Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

東皐 李浚慶의 國政運營論 硏究

김준태(Kim, Jun-Tae) 한국동양철학회 2015 동양철학 Vol.0 No.43

This study aims to look into the theory of governance of Lee Jun-kyung who had been Yeongeuijeong (Premier of the Joseon Dynasty) through King Myeongjong and King Seonjo regimes. Lee Jun-kyung is a character with intellectual and political capabilities, who solved several challenges like overcoming the crisis of a political vacuum, evaluated as a leader who opened the so-called ‘Age of Politics by Sarim (Confucian Scholars).’ Based on his own recognition of the major philosophical concepts of Confucianism such as ‘Tian Ren He Yi (Heaven-man Unity)’ or ‘Zhonghe (The Golden Mean),’ Lee Jun-kyung postulated that the king should take the responsibility for the realization. He thought that the political chaos and crisis of the times, like the massacre of scholars was caused by the king’s lack of the cultivation of his own moral character. In other words, the king did not set his mind straight, and made wrong decisions. Thus, according to his judgment, in order to prevent such a calamity from recurring and allow stable governance, most of all, the king should cultivate his own moral character. Lee Jun-kyung emphasized ‘the acceptance of admonition’ because of that. Along with this, Lee Jun-kyung thought that Confucian scholars’ discussion about radical and excessive reform might throw the administration of state affairs into confusion and furthermore, cause a counteroffensive of impure forces. This is the reason why he took a position prioritizing stabilization on major political issues. In other words, political confrontation and conflict should be settled by proceeding step by step gradually, and variables that could cause risks should be prevented in advance. Thus, his argument means that it could bring complete success to affairs, even if it takes time. Lee Jun-kyung’s theory of governance like this marks ‘stability’ with a side dot. The king should cultivate his own moral character for the ‘stability’ of governance and should manage policies and reforms gradually also for ‘stability.’ This line may delay reforms and may cause missing the best time; however, it can exhibit a power to make the reforms have timeliness and minimize side effects on reality. 본고는 명종과 선조 대에 걸쳐 영의정을 지낸 동고 이준경의 국정운영론을 살펴보는 데 목적이 있다. 이준경은 학문과 정치적 역량을 겸비한 인물로, 권력 공백의 위기를 극복하는 등 여러 가지 난제들을 해결하고 소위 ‘사림정치의 시대’를 연 주역으로 평가받는다. 이준경은 ‘天人關係’나 ‘中和’와 같은 儒學의 주요한 철학적 개념들에 대한 나름의 인식을 바탕으로, 그 실현의 책임을 임금에게 상정했다. 그는 士禍 등 당시의 정치적 혼란과 위기의 책임이 임금의 수신이 부족한 데서 기인한다고 생각했다. 임금이 자신의 마음을 바로잡지 못했기 때문에 잘못된 결정을 내렸다는 것이다. 따라서 그러한 참화가 재발하지 않도록 하고 국정이 안정적으로 운영될 수 있도록 하기 위해서는, 무엇보다 임금이 수신에 힘써야 한다는 것이 그의 판단이다. 이준경이 納言을 강조한 것도 그래서이다. 아울러 이준경은 사림의 급진적이고 과격한 개혁 논의가 국정을 혼란에 빠트리고, 나아가 小人들의 역공을 유발할 수 있다고 생각했다. 문소전 배향, 삭훈, 붕당 등 주요 정치적 이슈들에 있어서 그가 안정 우선의 입장을 취한 이유이다. 점차적으로 단계를 밟아감으로써 정치적 대립과 알력을 해소하고, 위험을 일으킬 수 있는 변수들을 사전에 차단해야 한다는 것이다. 그래야 비록 시간이 걸리더라도 일을 온전히 성공시킬 수 있다는 것이 그의 주장이다. 이러한 이준경의 국정운영론은 安定에 방점이 찍혀있다. 군주가 수신을 해야 하는 이유도 국정의 ‘안정’을 위해서고, 정책이나 개혁을 점진적으로 운용해야 하는 것도 ‘안정’을 위해서인 것이다. 이러한 노선은 개혁을 지연시키고 자칫 最適期를 놓치게 할 우려도 있지만, 개혁이 시의성을 갖추고 현실에서의 부작용을 최소화하도록 하는 힘을 발휘할 수 있다.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

Nematicidal and egg‐hatching inhibition activities of Kaempferia galanga rhizome‐derived materials and constituents toward root‐knot nematode (Meloidogyne incognita)

Tae‐Kyun Hong,Kym‐Na‐Ra Hwang,Jun‐Ran Kim,Young‐Joon Ahn 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

