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민경진,윤혜진,Atsushi Matsuura,김용환,이형호 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.4
L-pipecolic acid is a non-protein amino acid commonly found in plants, animals, and microorganisms. It is a well-known precursor to numerous microbial secondary metabolites and pharmaceuticals, including anticancer agents, immunosuppressants, and several antibiotics. Lysine cyclodeaminase (LCD) catalyzes β-deamination of L-lysine into L-pipecolic acid using β-nicotinamide adenine dinucleotide as a cofactor. Expression of a human homolog of LCD, μ-crystallin, is elevated in prostate cancer patients. To understand the structural features and catalytic mechanisms of LCD, we determined the crystal structures of Streptomyces pristinaespiralis LCD (SpLCD) in (i) a binary complex with NAD+, (ii) a ternary complex with NAD+ and L-pipecolic acid, (iii) a ternary complex with NAD+ and L-proline, and (iv) a ternary complex with NAD+ and L-2,4-diamino butyric acid. The overall structure of SpLCD was similar to that of ornithine cyclodeaminase from Pseudomonas putida . In addition, SpLCD recognized L-lysine, L-ornithine, and L-2,4-diamino butyric acid despite differences in the active site, including differences in hydrogen bonding by Asp236, which corresponds with Asp228 from Pseudomonas putida ornithine cyclodeaminase. The substrate binding pocket of SpLCD allowed substrates smaller than lysine to bind, thus enabling binding to ornithine and L-2,4-diamino butyric acid. Our structural and biochemical data facilitate a detailed understanding of substrate and product recognition, thus providing evidence for a reaction mechanism for SpLCD. The proposed mechanism is unusual in that NAD+ is initially converted into NADH and then reverted back into NAD+ at a late stage of the reaction.
Invasive Squamous cell carcinoma of Vagina with Cerebellar Metastasis
민경진,오영택,소경아,이상훈,김재원,홍진화,송재윤,이낙우,이재관 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-
Primary vaginal cancer is an uncommon tumor, representing only 1% to 2% of malignant neoplasms of the female genital tract. Due to early diagnosis and technical advances of brachytherapy in radiotherapy, the outcome for patients has improved over the past several decades. Distant metastases occur most commonly to lungs and liver. We describe a case of a 72-year-old woman with an invasive vaginal squamous carcinoma, who underwent chemo-radiation initially. Initially, there was no evidence of distant metastasis. However, the patient exhibited nausea, vomiting, slurred speech, and dizziness for several days at 1-year follow-up. Brain MRI was performed to check the lesion, and the suspected metastases were found in right cerebellum. Multiple lung metastases were found in chest x-ray. Conservative management was executed due to the patient`s poor general condition, such as Karnofsky performance status scale (KPS) 50, and refusal to treatment. Cerebellar metastasis should be considered in patients with neurological symptoms despite its rareness in vaginal carcinoma. Patients with multiple brain metastases and poor performance status as in our patient may not benefit from any type of therapy. The treatment plan for CNS metastases should be made on an individual basis, with careful consideration of the ultimate purpose of the treatment.
민경진,Kyung-Yoon Kwon,윤기선 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.1
This study investigated the antimicrobial effects of garlic, potassium lactate and sodium diacetate (PL/SDA) mixture, ε-polylysine, and potassium sorbate (PS) alone or in combinations of them on the growth of foodborne pathogens and production of staphylococcal enterotoxin A (SEA) in ready-to-eat (RTE) pyeonyuk at various temperatures. Lag times (LTs) of Salmonella Typhimurium were longer than those of Staphyloccus aureus at all tested temperatures, regardless of antimicrobial agent. The growth of S. Typhimurium and S. aureus were not observed in pyeonyuk made with 3% PL/SDA mixture or 1.5% ε-polylysine at 10°C. The 0.2% of PS, which is the permitted level for meat products, did not inhibit the growth of S. Typhimurium and S. aureus. Although growth of S. aureus in RTE pyeonyuk with 3% PL/SDA mixture and 1.5% ε-polylysine was observed, SEA was not produced at 17, 24, and 30℃, suggesting the application of PL/SDA mixture or ε-polylysine to reduce the risk related to the consumption of pyeonyuk.