少陰人 補中益氣湯의 效能에 關한 實驗的 硏究

尹相協,朴東遠,?基遠 慶熙大學校韓醫科大學韓醫學硏究所 1987 慶熙韓醫大論文集 Vol.10 No.-

구취 환자의 특성 및 관련 인자에 대한 연구

윤상협,류봉하,류기원,김진성,Yoon, Sang-Hyub,Ryu, Bong-Ha,Ryu, Ki-Won,Kim, Jin-Sung 대한한방내과학회 2004 대한한방내과학회지 Vol.25 No.4

Objectives : Halitosis is a common human condition, exact pathophysiological mechanisms of which are unclear. This study, which investigates halitosis patients' characteristics and their related factors, was done with intent to establish a foundation for the Oriental Medical treatment of halitosis. Methods : 329 patients were surveyed by reviewing medical charts and questionnaires from the East-West Halitosis Clinic of Kyunghee Medical Center from May 1, 2001 to December 31, 2002. Sex, age, illness duration, coating on the tongue, postnasal drip, globus pharyngeus, indigestion, condition of feces, halimeter measurements and results of the salivary scans were analyzed. Results : The thick and yellowish coating on the tongue was not a major cause of halitosis in the patients studied. Among the halitosis patients, 21.6% complained of postnasal drip, 15.6% of globus pharyngeus, 37.4% of indigestion, and 23.8% of diarrhea or constipation, suggesting the possibility of extra-oral origin in oral malodor. Conclusions : This study suggests that, when treating halitosis patients, intra-oral causes of halitosis aside, Oriental Medical treatments such as solving qi-stasis(氣鬱), augmenting deficiency of the spleen qi(碑氣虛) and treating gastric fever(胃熱) constitute effective management for oral malodor patients.

류마티스 관절염의 수술적 치료

윤상협,김신윤 대한의사협회 2010 대한의사협회지 Vol.53 No.10

Alarge number of patients who are diagnosed with rheumatoid arthritis undergo a chronic and progressive course. Surgical treatment is often needed for these patients, who have not responded to medical treatment. The aim of surgical intervention in rheumatoid arthritis is to restore function and quality of life by preventing joint destruction, correcting deformity, relieving pain, and making cosmetic improvements. There are many surgical options, including synovectomy,osteotomy, arthrodesis, resection arthroplasty, joint replacement, surgeries for the treatment of the tendon involved (repair, transfer, graft), and cervical spine surgery (fusion or decompression). The selection of the optimal surgical option requires consideration of not only the articular status and the degree of regional deformity involved, but also the physical status and age of the patient. In addition, timely surgical intervention is important because a delay of surgery often results in poorer functional outcomes and an increase in postoperative complications. Early referral to orthopedic treatment can provide better functional outcomes for patients with rheumatoid arthritis. Precise prediction of the disease progress and selection of the optimal treatment option are needed for excellent results to be expected. Therefore, a cooperative and multidisciplinary treatment strategy should be made among the relevant teams, such as physical medicine,rheumatology, orthopedics, physical therapy, occupational therapy, social work, and psychology.

뇌경색유발(腦梗塞誘發) 흰쥐의 국소뇌혈류량(局所腦血流量)과 뇌부종(腦浮腫)에 대한 이진탕(二陳湯)의 수(水) 및 메탄올추출엑스의 효과(效果)

윤상협,민병일,류재환,Yun, Sang-Hyeop,Min, Byeong-Il,Ryu, Jae-Hwan 대한한의학회 1996 대한한의학회지 Vol.17 No.2

For the purpose of developing a new drug against cerebral infarction, we chose Ijintang which is frequently applied in the field of Jung Pung of traditional medicine. From this formula, water extract and methanol extract were prepared respectively. Animals were divided into three groups; control group, water extract medicated group, methanol extract treated group. water extract was given 195mg/kg orally and methanol extract, 165mg/kg for 2 weeks in case of cerebral edema and 1 month in regional cerebral blood flow. On the regional cerebral blood flow, the drug medicated groups showed no significant changes as compared gith control group. There was no significant difference on the cerebral edema among the groups. According to the results above, it is suggested that oral medication of water extract and methanol extract from Ijintang have no effect on the change of rCBF and edema after acutely induced cerebral infarction. However, it is thought that modification of administration method and development of new animal model for traditional medicine are needed.