The toxicity of Kaempferia galanga rhizome materials and constituents against Meloidogyne incognita second‐stage juveniles (J2) and eggs were examined. The active principles of K. galanga rhizome were identified as the phenylpropanoids ethyl (E)‐cinnamate (EC, 1) and ethyl (E)‐p‐methoxycinnamate (EMC, 2) by spectroscopic analysis. Results were compared with those of carbofuran, fosthiazate, and metam‐sodium. In direct‐contact mortality bioassay, EC (LC50, 0.037 mg/ml) was the most toxic constituent, followed by EMC (0.041 mg/ml). EC was more effective than carbofuran (LC50, 0.092 mg/ml) but less active than fosthiazate (0.002 mg/ml). EC, egg hatch was inhibited 100, 93, and 87% at 125, 62.5, and 31.25 μg/ml, respectively. EMC caused 100, 81, and 75% inhibition of egg hatch at 125, 62.5, and 31.25 μg/ml, respectively. The inhibition of two phenylpropanoids were similar or more inhibition to that of either carbofuran or metam‐sodium but was lower than that of fosthiazate. In contact + fumigant mortality bioassay, EC and EMC treatments resulted in 86 and c 73% mortality at 0.5 and 0.125 mg/g soil, respectively. The lethality of these phenylpropanoids was almost similar to that of either carbofuran or metam‐sodium but was lower than that of fosthiazate. In vapor‐phase mortality bioassay, EC and EMC were more effective in closed container than open containers, indicating that the mode of delivery of these compounds was, in part, a result of vapor action. K. galanga rhizome‐derived materials, merit further study as potential nematicides and hatching inhibitors for the control of M. incognita populations.

간문부 담관암 환자에서 경피경관 양측성 금속 배액관의 효과

김금래,김주형,박원규,장재천,조재호,김태년,김준화,장병익 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Background: The purpose of this study was to determine the effectiveness of percutaneously place self-expanding metallic stent for the relief of biliary obstruction in patients with hilar cholangiocarcinoma. Materials and Methods: From November 2001 to December 2004, 48 patients with hilar cholangiocarcinoma were prospectively studied. After percutaneous placement of bilateral self-expanding, uncovered metallic stents, follow-up evaluation was carried out until July 2005. Results: There were 4 cased of Bismuth type Ⅱ,21 cases of Bismuth typeⅢa 8 cases of Bismuth type Ⅲb and 15 cases of Bismuth type Ⅳ. Stent placement was technically successful in all patients All patients had satisfactory biliary drainage, resulting in one week drainage rate of 72.8% and final drainage of 91.9% There were 12 cases (21.3%) of abdominal pain requiring analgesics and 1 case (7.1%) of cholangitis; both were successfully managed with conservative treatments. Late complications occurred in four patient (8.3%), including two patients with cholangitis, one patient with liver abscess, and one patient with biloma; all were appropriately managed by percutaneous drainage. The average length and median durations of stent patency and median survival time were 303 days (range, 60~815) and 338 days (range, 60 ~1175), respectively. Conclusion: Placement of a percutaneous metallic stent is an effective and safe method of palliation of patients with hilar cholangiocarcinoma.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

하악골에 발생한 골막 골육종

김태우,김승범,권혁찬,문선재,윤정훈,김형준,차인호,육종인,김진 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Periosteal osteosarcoma is a distinct entity of malignant bone tumor with characteristic clinical, morphological, and histological features within the group of juxtacortical osteosarcoma. Periosteal osteosarcoma is predominantly located in the tubular long bones, and extremely rarely involving the jaws. A case of periosteal osteosarcoma of the right mandible is presented. A 27-year-old woman complained of the gingival swelling and bleeding tendency of the right posterior mandible. Clinical examination revealed a reddish brown strawberry-like swelling on the affected mucosa, which measured 1.5㎝×1.5㎝. The tumor was located on the lingual cortex of the mandible and extended into the surrounding soft tissues. Microscopically, the tumor consisted exclusively of atypical chondroblastic cells with a small osteoblastic area. A minimal bone marrow involvement was noted and the adjacent cortex was free of tumor. These findings suggested that the tumor originated from the periosteal cambium layer, which lies between the periosteal fibrous layer and the cortex of mandible.

Identification of a novel peptide that selectively binds to Kidney Injury Molecule-1 (KIM-1) by phage displayed peptide library

Tae-Jun Kwon,Joon-Suk Park,Sang-Hyun Kim,Enamul Haque,Byung-Heon Lee,Sang Eun Kim,Choong-Yong Kim,Sang Kyoon Kim 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

WMI 기술을 이용한 컴퓨터 원격제어 시스템

김정규;김형준;전용화;최병문;서재현;김태공 인제대학교 2012 仁濟論叢 Vol.27 No.1

In organization and company managing a lot of computers, it is difficult to manage the computer system, because it is not easy to recognize exceptions of distributed workplace such as departments and laboratories. Existing computer management systems control many computers through manager using server computer for management in field. Therefore they have the characteristic of strong locality. In this paper, we develop computer remote control system which supports convenient features and non-locality to managers by broadening effective ranges of computer management. We use Web-Site and Android Platform to develop the environment of client management regardless of multiple clients and locality. Moreover, we use WMI (Windows Management Instrumentation) to recognize and control the status of client PC without a impact on computer users.