Apoptosis and necroptosis‑inducing effects of arctigenin on nasal septum carcinoma RPMI‑2650 cells in 2D and 3D culture

Yoon‑Jin Lee,Kwan‑Sik Park,Byoung Joon Baek,Kyong‑Ae Lee,Sang‑Han Lee 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.1

Backgrounds Arctigenin derived from the seeds of Arctium lappa Linnaeus is known as an anticancer drug candidate by targeting various pathways involved in anticancer therapy. Methods Using 2D monolayer and 3D spheroid culture systems in nasal septum carcinoma RPMI-2650 cells, the effects of arctigenin and dexamethasone on cell viability, ROS levels, ATP level, mitochondrial function, apoptosis and necroptosis were examined. Results The combination treatment of both compounds induced strong cytotoxicity, accompanied by increases of sub-G0/ G1 peak, annexin V-PE-positive cells, and ROS levels, loss of mitochondrial membrane potential, and decrease of cellular ATP content. These changes were observed as simultaneous induction of DNA damage, apoptosis, and necroptosis. A series of changes by arctigenin and dexamethasone were efficiently restored by decreasing ROS levels or supplementing ATP. Treatment of 3D spheroids with arctigenin and dexamethasone decreased cell viability in the spheroids, but it was slightly resistant than cells under 2D conditions. In addition, this phenomenon was accompanied by an increase in mediators for both apoptosis and necroptosis. Conclusion Results of this study suggest that the apoptosis and necroptosis-inducing effects of arctigenin are associated with ATP depletion due to oxidative mitochondrial dysfunction.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

체력단련이 숨참기동안 및 회복기의 혈압과 심박동수에 미치는 영향

이영만,윤봉영,윤성수,김준곤,이상렬,김성호,배계숙 최신의학사 1984 最新醫學 Vol.27 No.11

위험도 정보 활용을 위한 화재 PSA 방법론 개선 연구 현황

이윤환,양준언 한국화재소방학회 2003 한국화재소방학회논문지 Vol.17 No.1

본 논문에서는 현재 세계적으로 사용되고 있는 화재 PSA 방법론을 살펴보고 화재방호를 목적으로 위험도 정보를 이용할 시 요구되는 방법론의 개선 사항을 파악하였다. 화재 PSA의 결과 및 이를 통한 통찰(insight)은 의사 결정을 하는데 완전한 기술적인 근거라 하기 보다는 위험도 정보를 활용한 의사 결정 과정의 일부로서 사용될 수 있으며, 화재 PSA 결과의 적용 분야와 적용 수준은 PSA에서 사용된 모델의 정확성과 타당성에 좌우된다고 할 수 있다. 따라서 화재 PSA의 유용성은 많은 분야의 연구와 개발을 통해 현재 기술 수준 증진에 따라 확대될 것이며, 그에 따라 더 일관성 있는 결과를 낳게 될 것으로 판단한다. In this paper, many vulnerable areas of the present fire PSA methodology were revealed to apply risk-informed fire protection to nuclear power plants. The results and insights from the fire PSA should be used as part of a risk-informed decision making process rather than the complete technical basis for decision making. The degree of support and scope of applications is dependent on the accuracy and validity of the model used in the fire PSA. Accordingly, the usefulness of the fire PSA will increase as ongoing research and development efforts lead to improvements in the state of the art technology, and as improvements in implementation of the state of the art technology lead to more consistent results.

원자력발전소 증기발생기의 저출력 수위조절의 자동화

이윤준 濟州大學校 工科大學 産業技術硏究所 1991 産業技術硏究報告 Vol.2 No.-

The steam generator water level is difficult to control at low power due to its reversed responses to the feedwater flow, which are well known as the shrink and swell phenomena. With regard to this problem a new control scheme has been studied by which the level transients could be kept within permissible ranges at low power. The relations between the various input conditions to steam generator and the level transients have been examined to be expressed in the form of process transfer functions. Analog filters have been incorporated to the process with proper control constants. This control scheme allows the prediction of level variation together with the corresponding feedwater rate and results in milder transients with good stabilities.

