Tabes Dorsalis의 핵자기공명영상 및 전기생리학적 소견

권기한,김성민,권석범,이병철,조광호,주정희 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3

접착제 미 사용시 치면열구전색제로서의 유동성 복합레진 평가

권호범,김명진,신철환,김지연,박기태 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

본 연구의 목적은 유동성 복합레진을 치면열구전색제로 사용할 수 있는지 여부를 평가하는 것이다. 세 종류의 유동성 복합레진(Filtek Flow, Tetric Flow, Charmfil Flow)과 한 종류의 filled sealant(Ultraseal XT plus)가 사용되었다. 주사전자 현미경을 이용하여 세 종류의 유동성 복합레진 및 filled sealant의 resin tag 형성 양상을 비교하였다. 미세누출 평가를 위해 발거된 사람의 소구치 54개를 각각 18개씩 무작위로 세 군으로 배분하였다. 각 군마다 통상의 filled sealant 와 한 가지씩의 유동성 복합레진을 교합면 열구에 도포하였다. 치아들은 thermocycling(5˚ ± 2℃와 55˚ ± 2℃ 사이에서 30초씩 1200회 시행) 후 48시간 동안 1% methylene blue 용액에 보관하였다. 각각의 치아를 절단하여 미세누출 정도를 관찰하였다. 세 종류의 유동성 복합레진 및 filled sealant는 유사한 resin tag 형성양상을 보였다. 세 종류의 유동성 복합레진은 각각 filled sealant보다 현저히 더 많은 미세누출을 보였다. 세 종류의 유동성 복합레진 간의 미세누출 정도는 유사하였다. 유동성 복합레진은 통상의 filled sealant와 비교해볼 때 보다 많은 미세누출을 야기할 수 있으므로 치면열구전색제로 적절하지 않다. The aims of this study were to determine if flowable composites can be used as pit and fissure sealants without bonding agents. Three flowable composites(Filtek Flow, Tetric Flow, Charmfil Flow) and a filled sealant (Ultraseal XT plus) were used. The pattern of resin tag formation in the four sealant meterials were compared using SEM. For the microleakage assessment, 54 extracted human premolar teeth were randomly divided into 3 groups containing 18 premolars each. In each group, a conventional filled sealant and one of the three flowable composites were applied to occlusal fissures. The teeth were thermocycled(1200 cycles between 5˚± 2℃ and 55˚± 2℃ with a dwell time of 30 seconds) and immersed in a 1% methylene blue solution for 48 hours. Each tooth was sectioned and examined to determine the extent of dye penetration. Three flowable composites and a filled sealant showed a similar resin tag formation pattern. The three flowable composites showed significantly more microleakage in each group than the filled sealant. The level of microleakage was similar in the three flowable composites. Flowable composites are not recommended as pit and fissure sealants because more microleakage can occur even when occlural fissures are mechanically widened.

패밀리레스토랑 종업원의 위생과 청결에 관한 중요도 인식

고호석,김선경,김동기,김범진 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.2

The purpose of this study was to identify factors associated with sanitation and cleanliness regarding the importance of family restaurant employees in the Ulsan and Basin areas. Accordingly, this study conducted a survey questionnaire consisting of 28 measures of food-service as well as major subject descriptors. From the results, the frequency, descriptive, factor, correspondence analysis, major findings and marketing implications could be summarized as follows: First, 6 factors were extracted: cooking and control factor, food and tableware handling factor, personal cleanliness condition factor, purchase and receiving factor, unit food material store factor and Kitchen utensil sanitation factor. KMO and Bartlett'stest statistics showed that the data fitted the factor analysis well. Results of factor analysis, average variance extracted estimates and shared variance showed that the convergent and discriminant validity of 6 factors are supported and Cronhach's alpha showed that the internal consistency of the 6 factors was supported. Second, correspondence analysis indicated that statistically significant relationships existed between some of the family restaurant employee sanitation and cleanliness factors and the family restaurant enterprise.

Systemic Lupus Erythematosus를 동반한 Multiple sclerosis

이지현,이기주,윤성호,부귀범,손현화,박유환,정춘해,김진호,정원영 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

Multiple sclerosis and lupus erythematosus are chronic, potentially disabling diseases of unknown cause. It is a rare occasion that the finding of both diseases occurs in one patient. Because both diseases may affect the central nervous system, it may be difficult at times to differentiate symptoms of lupus erythematosus from those of multiple sclerosis. As far as we know, there was no case report of SLE in multilple sclerosis in Korea. We report a case of multiple sclerosis accompanied by SLE.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

한국인에서 Quadruplex PCR을 이용한 8종류 상염색체 STR 유전좌의 다형성 분석

장영길,김기범,신창호,이숭덕,이윤성,이정빈 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5% polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 085 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65×10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here preyed to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.

