Breeding of Tetraploid in Codonopsis lanceolata (Sieb. et Zucc.) Trautvetter by Colchicine Treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

황색포도구균의 균혈증에서 초기 항생제 치료가 예후에 미치는 영향

김성한,박완범,이기덕,강철인,최영주,김홍빈,박상원,김의석,오명돈,김의종,최강원 대한감염학회 2002 감염 Vol.34 No.5

Breeding of Tetraploid in Platycodon grandiflorum (Jacq.)A. DC. by Colchicine treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Platycodon grandiflorum in Campanulaceae. The most successful colchicine treatment for tetraploid production in P. grandiflorum was soaking treatment using 0.01 and 0.5% colchicine solution for 1 hour and 12 hours, respectively. Morphological characteristics of both diploid and tetraploid were similar, but tetraploid plants had more leaves. Compared to diploid, tetraploid had the larger stomata, but less number of stomata. Fresh weight of tetraploids was 20∼40% heavier than that of diploid.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

Lutembacher 증후군 환자 증례 보고

김성범,서정주,곽철훈,김상민,이보라,민선경,황은구,김용인,조욱현,최석구 인제대학교 2008 仁濟醫學 Vol.29 No.-

Lutembacher's syndrome is rare combination of mitral stenosis (MS) and atrial septal defect (ASD). The hemodynamic interplay between the MS and ASD leads to wide variation of clinical presentation. Here we describe a 43-year-old female with Lutembacher's syndrome and tricuspid regurgitation with pulmonary hypertension, who underwent direct closure of ASD and tricuspid valvuloplasty successfully. We also reviewed other literatures in an effort to increase awareness of this condition.

PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

맞춤형 취업지도 방안 연구 : 순천향대학교 사회과학대학 졸업생들을 중심으로

김현철,김홍진,윤주명,김재필 순천향대학교 사회과학연구소 2007 순천향 사회과학연구 Vol.13 No.1

The purpose of this study was to develop a foundation for planning a customized job guidance system for social science major students at Soonchunhyang University, In order to suggest a practical implication related to the student support system, the recent graduates of the College of Social Science at Soon chunhyang University were surveyed. A total of 110 graduates representing all majors of this college, Participated in this study. According to the empirical results, it appeared that a college-wide strong job search guidance system which are structured focusing on sharpening foreign language and interview skills should be initiated. The implications of the result are discussed to construct a customized career planning guidance svstem for social science majors at Soonchunhyang University.

발산풍열약(發散風熱藥)의 항고혈압작용에 관한 연구

金護哲,朴珠瑛,安德均 대한본초학회 1999 大韓本草學會誌 Vol.14 No.1

Balsanpungyeolyak, Expelling Pathogenic Wind-Heat Drug, have been used to treat the symptoms such as fever, headache, neck stiffness and skin disturbance which are commonly shown in systemic fever disease like influenza. We thought that the symptoms from the actions of wind and heat in Traditional Korean Medicine are similar to the ones of hypertensive patients in clinic. We chose five drugs among expelling pathogenic wind-heat drugs, Puerariae Radix, Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus, to investigate their antihypertensive effects on S.H.R. and normotensive S.D rats, direct relaxant activity on isolated rabbit aorta, and ACE inhibitory activity. Menthae Herba and Arctii Fructus decreased blood pressure significantly in SHR, but not in normotensive rats, Direct vasodilatative effects on isolated rabbit aorta was not observed in any group. Chrysanthemi Flos, Menthae Herba, Mori Folium, and Arctii Fructus inhibited the actions of ACE dose-dependently. Therefore it is concluded that expelling pathogenic wind-heat drugs has not direct correlation with antihypertensive activity. However, it can be suspected that some drugs for dispersing the actions of wind and heat may regulate blood pressure through ACE inhibition and further study will be helpful to understand the mechanism.