3D-CT를 이용한 골격성 III급 개방교합자의 악교정 수술 전, 후 설골 및 상기도의 변화

이윤섭,백형선,이기준,유형석 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.2

본 연구는 골격성 III급 개방교합자의 악교정 수술 전, 후 설골과 상기도의 변화를 3D-CT를 이용하여 관찰하고 이를 정상교합자와 비교하기 위하여 시행하였다. 전치부 개방교합을 동반한 골격성 III급 부정교합으로 진단되어 악교정 수술을 받은 환자 중 12명을 선정 후, 3D-CT를 통해 설골과 상기도의 3차원 입체영상을 분석하여, 악교정 수술 전, 후의 설골의 위치 변화 및 상기도의 부피 변화를 살펴보고, 이의 결과를 정상교합자 10명과 비교하였다. 설골은 골격성 III급 개방교합군에서 악교정 수술 전에 정상교합군의 설골에 비해 전방에 위치하고 있었고 악교정 수술 후 설골은 후, 상방으로 변화하였으나 유의성은 관찰되지 않았다. Hyoid plane과 mandibular plane이 이루는 각은 악교정 수술 전의 골격성 III급 개방교합군이 정상교합군보다 큰 값을 나타내었고, 악교정 수술 후에는 그 차이가 더욱 증가하였다. 골격성 III급 개방교합군의 상기도 부피는 정상교합자보다 작으며 이는, 악교정수술 후에 더욱 감소하였다. 3차원 영상 분석 결과, 골격성 III급 개방교합자의 상기도는 정상교합자에 비해 좁으며, 하악골 후퇴술 이후 더욱 감소하기 때문에 이는 수술적 개선의 안정성에 영향을 줄 수 있음을 알 수 있었다. Objective: The purpose of this study was to investigate the structural changes of the hyoid bone and upper airway after orthognathic surgery for skeletal class III anterior open bite patients, and make comparisons with normal occlusion. Methods: Pre- and post-operative computed tomography (CT) examinations were performed on 12 skeletal class III anterior open bite patients who were treated with mandibular setback osteotomy. Using the V-works 4.0 program, 3-dimensional images of the total skull, mandible, hyoid bone, and upper airway were evaluated. Results: In the Class III openbite group, the hyoid bone were all positioned anteriorly, compared to the Normal group (p < 0.05). The angle between the hyoid plane and mandibular plane in the Class III openbite group before surgery was greater than in the Normal group (p < 0.05), and the difference increased after surgery (p < 0.01). In the Class III openbite group, the volume of the upper airway decreased after surgery (p < 0.001) and the volume of the upper airway was smaller than the Normal group before and after surgery (p < 0.001). Conclusions: The narrow upper airway space in skeletal Class III openbite patients decreased after mandibular setback osteotomy. This may affect the post-surgical stability.

The Dynamic Controller Design of the Nuclear Steam Generator Water Level Control System

이윤준 濟州大學校 産業技術硏究所 1997 산업기술연구소논문집 Vol.8 No.1

The steam generator feedwater and level control system is designed by two steps of the feedwater control design and the feedback loop controller design. The feedwater servo system is designed by the optimal LQR/LQG approach and then is modified by the LTR method to recover the robustness. The plant characteristics are subject to change with the power variation and tnese dynamic properties are considered in the design of the feedback controller. All the designs are made in the continuous domain and are digitalized by applying the proper sampling period. The system is simulated for the two cases of power increase and decrease. From the results of simulation, it is found that the controller constants would rather be invariable during the power increase, while for the case of power decrease they should be changed with the power variation to keep the system stability.

