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The fatty acid composition of peach seed oil was investigated. Peach seed oil was fractionated to neutral lipids, glycolipids and phospholipids, and each fraction was subjected to silicic acid column chromatography. The contents of neutral lipids, glycolipids and phospholipids in the crude oil were 83.3, 5.95 and 1.98%, respectively, and refined oil gave a trace amount of glycolipids and phospholipids. Unsaturated fatty acids found in the crude oil were linoleic acid (6.3%) and linolenic acid (1.9%) and the values were very close to those of the refined oil. Saturated fatty acid was abundant in the phopholipid fraction of refined oil.
Ribozymes (hammerhead and hairpin structures) which suppose to down-regulate GUS or CAT expression were constructed with the wound-inducible pin2K and constitutive CaMV 35S promoters. These ribozyme constructions have been successfully tested against their respective target sequences using in vitro transcription reactions. Transgenic plants were selected on MS medium supplemented with hygromycin B. After these plants flowered, crosses will be made to combine the ribozyme constructs with the following transgenes; 1) the wound-inducible CAT gene and the constitutive GUS gene 2) the wound-inducible GUS gene and the constitutive CAT gene 3) the constitutive CAT gene and the constitutive GUS gene. The level of expression of RNA will be correlated with the level of expression of the reporter protein. In addition, another ribozymes which may attack the viral RNAs (CMV and RBSDV RNAs) were synthesized and put in constructions containing CaMV 35S promoter and nos terminator. Sequence analysis are in progress to make sure the constructions. The resistance of transgenic plants against the viral infections will provide us the another way to develop the virus-resistant plants.
To understand the molecular structure and pathogenesis mechanism of garlic latent virus (GLV), it was purified by serial infection in Vicia faba which shows local necrotic spot by inoculation and amplified in Allium porrum L. which was considered to be a systemic host for GLV. Particle length of garlic viruses from mixed infected garlic plants ranged from 200 ㎚ to 2000 ㎚ by electron microscopy, but most of the particle was in the range of 600∼900 ㎚. Purified GLV particles showed 690 ㎚ long in average and their shape was slightly curved filamentous rod. Structural protein of garlic virus isolated from mixed-infected garlic leaves distributed between the range of MW 24∼38 kDa but GLV coat protein was 34 kDa. In the previous experiment, we have isolated several cDNA clones for garlic virus and classified these clones into 4 different groups on the basis of cross Southern hybridization. To identify cDNA clone for GLV, Northern blot analysis was carried out with 4 different cDNA clones as probes. The result showed that clone 28∼29 is a partial cDNA clone for GLV. The result of Northern blot analysis demonstrated that genome size of GLV is 8.2 kb and had poly (A) tail. Nucleotide sequence shows that a partial cDNA clones, 28∼9 and G8, have the length of 2140 bp.