정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

消癌去痰益氣湯의 cyclophosphamide 부작용 형성 억제효과

홍종희,류기원,류봉하,윤상협,김진성 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-

Object : It is well known that modern chemotherapy against cancer has side effcts to a living body, especially hemopoietic and immunologial disfunctions. However, there are no effctive ways to reduce them.Recently, traditonal Korean herb medicine has been repoted to have some biological modifying responses. Therefore, we hypothesized that additional application of herb medicine during chemotherapy is more effective to reduce its side effects. While we were studying the effects, we have observed the inhibitory effect of Soamgudamikgitang on formation of side effects derived from Cyclophosphamide, it has been used in clinical practice at Kyung Hee Medicd Center.Methods : We injected 200㎎/㎏ of Cyclophosphamide, one time, to an experimental group, consisting of ten mice. We divided them into eight groups: normal, CPX, SAKT 2㎎, SAKT 10㎎, SAKT 50㎎, SAKI 2㎎+CPX, SAKT 10㎎+CPX, SAKT 50㎎+CPX. We injeted Soamgudamikgitang seven days, five days, three days, and one day before we injected CPX. One day, three days, and five days after CPX injection, we injected Soamgudamikgitang again and then killed all the mice.The parameters determined in this experiment were daily body weight liver and spleen weight, RBC, WBC, and platelet for hemopoietic dysfunction and AST, ALT for hepatotoxicity, BUN, creatine for renal toxcity, Iymphocyte proliferation activity and lymphocyte subsets for immunological toxvity.Results : We have found that Soamgdamikgitang has inhibitory effects on the formation of Cyclophosphamide 's side effects. Significant differences between the group, which contained Cyclophosphamide, and the other group, which contains Cyclophosphamide and 2, 10, 50㎎ of Soamgudamikgitang respectively were observed. Platelets(2㎎ of Soamgudamikgitang, p<O.05 ;10㎎, p<0.Ol ;50㎎, p<0.001), liver weight(SOw p.001), spieen weight(10mg, p<0.05), AST(all groups, p<0.01), ALT(2㎎, p<001 ;10㎎, p<O.05 ;50㎎, p<0.01), BUN(2㎎, p<0.01 ;50㎎, p<0.05). Althought immunological in both lymphocyte proliferation and its subsets were not observed, which shows that Soamgudamikgilang has a strong effect on T cell activities Conclusions : From the above results, we can expect that the combined therapy of Soamgudamikgitang and Cyclophosphamide is more effective for treating cancer Patients.

황정(黃精) 추출물의 화학구조결정에 관한 연구 (Ⅰ)

윤중호,박주희,김정주,권기락,안철진,주우홍,강진호,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1

본 연구에서는 황정 속에 포함되어 있는 생리활성 물질을 hexane, CHCl₃과 n-butanol층에서 각각의 성분들을 추출하였고, hexane층에서 분리된 화합물 I과 H 중에서, 화합물 I의 봐학구조를 ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC 스펙트럼 및 MS 스펙트럼 등의 분광학적인 방법에 의해 결정하였다. 화합물 I의 구조는 9,12-(9E, 12E)-octadecadienoic acid 임을 확인하였다. In this Paper, biologically active compounds were extracted using organic solvents as hexane. CHC1₃, n-butanol to give each component. Chemical structure of compound I was characterized using ¹H-nmr, ??, DEPT135, COSY, HMQC, HMBC spectrum and MS-spectrum, in separated compound I and Ⅱ from hexane layer. Finally, chemical structure of compound I was determined as 9,12-(9E,12E)-octadecadienoic acid.

室內디자인의 新構成主義的 傾向에 關한 硏究

尹道根,金洪基 弘益大學校 1990 弘大論叢 Vol.22 No.2

Recently, the interior design, closely related to architectural trends, has been identified its speciality as a prefession, and increasingly extended into scopes. Therefore, those circumstances make us redefine it's essential points and theories of interior design. It accordance with those contexts, for redefining principles of spatial composition in contemporary interior design, and proposing the direction of it's development, this paper examined how the designs of neo-constructivism, the most prevailing since post-modernism in terms of formation of architectural spaces, apply to the interior space. Probably, after these examinations, we would make through a part of new techniques of spatial configurations and try new directions of the scope in interior design and architectural design. Therefore, the main points of the examinations in this paper, based on the essence of interior design and the trend of contemporary architectural design, underlined in the scopes of development, design elements, compositional techniques, and the case study and analysis of neo-constructivism.