대량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별

이숭덕,김기범,이윤성,최영태,신창호,이정빈 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The mitochondrial DNA(mtDNA) is a small extranuclear DNA molecule that has been sequenced in man. the mtDNA has distinct characteristics over nuclear DNA. First, the evolutionary rate of nucleotide substitution appears to be larger compared with the nuclear DNA. Second, mtDNA is maternally inherited, so the determination of the various haplotype is unequivocal and no recombination has to be involved. Third, there is a 1.1 kb long control region, which shows severe polymorphism. These characteristics have been applied for several evolutionary study. Furthermore, they exist in cytoplasm with numerous copy number and their size, 16,569 bp is small relative to nuclear DNA, so they are more resistant to degradation or can be types in samples with only cytoplasm such as hair shaft without hair root. These presented the possibility of mtDNA as a tool in individual identification, especially when the nuclear DNA be unavailable. Actually, polymorphism in control region has been applied to individual identification for the skeletal remain found in Vietnam war. So we decided to apply the polymorphism of mtDNA in control region for the individual identification in case of mass disaster-"Downfall of Sampoong Department". Human remains from total 27 different individuals and 178 control persons were submitted for the individual identification. As the mtDNA comparison could be done through maternal lineage, 12 individual from 9 families from the control group were ruled out, because these families were consisted of father or were seeking for their father. Sequencing for the mtDNA control region was done using ABI automatic sequencer and DyeDeoxy Terminator Cycle Sequencing Kit. For convenience, sequencing reaction was done for two separate control region, region I from 16016-16401 in Anderson sequence, and regionⅡ from 048-388. First, sequences in regionⅡ of all samples and control persons were compared, and 20 samples were ruled out with no identical sequences in control group. Remaining 7 samples showed identical sequences with some of the control persons in three groups, and these identical pairs underwent mtDNA region I study. After region I study all samples except two dropped out in blood relationship. For the confirmation, STR and VNTR study such as MCT118, apoB, YNZ22, vWF, MBP, D21S11, TC-11 were done for remaining the two samples. The result denied the blood relationship between these two samples and any of the control group.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

다량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별 : 삼풍백화점 사건에 대하여

이숭덕,신창호,김기범,최영태,이윤성,이정빈 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

韓國産 夜行性 맵시벌科(벌目: 벌亞目)의 分類學的 硏究

이종욱,정종철,김기범,김정한,배지득,남상호 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.2

영남대 생물학과에 소장되어 있는 표본과 2001년 4월~2002년 6월 사이에 채집한 표본을 대상으로 하여 한국산 야행성 맵시벌의 분류학적 연구와 출현 현황 및 숙주 관계의 분석을 실시하였다. 분류학적 연구 결과 Gauld(1970)가 영국산 야행성 맵시벌을 대상으로 한 연구에서 기록한 Mesochorinae아과, Tryphonin-ae아과, Ctenoplematinae아과, Ophioninae아과의 4아과외에 Metopinae (Metopius속)를 추가하여 한국산 야행성 맵시벌류는 총 5아과 13속을 기록하였다. 또한 한국산 야행성 맵시벌의 출현 현황을 분석한 결과 야행성으로 잘 알려진 Mesochorinae아과의 Mesochorus속, Tryphoninae아과의 Netelia속과 Ophioninae아과가 전체 야행성 맵시벌 증 95%를 차지하였다. 속별 출현현황을 살펴보면 Netelia속이 393개체가 출현하여 가장 많은 수를 나타내었으며 Ophi-on속, Mesochorus속, Enicopilus속도 전체 출현 분류군 중 높은 비중을 차지하였다. 월별 현황을 살펴보면 전반적으로 4월에서 11월 사이에 걸쳐 나타나는데, Op-hioninae아과는 5월과 7월에, Tryphoninae아과는 6월에 집중적인 출현 빈도를 보였다. 숙주의 분류군에 따른 야행성 맵시벌의 기생 양상을 살펴보면 대부분이 나비목을 대상 숙주로 하고 있으며 Mesochorinae아과와 Ctenopelmatinae아과는 벌목에도 많은 종이 기생하는 것으로 나타났다. 특히 Mesochorinae아과의 Cidaphus속은 위생 해충으로 분류되는 기생파리과(Tachinidae)에도 기생하는 것으로 나타나 위생 해충을 구제할 수 있는 천적 분류군으로 연구될 수 있는 가능성을 보였다. 숙주의 식성에 따라 살펴보면 부식성의 먹이를 섭식하는 부식성(saprophagus) 숙주, 곤충의 유충을 섭식하는 식충성(entomophagus)인 숙주가 소수 출현하였고 기생생활(parasite)을 하는 숙주도 다수 출현하였으나 대부분의 야행성 맵시벌의 기생 대상 숙주는 주로 식물을 해하는 식식성 곤충(phytophagus)으로 나타났다. Nocturnal Ichneumonidae, which was called yellow-brown ichneumonoid, included several subfamilies adapted for nocturnal activity. Taxonomic study of nocturnal ichneumonoid has been investigated by Gauld(1975) but examination of Korean nocturnal ichneumonoid has not been provided until now. This present study: the taxonomic revision, Appearance state and Host-parasite relation analysis of nocturnal ichneumonoid, were examined using Korean peninsula specimens. As the result. approximately 13 genera referable to 5 subfamilies appeared regularly in light trap. Genus MetopiudSubfamily Illetopinae) were newly recorded in nocturnal ichneumonoid. Tryphoninae and Ophioninae, which were dominant in appearance taxa! occupied 96% of total numbers of individuals, and majority of them were taken from May to November. Nocturnal ichneumonoid are parasitoids of part of immature holometabolous insects(Coleoptera, Diptera, Hymenoptera, Lepidoptera). Lepidoptera is the most common hosts of nocturnal group except Mesochorinae and Ctenopelmatine. In large number of Mesochorinae are koinobiont hyperparasitoids of ectoparasitic or endoparasitic BraconiddHymenoptera), and, less frequently, of Tachinidae(Diptera1. Also, Ctenopelmatinae are koinobiont endoparasitoids of Symphyta and, rarely, Lepidoptera. Majority of nocturnal ichneumonoid hosts are phytophagous, feeding plant taxa, and many host of Mesochorinae taxa are parastoids, and a few host are saprophagus or entomophagus.