수종의 피부종양에서 Proliferating Cell Nuclear Antigen과 Ki - 67 의 표현에 관한 비교 연구

황선욱(Sun Wook Hwang),원영호(Young Ho Won),전인기(Inn Ki Chun),박창수(Chang Soo Park) 대한피부과학회 1995 大韓皮膚科學會誌 Vol.33 No.3

Background : Both PCNA and Ki-67 have been used as marker for cellular proliferation. The drawback of Ki-67 antibody in immunohistochemistry was that it can be labelled only on fresh tissue, however, MIB1 is a newly developed Ki-67 antiboc which can be labelled on formalin-fixed tissue. Objective : The purpase of the present study is to compir the stainability of the Ki-67 antibody with that of the ICNA antibody on formalin-fixed, para fin embedded tissues. Methods : Using MIE1, the newly developed Ki-67 antibody and PC10(PCNA antibody), speci mens of squamous cell carcinoma(SCC), Bowens disease(BL), actinic keratosis(AK) and basal cell epithelioma(BCE) were stained by one hour immunocytial, mistry using a Microprobe immuno/DNA stainer. Results : The labelling indices (LI) of MIB1 were 82.6%, 37.4%, 38.3% & 81.1% respectively in SCC, BD, AK & BC, while the LI of PC10 were 77.69%, 26.6% & 64.4%. The labelled cells of both antibodies differed in distribution patterns on turmor tissues. Conclusion : MIBI cain be used to be an alternative m.rl r for proliferating cells. MIBI PC10, when used together, will be mutually compensatory the study of proliferating cell kinetics. (Kor J Dermatol 1995;33(3): 453-458)

색소실조증의 임상적 연구

전인기(Inn Ki Chun),정태복(Tae Bock Chung),김영표(Young Pio Kim) 대한피부과학회 1985 大韓皮膚科學會誌 Vol.23 No.2

The clinical observation was done on 9 patients of incontinentia pigmenti(I,P.) who were seen at the Department of Dermatology, Chonnam University Hospital from January, 1970 through December, 1982. The incidence of I.P. patients among the population of Chonnam province was higher than 1/142,000 whereas the frequency of I.P. among the outpatients was 0.016% The sex of I.P. patients was all female and there was no cases showing faiailial occurrence. The onset of the cases were within 4 months after birth, 44%, being at birth and 89% being within 2 weeks after birth. The durations of first, second and third stages of I.P. patients were respectively 14 days, 15 days to 2 months, and 2 months to 10 months. The trunk and extremities were involved in 4 cases, and the extremities only were involved in 4 cases while the trunk only was involved in J case. The blood eosinophilia was observed in 2 cases among 7 cases, with their results being 18%, and 11% respectively. The biopsy examinations done in 7 cases at the time of their first visits revealed that 3 cases, 2 cases and 1 case respectively conformed to first, second and third stages with 1 case having first and second stages simu 1taneously. There was no I.P. patient showing the associated congenital abnorma- lity.

피부묘기증 (皮膚描記症) 환자의 정신상태에 관한 연구

전인기(Inn Ki Chun),박미순(Mi Soon Park),김승훈(Soung Hun Kim),김영표(Young Pio Kim) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.3

The subjects of the present study was 300 out-patients and 304 people living in similar condition. The SCL-90(symptom check list-90) questionare was used and the two groups were compared by many factors. The rcsults were as follows. 1. All dimensional values of the dermographism group were much higher than the normal group except obsessive-compulsive dimension. The order of significance: Paranoid ideation, interpersonal sensitivity somatization, depression, anxiety, hostility and phobic anxiety(p<0. 01), The result indicates a significantly high incidence of psychological problems among dermographism patients. 2. In the dermographism group, the patients over 30 years old had significantly higher. scores in depression, anxiety, hostility and psychoticism. 3. Male patents showed significantly higher scores than female in paranoid ideation. 4. The unmarried patients had significantly higher scores in dcpression, para noid ideation and psychoticism. 5. Thc longer the duration of the disease was the higher the scores were. 6, There were no significant differences in educational status, family history, the job at onset of the disease, and the accuracy of understanding about the disease.

나환자 임파절내 T - 임파구의 조직화학적 관찰 및 분석에 관한 연구

전인기(Inn Ki Chun),유영은(Young Eun Yoo),김영표(Young Pio Kim) 대한피부과학회 1983 大韓皮膚科學會誌 Vol.21 No.4

Aplasia cutis congenita is a rare disease presenting in the newborn infant as localized areas of skin defect. Its association with a number of congenital malformations is well documented. I observed a newborn infant with typical aplasia cutis congenita. The skin defect of my case was found on the posterior fontanelle without any congenital malformation. Diagnosis was made by history, clinical and histopathological findings.

