수종의 피부종양에서 Proliferating Cell Nuclear Antigen과 Ki - 67 의 표현에 관한 비교 연구

황선욱(Sun Wook Hwang),원영호(Young Ho Won),전인기(Inn Ki Chun),박창수(Chang Soo Park) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.3

Background : Both PCNA and Ki-67 have been used as marker for cellular proliferation. The drawback of Ki-67 antibody in immunohistochemistry was that it can be labelled only on fresh tissue, however, MIB1 is a newly developed Ki-67 antiboc which can be labelled on formalin-fixed tissue. Objective : The purpase of the present study is to compir the stainability of the Ki-67 antibody with that of the ICNA antibody on formalin-fixed, para fin embedded tissues. Methods : Using MIE1, the newly developed Ki-67 antibody and PC10(PCNA antibody), speci mens of squamous cell carcinoma(SCC), Bowens disease(BL), actinic keratosis(AK) and basal cell epithelioma(BCE) were stained by one hour immunocytial, mistry using a Microprobe immuno/DNA stainer. Results : The labelling indices (LI) of MIB1 were 82.6%, 37.4%, 38.3% & 81.1% respectively in SCC, BD, AK & BC, while the LI of PC10 were 77.69%, 26.6% & 64.4%. The labelled cells of both antibodies differed in distribution patterns on turmor tissues. Conclusion : MIBI cain be used to be an alternative m.rl r for proliferating cells. MIBI PC10, when used together, will be mutually compensatory the study of proliferating cell kinetics. (Kor J Dermatol 1995;33(3): 453-458)

Systematic Review of Reciprocal Changes after Spinal Reconstruction Surgery : Do Not Miss the Forest for the Trees

Kim, Chang-Wook,Hyun, Seung-Jae,Kim, Ki-Jeong The Korean Neurosurgical Society 2021 Journal of Korean neurosurgical society Vol.64 No.6

The purpose of this review was to synthesize the research on global spinal alignment and reciprocal changes following cervical or thoracolumbar reconstruction surgery. We carried out a search of PubMed, EMBASE, and Cochrane Library for studies through May 2020, and ultimately included 11 articles. The optimal goal of a truly balanced spine is to maintain the head over the femoral heads. When spinal imbalance occurs, the human body reacts through various compensatory mechanisms to maintain the head over the pelvis and to retain a horizontal gaze. Historically, deformity correction has focused on correcting scoliosis and preventing scoliotic curve progression. Following substantial correction of a spinal deformity, reciprocal changes take place in the flexible segments proximal and distal to the area of correction. Restoration of lumbar lordosis following surgery to correct a thoracolumbar deformity induces reciprocal changes in T1 slope, cervical lordosis, pelvic shift, and lower extremity parameters. Patients with cervical kyphosis exhibit different patterns of reciprocal changes depending on whether they have head-balanced or trunk-balanced kyphosis. These reciprocal changes should be considered to in order to prevent secondary spine disorders. We emphasize the importance of evaluating the global spinal alignment to assess postoperative changes.

급성 심근허혈에 따른 심실활성시간(Ventricular Activation Time)의 변화

한성욱,김윤년,김기영,남창욱,김기식,김권배 대한순환기학회 2001 Korean Circulation Journal Vol.31 No.3

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Acral calcified angioleiomyoma: a rare clinicopathologic variant of cutaneous angioleiomyoma

( Kihyuk Shin ),( Woo-il Kim ),( Min-young Yang ),( Won-ku Lee ),( Tae-wook Kim ),( Sung-min Park ),( Hyun-joo Lee ),( Hoon-soo Kim ),( Hyun-chang Ko ),( Byung-soo Kim ),( Moon-bum Kim ),( Gun-wook Ki 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Cutaneous angioleiomyomas are benign tumors with vascular smooth muscle differentiation. The tumor usually involves the lower extremity, but acral sites such as toes, fingers, feet, and hands are rarely affected. Leiomyoma of deep soft tissue commonly shows regressive changes including prominent fibrosis, and calcification, however, cutaneous angioleiomyoma rarely manifests these changes. A 61-year-old female presented with subcutaneous nodule on the right heel. The lesion began to develop 3 months ago without history of trauma or infection, and the patient reported no subjective symptom. The lesion was totally excised, and histopathology revealed a well-circumscribed, non-encapsulated tumor composed of interlacing fascicles of spindled cells with elongated blunt-ended nuclei. A major finding was extensive calcifications, which dominated over the cellular component. On immunohistochemical examination, the spindle cells expressed strong immunoreactivity for α -smooth muscle actin. Based on these findings, a diagnosis of acral calcified angioleiomyoma was made. We have described a rare clinicopathologic variant of angioleiomyoma which has a predilection for acral locations and shows extensive calcifications predominating over the tumor itself. To our knowledge, this is the first report of a patient with acral calcified angioleiomyoma in Korean dermatologic literature.

