서울의 PPNG 발생 빈도(1990)

김재홍,김한중,노영석,김영태,김중환,황규홍,이정덕,백승철,김건우,조상현,김준영,김석민,김영호,김상순,이승한,김방순,전덕규,하상근 대한화학요법학회 1992 대한화학요법학회지 Vol.10 No.2

The prevalence of PPNG among pretreated gonorrhoeae cases isolates at the VD clinic of Choong-ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1990, 162 strains of N.gonorrhoeae were isolated, among which 80 were PPNG. The prevalence of PPNG in Seoul showed increased tendency till 1987, thereafter, it has been stationary.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링

김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

생쥐모델에서의 망막모세포종 안구 자기공명분광법과 병리소견 : 초기경험

김동훈,김일중,양재한,김정훈,유영석,이봉재 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Purpose: To show various MR spectroscopy and correlated pathologic images of retinoblastoma in nude mouse with a new human retinoblastom cell line (SNUOT-Rbl) which innoculated into intravitreous cavity. Materials and Methods: The established cell line was innoculated into mtravitreous cavity of 14 eyeballs of 7 mice and a transplanted retinoblastoma was examed for 1 month. Tl-weighted image (T1WI), T2-weihgted image (T2WI), and MR spectroscopy were obtained with 1.5 Tesla MRI (Magnetom Vision, Siemens, Erlangen, Germany) and 4.7 Tesla MRI (Bruker, Medizintechnik GmbH, Karlsruhe-Ettlingen, Germany). After scanning, eyeballs were extracted and hematoxylin & eosin stained specimens were examined with the microscope. We compared MR spectroscopy with pathologic findings and evaluated characters of tumors. Results: Innoculated cells into eyeball of mice grew retinoblastoma (8/14, 57%). Eyeball with retinoblastoma protruded externally and showed focal hemorrhage. Tumors showed low (n=3), iso (n=4), high (n=1) signal intensity on TIWI, low (n=5), iso (n=1), high (n=2) signal intensity on T2WI, and well enhancement (n=8) with contrast, Involvement of optic nerve was suspected by MRI and confirmed by pathology (n=1). Conclusion: We could demonstrate MR spectroscopy of transplanted retinoblastoma by new tumor cell line in vivo. This imaging technique facilitate molecular imaging investigation of retinoblastoma.

상행 대동맥을 이환한 대동맥 벽내출혈과 대동맥 박리의 임상적 비교

김현숙,송재관,강덕현,김준,이철환,홍명기,김재중,박성욱,박승정 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

심장 이식 환자에서 발생한 Cryptococcus neoformans에 의한 뇌막염 2예 : The report of two cases

박재형,이윤정,강수진,정영훈,장진석,정준원,최상호,김순종,김양수,김재중 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.3

최근 장기 이식과 관련된 면역억제제의 사용 빈도의 증가 및 후천성면역결핍증 환자의 증가 등으로 인해 기회감염이 증가되고 있으며, C. neoformans에 의한 감염도 증가되고 있다. 저자들은 심장이식 후 6개월이상 지난 환자에서 발생한 크립토코쿠스 뇌막염 2예를 경험하였는데, 한 예의 경우는 거대세포 바이러스 감염과 함께 C. neoformans의 전신적 파급성 감염의 형태로 나타났었고, 폐에 A. fermigatus에 의한 감염이 같이 병발된 경우였다. 이들은 모두 경미한 증상만을 호소하였고, 뇌척수액 검사를 통해 진단받았으며 liposomal amphotericin B와 경구용 fluconazole을 사용하여 완치되었기에 문헌고찰과 함께 보고하는 바이다. Cryptococcal meningitis is a subacute or chronic mycotic infection caused by Cryptococcus neoformans and it is a second most common central nervous system (CNS) infection in organ transplant patients. The recent rise in incidence of the disease can be attributable to the wide use of immunosuppressive agents in organ transplant patients, the advent of acquired immunodeficiency syndrome (AIDS) patients and increased use of chemotherapeutic agents. We report two cases of cryptococcal meningitis after cardiac transplantation. Their symptoms were not typical and neurologic examination was nonfocal. One patient had disseminated cryptococcal infection and invasive pulmonary aspergillosis, it might be assodated with cytomegalovirus infection. They were diagnosed by cryptococcus latex agglutinin test and culture study with cerebrospinal fluid and treated with liposomal amphotericin B and oral fluconazole without any neurologic defects.

다제내성 그람양성균에 대한 Linezolid(Zyvox^(�))의 시험관내 항균력 비교

박대원,정희진,엄중식,황병연,김성범,이재갑,이연주,정혜원,정성주,박재형,이진수,손장욱,김우주,김민자,박승철 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