유전자재조합 대장균으로부터 Bacillus sp. 79-23의 Cellulase 정제와 특성분석

윤기홍 우송대학교 부설 산업연구원 1999 산업연구 Vol.1 No.1

Carboxymethyl celluase (CMCase) was purified from cell-free extract of the recombinant Escherichia coli carrying a Bacillus sp. 79-23 CMCase gene (celS) by DEAE-Sepharose and phenyl-Sepharose column chromatography with specific activity of 400 U/mg protein. The molecular mass of the purified enzyme was estimated to be approximately 37.5 daltons by sodium dodecyl sulfatepolycarylamide gel electrophoresis. The viscosity of carboxymethyl celluose was dramatically decreased by the purified enzyme identifying that the purified enzyme is a CMCase, while the enzyme was known to be active on para-nitrophenyl-β-cellobioside. The CMCase activity was markedly inhibited by cellobiose, but was not inhibited by glucose. The enzyme activity was activated by divalent metal ions such as Mn²+ and Cu²+, but was completely inhibited by Hg²+. The enzyme was very stable below 50℃ and retained 80% of its maximum activity after incubation for 3 hours at 55℃, suggesting that few problems are in handing the Bacillus sp. CMCase as industrial enzyme at ambient temperature.

대학생들의 안전교육 및 안전의식에 관한 연구

홍기헌 ; 윤석호 김천대학교 2014 김천대학교 논문집 Vol.35 No.-

The purpose of this study is to figure out current status of safety education until today and safety consciousness of university students. For this study, the 300 university students were taken among freshmen and sophomores in gimcheon university. Furthermore it is try to realize the necessity of additional safety education in universi-ty and the unification of emergency call numbers. The results are as follows: 1. It was showed that the most students prefer unified emergency call number not divided. 2. Marine accident safety education was lowered compared with other life safety ducations such as fire, earthquake, and traffic safety educations. 3. It was showed that many university students wish additional actual safety educa- tion in university as academic subjects. 4. University students consider that Sewol passenger ship accident was caused by eficient of vocation and safety frigidity of crew and company staffs. And in order to revent this kind of accident, crew vocation should be built up, students safety duca-tion should be strengthened and government agency should watch severely the hip remodelling

Mannanase를 생산하는 Bacillus속 균의 RAPD-PCR 분석

윤기홍 우송대학교 2004 우송대학교 논문집 Vol.9 No.-

가정에서 제조된 된장에서 mannanase 생산성이 높은 균으로 분리된 9종의 균주는 모두 포자를 형성하며 그람양성 간균으로 Bacillus속에 속하는 것으로 추정된다. 이들 분리균간에 유전체의 유사성을 조사하기 위해 10 nucleotides로 구성된 oligonucleotides 6 종과 12 nucleotides로 구성된 oligonucleotides 2종을 primer로 사용하여 Random Amplified Polymorphic DNA-중합연쇄반응 (RAPD-PCR)의 조건을 검토한 결과 40℃보다는 30℃와 35℃에서 annealing 반응을 수행하는 것이 효율적이었다. RAPD-PCR에 의해 증폭된 DNA를 agarsoe gel 전기영동으로 분석한 결과 primer에 따라 증폭된 DNA의 양과 그 크기의 다양성이 달랐으며, 분리균에 따라서도 특이한 증폭 DNA가 관찰되었다. 균주에 따라 증폭된 DNA를 비교한 결과 된장에서 분리된 9 종의 균주는 Bacillus licheniformis와는 달랐으며, Bacillus subtilis와 유사하였다. 특히 분리균 중 5 종류는 B. subtilis와 유사도가 매우 높았으며, 분리균간에도 서로 유사도가 높은 균이 있었지만 동일하지는 않은 것으로 확인되었다. 따라서 RAPD-PCR 방법에 의해 균주간 유사성을 비교하는 것은 다양한 미생물 자원을 확보하는데 유용한 방법이라고 여겨진다. Nine bacterial strains producing the extracellular mannanase were isolated from Korean soybean paste. The isolates are regarded as Bacillus on the basis of their morphological properties, which are gram positive and rod in shape with spore. To examine the similarity between genomes of the isolates, Random Amplified Polymorphic DNA - Polymerase Chain Reaction (RAPD-PCR) was performed with 6 kinds of oligonucleotides consisting of 10 nucleotides and 2 kinds of oligonucleotides consisting of 12 nucleotides. As a results of RAPD-PCR at different annealing temperatures such as 30℃, 35℃ and 40℃, many different DNA fragments were generated at 30℃ and 35℃ more than 40℃. Relatedness of the nine isolates from soybean was determined by comparing the amplified DNA fragments between the isolate strains, Bacillus subtilis and B. licheniformis, expecting that the nine isolates are different from B. licheniformis and five of them are very similar to B. subtilis. It was also found that all isolates are different strains. Therefore, RAPD-PCR is regarded as a useful method for preparing the bank of the diverse microorganisms from nature.

