서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Caffeine이 지구성 운동 수행능력에 미치는 영향과 억제성 아미노산에 의하여 활성화되는 이온통로의 조절작용

김은경,김영표,천병옥,이계영,김연정,임백빈,조영욱,김창주,김성수 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

To investigate the effect and mechanism of caffeine on endurance exercise, two experiments were performed. First, to test caffeine effect on aerobic exercise, 200-300g Sprague-Dawley rats were used and three groups, control group, low caffeine injection group and high caffeine injection group, were divided. Blood smpling by heart puncture were done at rest, after 30 min treadmill exercise, and after maximal exercise. Blood glucose, free fatty acid concentration were detected and following results were obtained. Glucose concentration showed significant difference between groups(p=0.0305) and also significant changes were exhibited between time(p=0.0004). Free fatty acid concentration had no difference between groups. but had significance between times(p=0.00065). Exercise endurance performance time showed significant difference(p=0.02350 in high caffeine injection group compared to control group. In this experiments, endurance exercise capacity was increased by caffeine injection. Therefore, second experiment was performed to investigate the effect of caffeine on ion current induced inhibitory amino acid neurotransmitter. GABA and glycine. Single periaqueductal gray neuron was acutely dissociated and nystatin perforated patch clamp was performed under voltage clamping condition. Caffeine evoked outward current in PAG neuron dose dependent manner. 1mM of caffeine application had no response. but 3mM caffeine evoked about 32.5±8.539pA outward ion current and 10mM caffeine evoked about 215.46±19.4pA outward current. 10^-2mM GABA activated Cl￣current and recorded by inward current. Caffeine inhibited GABA activated Cl￣ current concentration dependent manner. 10^-2mM of caffeine had no effect on 1-^-2mM of GABA response. but 10^-1mM caffeine inhibited GABA activated Cl￣ current about 5.74±2.13%, 1mM caffeine inhibited about 17.25±2.70%, 10mM caffeine inhibited GABA response about 45.31±7.71%. 10^-1mM of glycine activated Cl￣ current and also recorded by inward current. Caffeine inhibited glycine activated Cl￣ current concentration dependent manner. 10^-2mM caffeine decreased glycine activated Cl￣ current about 4.61±1.650%, 10^-1mM caffeine decreased about 6.49±2.24%, 1mM caffeine decreased about 26.82±4.27%, and 10mM caffeine decreased glycine response about 94.47±1.39%. These results suggest that caffeine inhibite inhibitory amino acid, GABA and glycine, this response causes excitation of CNS and this seems to be the basic mechanism of increasing effect to aerobic exercise performance by caffeine.

분편 인플루엔자백신(split influenza vaccine)의 임상효과 및 면역원성에 관한 연구

우흥정,김동림,정희진,천병철,이주연,안정배,김지희,박찬,신영규,김우주,김민자,박승철 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.1