상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

1,3-Disubstituted Pyrido [2,3-d] Pyrimidinedione

류정욱,이기창,강삼룡 明知大學校 産業技術硏究所 1984 産業技術硏究所論文集 Vol.3 No.-

1,3-Disubstituted pyrido [2, 3-d] pyrimidine-2, 4-diones derivatives were synthesized by the following method A and B. Method A: 1-Substituted pyrido [2, 3,-d] pyrimidine-2, 4-diones were prepared by the reaction of 2-[N-substituted amino] nicotinic acid with urea at high temperature. On the other hand method B:1-Substituted pyrido [2, 3-d] pyrimidine-2 4-dione prepared by the reaction of 2-[N-substituted amino] nicotinamide with ethyl carbonate at low temperature. It was largely affected by catalyst and temperature that the synthesis of 2-[N-substituted amino] nicotinic acid as starting material, and amide of 2-[N-substituted amino] nicotinic and derivative was synthesized by the typical nucleophilic substitution of acyl halide. Considering the reaction time and temperature, however, it is clear that the method B is more efficient than the method A. The former requires only 2-3 hours of reaction time at 20-40℃, while the latter requires 7 hours at 180-200℃. Yields for two methods obtained were 60% all. Yields of 1, 3-disubstituted pyrido [2, 3-d] pyrimidine-2, 4-dione as object was obtained about 60% by the reaction of intermediate material with alkylated in the DMF solvent. It has been affirmed that the mechanism of reactions involved in synthesis was of nucleophilic substitution. The molecular structure, and molecular weight of 1, 3-disubstituted pyrido [2, 3-d] pyrimidine-2, 4-diones were confirmed by the analysis of physicochemical instrumentation such as IR, NMR, and Ms spectra, and the physical properties were examined.