당뇨병환자에서 게이트심장혈액풀신티그라피를 이용한 심기능 평가

윤상임,송치운,이진홍,안미애,성기양,송민호,이강욱,신영태,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

Major cardiovascular complications of diabetes are coronary atherosclerosis, diabetic dilated cardiomyopathy, autonomic neuropathy and those are major causes of morbidity and mortality in diabetic patients. Gated blood pool heart scan is noninvasive and useful method for evaluation of functional status of heart in diabetics. We evaluated 52 patients with diabetes and divided 3 groups. Group 1 were 11 patients without proteinuria or with proteinuria less than 550mng during 24 hours. Group 2 were 9 patients with proteinuria more than 550mg during 24 hours and group 3 were 32 patients with endstage renal diasese due to diabetes. We performed 99mTc-HSA cardiac gated blood pool scan and used left ventricular ejection fraction(LVEF), peak ejection rate(PEF) to indices of LV systolic function and peak filling rate(PER) to index of LV diastolic function. The results were follows : 1) LVEF, PER were significantly lower in diabetics with ESRD than diabetics without ESRD, but there were no significant difference between normal controls and diabetics without ESRD 2) PFR was significantly lower in diabetics than normal controls, but there were no significant differences in diabetics with or without nephropathy. 3) There were negative correlation between PER, PFR and duration of diabetes. On the basis of results, PFR is a LV functional index of GBPS which can disclose early change of LV dysfunction in patients with diabetes.

초등 사회과 3학년 지역화 교수·학습자료개발 연구

홍기대,손정선,김언식,김용조,이광성,김경운 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

The purpose of this study is in developing locally-oriented teaching-learning materials to motivate students to effectively take part in activities using map and in improving the field work of social studies to plan a map learning and to give them the ability of practice. It represents the right order involved in teaching the activities using map after doing preceding learning : classroom, playground, and the environs of the school including to some learning methods such as a puzzle map, a correlative of learning. Field trip study planning and lesson-plan are designed in case of Sewage disposal plant, Duknam filtration plant, Bonchon industrial complex relating to field trip study. There are some effects expected which follow through this study. First, teachers are able to draw learner's interest in finding learning sources directly through the community where they live. Second, learners have active participation in class, motivation, drawing their interests through map learning by doing preceding study of learner-centered. Third, teachers can make use of it as useful tools to get high achievement goal with self-direct learning.

술중 전향적 대장세척술과 대장아전절제술을 이용한 폐쇄성 좌측 대장 및 직장암의 일차절제 및 문합술

윤완희,홍기훈,송인상 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The proper surgical management of obstructing left colorectal cancers in an issue of debate. Staged operation including proximal colostomy has generally been accepted as a safe and standard method of management. But staged colonic procedures are associated with significant morbidity and mortality, and many patients, unfit for further surgery, are left with a permanent colostomy. Many other methods such as the use of long intestinal tube, transanastomotic obturation balloon colostomy, intracolonic bypass tube (Coloshield) were developed, but they have failed to gain wide accepance. We have already reported the initial results of intraoperative antegrade colonic irrigation for one-stage operation in obstructing left colorectal cancers. In this investigation, we evaluated the results of prospective trials of primary resection and anastomosis using intraoperative colon irrigation and subtotal colectomy in obstructing loft colon and rectal carcinomas. During recent five years, 19 cases of obstructing left colon and rectal carcinomas which could not received regular colon preparation due to marked abdominal distension were enrolled to this study. There were 12 men and 7 women, and mean age was 61. 14 of the 19 patients which tumors located below mid-descending colon were subjected to primary resection and anastomosis using intraoperative antegrade colonic irrigation, 4 patients which tumors located above the mid-descending colon were treated with subtotal colectomy, and remaining one patient which tumor located upper rectum was performed subtotal coloectomy because of underlying colonic ischemia. There was no mortality and significant postoperative complications such as anastomotic leakage, pelvic abscess of intraabdominal sepsis which seemed to related with anastomotic dehiscence. Therefore, primary resection and anastomosis using intraoperative colonic irrigation of subtotal colectomy depending on tumor locations in cases of obstructing left colon and rectal carcinomas might be useful methods to obtain safe one-stage restorative colorectal resections