목적 : 아단위 인플루엔자 백신 접종 후 백신의 인플루엔자 예방효과, 인플루엔자 방어 항체형성, 인플루엔자 백신의 안전성을 조사하고자 하였다. 방법 : 총 571명을 대상으로 인플루엔자 백신 접종을 하였고, 이들 접종자에서 인플루엔자 양질환의 이환을 조사하여 인플루엔자 백신의 인플루엔자예방효과를 알아보았고, 백신의 접종 전 및 접종 4주 후 혈청에서 혈구응집억제물(Hemagglutination Inhibition : HAI) 항체 검사를 실시하여 백신의 방어항체생성을 조사하였고, 백신의 안전성을 알아보기 위해 백신접종 후 1주일 이내의 부작용을 조사하였다. 결과 :백신 접종군과 백신 비접종군에서 인플루엔자 양 질환의 이환을 조사한 결과 접종군 28.35%, 비접종군 35.88%으로 나왔으며, p 값이 0.001로 통계적으로 접종군에서 유의하게 낮았고, 인플루엔자 양 질환의 예방 효과는 20.97%를 보였다. 백신의 방어항체 형성의 평가를 위해 유럽의 인플루엔자 백신 허가 기준을 조사하였는데 B/Guangdong/5/94균주의 백신접종 후 항체가 40이상의 비율을 제외한 다른 기준은 모두 만족 시켰다. 부작용은 전체 조사자 521명중 149명(29%)으로 주로 접종 부위의 국소 부작용을 호소했고, 전신 부작용은 2% 내외였으며 특별히 심각한 부작용은 발견되지 않았다. 결론 : 분편 인플루엔자 백신은 인플루엔자양질환의 예방과 방어항체생성에 효과 있으며 안전한 것으로 사료된다. Background : The safety and effectiveness of influenza vaccine are well known in developed country. The influenza vaccination has been recommended as one of the tentative immunization schedule for indicated persons since 1997 in Korea. But there are still no available data about them, even though nearly 5 million doses of influenza vaccine were used in 1997-1998 season. So it is immediately needed to investigate the safety. efficacy and immunogenicity of influenza vaccine among Korean. Methods : We studied the clinical efficacy of influenza vaccine by monitoring Occurrence of influenza-like illness in influenza risk group(vaccination ; 300, non-vaccination; 215) from December in 1997 to March in 1998. We used the split quadrivalent influenza vaccine containing 15 microgram of hemagglutinin of A/Beijing/262/95(HlNl), A/Wuhan/359/95(H3N2), B/Mie/1/93 and B/Guangdong/5/94. Hemagglutination inhibition(HA1) antibody titers were determined before immunization and 1 months after vaccination And we evaluated adverse effect of influenza vaccination at 7 days after vaccination. Results : Influenza vaccination was associated with si@icant reductions in influenza-like spptoms(vaccination group; 28.35%, non-vaccination group, 35.88%, p=0.001). The preventive effect of influenza-like i3lne.s among influenza risk goup was 20.97%. And immunogenicity of influenza A and B exceeded all of the European licensure criteria for immunogenicity except postvaccination proportion of titers 240 of B/Guangdong/5/94 strain. And the adverse effects were mainly local injection site problem and no serious adverse effect was noted. Conclusion : Split influenza vaccine is safe, inmunogenic and eff'tive in influenza risk group in Korea.

동종 골수이식 후 만성 이식편대숙주반응 환자에서 발생한 폐렴구균에 의한 수막뇌염 1예

신완식,김병욱,유진홍,김동집,김춘추,박종원,이종욱,김동욱,강문원,김양리 대한감염학회 1993 감염 Vol.25 No.3

Graft-versus-host disease (GVHD) is a frequent complication after bone marrow transplantation. Infectious complications are common in GVHD patients due to defect in cell-mediated immunity. A rare case of S. pneumoniae meningoencephalitis occured in a patient with extensive form of chronic GVHD after allogeneic bone marrow transplantation. He was immediately treated with full dosage of ceftriaxone and ampicillin. He suffered from various complications such as sepsis, acute renal failure, atelectasis, and seizure. Despite of aggressive treatment, he died probably due to renal shutdown and massive subacute cerebral infarction of left cerebral hemisphere. This report showed two unusual and rare features. First, the infection site was CNS rather than respiratory system. Second, the causative organism was S. pneumoniae, which is rare cause of CNS infection in immunocompromised patients.

Medicinal Chemistry : EVALUATION OF CADMIUM-INDUCED NEPHROTOXICITY USING URINARY METABOLOMIC PROFILES IN SPRAGUE-DAWLEY MALE RATS

( Yu Kyung Lee ),( Eun Young Park ),( Shi Won Kim ),( Ji Yeon Son ),( Tae Hyung Kim ),( Won Gu Kang ),( Tae Chun Jeong ),( Kyu Bong Kim ),( Seung Jun Kwack ),( Jae Won Lee ),( Suh Mann Kim ),( Byung M 영남대학교 약품개발연구소 2015 영남대학교 약품개발연구소 연구업적집 Vol.25 No.-

