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문상욱,정병길 한국환경기술학회 2022 한국환경기술학회지 Vol.23 No.1
The purpose of this research was to compare the RO membrane performance such as permeate flow rate, flux, recovery ratio according to the membrane CIP(Clean-In-Place) methods(One-way CIP and two-way CIP). The seawater used in this research was sampled at a depth of 3∼5 m in the sea located in B City. The average water quality of seawater was found to be turbidity of 1.28 NTU, TDS 30,335 ㎎/L, hardness 3,330 ㎎/L as CaCO3, and SO42- 1,890 ㎎/L. The flux of the new RO membrane, fouled RO membrane, one-way CIP membrane and two-way CIP membrane were 97.3 L/m2·hr, 66.5 L/m2·hr (recovery ratio : 68.0 %), and 81.1 L/m2·hr (recovery ratio : 83.3 %) and 94.1 L/m2·hr (recovery ratio : 96.7 %), respectively. It was found that the two-way CIP membrane washing method showed higher washing efficiency than the one-way CIP membrane washing method.
카이이케호에서 농밀하게 분포하는 Purple Sulfur Bacterium의 수소생산
문상욱,마쓰야마 미치로 ( Sang Wook Moon,Michiro Matsuyama ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.1
H₂ production by Chromatium sp., a large purple sulfur bacterium blooming in lake Kaiike, under various environmental conditions was examined. Chromatium sp. produced Hz only in the presence of light and HZS. Maximum H₂ production (0.01 μ㏖/hr/(㎎ dry cell weight)) was obtained in the solution of 20 ㎎ H₂S-S/ℓ under low light intensity (1000 lux) at 30℃. H₂ production was severely inhibited by the presence of N₂ or NH₄^+. The rate observed for Chromatium sp. was relatively low compared to that of other phototrophic bacteria. Chromatium sp. is probably a most potent H₂ producing species in lake Kaiike, since the bacterium readily produced H₂ photoautotrophically even at low light intensities by the application of suboptimal H₂S concentrations. Based on the photoautotrophic characteristics of bacterial Hz production, it is suggested that Chromatium sp. can be an economic and practical species for biological H₂ production system, particularly in temperate region.
양어용 사료첨가제로서 감귤발효액 (EM-Fermented Orange)의 항산화특성
문상욱,이영돈,이준백,고유봉 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-
Mandarine orange (Citrus unshiu Marc.) only added with sugar was fermented by effective microorganisms (EM). called "EM-fermented orange". EM was mainly composed of lactic acid bacteria. phototrophic bacteria and yeast. As pH of EM-fermented orange reached 3.5 to 4.0 in the course of fermentation. the incubation was stopped. and then. EM-fermented orange was preservable at room temperature for about 6 months. Composition and concentration of free amino acids in EM-fermented orange were analyzed. Concentration of total amino acids was 167 mg/l. and the relative content of carnosine (β-alanyl-L-histidine) which was well known as a kind of antioxidant materials was very high. 41%. In addition. crude extract showing antioxidant activity could be obtained from EM-fermented orange using some organic solvents. The antioxidant activity of the crude extract was equivalent to about 9% of that of vitamin E by the use of 1.1-diphenyl-2-picrylhydrazyl (DPPH) method. It was suggested that EM-fermented orange having antioxidant characteristics could promote the health of fish.
버섯균사체 배양기질로서의 손바닥선인장의 활용과 그 배양추출물의 기능적 특성
문상욱,박수영,최수연,황준호,장미경,진영준,정완석,김세재,Moon Sang-Wook,Park Soo-Yeong,Choi Soo-Youn,Hwang Joon-Ho,Jang Mi-Kyoung,Jin Yeong-Jun,Chung Wan-Seok,Kim Se-Jae 한국생명과학회 2006 생명과학회지 Vol.16 No.3
본 연구는 버섯균사체의 배양 기질로서 손바닥선인장 열매의 활용 가능성을 타진하기 위하여 수행하였다. 5 종류(신령, 잎새, 노루궁뎅이, 차가, 상황)의 버섯균사체를 MEB배지와 손바닥선인장 열매 액체배지(CB)에서 각각 배양하여 에틸아세테이트로 추출한 후, 각 추출물의 수율, 총 폴리페놀 함량, 그리고 몇몇 생리활성을 분석하여 비교하였다. CB 배지에서 배양한 버섯 균사체 중에 잎새버섯 균사체가 추출 수율(0.4 g/l)과 총 폴리페놀 함량(22.7%)에서 가장 양호하였다. CB 배지에서 배양한 버섯 균사체 추출물들은 항산화 활성(DPPH 자유기 소거활성, superoxide의 소거활성, xanthine oxidase 억제 활성), LPS로 활성화시킨 RAW264.7세포에서의 NO 생성 억제 활성, 항균활성, 그리고 HL-60에서의 세포증식 억제 활성 모두가 MEB 배지에서 배양한 버섯균사체 추출물의 것과 비교해 현저하게 증진되어 있음을 확인할 수 있었다. 특히, 잎새버섯 발효추출물에서 DPPH 자유기 소거활성($IC_{50}=362.9{\mu}g/ml$), xanthine oxidase 저해활성(80% at $500{\mu}g/ml$), superoxide 소거활성(80% at $500{\mu}g/ml$), NO 생성 억제 활성($IC_{50}=43.1{\mu}g/ml$), 항균활성, 그리고 HL-60 세포 증식 억제 활성 등 분석된 모든 생리활성이 가장 높게 나타났다. 