간질환 환자의 피부소견에 대한 임상적 관찰

전인기(Inn Ki Chun),박경재(Kyung Jae Park),김영표(Young Pio Kim) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.6

From September 1983 to August 1984, the clinical observations regarding cutaneous manifestations of 26(j cases of in-patients in the Department of Internal medicine with hepatic problems were observed at the Department of Derrnatology in Chonnam University Eiospital, The results were as follows: In the 266 patients, 201 patients(75.6%) revealed various cutaneous manifestaiions; Among them vascular spider occured in 100 (37. 6%), bleeding tendency in 67(25. 2%), pruritus in 65(24.4%), jaundice in 59 (22.2%), palmar erythema in 59(22. 2%), and melanosis in 56(21. l%). The incidences of skin changes by pattern of liver disease were as follows; In acte hepatits, prurit;us(46.8%) and acute urticarial & maculapapular eruption(36. 4%) were found in order. In chronic hepatitis, vascular spider(42. 6%) and melanosis (27. 9%) in order. In cirrhosis, vascular spider (62. 5%) and bleeding tendency (42. 0%) in order. In hepatic cancer vascular spider (24. 2%) and jaundice (21. 2%) in order. There were no statistically significant differences in sex distribution. In the incidences of skin changes by age groups, jaundice(p<0. 01) and melanosis were more frequently observed in the patients under the age of 29 and vascular spider (p<0.01 ) and melanosis in the patients are the age of 50. The longer the duration of illness were, the higher the incidences of skin changes except jaundice were.

대상포진과 전신질환과의 관계에 관한 연구

전인기(Inn Ki Chun),이재조(Jai Jo Lee),원영호(Young Ho Won),김영표(Young Pio Kim) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.3

This study was designed to observe more closely about clinical course, complication, predilection site of skin eruption and associated diseases with herpes zoster. Total 122 cases of herpes zoster were prospectively observed from April 1986 to July 1987. The results obtained were as follows : 1. The ineidence of herpes zoster versus the total number of outpatients was 20.6%. There was no difference in sex ratio of male(52.5%) to female(47.5%). 2. Herpes zoster was observed most frequently in the 50-59 year age group (25.4%). 3. There were no marked monthly or seasonal difference in herpes zoster without an explosive outbreak or an epidemic cycle. 4, We found that 68 cases(55.7%) were associated with underlying diseases : the most common disease was hypertension(23 cases), the next one was malignsncy(10 cases), namely, lung cancer, Hodgkin's lymphoma, other malignant lymphoma, leukemia and mediastinal mass, and the other diseases were tuberculosis of intenal organs, diabetes mellitus, hepatic diseases, cardiac diseases, chronic pulmonary diseases, cord injury, gastric ulcer, brain damage, burn, renal diseases, rheumatoid arthritis, and measles. 5. It was suggested that the distributuon of skin lesion were related to dermatome. The incidence of skin lesion was distributuon in the following region in order : T5-T8(25.4%), C6-T4(24.6%), Cl-C5(20.5%), Ll-S3(13.9%), Vl-V3 (12.3%), and T9-T12(3.3%), there was involvement of the entire thoracic dermatome(C6-T12) in 65 cases(53.3%). A great many cases of malignancy, chronic pulmonary disease, cardiac disease, tuberculosis and gastric ulcer were most frequently distributed in the thoracie dermatome, but the involvement of ophthalmic dermatome was found only in the patients with hypertension. 6. The most common complication was postherpetic neuralgia for 31 cases(25.4%) with the highest frequency in 60's(32.3%). Other complications were ophthalmic complication, Ramsay-Hunt syndrome, secondary bacterial infection, moter nerve involvement, difficulty of urination, and recurrent herpes zoster.

방사선 치료후 전이를 보인 기저세포암

전정인 ( Chung Inn Chun ),이승철 ( Seung Chul Lee ),원영호 ( Young Ho Won ),전인기 ( Inn Ki Chun ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

Basal cell epithelioma is a common neoplasm that rarely metastasizes. The majority of metastatic BCE originates from chronic large, ulcerated tumors which have been refractory to several modalities of therapy. It usually metastasizes to regional lymph nodes, lung, bone, skin and liuer but any other organs may b~e also involved. We report a case of metastatic basal cell carcinoma in a 73-year-old male who had a large ulcerated tumor mass on the left orbital and periorbital area for 13 years. He received radiation therapy 4 years ago, which seemed to cause exaggeration of the ulcerative mass ensuing left side eyeball destruction. About 1 year ago, he first noticed an 1 x 1cm sized hard palpable mass on the left side of neck. Cervicil CT shows 1 * 1cm sized central necrotic tumor mass on the left cervical lymph node. Biopsy specimens taken from the left periorbital area and the left cervical mass revealed typical histopathologic findings of the basal cell carcinoma. The diagnosis of metastatic basal cell carcinoma wa,, finally made on clinical, radiological and histopathological findings. The occurence of MBCC has been reported rarely in Korean literature, so we report, this case of the MBCC of cervica~l lymph node after radiation therapy. (Kor J Derrnatol 1996;34(5): 810-814)