배경 : MRSA, VRE, VRSA같은 다제 내성 그람 양성균의 등장에 따라 glycopeptide를 대체할 새로운 항생제의 개발이 필요하게 되었고 결과적으로 새로운 항생제인 linezolid라는 항생제가 개발되었다. Linezolid는 이전의 항생제와는 다른 새로운 계열의 oxazolidinone으로 경구 이용률이 우수하다. 원내 및 원외감염의 중요한 원인균이 되고 있는 MRSA, VRE에 대한 적절한 경구용 항균제가 없는 국내에서 폐렴 및 피부 연조직 감염에서 경구용으로 사용해 볼 수 있는 약제이다. 본 연구에서는 고대 구로 병원에서 분리된 MRSA, VRE 등을 대상으로 다른 여러 항균제와 비교한 linezolid의 시험관내 항균력을 조사하고자 하였다. 재료 및 방법 : 연구대상은 1998년 1월부터 2000년 12월까지 본원에서 입원 및 외래를 통하여 피부 연조직 감염증 및 호흡기 감염증, 요로감염증으로 진단된 환자들의 가검물로부터 분리된 MRSA 60균주, VRE 43균주, PRSP 25균주를 액체배지 또는 한천배지 희석법을 통하여 linezolid 및 기타 항균제에 대한 최소발육억제농도를 구하였다. 결과 : 실험에 사용한 S. aureus는 모두 MRSA였고 이들은 linezolid에 대해 MIC_(90) 2㎍/㎖(MIC 범위 1-2㎍/㎖), Enterococcus spp는 모두 VRE로 linezolid의 MIC_(90)은 2㎍/㎖로 MIC 범위는 1-4㎍/㎖였다. 한 개의 균주에서 MIC 4㎍/㎖로 중등도 감수성을 보였으나 MIC breakpoint가 (8㎍/㎖인 내성균주는 없고 모두 감수성을 보였다. S. pneumoniae의 경우 penicillin 내성이었고, linezolid MIC_(90) 1㎍/㎖ (MIC 범위 0.5-1㎍/㎖)로 전부 감수성을 보였다. 결론 : Linezolid는 MRSA를 위시한 VRE, PRSP 등의 다제 내성 그람 양성균에 대하여 우수한 시험관내 항균력을 보임을 알 수 있었다. Background : The emergence of multi-drug resistant Gram-positive cocci, such as MRSA, VRE, and VRSA, necessitated to develop new antibiotics, which could replace the glycopeptide. As a result, a new antibiotics named linezolid was developed. Linezolid is different line of oxazolidinones with a good oral bioavailability, compared to other antibiotics. Since appropriate oral antibiotics are not presently available for MRSA, which is a major cause of nosocomial and community acquired infections, the introduction of linezolid will have favorable effect on treatment of infections such as pneumonia or skin infections. In this study, we investigated the antibiotic effect of linezolid on MRSA and VRE isolated from patients who were treated in Korea University Guro Hospital. Material and Methods : By using broth microdilution and agar dilution method we measured minimum inhibitory concentration (MIC) with sixty S. aureus, forty three Enterococcus spp., and twenty five S. pneumoniae isolates from patients who were diagnosed as skin, soft tissue, respiratory, and urinary infections in Korea University Guro Hospital from January, 1998 to December, 2002. Results : All of S. aureus used in this study were MRSA, and MIG_(90) of linezolid was below 2 ㎍/㎖ (MIC ranged between 1-2 ㎍/㎖). All of Enterococcus spp. were VRE, and had MIG_(90) of 2 ㎍/㎖ (MIC ranged between 1 to 4 ㎍/㎖). One of the VRE showed intermediate susceptibility with MIC of 4 ㎍/㎖. However, none was resistant with MIC breakpoint above 8 ㎍/㎖. All of S. pneumoniae were resistant to penicillin, but they were susceptible to linezolid with MICao of 1 ㎍/㎖(MIC range 0.5-1㎍/㎖). Conclusion : In conclusions, linezolid has an excellent in vitro antibiotic effect on multi-drug resistant Gram-positive cocci, such as MRSA, PRSP, and VRE.

성공적인 경피적 승모판 교련절개술 후 임상 경과와 재협착율

김영학,강덕현,송재관,최기준,이철환,홍명기,김재중,박성욱,박승정 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.2

자연 교과에서 地域 地質素材의 活用에 관하여 : 지묘동 일대 암석을 중심으로 focusing on the rocks in Chimyo-dong area

金在慶,金重旭 大邱敎育大學校 科學敎育硏究所 1989 과학·수학교육연구 Vol.13 No.-

The field geologic materials which are applicable to the geology unit in science textbook are investigated in Chimyo-dong area. It is found that this area offers several unique advantages in topography and geology which are as follows. First, this area is geographically located in the suburbs of Taegu and thus is easily accessible by bus. Secondly, the principal rock units in this area are composed of conglomerate, sandstone, mudstone, shale, basalt, granite, and gneiss etc. Thirdly, many outcrops are topographically exposed along the road cut and streams crossing the rock units. These outcrops are densely spotted to narrow area of Chimyo-dong and show large three-dimensional views of tops or bottoms freshly. And finally, geological features and data of each site are applicable to the learning subjects in teacher's guidebook for the science textbook. It is suggested that Chimyo-dong area is very suitable to field trip for further understanding of the geology units in science textbook of primary school.

중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정

김지훈,유소영,임선아,국중기,임상수,박슬희,황호길 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.2

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentisty, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P.endodontalis was found in 88.6% (39 / 54), P.gingivalis 52.3% (23 / 44), P.nigrescens 18.2%(8 / 44), P.intermedia 15.9% (7 / 44), B.forsythus 18.2% (8 / 44), A.actinomycetemcomitans 2.3% (1 / 44), T.denticola 25% (11 / 44) of the samples. The high prevalence of P. endodontalis and P.gingivalis suggests that they may play an important role in the etiology of endodontic infections.