Serratia liquefaciens AL-11이 생산하는 Alkaline Lipase의 특성 및 작용양상

최청,김태완,안봉전,김영활,손준호,김성,최희준 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

본 효소의 최적 반응온도는 약 45℃이고, 최적 pH는 10.0 정도였고, pH 7.0~10.0 범위와 30~50℃의 범위에서 안정하였다. 금속이온중 Mn^2+, Ca^2+ 등에 의하여 활성이 증대되었으나 Fe^2+, Pb^2+와 Zn^2+ 등에 의해서는 효소 활성이 저해되었고, 효소활성 저해제 중 ethylenediaminetetraacetic acid(EDTA)에 의해 강한 저해작용을 나타내어 본 효소는 효소분자 중 금속이온이 관여하는 것으로 추정되었다. 효소반응 처리한 olive oil 가수분해물을 박충크로마토그래피 분석한 결과 Serratia liquefaciens AL-11이 생산하는 지방분해효소는 기질특이성이 비특이적이었으며, sodium cholate, sodium edoxychol-ate, sodium taurocholate 등의 담즙산염에 의해 효소활성이 증대되었다. The optimum temperature and pH for the enzyme activity were 45℃ and 10.0, respectively. The enzyme was stable in a pH range of 5 to 10, and 62% of its activity was lost on heat treatment at 60℃ for 20 min. The activity of the purified enzyme was inhibited by Fe^2+, Zm^2+ and Pb^2+, and slightly activated by Mn^2+ and Ca^2+. γ-Chloromercuribenzoic acid, 2,4-dinitrophenol and H_2O_2 did not show inhibitory effect on the lipolytic activity of the alkaline lipase but ethylenediaminetetraacetic acid inhibited the enzyme activity. This suggested that the enzyme have metal group in its active site. Sodium salts of bile acids stimulated the enzyme activity. Analysis of hydrolyzates of olive oil after the lipase reaction revealed that Serratia liquefaciens AL-11 produced non-specific lipolytic enzyme.

부산 신평·장림공단지역의 대기오염물질 배출 특성

김상헌,정장표,김영태,조성훈 慶星大學校 環境問題硏究所 1995 環境硏報 Vol.5 No.1

Industrial growth and urban gravitation of the population etc. have seriously deteriorated the air quality which has adverse effect on human health and property, and the situation is more serious in industrial complex as well. Therefore, it is necessary to examine the pollutants emissions inventory to establish proper and comprehensive strategies for the reduction of pollutant levels. This study is aimed at investigating the characteristics of the pollutant emissions in the vicinity of Sinpyeung·Jangrim industrial complex in Pusan. The emission quantities of air pollutants were estimated by the grid(1㎞x1㎞), the source classification, and the time on the basis of the type and the amount per month of the fuel. The important results from this study were as followings: The average emission quantity(rate) of SO₂, TSP, NOx, CO and HC showed 1926.74 ton/yr(61.09 g/sec), 367.83 ton/yr(11.66 g/sec), 1440.64 ton/yr(45.68 g/sec), 4698.33 ton/yr(148.98 g/sec) and 447.15 ton/yr(14.18 g/sec), respectively. In case of SO₂and TSP, the emission quantities were majorly contributed by stationary source(point source and area source) whose contribution percentages were calculated as 65.8%, 30.6%. On the other hand, emission quantities of NOx, CO and HC were highly contributed by mobil source(line source) whose contribution percentages were calculated as 59.3%, 84.2% and 88.6%.

경상도 전통 마른 명태 식해의 품질특성

최청,구태호,김성,최희진,성태수 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.3

The quality characteristics of traditional Kyungsangdo Myungtae (Alaska pollack) sikhae including optimal composition of the product were investigated. In the sensory evaluation, the Myungtae sikhae made from Gampo accepted to be best for traditional Kyungsangdo Myungtae sikhae and it is called standard sikhae. The standard sikhae was prepared as follows: dried-Myungtae 200g, cooked rice 230g, red pepper 92g, garlic 112g, ginger 100g, radish 1.6kg, salt 20g, and malt meal 65g. The Myungtae sikhae made from Gampo showed higher values in total sugar, free reducing sugar, and nitrogen compounds than the Myungtae sikhae made from another locations. The sugar of standard sikhae consisted of four kinds including glucose. And, the contents of free amino acid was increased in the order of glutamic acid, alanine, and methionine. The composition of free amino acid in Myungatae sikhae contained 17 kinds, and the contents was increased in order of glutamic acid, aspartic acid, and proline. The organic acid of standard Myungtae sikhae consisted of 7 kinds including lactic during fermentation. The contents of citric acid, succinic acid, and lactic acid gradually increased during fermentation, and especially, increasing of lactic acid remarkably increased than the different organic acid.

