Development of a bivalent live vaccine against duck hepatitis A virus types 1 & 3 and vaccine program for breeder ducks in Korea

Jae?Hee Roh(노재희),Min Kang,Bai Wei,Se-Yeoun Cha,Jeong-Gon Cho,Hyung-Kwan Jang 한국가금학회 2017 한국가금학회 정기총회 및 학술발표회 Vol.2017 No.11

어린 오리에 치명적인 질병을 일으키는 오리바이러스성간염의 예방을 위해 혼합생백신 개발, 백신의 방어기전 연구 및 종오리백신 프로그램의 개발로 질병방어 및 제어를 목표로 연구를 진행하였다. 첫 번째, 국내에는 1형과 3형이 동시 유행하고 있으며, 최근에는 3형의 발생보고가 증가하는 추세이다. 특히 1형과 3형의 복합감염사례도 빈번히 발생하고 있다. 이런 상황에서 효율적인 질병관리를 위해 국내에 발생하는 오리간염 1형과 3형을 동시에 방어하는 혼합백신을 개발하고 그 효능을 확인하였으며, 운반 및 보관 용이성 향상을 위한 연구를 진행하였다. 두 번째, 오리감염 생백신의 3-5일 만에 조기면역을 획득하는 특성분석을 위해 세포성 및 체액성 면역반응을 평가하였다. Cytokine이 백신접종 그룹에서 증가하는 것을 확인하였고, 특히 IFN-α에서 강한 증가를 보였다. 또한 항체가 평가에서는 기존에 알려진 바와는 다르게 백신 후 3일째에 항체를 획득하였고, 10일째에는 최고수준의 항체가 수준을 나타냈다. 세 번째, 1주령이하의 어린 일령에 치명적인 질병의 특성상 모체이행항체에 의한 예방이 가장 효과적이라는 점에 착안하여 종오리백신 프로그램을 개발하고 농장에 적용하여 평가하였다. 종오리 농장 4개동 4천수의 종오리를 대상으로 백신을 실시하였으며, 모체의중화항체가(VN titer) 8 log2이상에서 후대병아리 14일령까지 100% 방어효능을 확인하였다. 모체이행항체의 전달률은 11%수준으로 나타났으며, 후대병아리의 항체가를 개체별 추적하여 계산한 결과 모체이행항체 반감기는 3-5일령임을 확인하였다. 본 연구에서는 오리에 치명적인 질병을 일으키는 오리바이러스성간염 예방을 위한 혼합생백신 개발에 관한 연구로 기존의 국내 상용화백신의 한계점을 극복하고, 국내에 유행하는 혈청형들을 단회백신으로 효과적으로 방어할 수 있는 혼합생백신을 개발하였으며, 백신의 특성상 조기면역에 대한 세포성 및 체액성 면역기전을 평가하였다. 또한 종오리백신 프로그램을 국내에 적용하여 모체이행항체에 의한 후대 병아리의 우수한 방어 효능을 확인하였다.

Correlation Between Primary Si and Silicide Refinement Induced by Ultrasonic Treatment of Multicomponent Al–Si Alloy Containing Ti, Zr, V, and P

Jae‑Gil Jung,Young‑Hee Cho,Tae‑Young Ahn,Jae‑Hee Yoon,Sang‑Hwa Lee,Jung‑Moo Lee 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.5

The correlation between primary Si and silicide refinement induced by ultrasonic treatment (UST) of multicomponent Al–Sialloy containing Ti, Zr, V, and P was investigated. UST significantly refined the primary Si phase owing to cavitation-inducedwetting and deagglomeration of MgAl2O4particles. Lowering the UST finish temperature caused deterioration of the degreeof primary Si refinement, instead leading to silicide refinement. Cavitation-induced silicide nucleation on wetted MgAl2O4consumed the MgAl2O4particles, particularly in the case of primary Si nucleation. Similarly, the formation of an AlP phaseon the silicide phase reduced the nucleation efficiency of the AlP phase. Poisoning of the MgAl2O4and AlP phases by thesilicide phase was responsible for the deterioration in primary Si refinement. Room-temperature tensile strength and hightemperatureelongation were increased by UST and were dependent on the size of primary Si.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