The aim of this study was to investigate urinary metabolomics profiles associated with cadmium (Cd)-induced nephrotoxicity and their potential mechanisms. Metabolomic profiles were measured by high-resolution 1H-nuclear magnetic resonance (NMR) spectroscopy in the urine of rats after oral exposure to CdCl2 (1, 5, or 25 mg/kg) for 6 wk. The spectral data were further analyzed by a multivariate analysis to identify specific urinary metabolites. Urinary excretion levels of protein biomarkers were also measured and CdCl2 accumulated dose-dependently in the kidney. High-dose (25 mg/kg) CdCl2 exposure significantly increased serum blood urea nitrogen (BUN), but serum creatinine (sCr) levels were unchanged. High-dose CdCl2 (25 mg/kg) exposure also significantly elevated protein-based urinary biomarkers including osteopontin, monocyte chemoattractant protein-1 (MCP-1), kidney injury molecules-1 (Kim-1), and selenium-bingding protein 1 (SBP1) in rat urine. Under these conditions, six urinary metabolites (citarate, serine, 3-hydroxyisovalerate, 4-hydroxyphentllactate, dimethylamine, and betaine) were involved in mitochondrial energy metabolism. In addition, a few number of amino acids such as glycine, glutamate, tyrosine, proline, or phentlalanine and carbohydrate (glucose) were altered in urine afrer CdCl2 exposure. In particular, the metabolites involved in the glutathione biosynthesis pathway, including cysteine, serine, methionine, and glutamate, were markedly decreased compared to the control. Thus, these metabolites are potential biomarkers for detection of Cd-induced nephrotoxicity. Our results further indicate that redox metabolomics pathways may be associated with Cd-mediated chronic kidney injury. These findings provide a biochemical pathway for better understanding of cellulat mechanism underlying Cd-induced renal injury in hunmans.

대구지역 백선 환자에서 분리된 Trichophyton속(屬)의 진균학적 성상 및 아형의 분류

김기홍,문병천,최종수 대한의진균학회 1997 대한의진균학회지 Vol.2 No.2

Background : Dermatophytosis has been investigated by many dermatologist, who has identified 9 species, specific in Korea among the 43 world-wide species. Tow isolates of Trichophyton(T.) genus common in Korea are Trichophyton(T.) rubrum and T. mentagrophytes. Th. verrucosum has been identified for the first time in Korea in 1986 and been increasing in number of reported cases. Objective : In this study, we try to classify the subtype of T. rubrum, T. mentagrophytes and T. verrucosum, as well as to identifiy the invasion of new so far, unidentified species with similar morphology. Method : Forty strains of T. rubrum, 40 strains of T. mentagrophytes and 30 strains T. verrucosum isolated from dermatophytosis patients in Taegu area, were examined for their characteristics, utilizing various media, hair perforation test and urease test. Resuts : T. rubrum was classified into 5 subtypes : granular type, portowine type, diffusable pigment producing type, yellow type and non-pigmented type, according to their color and surface morphology. Portowine type showed characteristic cottony surface with portowine color on the reverse side on gross morphology and microspcopically on rare occaion macroconidia and minimal tear-microconidia. Granular type showed granular surface with portowine collor on the reverse side, and many pencil-shaped macroconidia and abundant microconidia microscopically. Diffusable pigment-producing type showed similar findings, except for melanoid diffusible pigment on the Sabouraud media. Yellow type showed a cottony surface and yellow color on the reverse side. Microscipically, findings were the same to portowine type. Noncolored type showed no coloring on the reverse side; othervise same as those of portowine type. All strains were negative on hair perforation test. One of 40 stranins of T. rubrum showed typical gross and microscopic findings of T. rubrum, positive urease test and negative hair perforation test. All these findings suggest T. raubischeckii. This strains was identified for the first time in Korea. T. mentagrophytes was classified into 4 subtypes : pranular type, powdery type, purple-red type and cottony type, according to their color and surface morphology. Granular type showed fawn brown color with coarse granular surface grossly, and many club-shaped macroconodia and grape shaped microconidia but rare spiral hyphae microscopically. Powdery type showed cream to buff colored, fine powder surface, each colony being surrounded by mild cottony border and many club-shaped macrocondia, grape shaped microconidia and spiral hyphae microscopically. Red-purple type showed characteristic red-purple color with cottony and powdery surface, and microconidia and spiral hyphae microscopically. Cottony type showed abundant whitish cottony surface and many round microconidia microscopically. T. verrucosum showed a nery slowly growing, mountain-like appearance with shallow valley and narrow or wider skirt. Microspcopically, sring-bean shaped macroconidia, a few microconidia, and many chlamydoconidia were present. All strains showed positive hair perforation. Nineteen of 30 strains showed negative urease test. All strains grew on Trichophyton media Ⅲ. Twenty(65.4%) of 31 strains grew on Trichophyton media Ⅳ. Conclusion : These findings suggest that all dermatophytes should be examined thoroughly to investigate any strain that may be brought in from foreign countries.

