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안주희,V.M. Balasubramaniam 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.2
The effects of temperature heating-up rate and pressure building~up phase on the inactivation of Listeria innocua ATCC 33090 were evaluated in buffered peptone water. The number of 1. znnocua was reduced by 5.57 and 6.52 log CFUI mL during the nonisothermal treatment (the come-up time followed by isothermal process) and i?e Isothermal treatme~t, res ectivel at 60°C. When compared to the isothermal treatment (0.76<D value<0.9?), 1. znn~cua. exposed at t e no~sothe~al treatment (1.58<D value<2.31) became more resistant to heat. The come-up tune r~ductIons m numbers of 1. innocua significantly increased with increasing the heating rates (p<0.05). The pronounced ~eductI?n was observed by more than 5 log CFU/mL at 33.2°C/min of temperature heat!ng-~ate. T.he effect of the combmed high pressure and thermal processing on the inactivation of 1. innocua increased WIth mcreas~g pressure and temperature. ~t all temperature levels from 40 to 60°C under 700 MPa,1. innocua was not detected by ennchment culture (>7 log reduction).
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안주희,김송화,상미경,진용주,김다연,김상윤,송재경 한국미생물학회 2022 미생물학회지 Vol.58 No.4
Pseudomonas moraviensis strain EFBE32 is a biocontrol bacterium against bacterial wilt which is known as most destructive disease caused by Ralstonia solanacearum. Here, we report the whole genome sequence of P. moraviensis strain EFBE32. The sequence analysis revealed that P. moraviensis strain EFBE32 has a single 6,030,129 bps circular chromosome with a DNA G + C-content of 60.1%. This chromosome contains 5,239 coding sequences and 16 rRNA and 69 tRNA genes. In the result of sequence analysis, it is revealed that strain EFBE32 possessed genes coding the disease suppression related enzymes, acyl-homoserine lactone acylase (QuiP and PvdQ) which is known as quorum quenching enzyme, and hydrogen cyanide synthase (HcnA, B, and C).
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연료전지용 술폰화 폴리아릴렌에테르술폰 랜덤공중합체 강화복합막의 제조 및 특성
안주희,이창현 한국막학회 2016 멤브레인 Vol.26 No.2
Sulfonated poly (arylene ether sulfone) (SPAES) random copolymers have merits such as high proton conductivity, relatively low production cost, and thermochemical resistance when applied as polymer electrolyte membranes for fuel cells. However, it is difficult to directly employ SPAES copolymers into practical fuel cell membrane applications owing to their low chemical stability and dimensional instability under harsh operation conditions. A plausible solution is to impregnate SPAES copolymers into support films (e.g., electrospun polyimide support) with interconnected pore structures and high thermochemical toughness. In this study, a SPAES copolymer with a swivel group, which induces high free volume for fast ion transport, is chosen as ionomers to prepare pore-filling membranes (PFMs). The feasibility of the resulting membranes is evaluated via membrane characterizations.
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안주희,조강현 한국작물학회 2009 한국작물학회지 Vol.54 No.2
Pollen flow is one of the essential components in the ecological risk assessment of transgenic crops, because pollen can act as a vehicle to disseminate transferred alien genes. Pollen flow pattern of a cultivated rice variety and Living modified (LM) rice was studied at diurnal and distance changes under field. We measured airborne pollen density at the distances of -1, 0.5, 0, 1, 2, 3, 4, 5, 7, 9, 11 and 13 m from rice cultivation and recorded the direction and speed of wind using weather station in the conventional rice paddy field during the flowering period of rice. Diurnal changes in pollen density were observed as a peak between 10:00 to 13:00 hr. The density of airborne rice pollen geometrically decreased with the increase of distance from pollen sources. It is therefore necessary to carry out a detailed investigation of pollen flow of a particular species, where ecological risk assessment requires an accurate estimation of pollen flow including both distance and intensity of pollen dispersal. The rice pollen flow was significantly influenced by weather conditions, particularly by wind direction and speed. The precise determination of the local wind conditions at flowering time therefore appears to be of primary importance for setting up suitable isolation distance from transgenic rice in the field.
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축산 폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화
안주희,김성수,김태호,이준엽,오상집,이진하,이현용 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
돈분 폐수의 효율적인 처리를 위해 6 W/㎡, 12 W/㎡와 24 W/㎡의 조도 하에서 온도를 15℃에서 40℃까지 각각 변화시켜, 광합성 미세 조류인 Spirulina platensis의 생육에 관한 최적 생육 조건 결정에 관한 실험을 하였다. 균체의 비생육속도는 30℃까지는 온도 증가에 따라 증가하였다. 균체의 생육에 있어서 온도의 영향을 설명하기 위해서 Arrhenius 식을 이용하여서 얻은 활성화 에너지 값은 13.5kal/mol이었다. 순수 배지에서 배양된 균체의 비생육속도가 0.31(1/day)인 반면 30% 돈분 첨가 배양시 얻은 균체의 비생육속도는 0.24(1/day)였다. 폐수내의 총 질소량과 이의 제거속도로 균체의 폐수 처리 능력을 평가할 수 있었다. 돈분 농도를 달리 첨가한 회분배양에 있어서 70~93%(PO_4^3- -P), 67~93%(inorganic nitrogen), 80~90% (COD) 그리고 37~56%(organic nitrogen)의 감소율을 보였다. 총-질소와 총-인의 1차반응 감소 속도 상수는 각각 0.17(1/day)와 0.14(1/day)로 계산되었다. 20% 돈분을 첨가하는 연속배양에 있어서 회석률이 0.20(1/day)일 때 최대 균체농도가 1.52(g/L)를 유지했다. 돈분처리 후 얻은 균체의 성분은 단백질이 58.7%, 지방 11.0% 그리고 회분이 15.6%였다. The kinetics of growing microalga, Spirulina platensis was investigated to treat swine wastes with optimum growth conditions. Temperature was varied from 15 to 40℃ at three different light intensities, 6 W/㎡, 12 W/㎡ and 24 W/㎡. The specific growth rate was increased as temperature increased up to 30℃. The activation energy was estimated as 13.5 kcal/mol by an Arrhenius relationship. 0.24(1/day) of specific growth rate was obtained from batch cultivation with 30% swine wastes, compared to 0.31 (1/day) from clean culture. It was found that Spirulina platensis was able to reduce 70~93% of PO_4^3- -P, 67~93% of inorganic nitrogen, 80~90% of COD and 37~56% organic nitrogen by adding various concentrations of swine wastes for 12 days of batch cultivation. Rate constants for removing nitrates and phosphates in treating swine wastes were estimated as 0.17 (1/day) and 0.14 (1/day) in the first order reaction, respectively. 1.52(g/L) of maximum cell density was maintained at 0.20(1/day) of dilution rate in continuous culture, adding 20% swine wastes for 30 days. The chemical composition of the biomass obtained from the process showed 58.7% of protein, 11.0% of lipid and 15.6% of ash.
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