β-Carotene과 알코올을 섭취한 쥐의 혈액 성분변화의 상관관계

고재웅,조만희,이상한,우기민,염윤기,김창세 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The study was carried out to investigate the effects of dietary carotenids and β-carotene on the hematological changes caused by alcohol administration into the rats. The results were analyzed using several statistical methods; SPSS computer program for measuring the mean values and standard deviations, one way ANOVA for the test of significance, Duncan method for post HOC multiple comparison, and Pearson method for measuring the correlation coefficient. The following are the conclusion from those statistical analysis. 1. ALB and TB were shown to have the most significant correlation among the blood compositions f개m the basic dietary groups. 2. BUN and CHO were shown to have the most positive correlation among those from the carrot-fed groups. 3. CA and AG were shown positively correlated, and CHO and IP negatively correlated, respectively among those from the cheese-fed groups. 4. AP was shown positively correlated among those from the alcohol-administrated groups. 5. CHO, CA and BUN were shown positively correlated, and CA was negatively correlated among those from the β-Carotene -fed groups. 6. TB and TP were not affected by the correlations between the blood composition from all the experimental groups.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

보행환경이 보행에 미치는 영향

조강희,유재욱,김봉옥,윤승호 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

Fall-related accidents account for more than 800,000 injuries and 13,000 fatalities a year in North Americain. And also it is estimated that one-third of all persons 60 years old and older suffer falls each year. The Balance and posture are affected by vision, vestibular system and somatosensory system(proprioception). Three influences related to the older population's risk of falling are normal aging progress, pathology that increases with age and environmental conditions. In this study, to understand the effect of various environmental conditions on kinetic characteristics of initiation of gait and standing posture, vertical ground reaction forces(VGRF), center of pressurc(COP), postural analysis and psychologic index were evaluated in 20 men with no history of neuromusculoskeletal abnormality. The results are 1) VGRF according to various environmental conditions are not significant change. 2) Temporal analysis of IOG according to carpet and wood floor are significantly slowed in wooden floor. 3) Temporal analysis of IOG with or without shoes are not significnat change. 4) The effects of illumination to balance increased maximal oscillation by decreased of illumination

레니텍^(�) 정(말레인산 에날라프릴, 10mg)에 대한 에나레이스 정의 생물학적 동등성

조성희,하용화,홍성제,서성훈,류재환,김동현,이경태 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The purpose of present study was to evaluate the bioequivalence of two enalapril maleate tablets, Renitec^(™) (MSD Korea Ltd.) and Enalace ^(™) (Welfide Korea Ltd.), according to the guidelines of Korea Food Drug Administration (KFDA). Twenty-four normal male volunteers, 22.33±2.55 year in age and 66.54±8.30 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After two tablets containint 10 ㎎ of enal-april maleate per tablet wre orally administered, blood was taken at predetermined time intervals and concentrations of enalapril in plasma were determined using LC-MS-MS. Pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t) and C_(max) untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transfored data were acceptance range of log0.8 to log1.25(e.g., log1.02∼log1.14 and log1.03∼log1.19 for AUC_(t) and C_(max), respectively). The major parameters, AUC_(t) and C_(max), met the criteria of KDFA for bioequivalence indicating that Enalace^(™) tablet is bioequivalent to Renitec^(™) tablet.

용융염산화 반응기에서 기체체류량 및 기-액 흐름특성

조용준,양희철,은희철,유재형,김준형 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.5

용융염산화는 혼성폐기물과 유해폐기물을 효과적으로 산화 및 분해할 수 있는 소각대체기술중 하나이다. 본 연구에서는 직경이 0.079 m이고 높이가 0.653 m인 용융염산화 반응기에서 기체체류량 및 기-액 흐름특성에 대한 연구를 수행하였다. 액상으로 용융탄산나트륨을 기상으로 건조된 공기를 사용하였으며 기체유속(0.05-0.22 m/s)과 용융염온도(870-970℃) 변화가 기체체류량 및 기-액 흐름특성에 미치는 영향을 규명하였다. 기체체류량은 용융염 온도가 상승함에 따라 증가하였는데, 이는 용융염 온도의 증가로 인해 용융된 탄산나트륨의 점도와 표면장력이 감소하였기 때문이다. 실험에서 얻어진 기체체류량 데이터를 drift-flux 모델에 적용하여 용융염반응기 내의 흐름특성을 규명할 수 있었으며, 이를 통해 흐름영역에 따른 기체체류량을 정확하게 예측할 수 있었다. Molten salt oxidation is one of the most promising alternatives to incineration that can be used to efficiently destroy the organic components of mixed wastes and hazardous wastes. In this study, the gas holdup and gas-liquid flow characteristics are investigated in the molten salt oxidation reactor (0.076 m D×0.653 m H.). Effects of input air velocity (0.05-0.22m/s ) and molten salt temperature (870-970℃) on the gas holdup and flow characteristics have been studied. Molten carbonate as the liquid phase and air as the gas phase have been used in this study. The gas holdup increases with increasing molten salt temperature due to the decrease of viscosity and surface tension of molten carbonate. The experimentally obtained gas holdups in the molten salt reactor have been well described and characterized by means of drift-flux model. The gas holdups with variation of the flow regime have been well predicted.