동종 조혈모세포이식 후 폐합병증 : 미만성 폐침윤에서 기관지경의 유용성

민창기,엄기성,이동건,김동욱,이종욱,민우성,김춘추,정정임,김태연,송정섭 대한조혈모세포이식학회 2000 대한조혈모세포이식학회지 Vol.5 No.1

배경:동종 조혈모세포이식을 받고 백혈구수가 회복한 이후의 폐합병증은 대부분 국소적 폐렴이 아닌 미만성의 폐침윤(diffuse pulmonary infiltrate) 소견을 보이고 이식 후 불완전한 면역 재구성 또는 이식편대 숙주질환(graft-versus-host disease; GVHD) 등과 관련된다고 알려져 있다. 원인은 세균보다는 바이러스(주로 cytomegalovirus; CMV), Pneumocytis carinii (PC) 등이며 원인을 규명할 수 없는 특발성의 경우도 상당수를 차지하고 있으나 아직 국내에서는 자세히 보고된바 없다. 저자들은 본 센터에서 동종 조혈모세포이식 후 발생한 폐합병증의 진단 및 치료성적을 보고하고자한다. 방법:1997년 1월부터 1999년 8월까지 본 센터에서 동종 조혈모세포이식을 받은 321예의 환자 중에 방사선학적으로 미만성 폐침윤을 보여 기관지경 검사를 받은 환자 35예를 대상으로 하였으며 5예의 환자는 임상적인 경과의 변화로 기관지경 검사를 2회 실시 받았다. 환자들의 임상적 특성, 영상학적 검사 그리고 기관지경 검사로 기관지 폐포세척술을 실시하여 얻은 가검물에서 원인 미생물을 배양하거나 폐조직 생검을 통하여 진단하였다. 각 환자에서 진단에 따른 치료 및 반응을 관찰하였다. 결과:1) 환자연령의 중앙치는 31 (16~40)세, 이식 후 미만성 폐침윤이 발생하기까지 기간의 중앙치는 162 (55~965)일 이었다. 이식 당시 질환은 CML 16예, AML 10예, ALL 5예, NHL 2예, MDS 1예 그리고 CLL 1예 이었으며 35예 중 11예에서 2차 관해 이상의 진행된 질환을 갖고 있었다. 35예 중 비혈연간 또는 혈연간 HLA 부분일치 동종 조혈모세포이식 환자는 12예였고, 전처치에 전신 방사선조사를 받은 환자가 27예 항암제만 받은 환자는 8예 이었다. 2) GVHD 예방 약제로는 cyclosporin+methotrexate (n=26) 또는 tacrolimus+methotrexate (n=9) 조합을 사용하였다. 감염 예방으로 경구로 trimethoprim-sulfamethoxazole, acyclovir을 투여를 받은 환자는 9예(25.7%) 이었다. II도 이상의 급성 GVHD를 가진 환자가 12예(34.3%) 이었고, 27예(77.1%)에서 만성 GVHD를 갖고 있었다. 3) 기관지 폐포세척술의 가검물 배양 결과 미만성 폐침윤의 원인 미생물로는 CMV 8예, PC 6예, 두 미생물의 혼합감염 10예, 폐렴구균 1예, 그리고 Mycobacterium tuberculosis 1예 이었고, 나머지 14예(35%)에서는 원인균이 동정되지 않았다. 경기관지 폐조직생검은 19예 중 3예(15.8%)에서 진단적 가치가 있었다. 4) 기관지경을 시행한 40예 중 8예(20%)에서 폐포세척술 검체의 배양 및 경기관지 폐생검의 결과에 따라 치료를 하였음에도 불구하고 호전되지 않았다. 5) 35예 중 9예의 환자가 사망하였으며 이중 5예는 폐합병증으로 사망하였고 나머지 4예는 재발 또는 이식편대숙주질환에 의해 사망하였다. 폐합병증으로 사망한 환자의 원인으로는 CMV 1예, PC 1예, 혼합감염 1예, 그리고 원인 불명의 폐합병증이 2예 있었다. 결론:동종 조혈모세포이식 후 발생한 미만성의 폐침윤의 주된 원인은 CMV, PC 등이며 단독 감염보다는 혼합 감염의 형태를 보였다. 기관지경 검사 및 폐포세척술은 미만성 폐침윤의 진단에 유용하며 안전한 시술로 사료된다. 폐합병증은 주로 만성 GVHD 환자에서 출현하였으며 원인이 밝혀지지 않은 경우에 면역억제 치료가 효과적이었다. Background:Diffuse pulmonary infiltrates (DPI) after a neutropenic phase following allogeneic stem cell transplantation (allo-SCT) are common and include various causes. Multiple factors are thought to contribute to these pulmonary complications, including the graft-versus-host disease (GVHD), incomplete immune reconstruction and infections. This study was undertaken to determine the results of fiberoptic bronchoscopy (FOB) in patients with DPI after allo-SCT. Methods:Three hundreds twenty one patients underwent allo-SCT from January 1997 through August 1999 at Catholic Hemopoietic Stem Cell Transplantation Center. Out of 321 patients, 40 FOBs were performed in 35 patients who were aged 16 to 40 years (median age 31 years). In all cases, bronchoalveolar lavage (BAL) was achieved. Transbronchial forceps biopsy (TBFB) was also carried out in 47.5% (19/40). Clinical, radiologic, pulmonary function, microbiological and pathologic tests were reviewed. Results: 1)The underlying diseases were predominantly chronic myeloid leukemia and acute leukemia (31/35, 88.5%). Median time to FOB following SCT was 162 days (range, 55-965). The patients with acute GVHD more than grade II were 34.3% (12/35) and those with chronic GVHD were 77.1% (27/35). 3) An etiological diagnosis could be obtained in 26/40 (65%) of cases; mixed infection of cytomegalovirus (CMV) and Pneumocystis carinii (PC) was the most common finding (10/40, 25%), CMV and PC were isolated in BAL in 8/40 and 6/40 respectively, Pneumococci in one and Mycobacterium tuberculosis in one. In 14 procedures (35%), the results were negative. TBFB were valuable for diagnosis for only 3 out of 19 patients. There was one minor bleeding following FOB and TBFB. 4) Thirty two cases (80%) of FOB improved after treatment according to etiology. The patients with unknown etiology showed a good response to steroid (11/14, 78.6%). 5) Overall mortality in patients with DPI was 9/35 (25.7%). Five episodes of DPI were to refractory to treatment and were causes of death. Five causes consisted of 1 CMV, 1 PC, 1 mixed infection, and 2 unknown etiologies. Conclusion:FOB is a safe and valuable procedure for the evaluation of DPI after allo-SCT, which were commonly associated with GVHD, CMV and PC. The mixed infections were more predominant than those due to single isolated organism. Steroid was effective for patients with DPI in whom causative organisms were not able to be identified.

충남대학교 공업기술 계열 교사 연수 프로그램에 대한 만족도 분석

류창열,최유현,김판욱,김기수,김태훈,이창훈 忠南大學校 工業敎育硏究所 2005 論文集 Vol.27 No.1

The purpose of this study was to analyze the educated teachers' satisfaction of the in-service education programs of industrial technical and technology teachers in Chungnam National University. This study was carried out through survey to accomplish the purpose. The number of questionnaires collected was 86. This study used the SPSS program to conduct a descriptive analysis of the data. The results of this study were as follows: (1) The satisfaction about 'teachers' participation', 'utilization of audiovisual materials and instruments' and 'level of professor and lecture' was relatively high. (2) The satisfaction about 'environments of in-service education programs', 'evaluation of achievement', and 'efficacy of lectures' was relatively low.

FET형 반도체 바이오센서를 위한 웨이퍼 규모의 효소 고정화막 형성기술

김창수,조병옥,최성문,남동현,김의락,손병기 경북대학교 센서기술연구소 1991 센서技術學術大會論文集 Vol.2 No.1

To improve the standardization of enzyme membranes for FET-type biosensor, wafer-scale formation technique had been studied by using photo-sensitive polymers. Glucose Oxidase, the enzyme which decomposes the glucose, had been used for application of this method to glucose sensors. Thin standardized memebranes could be obtained by spin coating of the mixed solution, composed of PVA-SbQ polymer and glucose oxidase, followed by photolithographic techniques. The performance characteristics of the glucose sensor having this thin enzyme membrane had been investigated.