자외선 조사에 의한 일광화상 세포 형성에 미치는 국소 도포제의 영향에 관한 연구

전정인 ( Chung Inn Chun ),이승철 ( Seung Chul Lee ),전인기 ( Inn Ki Chun ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.3

Background: The sunburn cell is an abnormal keratinocyte of the skin induced by ultraviolet (UV) irradiation, and is used as an indicator of cell damage. The sunburn cell is consideredas an apoptotic cell which has been caused by UV irradiation, and many studies have been performed to understand the mechanism of photodamage in relation to apoptosis. The mechanism of photodamage by UV irradiation is still unclear. However, it is suggested that oxygen stress by reactive oxygen species produced by the UV rays may play an important role. Objective : This study was a med at evaluating whether various antioxidants and sunscreen can prevent sunburn cell formation. In addition, we studied whether or not the sunburn cell is identical to an apoptotic cell stained using the TUNEL method. Methods : White mice (ICR strain) were used to test the potency of various topical agents which are used in the prevention of sunburn cell formation; the agents were various antioxidants of L-ascorbic acid, tocopherol, catalase, a reduced form of glutathione (GSH), and sunscreen PABA (para-amino benzoic acid). Each agent was topically applied daily for 5 consecutive days on the dorsal skin of the ears. 300 mJ/cm of UV-B was irradiated on the ears 30 mins after the final applicaion, and skin samples were taken 24 hrs after that. The sunburn cells in the H & E stain were counted per 1 mm under the microscope. Also, the same sections for the sunburn cell study were stained by the TUNEL method using the ApopTag In Situ Apoptosis Detection Kit (Oncor, Inc.). Results : The number of sunburn cells increased in a UV-B dosage-dependent. manner up to 300 mJ/cm2. The potency in the reduction of sunburn cell formation was as follows in order,PABA (0.37 + 0,5), GSH (0.87 + 0.5), ascorbic acid (1.62 + 0.85) and tocopherol (1.75 + 1.12). However catalase (2.93 + 1.56) did not show any protective effect. Also, the finding that sunburn cells were the same as TUNEL-positive cells confirmed the notion that the sunburn cell is a kind of apototic cell. Conclusion : A sunburn cell is a kind of apoptotic cell that may be caused by reactive oxygen species induced by UV-B irradiation, in view of the fact that sunburn cell formation was inhibited by the topical application of various antioxidants. But the result that physical protection by PABA has the most potent protective effect in relation to sun damage suggests that protection using a combined physical and biochemical approach is important in the development of new topical agents which will inhibit sundamage to the skin. (Kor J Dermatol 1996;34(3): 394-401)

농미충증 (농미충증) 의 임상 및 역학적 고찰

김영표,전인기,하범석 ( Young Pio Kim,Inn Ki Chun,Bom Seock Ha ) 대한피부과학회 1989 大韓皮膚科學會誌 Vol.27 No.5

Taenia aolium is responsible for producing human intestinal infection with the tapeworm (taeniasis) and the lodging of the larval stage (Cysticercua cellulosae) in numerous organs. especially subcutaneous tissue with the prodution

고정형에서 임파형으로 속발된 스포로트리쿰증

박미순,전인기,김영표 ( Mie Soon Park,Inn Ki Chun,Young Pio Kim ) 대한피부과학회 1988 대한피부과학회지 Vol.26 No.3

We experienced 53-year-old male patient, who had an eczematous verrucous plaque measuring 6 x 4cm in diameter on the nasal bridge for 4 months. On physical examination, there was no abnormality except of palpable bean sized nodule on the left side of chin for 2 months. The overlying skin of the nodule is a quite normal appearance. The skin biopsy of the nodule showed the charact.eristic a.rrangement of the infiltrate in three zones. And the result of fungus culture of the thin tissue slice on Sabouraud media diagnosed fungal infection due to Sporotherix .schenkii. The treatment began with oral ketoconazole for 4 weeks, and the medication was interrupted for 2 weeks, because of no favorable effectiveness. On his revisit, a chain of painless nodules along lymphatics palpated on the right side of his f ace. We changed the therapy into saturated solution of potassium iodide(KI) with 0.25rnl(5 drops) orally there times a day and was gradually increased administration of KI, And no side effects were noted. The patient was treated for a. total of 18 weeks, which included 6 weeks of treatment following clinical resolution.