구강에 발생한 편평상피암종의 MMP-2, MMP-9, TIMP-1, TIMP-2 발현과 EGFR 및 E-cadherin 발현의 상호연관성

조성재,권창석,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is related to infiltrative growth and metastasis of tumor. Positive correlation between MMPs expression and clinico-pathologic parameters were reported, and MMPs expressions were regulated by tissue inhibitor of metalloproteinases(TIMPs) or cell adhesion molecules such as E-cadherin and epidermal growth factor receptor EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors such as clinical stage. recurrence. histologic grade and invasion pattern, and between MMP-2 and MMP-9 Expressions and TIMP-1, TIMP-2, and E-cadherin expressions. Methods and Material : The materials were 55 cases of squamous cell carcinoma of the oral cavity, and immunohisdtochemical studies for MMP-2, MMP-9, TIMP-1, TIMP-2, E-cadherin and EGFR were performed. Clinico-pathologic factors such as clinical stage, recurrence, histologic grade and invasion pattern were recorded through microscopic examination and clinical record review. Statistical analyses between MMP-2, MMP-9 expressions and clinico-pathologic factors, TIMP-1, TIMP-2 expressions and EGFR and E-cadherin expressions were done. Results : 1. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, respectively. TIMP-1 and TIMP-2 expressions were observed in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions were found in 35(63.6%) and 26(47.3%) of 55 cases, respectively. 2. MMP-2 expression rate was slightly higher in cases without recurrence, and TIMP-2 expression rate was slightly higher in cases showing more infiltrative growth pattern. However, there were no significant differences of MMP-2, MMP-9, TIMP-1, and TIMP-2 expression rates according to other clinico-pathologic parameters were noted. 3. The expression rate of EGFR was higher in cases with well differentiation (p=0.0047), but no positive relationship between the expression rate of E-cadherin and histologic grade was found. There were no significant differences of the expression rates of E-cadherin and EGFR according to other clinico-pathologic parameters. 4. Cases with positive reaction for MMP-9 showed an increasing tendency of negative reaction for TIMP-1. However, signigicant relationships between MMP-2, MMP-9 expressions and TIMP-1, TIMP-2 expressions were not noted. 5. The expression rate of MMP-2 was higher in cases with positive reaction for Ecadherin and EGFR with no statistical significance. The expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.0221), but there was no significant relationship between MMP-9 and EGFR expression. Conclusion : These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamos cell carcinomas, but MMP-2, MMP-9, TIMP-1, and TIMP-2 expressions might not seem to be a sueful prognostic factors because there were no significant relationship between clinicopathologic parameters, EGFR expression showed positive correlation with low histologic grace, so EGFR expression could be regarded as a good prognostic factor. In the progression of squamous cell carcinoma of oral cavity, MMP-9 and E-cadherin might be involved with positive correlation, but there were no significant correlations between MMP-2, MMP-9 and TIMP-1, TIMP-2 and EGFR expressions rate. Further study for other factors such as cytokines or growth factors except TIMP-1, TIMP-2 and EGFR will be needed.

Phase-Shift를 이용한 시분할형 부하병렬 고주파 인버터의 특성해석에 관한 연구

조규판,원재선,김동희,노채균,원규식,심광열 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.2

A half bridge time sharing type load parallel high frequency resonant inverter to give VVVF function in the inverter used as power source of induction heating at high frequency is presented in this paper. This proposed inverter can reduce distribution of the switching current because of using the current of serial resonant circuit to the input current of the parallel one and this paper also realize the output control of independence irrespective of the switching frequency using Phase-Shift. The analysis of the proposed circuit is generally described by using the normalized parameters. Also, the principle of basic operation and the its characteristics are estimated by the parameters, such as switching frequency, the variation of phase angle(φ) of Phase-Shift. It is certain that the proposed circuit will be used and expanded in the high frequency power supplies like induction heating systems.