추출조건에 따른 울금의 항산화 및 미백 효과

안봉전,이진영,박태순,편정란,배호정,송미애,백은지,박정미,손준호,이창언,최경임 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.3

화장품에 응용 가능한 소재를 발굴하기 위한 목적으로 추출조건에 따른 울금 (Curcuma longa L.)의 항산화 효과 및 미백 효과를 수행한 결과는 다음과 같다. 1. DPPH 라디칼에 대한 소거능은 시료 농도 1,000 μg/ml에서 초임계 추출 시료는 89.0%의 저해율을 나타내어, 3가지 추출법 중 초임계 추출물이 가장 높은 전자공여능을 나타내었다. 2. 울금 추출물의 SOD 유사활성을 측정한 결과 3가지 추출방법 중 초임계 추출물의 경우 시료 농도 500 μg/ml에서 95.0% 가장 높은 SOD 유사활성을 나타내었다. 3. Xanthine oxidase 저해활성을 측정한 결과, 시료 농도 1,000 μg/ml의 초임계 추출물에서 40%의 저해능을 나타내어 물, 에탄올, 1,3-butylene glycol 추출물에 비해 높은 효과를 나타내었다. 4. 울금 추출물의 tyrosinase 저해 활성을 측정한 결과 에탄올 및 초임계 추출물의 경우 1,000 μg/ml의 농도에서 각각 82.0, 89.0%로 높은 tyrosinase 저해 활성을 나타내었다. 이상의 실험 결과를 종합해 볼 때, 울금을 이용한 4가지 추출법 중 초임계추출법이 가장 높은 항산화 효과 및 미백효과를 나타내어 화장품의 소재로서 응용이 가능할 것으로 사료된다. Curcuma longa L. of Zingiberaceae family, has been reported to have radical scavenging activity and antiinflammatory effect. On these facts, biological activity and whitening effect were examined to evaluate a bioactivity of the extracts of Curcumma longa L. by extraction conditions as a possible pharmaceutical material. When compared extraction methods, supercritical extraction was the best showing various biological activities and whitening effect. The highest electron donating ability (89% at 1,000μg/ml) were detected and SOD-like activity was inhibited up to 95% at 500μg/ml in supercritical extraction. To evaluate the whitening effect, tyrosinase inhibitory activity was conducted 89% of tyrosinase inhibitory effect examined at 1,000 μg/ml. From these results, we suggest that the supercritical extracts from Curcuma longa L. is useful for cosmetic ingredients.

화피(樺皮)의 항산화 효과 및 tyrosinase 저해활성

안봉전,조영제,손준호,박정미,이진영,박태순 한국응용생명화학회 2006 Journal of Applied Biological Chemistry (J. Appl. Vol.49 No.2

생약을 이용한 기능성 화장품

안봉전,김주덕 東國專門大學 1996 金龜論叢 Vol.3 No.1

생약은 우리 조상들이 오래 전부터 사용된 것으로 이것을 화장품에 이용하여 새로운 차원의 천연 한방 화장품 개발이 이루어지고 있다. 그중 미백 효과를 입증하는 타이로시나아제 저해제로서 오매, 계피, 만형자, 하고초등이 미백제로서 그 기능이 우수하였고 보습효과는 당귀, 백지, 국약등이 효과가 좋았다. 피지분비 억제작용으로는 중약, 인삼, 진피등이, 육모와 양모작용으로서는 여정자, 백급, 백지, 하수조등이 효과가 있는 것으로 확인되었다.

감잎(Diospyros kaki folium) 으로부터 분리한 Polyphenol 화합물의 카드뮴 제거효과

안봉전,최청,배만종,손준호,최희진,박무희,우희섭,배두경,조국영 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3

In order to investigate the removal effect of cadmium by polyphenol compound extracted from persimmon leaves(Diospyros kaki folium), animal test was done. Two fractions such as F-1 and F-2 from persimmon leaves were compared with their safety and function. The food intake of group F-1 and F-2 considerably decreased within 1% level. Cadmium addition influenced to rat growth(a tested animal), but food efficiency ratio(FER) wasn't shown any considerable difference in F-1 and F-2, as compared to the control. Cadmium content of liver, kidney and femur considerably decreased within 1% level in F-1 and F-2, compared to the control, cadmium content of liver decreased 25% in F-1, 28% in F-2 also decreased 22% and 25% in kidney. In femur, also decreased 53% in F-1 and 59% in F-2 respectively. The test of cadmium content in feces indicate that the content considerably increased within 1% level in both group F-1 and F-2, as compared to the control(42% in F-1, 54% in F-2). As shown the above results, have seen the removal effect of cadmium by polyphenol compound.