海洋微生物에 의한 高濃度鹽分含有 廢水處理의 基礎硏究 (Ⅰ)

尹星閏,河淸根,李泰永,張哲鉉,朴勝祚 동아대학교 환경문제연구소 1989 硏究報告 Vol.12 No.1

This study considered organic matter decomposition by ocenic microbes as researching organic matter decomposition on kinetics which is in salinity wastewater under condition that the salinity contains 18,000㎎/l Cℓ^(-) The results of this study are obtained as following : 1. Oceanic microbe of this study is yeast, R. glutinis var. salinaria of marina of Rhodotorula genuses. 2. When retention time of filter bed in Bio-coal tower is 24 hr, 1/2V_(max) is 33.75㎎/l·hr, When 72hr, 1/2V_(max) is 15㎎/l·hr, When 120hr, 1/2V_(max) is 10㎎/l·hr. 3. The reaction between oceanic microbes and organic matter is said to be first order and when contact time of filter bed is 2.0hr, k is 0.40 day^(-1).

유구치 치관 및 기성금속관의 크기에 관한 계측학적 연구

최지은,정태성,김신 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

치아의 크기는 유전적으로나 비교해부학적, 치과 임상적인 면에서도 중요하다. 치아의 크기는 주로 복합적인 유전에 의해 결정되며, 다른 인종간에 차이가 나므로 인류학적 조사를 하는 데에도 사용된다. 특히 유치의 크기는 치과 임상적인 면에서 중요한데, 교합의 변화와 공간분석에 큰 역할을 한다. 소아치과 임상에서 사용하는 유구치의 수복에 주로 사용되는 금속관은 대부분 기성 제품이므로 수복시에는 치아 크기에 맞추어 선택해 사용한다 유치 치관 크기는 인종에 따른 차이가 많은데 현재 임상에서 사용중인 기성금속관은 한국인 기준으로 만들어진 것이 아니므로,기성 제품을 사용하기 위해서는 한국 아동 유치의 형태학적 특성을 정립하는 것이 우선되어야 한다. 한국 아동의 유구치의 인종적 특성과 좌우 비교,성별을 비교하고,소아치과 임상에서 많이 사용하는 기성 금속관의 크기와의 비교를 위한 기초 자료를 얻기 위하여 부산 ·경남지역 아동 235명의 경석고 모형을 채득하여 유구치의 근원심경과 협설경을 digital calipers로 측정하였다. 또 시판중인 2종의 기성금속관의 근원심경과 협설경을 측정하여 , 모형에서 계측한 유구치의 측정치와 비교하여 다음과 같은 결과를 얻었다. 1. 유구치 치관의 크기에 있어서 좌우 차이는 인정되지 않았으나.모든 유구치에서 남아의 경우가 여아보다 큰 것으로 나타났다(P<0.05) . 2. 회귀분석을 통한 비교결과 상, 하 제1유구치와 그 기성금속관간에는 다른 유구치에 비해 상대적으로 큰 차이를 보였다. Data derived from odontometric studies are useful in many areas. Especially crown diameter of primary teeth is important in not only anthropology but also clinical dentistry. The purposes of this study were to compare diameters of primary molars of Korean children with those of preformed stainless steel crown, and examine racial characteristics of primary molars of Korean children. The mesiodistal and buccolingual diameters of primary molars were measured on dental casts taken from 235 children(male 105, female 131), with digital calipers. And the data were compared with those of preformed stainless steel crowns. The results were as fo11ows ; 1. No significant difference was observed between the right and left members of antimeric teeth. and all primary molars of male were larger than those of female 2. The diameters of primary molars of Korean are smaller than those of Austrailian aborigines and there was significant differance between diameters of primary molars of Korean and those of other populations. 3. There were size differences between diameters of maxillary. mandibular first molar and preformed stainless steel crown than other primary molars, relatively

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

실리카흄을 흔입한 고강도 콘크리트 개발

홍창우,김태경,김경진,김성환,김남윤,심도식,이정호,윤청호,백민경,원치문,박제선,이주형,정경일 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