여가스포츠 참여 직장인의 성격유형과 여가만족간의 관계

전병관,김홍석 慶熙大學校 社會體育硏究所 1998 體育科學論叢 Vol.- No.11

This study was aimed at defining on what relationship have between personality styles and leisure satisfaction of the workers. In order to accomplish such purpose, studying issues were established as indicated below. First, Dependent one among personality styles of workers participating in leisure sports was showed to the strong relationship at aesthetic leisure satisfaction. Second, Independent one among personality styles of workers participating in leisure sports was showed to the strong relationship at educational leisure satisfaction. Third, Dependent one among personality styles of workers participating in leisure sports was showed to the strong relationships at rest leisure satisfaction. Froth, Independent one among personality styles of workers participating in leisure sports was showen to the strong relationships at psychologial leisure satisfaction. Fifth, Dependent one among personality styles of workers participating in leisure sports was showed to the strong relationships at physical leisure satisfaction. Sixth, Independent one among personality styles of workers participating in leisure sports was showen to the strong relationship at social leisure satisfaction.

PbTiO₃강유전체 박막의 유전율 특성

김창석,정천옥,김병인 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.2

This paper evaporates PbTiO_(3) layer thickness differently with RF sputtering method on Si Wafer(n-100). This study is performed to examine the characteristics of electric constant, photon energy and dielectric loss according to the thickness of PbTiO_(3) and increase the reliability and reproduction of PbTiO_(3) thin film. It is confirmed that the variation of electric constant by frequency resulted from the formation of particles within thin film, the particle size and the polarization on grain boundary. In conclusion dielectric loss depends on the value of imaginary number (ε_(2)), polarizability falls zero(0) by resonance absorption and is close to 0 of tanδ value and Q value of dielectric loss.