Sanquisorbae officinalis L. 뿌리(地楡)로부터 충치예방 및 미백물질의 분리

안봉전 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

우리는 충치예방 및 미백제 개발 연구의 일환으로 한국산 地楡로부터 새로운 procyanidin류를 분리하였고 thyolysis, desulfurization, methylation, tannase 처리 및 기기 분석에 의해 구조를 결정한 결과 flavan-3-ol 이량체에 gallate 한분자가 결합 된 procyanidin B-3-3-O-gallat 였고 분자량은 Negative FAB-MS; m/z(%): 792[M-H]였다. 이 화합물의 효소저해 효과를 관찰 한 결과 glucosyltransferase는 0.75 mM에서 거의 완벽한 저해를 보여 주었고 tyrosinases는 100μM에서 약 50%의 저해 효과가 있었다. In the course of studies for anti-plaque and whitening agents, novel procyanidin structure isolated from the roots of Sanquisorbae officinalis L.was established by thiolysis, desulfurization, tannase reaction, methylation and spectroscopic analysis. The chemical structure was identified for procyanidin B-3 3-O-gallate containing the dimeric flavan-3-ols, gallate and molecular weight was 792[M-H] by FAB-MS negative ion method. The inhibitory effect on the glucosyltransferase and tyrosinase activity was investigated, novel compound showed complete inhibition at 0.75mM on the glucosyltransferase and tyrosinase was inhibited about 50% at 100 μM.

한국산 Caesalpinia sepiaria 열매(雲實)로부터 Angiotensin Converting Enzyme 저해제 분리 및 화학구조

안봉전 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

고혈압 예방을 위해 우리는 실거리나무 열매(雲實)로부터 ACE 저해제로서 새로운 화합물을 분리하고 기기분석에 의해 구조를 결정한 결과 prodelphinidin B-3 3-O-gallate로서 (+)-gall-ocatechin-3-O-gallate와 (+)-gallocatechin로 결합된 화합물로 구조 결정을 하였다. Prodelphinidin B-3 3-O-gallate의 ACE 저해효과에서 B환에 galloyl이나 gallate 결합한 prodelphinidin류가 저해활성이 증가하는 것으로 확인되었다. prodelphinidin B-3 3-O-gallate는 75μmole에서 약 75.5% 저해 효과가 관찰 된바 이것은 산업화의 가능성을 확인 시켜주었다. For the prevention of hypertensive, a kinds of novel condensed tannin were isolated from the pods of Caesalpinia sepiaria and characterterrized prodelphinidin B-3 3-O-gallate by mechanical analysis. Effect of inhibition on the Angiotensin Converting Enzyme was shown that B-ring, gallyol or gallate compound in prodelphinidins, grown the effect of inhibition. Prodelphinidin B-3 3-O-gallate inhibited about 75.5% at 75μmole, is shown a possibility that is used to industry.

과일과피로부터 폴리페놀 분리에 따른 생리기능연구

안봉전,이진태,곽재훈,박정미,이진영,박태순,손준호,최청 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

Biological activities and anticarcinogenicity of Korean Pear peel were investigated. Electron donating activity and superoxide dismutase(SOD)-like activity of fraction Ⅱ, Ⅲ were up to 90% and 50-60% at 50ppm, respectively. Inhibitory effects on xanthine oxidase were about 80% at 50ppm, breast adenocarcinoma was about 60% at 2,000 ppm, higher Ⅲ than Ⅱ. Inhibitory effect on prostate adenocarcinoma was about 23% at 500 ppm. In conclusion, Korean Pear peel was expected to use as a functional material.

