through the entire experimental period, no statistical differences were found for aver-age daily gain, average daily feed intake and gain/feed. Apparent digestibilities of crude ash (Linear effect, P<0.01; Quadratic effect, P<0.04), calcium (Linear effect, P<0.01; Quadratic effect, P<0.01) and phosphorus (Linear effect, P<0.02; Quadratic effect, P<0.04) in pigs fed GC0.5 diet were greater than for pigs fed CON and GC1.0. Neutrophil concentration in blood increased as the concentration of germanium colloid in the diets was increased with significant difference (Quadratic effect, P<0.02). In conclusion, growth performance and hematological values were not influenced by dietary germanium colloid.

제주도 함덕연안의 자치어 출현과 군집구조 : 일변동

고경민,문상욱,고유봉 제주대학교 해양연구소 1996 해양자원연구소연구보고 Vol.20 No.-

The occurrence and community structure of fish larvae at the coastal area of Hamduck. Cheju Island have been studied through the hourly multi-layer sampling with a MTD net for 24 hours on August 10~11, 1989 (at just one site). The multi-layer samplings show that the number of species and individuals at the 1Om and 15m depths are more than those at the surface : the total number of species being 28, the number of species at each depths being 9, 19 and 21 at the depths of 0m, 10m and 15m, respectively. The major fish larvae are chromis notdudtotal 36,454/1000㎥ indv., 92.8%). Enneupterygius etheostomus (total 855/1000㎥ indv., 2.2%, Callionymidae sp.(total 489/1000㎥ indv.. 12%). etc At the surface and the 10m depth the number of individuals changed vividly in time and the largest number of individuals appeared at midnight. This resulted from vertical migration which was caused by the rise of fish larvae living at the 15m depth. At the surface the fish larvae descended about the sunrise and at the 10m and 15m depths they desce-nded slowly after the sunrise. The species diversity of the fish larvae at the 10m and 15m depths was higher than that at the surface and generally was higher in the nighttime than in the daytime. The variation of these values seemed to have a close relationship with the change between the species number and individuals at that time. As a result of clustering analysis at each depth, Gobiidae sp. and Callionymidae sp. occurring at about midni-ght showed close similarity(0.67) at the surface, while three groups represented 0.50 of similarity at the 10m and 15m depths.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

의미 치매 1예 : 신경심리 및 뇌영상 검사의 연속적인 추적 관찰 소견 Serial Follow-up Study of Neuropsychological and Neuroimaging Findings

박경원,김욱주,전진민,강도영,김재우 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Semantic dementia (SD) is a very rare, distinct form of dementia which is characterized by impairment of naming and comprehension, prosopagnosia, visual agnosia for objects and relative preservation of other aspects of cognition, such as episodic memory, nonverbal problem-solving and visuospatial functions. A 68-year-old man visited our clinic for progressive speech disturbance since 1997. His main speech problems were word finding difficulty and loss of word meaning for objects. Language assessment demonstrated profound semantic memory loss for words with preserved fluency, corresponding to anomic aphasia. There were marked impairments in verbal and visual memory functions and word fluency tasks, but no grammatical or phonologic errors in speech. Also were remarkable anosognosia and object visual agnosia with preserved visuospatial function and calculation in neuropsychological tests. Serial follow-up study of language test showed more deterioration in comprehension and repetition, consistent with Wernicke's aphasia. Initial brain MR imaging and brain perfusion SPECT showed prominent focal atrophy in the left temporal lobe, and marked hypoperfusion in the left temporal and both frontal areas. Follow-up brain MR imaging and perfusion SPECT revealed more atrophy in both temporal lobes and showed more extensive hypoperfusion areas compared to the previous study.

삼일열 말라리아에 합병된 비장 파열 1예

홍경욱,이정아,김원진,박철민,권혜리,박혜원,엄중식 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

