서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

고령 환자에서의 간 절제술

김병철,서거정,김경종,장정환,김성환 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Hepatic resection for removal of lesion of the liver may be necessary for a wide variety of conditions. Partial hepatectomy for liver disease have become more common This reports describes a review of our experience for hepatic resection and an analysis of potential risk factors affecting the morbidity and the mortality in a hepatectomy methods : Between Jan 1,997 and Mar.2,001, we performed 112 cases of partial hepatectomy and retrospectively analyzed clinicopathological features of the presented. cases Results : The most common disease was intrahepatic duct stone(31.8%). The mean operative time was 324 minutes. The overall In-hospital mortailty an morbidity rates were 6.8%(3/44) and 59%(27/44), respectively, Various posmiddleerative complications developed, including 14 cardiopulmonary complications(53.8%), 8 wound Infections(30.7%), 2 Bile leakage(7%), 1 intraabdominal abscess(3.8%), 1 hepatic failure(38%), 1 posmiddleerative bleeding(3.8%). The old had more cardiopulmonary complications. The significant risk factors for perioperative mortality were high preoperative serum bilirubin level(above 2.0mg/dl), size of resection, massive operative bleeding(above 2,000cc), concomitant diseases, but cirrhosis was not a significant risk factor. Comparison of young age patients, the old had more complications, but it was not significantly Conclusion : Although the old patients had more cardiopulmonary complications but their operative mortality were not much different than younger patients significantly. Hepatic resection can be performed in over 60 years old with acceptable morbidity and mortality rates.

두부 손상이 골절 칭 촉진 시동물 모델 및 유전자 발현 분석

김경희,김학선,문성환,김향,권언해,이환모,박희완,한수봉,문은수 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

Application of heterogeneous RNA probes labeled immuno-fluorescent agent DIG for the screening a noble gene in cucumber

Kim,Dai-Hee,Kim,Byung-Oh,Park,Gyu-Hwan,Takahashi,Hideyuki,Kim,Kyung-Min 한국자원식물학회 2004 Plant Resources Vol.7 No.2

We tried to isolating a noble gene from cucumber library with heterogeneouse RNA probe labeled DIG of Arabidopsis PIN3 gene. Two kinds of RNA probes which had no significant homology each others, were designed from the 5 - and 3 - prime nucleotides of the AtPIN3 gene. In the first and second screenings of the cDNA library of cucumber with the probes, two positive clones were identified with specific duplicate signals. However, we isolated cDNA fragments homologous with putative nucleases from Nicotiana, Arabidopsis, Cordialis, and Oryza sativa, there was no significant homology with any other PIN family genes.

An Improved Speech Processing Strategy for Cochlear Implants Based on an Active Nonlinear Filterbank Model of the Biological Cochlea

Kim $^$, Kyung Hwan,Choi, Sung Jin,Kim, Jin Ho,Kim, Doo Hee IEEE 2009 IEEE Transactions on Biomedical Engineering Vol.56 No.3

<P>The purpose of this study was to improve the speech processing strategy for cochlear implants (CIs) based on a nonlinear time-varying filter model of a biological cochlea. The level-dependent frequency response characteristic of the basilar membrane is known to produce robust formant representation and speech perception in noise. A dual resonance nonlinear (DRNL) model was adopted because it is simpler than other adaptive nonlinear models of the basilar membrane and can be readily incorporated into the CI speech processor. Spectral analysis showed that formant information is more saliently represented at the output of the proposed CI speech processor compared to the conventional strategy in noisy conditions. Acoustic simulation and hearing experiments showed that the DRNL-based nonlinear strategy improves speech performance in a speech-spectrum-shaped noise.</P>

비위관 삽입시 국소마취의 효과

김태경,정순미,김인병,김경환 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose: This study was conducted to assess the effect of sprayed 10% xylocaine as a local anesthesia in reducing the pain associated with nasogastric tube(NGT) placement Method: This was a prospective, randomized, case-controlled trial conducted in the emergency department of a university teaching hopital. Study participants were alert patients requiring NGT placement for diagnostic or therapeutic purposes. Patients having NGT placement were randomized to receive either a 10% xylocaine solution or nothing sprayed in the nasopharynx and oropharynx, After 5 minutes, the distal 5 cm of the NGT were lubricated with a liberal amount of sterile, water-soluble, and non-anesthesient jelly before placement. After each procedure, the patient recorded assessment of subjective pain on a 100-mm visual analog scale(VAS). Result: A total of 71 patients were evaluated in the study with 32 in the case group and 39 in the control group. The mean VAS value of the case group was 38.8 mm(95% confidence interval[CI] 2.4 to 51.3); that of control group was 63.31 mm. The mean difference between groups was 24.40 mm(95% CI, 26.9 to 75.9). A statistically significant difference was achieved between the case and the control groups(p<0.01). Conclusion: Sprayed nasopharyngeal and oropharyngeal 10% xylocaine results in a statistically significant reduction in pain during NGT placement.

항암제 처리한 백혈병 세포주에서의 Apoptosis 발현

김삼용,윤소현,김현수,김종숙,윤환중,김진경,조덕연 충남대학교 암연구소 1998 癌共同硏究所 硏究誌 Vol.2 No.1

The bcl-2 proto-oncogene encodes a 26 kD protein that promotes cell survival by blocking apoptosis. The bax protein is a member of the bcl-2 familly, now known to form heterodimers with the bcl-2 protein. The ratio of bax to bcl-2 is be critical in determining the fate of the cell in response to stimuli that can induce apoptosis. Extract of Pulsatilla Koreana (SB-31) showed promising antitumor activity in vitro with Topo I inhibitory action. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax were investigated in human leukemic cell lines HL-60, U-937 and CEM-CM3. All anticancer drugs(adriamycin, etoposide, camptothecin, SB-31) induced extensive apoptosis in HL-60, U-937 cells and CEM-CM3 cells. The expression of bcl-2 and bax protein were determined in cell lines by western blotting before and after incubation with anticancer drugs at different time points. 1) In HL-60 or U-937 cell lines, down regulation of bcl-2 and up-regulation of bax were found after incubation with ADR, VP-16 or camptothecin. 2) In HL-60 or U-937 cell lines, no significant change in bcl-2 or bax protein expression resulted after incubation with SB-31. 3) In CEM-CM3 cells, virtually no change was noted in bcl-2/bax expression after incubation with ADR, VP-16, camptothecin or SB-31. It is suggested that different leukemic cell lines use different pathways of apoptosis activation and a given cell may utilize different pathways of apoptosis activation in response to different cytotoxic agents.

급성신부전을 동반한 알콜성 횡문근융해증 2례

김영민,오동렬,이원재,김형국,황두영,이환,최경호,정시경,김세경,김영옥 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Rhabdomyolysis is a biochemical and clinical syndrome caused by lysis of skeletal muscle cell and release of muscle cell contents into the circulation. In addition to traumatic cause, various nontraumatic causes have been reported. Rhabdomyolysis has frequently been associated with seamy aspects of life and society: alcoholic abuse, drug addiction, sadistic drill exercise, war events and attempted suicide. The abuse of alcohol causes many hazards in many organs. One of these is acute alcoholic myopathy that may present as a fulminent syndrome of muscle pain and tenderness associated with rhabdomyolysis and acute renal failure. Because the prognosis of adequately treated rhabdomyolysis is excellent, early recognition and prompt management are essential in emergency situation. We experienced two typical cases of alcohol-induced rhabdomyolysis with acute renal failure and report these cases with literature review.