텔레비전 브라운관 생산업체 남자 근거리 검사작업자에서 시력, 눈물분비기능의 변화

김성아,천병렬,김상우,정상재,이광자 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : 장기간의 근거리검사작업이 굴절력 및 눈물분비량의 감소를 초래하는지를 관찰하고자 3년간추적 연구를 시행하였다. 방법 : 구미소재 일개 TV브라운관 제조공작의 미세한 이상여부를 확인하는 검사작업에 종사하는 남자 근로자 396명 중 정시안, 눈물분비기능이 정상, 40세 미만이며 3년간 추적가능했던 65명을 대상으로 하였다. 시력, 색신 검사 외에 자동굴절력검사, 누액분비검사, 안증상, 근무기간, 실제 검사작업을 수행하는 시간, 일상생활중 TV시청 및 VDT사용시간,작업환경에 대한 불편감, 안경착용여부 등에 대한 설문조사를 시행하였다. 이중 누액분비검사는 쉬르머 I검사로써 1996년과 1998년에만 실시되었으며, 시력및 누액분비기능의 변화가 안증상과 관련이 있는지를 보기 위한 설문조사는 1999년에 시행되었다. 결과 : 32개월간의 구면굴절력의 변화량은 좌우안모두 0.18D(diopter)였으며, 년간 변화량은 0.068D/yr로서 근시화되는 방향으로 진행하였다 20개월간의 눈물의 변화량은 좌안이 6.57 mm/s min, 우안이 6.40 mm/5 min만큼 감소하였다. 30세 이하 연령군과 2년 미만 근무군에서 0.5 D 이상 감소한 빈도가 높았으나 유의한 차이는 없었으며, 조명, 소음,공기에 대한 주관적인 상태와 작업시간외 영상화면사용시간에 따른 차이도 없었다 안불편감의 호소율은 52.3 %이었고 굴절력의 변화, 나안시력의 변화, 눈물분비변화량에 따른 차이는 없었다. 연령, 근무기간과도 관계가 없었고, 조명, 공기, 소음에 대한주관적인 상태에 따른 차이도 없었으나 작업시간외영상화면사용시간이 4시간 이상에서 4시간 미만에 비해 유의하게 높았다. 구면굴절력, 나안시력 및 눈물분비 변화량, 20개월 후의 눈물분비상태, 나이,근무기간, 불량한 조명, 일상생활중 VDT 혹은 TV시청을 독립변수로 하고 안불편감여부를 종속변수로하여 로지스틱 회귀분석을 실시하였다. 유의한 변수는 작업시간외 영상화면의 사용시간으로 4시간 이상인 군이 4시간 미만인 군이 비해 비차비가 10.5(95% 신뢰구간 1. S~73.9)이었다. 결론 : 근거리 검사작업자에서 굴절력 및 눈물분비의 저하가 관찰되었으며, 그 방향은 근시화 및 건성안의 진행방향이었으나, 눈물분비의 저하는 유의한 정도는 아니었고, 근거리검사작업으로 인한 부가적인 근시화의 영향은 관찰되지 않았다. 근거리작업자의 안건강관리측면에서 시력뿐만 아니라, 눈물분비기능, 눈물층의 상태 등을 포함한 안건강프로그램이 필요하다. Objectives : The aim of this field-based longitudinal study was to investigate the effects of near-work on lacrimation and visual function, particularly refractive power. Methods : Our study tracked sixty five male workers for about three years by means of ophthalmologic examinations and questionnaires, to collect data including eye symptoms and subjective work environmental factors. Ophthalmic examinations included visual acuity, Shirmer I test, and autorefractometry. In the first year, the inclusion criteria were ages less than 40 years, no history of ophthalmic diseases, emmetropia (-1.0 ≤spherical refractive power〈 1.0) and normal lacrimation. Results : Over a period of 32 months, spherical equivalents were decreased as much as 0.18D (diopter) for LE(left eye) and 0.18D for RE (right eye). Lacrimation by Shirmer's I test during 20 months were decreased as much as 6.57 mm/5min for LE, and 6.40 mm/5min for RE. Although more myopic-changed workers demonstrated young age (〈30 years) and short work duration (〈2 years), there was no significance to this correlation. Additionally, subjective work environmental factors such as poor illumination, noise, uncomfortable air and daily use of VDT and/or TV were shown to have no significant effect. However, as for eye discomfort, which was reconstructed from several dry eye symptoms, the daily use of VBT and/or TV was a significant variable. According to a logistic regression analysis concerning the effects of refractive power, lacrimation, work duration, poor illumination, and daily use of VDT and/or TV on eye discomfort, only daily use of VDT and/or TV greater than 4 hours displayed OR of 10.5. Conclusions : Myopic changes due to near work were not observed, however lacrimal function was changed in workers. These results suggest that we should consider various factors such as lacrimation, tear film and refraction as well as visual acuity in order to establish an eye care program among near-work performing employees.

感冒辨證에서 희수식 맥진기를 이용한 肺脈觀察의 臨床的 意義

김준명,고재찬,배한호,박양춘,김병탁,김철중 대전대학교 한방병원 2001 惠和醫學 Vol.10 No.1

By useing hisusik-makjingi(희수식맥진기) we analysed lung-pulse of common cold patient. The result as fellow ; 1. In case of wind-cold common cold patient, It is short-avove pulse type and when cold transfer to fever it is dry-cold type pulse. 2. In a case of wind-heat, there is a pulse of above-frequent or frequent-stringed. And also we can find out that in a mild fever case there is not a frequent pulse meaning the heat. 3. In case of vutal energy shortage and yabggi deficiency, we can also notice that the pulse is so powerless. 4. The patient who have had an old complaint and a serious illness didn't show the accordance with the pulse and common cold type.