본 연구 결과는 손바닥선인장 열매가 버섯균사체의 기능성 증진을 위한 배양기질로서 활용될 수 있음을 시사하였다. This study was performed to know the potentialities of the fruits of Opuntia ficus-indica, as a medium for mushroom mycelial culture. Five mushroom mycelial (Agrocus blazei, Grifola frondosa, Hericium erinaceum, Innonotus obliquus, Phellinus linteus) were frown on the malt extract broth (MEB) and the cactus broth medium (CB). The submerged culture mixtures were extracted using equal volume of ethyl acetate, and their extract yields, total polyphenol contents, and some physiological activities were compared with each other Each extract from mycelial culture grown on CB medium showed remarkable enhancement in physiological activities compared with each counterpart grown on MEB. Among five mycelial cultures grown on CB medium, the extract yield and polyphenyl content were highest in the extract from Grifola frondosa (extract yield, 0.4 g/L and polyphenol content, 22.7%). Also, the extracts from Grifola frondosa showed the highest physiological activities, such as DPPH radical scavenging ($IC_{50}=362.9{\mu}g/ml$), xanthine oxidase inhibition (about 80% at $500{\mu}g/ml$), and superoxide radical scavenging (about 80% at $500{\mu}g/ml$), and NO production inhibition ($IC_{50}=43.1{\mu}g/ml$) in LPS-stimulated RAW 264.7 cells. This result suggests that the fruit of Opuntia ficus-indica can be used as a culture medium for improving the functional properties of various mushroom mycelia.
해수담수화를 위한 나노버블 침투형 복합 하이드로싸이클론 전처리 기술개발
문상욱 ( Sang-uook Moon ),최호은 ( Ho-eun Choi ),최영익 ( Young-ik Choi ),성낙창 ( Nak-chang Sung ),정병길 ( Byung-gil Jung ) 한국환경기술학회 2020 한국환경기술학회지 Vol.21 No.6
The purpose of this research was to develop a nanobubble multihydrocyclone pretreatment technology that supplies SDI (Silt Density Index) of below 3 in order as alternative to, and etc. the existing coagulation filtration pretreatment process in seawater desalination. The used seawater in this research was sampled at a depth of 5 m in the sea located in B City. The average water quality of seawater was found to be turbidity of 1.28 NTU, TDS 29,800 mg/L, hardness 3,330 mg/L as CaCO<sub>3</sub>, total colony counts 190 CFU/100 mL, total coliforms 1,400 CFU/100 mL, and SO<sub>4</sub> <sup>2-</sup> 1,890 mg/L. The nanobubble multi-hydrocyclone pretreatment process was consisted of a nanobubble generator, hydrocyclone, carbon filter, microfilter, RO pump, RO and UV systems. As a result of measuring (average of 5 times) the SDI of raw seawater, pretreated water, secondary treated water and final effluent were found to be 5.74, 3.42, 2.88 and 0.27, respectively. It was satisfied the SDI standard value of below 5 for seawater desalination pretreatment process. As a result of an economic evaluation for the coagulation sedimentation filtration process, the direct coagulation filtration process and the nanobubble multihydrocyclone process, which are the pretreatment processes for seawater desalination, the production cost per ton were 4,850 Won/m<sup>3</sup>, 4,528 Won/m<sup>3</sup>, and 3,821 Won/m<sup>3</sup>, respectively. Therefore, it was found that the nanobubble multi-hydrocyclone pretreatment process reduces operating costs by about 21 % compared to other pretreatment processes.
문상욱 ( Sang Wook Mun ),김형영 ( Hyung Young Kim ),김혜영 ( Hye-young Kim ),박희주 ( Hee Ju Park ),김수연 ( Soo Yeon Kim ),신용범 ( Young Beom Shin ),손승국 ( Seung Kook Son ) 대한천식알레르기학회(구 대한알레르기학회) 2016 Allergy Asthma & Respiratory Disease Vol.4 No.3
Purpose: Use of a home mechanical ventilator can shorten the hospitalization duration of children with chronic respiratory failure requiring long-term use of a mechanical ventilator. In this study, the researchers analyzed patients who had used a home mechanical ventilator. Methods: From January 2009 to July 2014, we retrospectively investigated 15 patients under 18 years of age, from 2 hospitals, and their use of home mechanical ventilators. Results: The median age of the patients was 55 months. Ten children were male, and 5 were female. As for the type of mechanical ventilators, 10 used a pressure type, 3 used a volume type, and 2 used a volume-mask type. Analysis of underlying diseases revealed that 10 children had neuromuscular disease, 3 had pulmonary disease, and 2 suffered from hypoxic ischemic encephalopathy. The number of patients who were discharged from the hospital with a home mechanical ventilator was 13. One child died of sepsis irrespective of the mechanical ventilator. Six patients had health insurance. But 9 did not. The group with insurance had the possibility of being discharged earlier than the group without. On readmission, the length of hospitalization was also reduced in patients who had used a home mechanical ventilator. Conclusion: Use of a mechanical ventilator at home is helpful in patients who need long-term use of a mechanical ventilator due to neuromuscular disease, chronic pulmonary diseases, and accidents because complications are rare and insurance coverage is feasible. (Allergy Asthma Respir Dis 2016;4:195-198)