기능성 소화불량증 환자에 대한 반하사심탕 엑기스제의 임상효과

윤상협,류봉하,류기원,김진성 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Objective : We evaluated the therapeutic effect of Banwhasasimtang dry extract powder on gastrointestinal symptoms and its side effects in patients with functional dyspepsia. Methods : Patients with functional dyspepsia, defined as discomfort in the epigastrium. A total 45 patients participated participated in this study. Dyspeptic symptoms ratio was obtained by patients, total point of symptom score / total 21 points x 100(%). On each of symptom after 4 weeks administration of Banwhasashim-tang was made by variation of symptom score: If symptoms score of posttreatment is lower than that of pretreatment, It is improved ; Otherwise aggravated, and if there is no change between them, unchangeable. When a full health condition of normal control is assumed 100%, relative health condition of dyspeptic patient could be calculated from 'full health condition- dyspeptic symptoms ratio. Therefore a whole satisfaction rate on symptom improvement was presented with variation of a relative health condition to dyspepsia between pretreatment and post. Results : Banwhasashimtang significantly unproved symptoms in patients. The significance was shown in both all seven items(p< 0.01, respectively) and 19.2% (p<0.01) of increase of a whole symptom improvement. There is no remarkable side-effect in all laboratory test, excepting 1 case abdominal pain another one case of recurred pain of chronic prostatitis. Conclusion : Banwhasashimtang extract provides symptomatic relief in functional dyspepsia and is no remarkable side-effect drug.

女性 中風에 있어서 衝脈의 役活에 대한 豫備的 實驗硏究

尹相協 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1998 東西醫學硏究所 論文集 Vol.1998 No.-

The aim of this study was to assess ischemic effect on sex hormonal alteration(17βestradiol, LH, FSH) in ovarectomized rats. Rats were divided into 3 groups; normal group(n=10), ovariectomized group without cerebral ischemia(OV group, n=10), and ovariectomized group with cerebral ischemia (OVCI group, n=10). Sex hormons in both ovariectomized groups were determined on 15th day(n=5) and 30th (n=5) after ovariectomy. For normal data and its comparison, the same numbers of rat in normal group were killed on the same day. Cerbral ischemia w as induced by electrocauterization of rhinal fissure portion of left middle cerebral artery and 1 hour ligation of both common carotid artery. After l hour ligation, right common carotid artery was reperfusioned and left artery was occluded permantly. after finishing ischemia, rats were returned to their cages. It have been found that concentration of 17β-estradiol, LH,and FSH were more lower in the cerebral ischemia treated ovariectomized group than in the non treated ovariectomized (p<0.05) This study shows that cerebral ischemic injury can inhibit the compensatory function of adrenal gland on estrogen production and that also impair the hypothalmus and hypophysis functions in the ovariectomized rats.

차음상자와 전자청진기로 기록된 장음에 의해서 깨어 있는 흰쥐의 위수축력 측정

윤상협,Yoon, Sang-Hyub 대한한방내과학회 2014 대한한방내과학회지 Vol.35 No.4

Objectives: The aim of this study was to investigate whether 1) variation of bowel sounds recorded stably through an electronic stethoscope in a sound insulation box can be related with that of gastric contraction and 2) if they are thus useful tool in the measurement of the gastric contractility in awake rats or not. Methods: Electrical potentials of both electronic stethoscope of bowel sound and force transducer were recorded simultaneously and continuously in the sound insulation box for the starting 30 min of basal state, and then 30 min of 0.2 ml normal saline administration, finally 30 min of 0.2 ml mosapride citrate solution (100 mg/Kg) in rats. Each motility index of normal saline or mosapride citrate treatment was presented with ratio against the basal state by using integrated electrical potentials. Results: A pattern of significance of gastric contractility between bowel sound and force transducer was showed analogously. Conclusions: The amplitude of bowel sounds recorded by the electronic stethoscope related with the intensity of gastric contractions. This confirms that a sound insulation box and electronic stethoscope are useful tools in the measurement of the gastric contractility of awake rats.