콘크리트 구조물이 대형화됨에 따라 설계단면이 증대되어 상대적으로 많은 경제적 손실을 부담하게 된다. 따라서 단면을 감소시키면서도 소요의 하중에 안전한 구조물을 건설하기 위해서는 우선적으로 구조물 건설에 기초가 되는 고품질, 고내구성의 고강도 콘크리트 개발이 절실히 요구된다 본 연구에서는 일정 시멘트비 및 혼화재 비율하에서 소요의 워커빌리티가 확보되는 고강도 콘크리트를 시간과 온도의 변화에 따라 증기 양생하여, 압축, 인장, 휨강도 뿐만 아니라 파괴특성을 실험적으로 연구하였다. 시료 제작시 시멘트 입자 사이의 공극 및 불연속 영역을 충전하여 고밀도화하기 위한 콘크리트용 혼화재로 시멘트 비표면적이 상당히 작은 초미립 분말인 실리카흄을 이용하였다. 또한 AE감수제 및 고성능 유동화제를 사용하여 혼화재의 첨가에 의하여 발생될 수 있는 워커빌리티의 감소를 방지하였다. 실험결과에 의하면 일정 양생 지속 시간하에서 온도의 증가에 따라 콘크리트의 압축, 인장 및 휨강도가 전반적으로 증가하였다. 동일하게 일정 온도하에서 양생 지속시간이 커짐에 따라 강도들이 증가됨을 알 수 있었다. 따라서 상대적으로 높은 온도와 긴 지속 양생 조건하에서 파괴에 대한 저항력이 크게 나타났다. The compressible, tensible, and flexibleresistance of the high strength concrete is analyzed by the experimentation in the present study. For the test, we cure several samples with the silica fume as a mixture being become dense the spaces between the particle of cement under the variation of both the temperature and the curing-interval. Then, the superplasticizer and the ezcon are also used to satisfy the required workability for construction. The compressible, tensible, and flexible resistances to a stress are increased as increasing the temperature and the time interval for the curing. Therefore it is concluded that the overal fractural and mechanical properties is improved by mixing the silica fume into the cement.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Association of <i>RANBP1</i> haplotype with smooth pursuit eye movement abnormality

Cheong, Hyun Sub,Park, Byung Lae,Kim, Eun Mi,Park, Chul Soo,Sohn, Jin‐,Wook,Kim, Bong‐,Jo,Kim, Jae Won,Kim, Ki‐,Hoon,Shin, Tae‐,Min,Choi, Ihn‐,Geun,Han, Sang‐,Woo,H Wiley Subscription Services, Inc., A Wiley Company 2011 American Journal of Medical Genetics Part B: Neuro Vol. No.

<P><B>Abstract</B></P><P>Schizophrenia is a multifactorial disorder and smooth pursuit eye movement (SPEM) disturbance is proposed as one of the most consistent neurophysiological endophenotype in schizophrenia. The aim of this study was to examine the genetic association of <I>RANBP1</I> polymorphisms with the risk of schizophrenia and with the risk of SPEM abnormality in schizophrenia patients in a Korean population. Two SNPs of <I>RANBP1</I> were genotyped by TaqMan assay. Their genetic effect of single/haplotype polymorphisms on the risk of schizophrenia and SPEM abnormality from 354 patients and 396 controls were performed using <I>χ</I><SUP>2</SUP> and multiple regression analyses. Although no <I>RANBP1</I> polymorphisms were associated with the risk of schizophrenia, a common haplotype, <I>RANBP1‐ht2</I> (<I>rs2238798G–rs175162T</I>), showed significant association with the risk of SPEM abnormality among schizophrenia patients after multiple correction (<I>P</I><SUP>corr</SUP> = 0.002–0.0003). The results of present study provide the evidence that <I>RANBP1</I> on 22q11.21 locus might be causally related to the SPEM abnormality rather than the development of schizophrenia. © 2010 Wiley‐Liss, Inc.</P>

CLASSIFICATION OF AQUATIC AREAS FOR NATURAL AND MODIFIED RIVERS

Cheong, Tae-Sung,Seo, Il-Won Korea Water Resources Association 2001 Water engineering research Vol.2 No.1

For the design of suitable aquatic habitats and habitat management purposes, sensitive descriptors for aquatic areas were identified and analyzed. The classification system of the aquatic areas were developed for natural streams and modified streams in Korea. Relationships among the descriptors of an aquatic area such as channel width, meander wave length, and arc angle have been defined. The analysis indicates that the total mean sinuosity is 1.25 for the main channels of natural streams, whereas the mean value of the sinuosity of modified streams is 1.14. The mean values of the total area, the width, and the length for the sandbars of natural streams are larger than those of modified streams.