Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

폴리에스테르 섬유 고속방사중 필라멘트의 냉각과정 분석

윤문구,박준원,이승윤 건국대학교 산업기술연구원 1998 건국기술연구논문지 Vol.23 No.-

High speed fiber spinning of polyethylene terephthalate(PET) was simulated at take-up speed of 6000m/min utilizing generalized steady state governing equations of melt spinning and some other assumed relationship for structure formation. In the present paper, the effect of changes of quench air temperature on the spinning line were mainly discussed. Distance of necking point and stress from the spinneret was elongated when higher quench air temperature was applied. Changes of necking point and stress may affect the development of PET fiber structure in the high speed threadline.

시간 경과에 따른 카르복시헤모글로빈 농도 변화에 관한 연구

이윤성,김준호,이정빈 大韓法醫學會 1989 대한법의학회지 Vol.13 No.1

The diagnosis of carbon monoxide (CO) poisoning in clinics and forensic practice largely rests on the determination of carboxyhemoglobin (HbCO) in blood. It is generally known that HbCO remains unchanged gor a couple of days, and such a delay in determination of HbCO may be acceptable. However, there were some incidences of prolonged delay because of initial lack of concern to CO poisoning and other limitations on postmortem examination. The question may be raised on that the prolonged delay would be acceptable for the diagnosis of original CO poisoning. The study was preformed to obtain the changes of HbCO concentration in vitro. Fifteen rabbits were intoxicated with CO in the system which was made on purpose. Initial levels of hemoglobin (Hb) and HbCO were determined by CO-Oximeter (IL 182). And the samples were stored in the refrigerator and in room temperature for 1 and 2 months to check same parameters. The HbCO decreased 5.85±8.37% at one month in room temperature (p<0.01). Meanwhile, after 1 month storage, the HbCO levels of the bloods stored in room temperature were lower as much 4.37±8.24% than those in the refrigerator. The results indicates that the diagnosis of CO poisoning could be obtained after 2 months or later in refrigerated blood, and 1 month after storage in room temperature. It is presumed that the low levels of HbCO in stored blood are partly because of the decreased combining capacity of hemoglobin to CO, which is affected by temperature. And, if the blood is determined for toxic level of CO poisoning after some delay, it should be considered that there is a presumable decrease in HbCO concentration according to the storage temperature.

위험도 정보 활용을 위한 화재 PSA 방법론 개선 연구 현황

이윤환,양준언 한국화재소방학회 2003 한국화재소방학회논문지 Vol.17 No.1

본 논문에서는 현재 세계적으로 사용되고 있는 화재 PSA 방법론을 살펴보고 화재방호를 목적으로 위험도 정보를 이용할 시 요구되는 방법론의 개선 사항을 파악하였다. 화재 PSA의 결과 및 이를 통한 통찰(insight)은 의사 결정을 하는데 완전한 기술적인 근거라 하기 보다는 위험도 정보를 활용한 의사 결정 과정의 일부로서 사용될 수 있으며, 화재 PSA 결과의 적용 분야와 적용 수준은 PSA에서 사용된 모델의 정확성과 타당성에 좌우된다고 할 수 있다. 따라서 화재 PSA의 유용성은 많은 분야의 연구와 개발을 통해 현재 기술 수준 증진에 따라 확대될 것이며, 그에 따라 더 일관성 있는 결과를 낳게 될 것으로 판단한다. In this paper, many vulnerable areas of the present fire PSA methodology were revealed to apply risk-informed fire protection to nuclear power plants. The results and insights from the fire PSA should be used as part of a risk-informed decision making process rather than the complete technical basis for decision making. The degree of support and scope of applications is dependent on the accuracy and validity of the model used in the fire PSA. Accordingly, the usefulness of the fire PSA will increase as ongoing research and development efforts lead to improvements in the state of the art technology, and as improvements in implementation of the state of the art technology lead to more consistent results.