구치부 복합레진의 인장강도에 관한 실험적 연구

김재곤,이용희,양철희,백병주 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.3

1960년대 수복재로서 복합레진이 소개된 이래, 심미성이 우수하고 치질에 대한 접착성이 있는 복합레진이 전치부 수복용 재료로서 널리 사용되어 왔으며, 최근 레진과 필러의 개선에 의해 기계적 성질 내마모성 등의 물리적 성질이 크게 증가하면서 구치부 수복용 재료로서도 점차 그의 응용이 확대되고 있지만. 높은 음력이 작용하는 구치부의 경우 그 사용이 제한적이고 임상적인 예후가 불확실하다는 단점이 있어왔다. 초기의 구치용 복합레진의 예후의 보고에 의하면 비교적 초기에 변연파절이 발현하고 있는 듯하다. 이런 원인으로써는 재료 자신의 강도 접착기법,환경요인등 여러 가지 물성이 복잡하게 영향하고 있다고 생각되어지기 때문에 간단히는 설명할 수 없지만, 일반적으로 취성재료가 인장응력으로 파단하는 것을 생각하면 구치용 복합레진의 인장강도에 대해서 상세히 검사할 필요가 있다고 생각한다. 본 연구에서는 구치부 수복용 복합레진의 인장강도를 평가하기 위해. 5종의 광중합 복합레진(Denfil(Vericom, Korea), P6O(3M,U.S.A.), Unifil S(GC, Japan), ZIOO(3M, U.S.A.), Z250(3M, U.S.A.))을 시험재료로 선택하여 소형인장시험편을 제작한 다음 thermal cycyling과 시효처리를 시행한 후 직접 인장시험법에 의해 인장강도를 측정하고 다음과 같은 결론을 얻었다 1.P6O의 인장강도는 타 실험군인 Denfil, Unifil S, Z1OO에 비해 유의성 있게 높은 수치를 보였다(p<0.05). 그러나 Z25O과는 유의한 차이를 보이지 않았다(p>0.05). 2.Denfil은 타 실험군인 P6O, Unial S, ZIOO. Z25O 모두에 비해 유의성 있게 낮은 수치를 보였다(p<0.05) 3.Unifil S와 Z100은 P6O과 Z250보다 낮은 수치를 나타냈으나(p<0.05). Denfil보다는 증가된 수치를 나타냈다. (p<0.05): Unifil S와 Z100간의 유의한 차이는 없었다(p<0.05). The purpose of this study was to evaluate the tensile strength of light-cured restorative posterior resin-based composites. Five commercially available light-cured composites(Denfil : DF, P6O : PS, Unifil S : US, Z100 : ZH, Z250 : ZT) were used. Rectangular tension test specimens were fabricated in a teflon mold giving 5mm in gauge length and 2mm in thickness. Specimens were subjected to the 5,000 thermal cycles between 5℃ and 55℃ and the immersion time in each bath was 15 second per cycle. Tensile testing was carried out with Instron at a crosshead speed of 0.5mn/min and fractured surface were observed with scanning electron microscope. The obtained results were summarized as follows ; 1. The tensile strength of PS was highest. PS was significantly higher than DF, US and ZH(p<0.05). but in the case of ZT was similar to PS(p>0.05) . 2. The tensile strength of DF was lowest. DF was signigicantly lower· than PS, US, ZH and ZT(p<0.05) . 3. The tensile strength of US and ZH were significantly lower than PS and ZT(p<0.05) but were significantly. higher than DF(p<0.05) . The tensile strength of US and ZH were similar(p>0.05).

어린이의 식이습관과 치아우식발생과의 상관관계에 관한 연구

김재곤,전철완,이두철,백병주 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

본 연구는 치아우식 발생과 식이습관과의 상관관계를 알아보기 위하여 전주시내 소재 유치원에 다니는 남아 143명과 여아 124명을 대상으로 유치의 우식경험지수를 조사하고, 어머니로부터 설문조사를 통하여 어머니의 직업유무, 수유경험 및 어린이의 식품기호도 등을 조사한 후 이상의 자료를 분석하였으며, 본 연구에서 나타난 결과들을 중심으로 다음과 같은 결론을 얻었다. 전체 어린이의 유치 우식경험지수는 2.92±1.05이였으며 여아(3.40±1.46)가 남아(2.51±0.97) 보다 다소 높은 우식경험지수를 나타냈으나, 남녀간의 유의한 차이는 나타나지 않았으며(P>0.05), 연령에 따른 유치의 우식경험지수를 비교한 결과, 나이가 증가할수록 높아지는 양상을 보였으며, 3세와 4세에 비해 5세와 6세에서 각각 높은 우식경험지수를 나타냈다(P<0.05). 각 식품군에 대한 기호도에서 남아는 과실류를, 여아는 해조류를 가장 좋아하였으며, 해조류를 제외한 다른 식품군에서 남녀별 유의한 차이를 나타내지 않았으며(P>0.07), 조사 식품군에 대한 어린이의 기호도와 관련한 유치 우식경험지수를 비교한 결과. 당류(r=0.3854, P<005), 유류(r=0.4328, P<0.05), 음료(r=0.2586, P<0.05)에서는 유의성이 인정되었으나, 다른 식품군에서는 유의한 상관성이 인정되지 않았다(P>0.05). The aim of the present study was to analyze relationship between dietary habits and dental caries experience inpreschool children. All 143 boys and 124 girls living in a chonju city were investigated dmf index by dentists through oral examination and dietary habit by children's mothers through questionaires such as infant diets and food preferences. The statistical analysis were performed to evaluate whether variations in dietary habits are related to dental caries experience. The obtained results were as follows; 1. The average dmf index scores of all children were examined 2.92±1.05. The girls had higher than the boys, but no significant differences between the genders were seen the을 index(P>0.05). 2. The higher were their age of children, the higher dmf index was investigated. The dmf index of 5 and 6-yr old childrens was higher than that in 3 and 4-yr old children(P<0.05). 3. No sigificant difference was found between the present of mother's occupation (P>0.05) and type of infant dietary habits(P>0.05). 4. The fruits and sea weeds were showed high degree of preference and fats and vegetables were showed low degree generally. Among 13 food groups, there was no significant difference between boys and girls except for sea weeds(P>0.05). 5. Among 13 food groups, the을 index was positively associated with sugars(r=0.3854, P<0.05), dairy products(r=0.4328, P<0.05) and soft drinks(r=0.2586, P<0.05), and negatively associated with other food groups.