Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

太谿穴의 臨床 活用에 대한 文獻考察

구성태,송문영,강정묵,김용명,박보라,이은석,홍정아,김경식,손인철 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Literally, the mean of Tae-gye is highest brook. And on the basis of the acupuncture theory, Tae-gye point is the Soo earth point and source point of the Kidney Channel as well. In addition, Tae-gye point is one of the Yang-Returning Nine points. We were trying to study bibliographically on the Tae-gye point because Tae-gye point can be used very broadly across the symptoms in the theoretical aspect described above. As a results, we found out that according to the classical books of acupuncture, Tae-gye point is entering point of the Meridian Water as a source point and can be used at both Kidney-Sufficient Syndrom and Kidney-Deficient Syndrom. And Tae-gye can be applied to the disease of kidney or bladder that is urogenital symtoms, Also, Tae-gye is an useful option of tooth-ache, asthma, indigestion, constipation, edema etc whose cause is related with decrease of Kidney ki.

Effects of Variation of Heat Flux Released from the Meniscus on the Surface Shape of the Solidified Shell During Continuous Casting

Yong‑tae Kim,Kyung‑woo Yi 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Mold oscillations, widely used in continuous casting for infiltrating the mold flux between the mold and the solidified shell,change periodically the heat flux from the meniscus to mold. These variations of the heat flux affect the solidification behaviornear an initial solidification position. Because the surface shape of the solidified shell is influenced by the initial solidificationbehavior, it is important to understand the relation between the mold oscillation, the heat flux and initial solidificationbehavior. In the present study, we developed a numerical model that simulates the flow and thermal behavior of the moldflux near the meniscus. Using this model, we analyze the effects of the variations of the heat flux from the meniscus on theinitial solidification position and suggest the formation mechanism of the oscillation marks (OMs) and the hooks. Also, wewill present quantitative results about the depth of the OMs and the length of the hooks at the conditions used in the presentstudy. The factors that influence the hooks’ types and the causes of the irregularity of the shell surface are also discussed.

Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

학업/비학업자아개념, 학교태도 및 학교적응간의 관계 탐색

金龍來,金兌垠 弘益大學校 敎育硏究所 2001 敎育硏究論叢 Vol.- No.18

The purpose of this examination was to find out the relationship among secondary school learners' academic/nonacademic self-concept, school-related attitudes and school-related coping. And in order to achieve the purpose of this examination sampled middle/high school learners in Seoul and other areas. The instruments used for the data collection were 「Self-Concept Scale」 (Kim, Yong-Rae, 1995), 「School-Related Attitudes Scale」 (Kim, Yong-Rea, Lee, Hye-Won, 1996) and 「School-Related Coping Scale」 (Kim, Yong-Rae, 2000). The collected data analyses were performed using SPSS Win (10 V) program, and analysed Mean score (M), Standard Deviation (SD), Zero-Order Correlation (γ) and Multi-Regression Analysis. All statistic values were tested by t-test and F-test in the significant level of p< .05. And as for the major findings of this examination, only three variables - 'school general self-concept', 'teacher-related attitudes' and 'instruction related attitudes' among all research variables for this examination were significantly positive influences on Korea secondary school learners' "school-related coping" respectively.

자아개념과 정서지능의 관계

김용래,김태은 홍익대학교 2002 敎育硏究論叢 Vol.- No.19

The purpose of this study was to conceptualize the `Emotional Intelligence' and the 'Self-Concept' through an analysis of the related literature. and to find out the relationships between secondary school learners' emotional intelligence(EI) and self-concept(SC). And in order to achieve the purpose of this study analysed the related literatures and the subjects were sampled voluntarily 320 middle and high school learners in Seoul and other areas. The scales/instruments used for data collection were 「Emotional Intelligence Scale」 (Moon, Yong-Lin, 1996) and 「Self-Concept Scale] (Kim, Yong-Rae, 1995), the collected data processing was performed by SPSS/PC+ program, and analysed zero-order correlation between two variables and multiple regression, and all statistic value (r, R^2, R^2ch, andβ) were tested by F-test and t-test at the significant level of p < .05. As for the major contents of the findings in this study were as follows: First, "Self-concept is the level or degree of the evaluative attitudes and beliefs about 'the self' based feelings and views that was formed through interaction between self and significant others and various experiences in environment/situation" Second, "Emotional intelligence is multifactorial array of interrelated emotional, personal, and social abilities - the ability to accurately perceive and appraise self, to be aware of and understand self-emotions, to effectively manage self-emotions, to control self-emotions, to adapt and adjust self-feelings and thoughts to new situations, to solve self-personal and interpersonal problems, to be aware of and understand others emotions, and to relate well others". And addition to these abilities, emotional intelligence may be include following the abilities - the ability to be positive and to look at the brighter side of life, to feel content with self, others, and life in general, to be self-reliant and free of emotional dependency on others, to identify with and feel part of our social groups, and to achieve goals and actualize self-potential. Third, the correlation between learners 'emotional intelligence' and 'self-concept' was relatively in meaningful positive relationships. Learners nonacademic self-concept was more influence on emotional intelligence than academic self-concept. Personality self-concept and family self-concept among nonacademic self-concept subfactors influenced on emotional intelligence and its subfactors respectively. And the personality self-concept was in meaningful higher power of influence on the emotional intelligence than the family self-concept.

당뇨병성 합병증을 가진 환자에서 혈증 Erythropoietin 농도

김동규,유기동,허광식,김상용,윤성호,조영신,권용은,김태원,김건영,정종훈,배학연 조선의대 1998 The Medical Journal of Chosun University Vol.23 No.1

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.