국내의 토착형 말라리아는 삼일열 말라리아로, 1993년부터 재유행이 시작되었다. 말라리아의 중요한 합병증으로 드물지만 비장 파열이 있는데, 이는 주로 삼일열 말라리아에 의해서 발생되며, 국내에서는 1999년 말라리아에 의한 비장 파열로 비장 절제술을 시행한 예가 보고된 바 있다. 현재까지는 비장 파열이 있을 때 비장 절제술이 일차적인 치료로 되어 있으나, 비장 절제술 후의 감염 위험성이 크므로, 활력 징후가 불안정하거나, 출혈이 지속되는 소견이 보이지 않으면 가급적 보존적 치료를 하는 것이 추천된다. 저자들은 비장 파열이 동반된 말라리아 환자에서 비장 절제술을 시행하지 않고 보존적 치료로 호전된 예를 경험하여 보고하는 바이다. Plasmodium vivax has been the predominant Plasmodium species in the Republic of Korea and reemerged in 1993. Spontaneous rupture of malarial spleen is a rare but important complication associated with P. vivax infection. Only one case of spontaneous rupture of malarial spleen was reported in Korea in 1999. Splenectomy is still accepted as the treatment of choice in cases of spontaneous rupture of the spleen. But, considering increased risk of postsplenectomy infecion, splenectomy should be reserved for those patients with severe rupture or those with continued or recurrent bleeding. And conservative treatment should be considered in selected, closely monitored patients. We report a patient with P. vivax malaria who developed a spontaneous splenic rupture and was improved by conservative treatment.

상부요로계감염 환자에서 Gallium-67 Citrate 스캔 및 Tc-99m Dimercaptosuccinic Acid(DMSA)스캔의 임상적 의의

이강욱,강민규,장윤경,양종오,이상주,박기현,소영,신영태 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

It is often not certain whether the focal defect of renal cortex of Tc-99m Dimercaptosuccinic acid(DMSA) renal scan in patients with acute pyelonephritis means the cortical scar by previous inflammation or recent active inflammatory lesion. Gallium-67 citrate(Ga-67) also has been known to be accumulated at the active inflammatory lesion. In order to evaluate the usefulness of the Ga67 and Tc-99m DMSA renal scan in patients with acute pyelonephritis, we reviewed the results of both study and clinical findings. Nintecn patients with typical clinical findings of acute pyelonephritis and admitted to our institute from Jan. 1998 to Nov. 1998 were included in this study. Tc-99m DMSA and Ga-67 scan were performed mean 3.3 and 7.3 days after admission. Eleven of 19 patients (57.9%) showed abnormally increased focal renal uptake on the Gallium-67 citrate scan and 14 of 19 patients(73.7%) had a cortical defect on the kidneys in Tc-99m DMSA scan. Seven(36.8%) patients had an abnormality in both study. Seven(36.8%) patients had an abnormality only in Tc-99m DMSA scan and 4 (21.1%) patients had an abnormality only in Ga-67 scan. Of 7 patients who had an abnormality on both scan, 5 patients had. an abnormality at same region in both scan and 2 patients had an abnormality at the different sites on the kidneys. With the above results, we speculate that Ga-67 scan and Tc-99m DMSA scan play a complementary role in the patients with upper urinary tract infection.

Thiobacillus ferrooxidans를 이용한 소화 슬러지의 중금속 제거에 미치는 슬러지 농도의 영향

류희욱,김윤정,조경숙,강근석,최형민 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

철산화세균인 Thiobacillus ferrooxidans를 이용하여 폐수처리과정에서 발생되는 혐기소화 슬러지의 생물학적 중금속제거에 ㅍ미치는 슬러지 농도의 영향을 조사하였다. 혐기소화 슬러지와 탈수슬러지에 적용이 가능하도록 슬러지 농도를 2, 5, 9, 17%(w/v)로 설정하였다. 5%(w/v)이상의 슬러지 농도에서 T. ferrooxidans의 중금속 용출율이 매우 낮았다. T. ferrooxidans의 철산화능은 슬러지 용출액의 농도가 증가할 수록 저해받았고, 특히 슬러지 농도가 6%(w/v)이상인 슬러지 추출용액에서는 균의 활성이 완전히 저해받았다. 고농도 슬러지에서의 T. ferrooxidans의 활성저해는 물질전달의 제약보다는 용출액에 함유된 유기물이나 다른 용존저해물질의 작용이 주요 원인임을 알수 있었다. T. ferrooxidans는 혐기소화 슬러지액과 같이 고형물질의 양이 1.3∼4.0%(w/v)의 낮은 슬러지 농도에만 효과적으로 적용이 가능하였다. To investigate the feasibility of the microbial process for removal of heavy metals from the high solid content sludge, the effect of sludge concentration on the solubilization of heavy metals by an iron oxidizing Thiobacillus ferrooxidans was examined. With increasing the sludge concentration, the removal efficiency of heavy metals and the oxidation rate of iron were inhibited. Especially, when the sludge concentration is over 5%(w/v), the activity of T. ferrooxidans was remarkably inhibited. This inhibition is considered to occur due to the dissolved inhibitory materials such as organic compounds, heavy metals, and others which were extracted from the sludge during incubation period. In conclusion, the microbial process by T. ferrooxidans is only effectively used in ranges of 1.3 to 4.0%(w/v) sludge concentration.