地域間 情報隔差에 관한 硏究 : 서울과 濟州를 中心으로

李聲,裵聖義,尹晙相 公州大學校 産業開發硏究所 1993 産業開發硏究 Vol.1 No.-

The objectives of this study were to identify the regional information gap between seoul and Cheju region, and to present the direction for narrowing the regional information gap. Data were collected mainly by using available data and questionnaire. The numbers of questionnaire subject were 153 and 198 sampled from parents of one middle school respectably in Seoul and Cheju. The number of variables for comparison of information gap between two regions were 14. The major findings of this study were as follows; When the values of each variable of Seoul were 100, the values of each variable of Cheju were as follows; 1) The number of companies per 100,000 populations dealing with Computer Hard Ware and Soft Ware was ratewise 100 in Seoul 2.3 in Cheju, 2) the number of the enterprises per 100,000 populations engaged in information industry was ratewise 100 in Seoul 3.2 in Cheju, 3) the number of HITEL affiliaters and equipment was ratewise 100 in Seoul 25.1 in Cheju, 4) the number of postal matter receipients was ratewise 100 in Seoul 28.4 in Cheju, 5) the number of international communication use was ratewise 100 in Seoul 32.7 in Cheju, 6) the number of agencies per 100,000 populations in liason with information industry was ratewise 100 in Seoul 40.0 in Cheju, 7) the number of books in libraries per 100,000 populations was ratewise 100 in Seoul 63.6 in Cheju, 8) the status of PC-serve use was ratewise 100 in Seoul 74.9 in Cheju, 9) the amount of time in listening to the radio was ratewise 100 in Seoul 82.4 in Cheju, 10) the rate of taking in magazine was ratewise 100 in Seoul 87.5 in Cheju, 11) the population of telephone was ratewise 100 in Seoul 87.8 in Cheju, 12) the amount of time in watching TV was ratewise 100 in Seoul 88.2 in Cheju, 13) the amount of time in reading books and magazines was ratewise 100 in Seoul 89.8 in Cheju, 14) the rate of taking in newspaper which was higher than the rate of Seoul was 101.4 and, 15) the means of these variables which mean the information gap between Seoul and Cheju was ratewise 100 in Seoul 57.7 in Cheju, These direction presented here for narrowing the regional Information Gap Between are : (1) inducement of information industries and companie to remote area by giving prior administrative benefits and other services, (2) strengthened adults and continuing education program related to information conscience and technology, and (3) establishment of department of information science and technology at new or existing institutes of higher leaping in local area.

알레르기 환자에서 특이알레르겐 항체 양성율과 관련요인

이선화,남해선,이성수,안현철,황규윤,박준수,,박춘식 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

Recently, allergic diseases are very prevalent in Korea, resulting in physical and economic losses during the life. It is well-known that the principle therapy is identification, eradication, and desensitization of allergens. There are many allergic diagnostic tools for identification of specific-allergens. However, none of them is satisfied with its efficiency due to lack of validity. Recently MAST-CLA has been widely used in the allergic clinical fields because of its simplicity and simultaneous identification of allergens. This study was designed to determine the positivity of allergen-specific antibodies using MAST-CLA in patients with allergic diseases, and evaluate the efficacy for allergen detection, compared to results of skin prick test. During June 2000 - September 2001, 325 study subjects visited Soonchunhyang University Chunan Hospital for the evaluation of allergic problems. Among them, 289 subjects were diagnosed with allergic disease and included for the study. Thirty four subjects visited children's allergic unit were evaluated specific allergens using a skin prick test at the same time. Our result as follows; 1. The positive rates of allergen-specific antibodies and total IgE were 51.2% and 73.4%, retrospectively. 2. There was significant difference in positive rate in age group, showing highest in teen ages(73.6%), but insignificant difference in sex in allergen-specific antibodies. 3. The positive rate was 60.4% in allergic rhinitis, 51.3% in allergic dermatitis, 46.9% in bronchial asthma, and 39.1% in urticaria in order(P=0.073). 4. Significant difference in month or season was observed, with highest rate in April(78.1%) and May(72.1%). The highest season was in spring(62.7%), and then summer(52.8%), fall(50.3%), and winter(41.4%) in order (P=0.04). 5. The three highest allergen-specific antibodies were D. pteronyssinus(39.3%), D. farinae(38.3%), and house dust(24.3%) in Korean inhalant pannel, However, in food pannel types of allergen-specific antibody were different as D. farinae(18.5%), Alternaria(18.5%), house dust(17.3%). 6. There were significant agreements between MAST-CLA and Skin prick test (D. farianae Kappa=0.636;D. pteronyssinus Kappa=0.78, both P<0.001) In conclusion, MAST-CLA could detect allergen-specific antibodies in patients with allergic diseases as a simple and valid tool of screening of allergy. Especially, it could be a useful measure of multiple allergens simultaneously in children However, more detailed allergens and valid assay for unmeasured allergens should be identified for Korean-specific allergens.