HPLC 분석에 의한 카카오의 폴리페놀 분석과 효소저해 효과

안봉전,이진태 한국식품과학회 1999 한국식품과학회 학술대회 Vol.1999 No.1

한약재를 가미한 녹용추출물의 생리활성에 관한 연구

안봉전,이진태,김상찬,이임식,정종헌,An, Bong-Jeun,Lee, Jin-Tae,Kim, Sang-Chan,Lee, Im-Sik,Chung, Jong-Hun 대한한의학방제학회 2001 大韓韓醫學方劑學會誌 Vol.9 No.1

This study was designed to investigate biological activity of antler extract added medical plants. The scavenging activity of DPPH radical was low scavenging activity at 0.01% concentration. But in the 0.05% and higher concentration, electron donating ability(EDA) is above 50% except Kongindangagam(48.5%) and significantly good above 70% in the 4 extracts. Superoxide dismutase(SOD)-like activity was 44.3% and 45.1% extracts of Ohjayenjongwhangami and M(market sample), Inhibition of xanthine oxidase were above 50% at 0.5% concentration except Boshingiwhangwhangami and from 62.4% to 84.9% in the 4 extracts. Inhibition rate of boshingiwhangwhangami was hasty increased from 33.5% to 77.5% at 1.0% concentration and others the higher concentration, the more increasing inhibition. Angiotension I-converting enzyme(ACE) inhibitory activities were high activity all of extracts. In the 0.5% concentration, ACE inhibition was above 80%. Especially 0.01% concentration of M was presented 81.8%. The study which conducted to investigate the effect of feeding antler extract group for 50 days on sperm concentration, Ca contents of serum, kidney and femur in rats was higher than that saline group.

한국산 감잎로부터 Polyphenol계 생리활성물질 분리

안봉전,배만종,최희진,장운빈,성태수,최청,An, Bong-Jeun,Bae, Man-Jong,Choi, Hee-Jin,Zhang, Yun-Bin,Sung, Tae-Soo,Choi, Cheong 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.4

옛부터 민간 약제로 사용했던 감나무 잎을 식품, 화장품 기능 생물 신소재로 활용하기 위해 감나무잎의 주성분으로 알려진 폴리페놀 화합물을 분리하고 생리활성 기능을 조사하였다. 감나무 잎을 60%의 아세톤으로 추출한 용액을 동결 건조시켜 농도별로 조제하고 glucosyltransferase, tyrosinase 효소저해를 관찰한 결과 glucoslytransferase는 $1.8{\times}10^{-1}$ mg/ml에서 82.84% 저해를, tyrosinase는 0.8 mg/ml에서 21,65% 저해 활성이 확인되었다. Sephadex G-50으로 gel filtration한 결과 fraction 1, 2, 3, 4, 5로 분획되었고 이중 fraction 1, 2에서 효소 저해 활성을 나타내었다. 활성 분획물의 물질군을 확인하기 위해 proteinase K 처리와 멸균처리한 결과 효소저해에 영향은 없어 활성물질군은 단백질군이 아니며, UV spectrum에서 자외선 영역에 최대 흡광치를 보여주므로 페놀링을 갖는 화합물로 추정하게 되었다. 추정화합물을 Sephadex LH-20, MCI-gel 및 Bondapak $C_{18}$ column에서 분리한 결과 F1 fraction에서 compound $1{\sim}4$가지 4종류의 화합물, F2 fraction에서는 compounds 5, 6, F3 fraction에서 compound 7, 8, 9를 순수 정제하여 재결정하였다. 분리된 화합물은 TLC 발색반응에서 flavan-3-ol 골격을 기본으로 하는 축합탄닌 반응을 보였고 전개정도에서 monomer가 2종류, dimer가 3종류, trimer가 1종류로 확인되었다. We purified polyphenols from persimmon leaf and tested their biological activity. The 60% acetone extract was lyophilized and applied to test enzyme inhibition of glucosyltransferase and tyrosinase. GTase was 82.4% inhibited at $1.8{\times}10^{-1}$ mg/ml and tyrosinase 21.7% inhibited at 0.8 mg/ml. The acetone extract was fractionated into F-1, 2, 3, 4, 5 by Sephadex Q-50 gel filtration and the fraction-1 and 2 showed higher enzyme inhibition activity than the other fractions. To the Proteinase K treatment and autoclaving of the two fractions had no effect on the enzyme activity, but these results suggested that active fraction was not protein but phenol ring completed compounds. By Sephadex LH-20, MCI-gel and Bondapak $C_{18}$ column chromatographies, compouds 1, 2, 3 and 4 from F-1 fraction, compounds 5 and 6 from F-2 fraction and compounds 7 , 8 from F-3 fraction were purified and re-crystallized. The purified compounds was assumed to be condensed tannins of frame flavan-3-ol frame on the basis of color reagent reaction and to be a mixture of monomer, dimer and trimer according to TLC analysis.