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연속 2 단계 교반조 효소 반응장치의 최적화에 관한 연구
박영훈,유두영 한국화학공학회 1978 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.16 No.4
For production of 6-aminopenicillanic acid by enzymatic hydrolysis of benzylpenicillin, two-stage continuous stirred tank enzyme reactor system was evaluated. Using proper mathematical model of the reaction kinetics and kinetic constants of soluble and immobilized forms of penicillin amidohydrolase, the reactor system was simulated with an aid of a computer and a set of optimum operating conditions was found. In comparison with the single stage system, the productivity of the two-stage enzyme reactor system was 50% higher than that of the single stage system under the same operating conditions. For the industrial applications, the reactor system using immobilized penicillin amidohydrolase was found to be far more advantageous in terms of productivity than that using soluble enzyme because of the reduced inhibitory effect of the product, 6-aminopenicillanic acid, on the immobilized enzyme as compared to the same effect on the soluble enzyme.
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급성 A형 간염의 합병증으로 발생한 혈구포식증후군 1예
박영훈,김대영,김성두,이정희,이제환,이규형 대한내과학회 2011 대한내과학회지 Vol.80 No.-
Virus-associated hemophagocytic syndrome (VAHS) is a severe hematological disease characterized by persistent fever, peripheral blood cytopenia, hyperferritinemia, hypertriglyceridemia, and hemophagocytosis in the bone marrow, spleen, or lymph nodes. Despite the high incidence of viral infections in the general population, VAHS complicated by hepatitis A infection is rare worldwide, and there is no standard treatment for VAHS. Treatments include correcting the peripheral blood cytopenia and controlling the infectious causes and immunosuppression, such as with cyclosporine A and corticosteroids. We report on a patient who was diagnosed with hepatitis A virus-associated VAHS, and has been free of signs and symptoms of VAHS for 2 years with a combination of corticosteroid and cyclosporin A. (Korean J Med 2011;80:S278-S282) A형 간염바이러스에 유발된 혈구포식증후군은 드물지만, 발생할 경우 심각한 임상경과를 보인다. 본 저자들은 A형 간염바이러스에 의해 유발된 혈구포식증후군에 대한 치료와 2년간의 추적관찰의 결과를 보고한다. 이전 특이병력이 없었던 24세 여자 환자로 급성 A형 간염으로 진단받고 보존적 치료 중 간 기능 악화 및 수혈에 교정되지 않는 빈혈 그리고 지속적인 발열의 증상으로 시행한 검사실 및 골수검사상 혈구포식증후군으로 진단되었다. 진단 직후 투여한 덱사메타손 단독요법에 반응이 없어 시클로스포린과 덱사메타손 병합요법을 시행하였고, 이후 증상 및 검사실 소견이 호전되어 치료 시작 한 달 후 퇴원하였다. 이후 특이 증상을 호소하지 않고 있으며, 치료 시작 2년째 면역억제제의 복용 없이 경과관찰 중이다.
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IR 이미지와 PDE 해석에 의한 결함깊이 하이브리드 연구
박영훈,양성모 한국기계기술학회 2015 한국기계기술학회지 Vol.17 No.4
This study is a duralumin and applying heat to the STS-wide measurement range is used, the surface temperature by using the temperature of studying the non-destructive testing of a new paradigm to estimate the position and size of a structure defect purpose. STS and duralumin which has a structure defect is applied a heat by a heater. Its difference of STS and duralumin surface temperature is measured using IR thermography. The estimated result of the STS and duralumin experiment and that of theoretical analysis of PDE are compared and analyzed to diagnose the STS and duralumin defect. Moreover, this study can save time and money and improve accuracy contrast to the existing ultrasonic NDE experiment. In addition, the new paradigm of NDT/NDE by reverse-engineering is going to be valid if the data of thermal analysis and temperature distribution from the specifications of many materials is accumulated and verified. In this study, the average surface temperature of the STS in the same heating condition was 4.53 ° K higher than that of duralumin and both of the surface temperature showed an inflection point in the defect 2.5mm.The maximum surface temperature difference was formed on the 2.5mm, and the study proves its reliability because the average surface temperature of the STS and duralumin was 0.74°K, 0.45°K higher than the theoretical surface temperature.
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박영훈,이용재,최영환,손병구,강점순 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5
PCR performance of different primer sets that are used for detecting Acidovorax avenae subsp. citrulli from bacterial fruit blotch (BFB)-infected plant tissue and seeds in watermelon were evaluated. PCR specificity and sensitivity in detecting Acidovorax avenae subsp. citrulli were examined for four BFB-PCR primer sets. From screening of 10 different bacterial species and three subspecies of Acidovorax avenae, the best PCR-specificity to A. avenae subsp. citrulli was observed from RST 49/51 after digestion of the PCR products with Sau 96I. PCR sensitivity was evaluated by using serial dilutions of template DNA samples achieved from BFB-infected watermelon seeds and leaf tissues, and the highest detection sensitivity was observed from Seminis WFB 1/2. To improve PCR specificity to A. avenae subsp. citrulli of Seminis WFB 1/2, PCR amplicons from five bacterial species were sequenced and a new CAPS marker was developed based on the restriction enzyme site polymorphisms unique to A. avenae subsp. citrulli. CAPS marker uses primer sequence of Seminis WFB 1/2 of high detection sensitivity, and discriminates A. avenae subsp. citrulli from any other bacterial species.
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Approximate Analysis Method for Composite Beams with Partial Interaction Using Fourier Series
박영훈,김성훈,이승용,최준혁 한국강구조학회 2013 International Journal of Steel Structures Vol.13 No.2
This paper presents an alternate analysis method for calculating the deflection and internal forces for arbitrary boundary and loading conditions of composite beams with partial interaction. The internal axial force was approximated by the Fourier series to solve the governing equation of composite beam. Then, the coefficients of the Fourier series were determined using the Euler-Lagrange equation of minimizing a definite integral. An evaluation of the convergence and the accuracy for the proposed method in the determination of the deflection for four types of boundary conditions with four types of loading cases was made. The results were agreed with those obtained by other analytical methods. The displacement of the partial composite beam depends on the stiffness of shear connectors, boundary conditions and loading conditions.
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