알레르기 환자에서 특이알레르겐 항체 양성율과 관련요인

이선화,남해선,이성수,안현철,황규윤,박준수,,박춘식 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

Recently, allergic diseases are very prevalent in Korea, resulting in physical and economic losses during the life. It is well-known that the principle therapy is identification, eradication, and desensitization of allergens. There are many allergic diagnostic tools for identification of specific-allergens. However, none of them is satisfied with its efficiency due to lack of validity. Recently MAST-CLA has been widely used in the allergic clinical fields because of its simplicity and simultaneous identification of allergens. This study was designed to determine the positivity of allergen-specific antibodies using MAST-CLA in patients with allergic diseases, and evaluate the efficacy for allergen detection, compared to results of skin prick test. During June 2000 - September 2001, 325 study subjects visited Soonchunhyang University Chunan Hospital for the evaluation of allergic problems. Among them, 289 subjects were diagnosed with allergic disease and included for the study. Thirty four subjects visited children's allergic unit were evaluated specific allergens using a skin prick test at the same time. Our result as follows; 1. The positive rates of allergen-specific antibodies and total IgE were 51.2% and 73.4%, retrospectively. 2. There was significant difference in positive rate in age group, showing highest in teen ages(73.6%), but insignificant difference in sex in allergen-specific antibodies. 3. The positive rate was 60.4% in allergic rhinitis, 51.3% in allergic dermatitis, 46.9% in bronchial asthma, and 39.1% in urticaria in order(P=0.073). 4. Significant difference in month or season was observed, with highest rate in April(78.1%) and May(72.1%). The highest season was in spring(62.7%), and then summer(52.8%), fall(50.3%), and winter(41.4%) in order (P=0.04). 5. The three highest allergen-specific antibodies were D. pteronyssinus(39.3%), D. farinae(38.3%), and house dust(24.3%) in Korean inhalant pannel, However, in food pannel types of allergen-specific antibody were different as D. farinae(18.5%), Alternaria(18.5%), house dust(17.3%). 6. There were significant agreements between MAST-CLA and Skin prick test (D. farianae Kappa=0.636;D. pteronyssinus Kappa=0.78, both P<0.001) In conclusion, MAST-CLA could detect allergen-specific antibodies in patients with allergic diseases as a simple and valid tool of screening of allergy. Especially, it could be a useful measure of multiple allergens simultaneously in children However, more detailed allergens and valid assay for unmeasured allergens should be identified for Korean-specific allergens.

소뇌 혈관아세포종 출혈에 의한 돌연사 부검 1예

이윤성,이숭덕,권오준 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

A rare case of fatal intratumoral hemorrhage in a cerebellar hemangioblastoma is described. A 320year-old soldier was presented with loss of consciousness of acute onset, followed by rapidly progressing coma. At autopsy it was shown that the brain was edematous and covered with basal subarachnoid hemorrhage, and well circumscribed cystic tumor was noted in cerebellum. The tumor involved the cerebellar vermis and the right hemisphere, protruding upward from the superior surface of the cerebellum. The cystic portion was filled with fresh blood clot. Histologic study confirmed the diagnosis of hemangioblastoma showing typical two components of large polygonal stromal cell and anastomosing capillary network.