腦卒中後 腦萎縮에 대한 調經論的 接近

윤상협,이종수 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-

Objective : The aim of this study was to investigate the neuroprotection effect of estrogen on brain atrophy following cerebral infarction. Method : All animals in this study were classified into 4 groups; ovariectomy group (OVXgroup), cerebral infarction group (INF group), combination ovariectomy and cerebral infarction group ( OVX + INF group), and naturally intact group for control data (NOR group ). Cerebral infarction was made by Chen's method with some modification. Ovariectomy was performed by Wayforth's method. Experimental data for each group was collected at 15days, 1 month, 3 months, and 6 months after starting observation. Serum 17β-estradiol(E2) was determined by radioimmunoassay. Brain volume was measured and calculated with image analysis. Each brain was sliced at intervals of 2mm in chamber after 30 min of freezing in refregerater. Cerebral volume was obtained by sum of volume of each slice level, which was mean area × 2mm. Results : Cerebral ischemia was fou8nd to decrease the serum concentration of 17β-estradiol and to inhibit the physiologically conpensatary function of the ovariectomized rats. Also we found that deprivation of estrogen have resulted in more severe cerebral atrophy followed by cerebral infarction. Conclusion : It is suggested that estrogen has a neuroprotection effect on cerebral atrophy following cerebral infarction.(J Korean Oriental Med 2000;21(4):9-15)

흰쥐의 虛血性 中風證 모델에 관한 硏究 : 중뇌동맥 원 및 근위부폐색에 대한 양측 경동맥 1시간 결찰의 효과 Effect of 1 hour occlusion of CCA on the distal or procimal MCA occlusioned site

윤상협 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1997 東西醫學硏究所 論文集 Vol.1997 No.-

With the purpose of producing easily the basal ganglia infarction into Chen's, scerebral ischemic model which is almost cortical infarct made by the ligation of distal part of MCA and 1 hr obliteration of both common carotid arteries in rat, the MCA obstruction was extended between rhinal fissure and olfactory tract with electrocauterization in place of 10-0 silk suture ligation of distal part of MCA. Both original Chen's model and modified Chen's have shown the cortical infarction in dorsolateral & lateral frontoparietal cortex, but not any infarction in basal ganglia. However, the modified Chen's model have shown the effect of average 12% increase in cortical infarct than that of original Chen's model. This experimental results suggest the modified Chen's model can not reduce the blood flow of the lateral lenticulostriatal artery enough to make the basal ganglia infarction and that blood circulation of basal gagglia under its condition is probably being kept partly through the posterior cerebral artery via vertebral artery. Therefore, The follow-up observation on ischemic time lapse would be needed.

와송의 수종 암세포에 대한 항암작용 연구

윤상협,류봉하,류기원,김진성,Yoon, Sang-Hyub,Ryu, Bong-Ha,Ryu, Ki-Won,Kim, Jin-Sung 대한한방내과학회 2005 대한한방내과학회지 Vol.26 No.2

Background: Cancer reseach is done in earnest world-wide, because cancer is one of most threatening diseases to humans. Orostachyos Herba is a widely used herb that has long been in use in Korea as an anti-inflammatory and anti-cancer therapy. The purpose of this study is to verify any anti-cancer effects on stomach and liver cancer in vitro. Materials & Methods: AGS and KATO III stomach cancer cells and Hep3B and HepG2 liver cancer cells, all obtained from Korean Cell Line Bank, were used. The boiled extract of Orostachyos Herba(20 and 40 microliters) were injected into cultures and observed at 0 hours, and at 24-hour intervals up to 96 hours. The destruction of stomach and liver cancer cells was measured through Trypan blue exclusion testing. The suppression on viability of stomach and liver cancer cells was observed, and anti-cancer mechanisms was examined by analyzing the cell cycle. Results: In morphologic change, AGS, KATO III, HepG2 and Hep3B showed some of the withdrawn and floating appearance that is typical in cellular imparment. AGS, KATO III, HepG2 and Hep3B showed more destruction of stomach cancer cells in each test group than in the control group to a statistically significant degree. Analysis of the cell cycle after introduction of Orostachyos Herba showed very little inhibition of divisions of all cell lines. Conclusions: This experiment suggests that Orostachyos Herba has some anti-tumor effects on stomach and liver cancer cells. Progressive research on Orostachyos Herba and it's anti-tumor effects will be needed to determine its practicability as a cancer treatment.