地域間 情報隔差에 관한 硏究 : 서울과 濟州를 中心으로

李聲,裵聖義,尹晙相 公州大學校 産業開發硏究所 1993 産業開發硏究 Vol.1 No.-

The objectives of this study were to identify the regional information gap between seoul and Cheju region, and to present the direction for narrowing the regional information gap. Data were collected mainly by using available data and questionnaire. The numbers of questionnaire subject were 153 and 198 sampled from parents of one middle school respectably in Seoul and Cheju. The number of variables for comparison of information gap between two regions were 14. The major findings of this study were as follows; When the values of each variable of Seoul were 100, the values of each variable of Cheju were as follows; 1) The number of companies per 100,000 populations dealing with Computer Hard Ware and Soft Ware was ratewise 100 in Seoul 2.3 in Cheju, 2) the number of the enterprises per 100,000 populations engaged in information industry was ratewise 100 in Seoul 3.2 in Cheju, 3) the number of HITEL affiliaters and equipment was ratewise 100 in Seoul 25.1 in Cheju, 4) the number of postal matter receipients was ratewise 100 in Seoul 28.4 in Cheju, 5) the number of international communication use was ratewise 100 in Seoul 32.7 in Cheju, 6) the number of agencies per 100,000 populations in liason with information industry was ratewise 100 in Seoul 40.0 in Cheju, 7) the number of books in libraries per 100,000 populations was ratewise 100 in Seoul 63.6 in Cheju, 8) the status of PC-serve use was ratewise 100 in Seoul 74.9 in Cheju, 9) the amount of time in listening to the radio was ratewise 100 in Seoul 82.4 in Cheju, 10) the rate of taking in magazine was ratewise 100 in Seoul 87.5 in Cheju, 11) the population of telephone was ratewise 100 in Seoul 87.8 in Cheju, 12) the amount of time in watching TV was ratewise 100 in Seoul 88.2 in Cheju, 13) the amount of time in reading books and magazines was ratewise 100 in Seoul 89.8 in Cheju, 14) the rate of taking in newspaper which was higher than the rate of Seoul was 101.4 and, 15) the means of these variables which mean the information gap between Seoul and Cheju was ratewise 100 in Seoul 57.7 in Cheju, These direction presented here for narrowing the regional Information Gap Between are : (1) inducement of information industries and companie to remote area by giving prior administrative benefits and other services, (2) strengthened adults and continuing education program related to information conscience and technology, and (3) establishment of department of information science and technology at new or existing institutes of higher leaping in local area.

백서의 실험적 치아 이동시 치주조직내 성장이자 발현에 관한 면역조직화학적 연구

이준형,김상철,국윤아 대한치과교정학회 1995 대한치과교정학회지 Vol.25 No.1

교정력에 의한 치아이동시 치주조직의 조직변화를 알아보고 성장인자중의 하나인 Epidemal Growth Factor(EGF)의 시간 경과에 따른 발현정도 및 분포 변화를 알아 보고자, Sprague-Dawley계 백서 23마리를 대조군(3마리)과 실험군(20마리)으로 나누었으며, 실험군은 교정력(75g)을 가한 후 12시간, 1일, 4일, 7일, 14일이 경과한 후 각각 4마리씩 희생시켜, EGF의 발현 분포와 조직학적 변화를 면역조직화학적 및 조직병리학적으로 관찰한바 다음과 같은 결과를 얻었다. 1. 교정력을 가한 후 14일 까지, 견인측의 치주인대 섬유는 신장되었고, 압박측의 치주인대 섬유는 압축되었으며 치주인대섬유 배열의 완전회복은 일어나지 않았다. 2. 대조군의 EGF 발현은 구강상피, 전상아질, 치수와 치주인대내의 혈관에서 진하게 발현되었으나, 파골세포 및 골아세포에서는 미약한 염색상을 보였다. 3. 교정력을 가한지 12시간, 1일, 4일, 7일째 실험군의 치주조직에서의 EGF의 발현은 견인측과 압박측의 차이가 없이, 경미한 미만성의 양성반응을 보였다. 4. 교정력을 가한지 14일째 치주조직에서 EGF의 발현이 현저히 증가되었으며, 압박측보다 견인측이 더 많이 발현되었으며, 치경부 쪽보다 치근단 쪽에서 더 많았다. This study was designed to evaluate the expression of growth factor in periodontal tissue during the experimental movement of rat incisors by LSAB(Labelled strepatavidine Biotin) immunohistochemical staining for EGF(Epidermal growth factor). 23 Sprague-Dawley rats were divided into a control group(3rats0 and experimental groups(20rats), where a force(75g) from helical springs across the maxillary incisors was applied. Experimental groups were sacrificed at 12 hours, 1, 4, 7 and 14 days, after force application, respectively. And Tissue slides of control and experimental groups were studied immunohistochemically and histologically. The results were as follows : 1. In 14days after force application, periodontal fibers were strectched on the tension side, and compressed in pressure side of all experimental groups, and the arrangement of periodontal fibers was not recovered yet. 2. The degree of EGF expression in control group was strongly positive in the oral epithelium, predentin, capillaries in pulp and periodontal spaces. But osteoblasts and osteoclasts were stained mildly positive. 3. EGF expression was mild and diffuse in 12 hours, 1, 4 and 7 days of experimental groups and was not significantly different between the tension and pressure sides. 4. The degree of EGF expression in the 14-day experimental group was higher than any other group. And the tension side showed a more positive EGF expression than the pressure side. The apical area revealed a more positive EGF expression than the cervical area.