서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

Claudin-1 expression decreases with increasing pathologic grade in actinic keratosis and may be used as a marker of high-risk actinic keratosis

( Ji Su Lee ),( Hyun-sun Park ),( Hyun-sun Yoon ),( Soyun Cho ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Actinic keratosis (AK) is a common sun-induced skin disorder of importance for the progression to invasive squamous cell carcinoma. However, there is still no good way to predict high risk AK. Objectives: To identify markers which reflect the biologic behavior of AK and to understand the pathogenesis of AK Methods: A total of 52 patients with AK and site-matched 17 normal controls were included. We evaluated solar elastosis and immunohistochemical features using the following antibodies: p53, vitamin D receptor (VDR), claudin-1, and Langerin. Results: Solar elastosis increased and Langerhans cell (LC) density decreased with aging in both patients and controls. Solar elastosis and p53 expression were higher and VDR expression was lower in patients than controls; however, they showed no statistical difference in relation to the pathologic grade of AK. Claudin-1 expression gradually decreased from normal control to severe AK and decreased in the areas with epidermal atypia. LC density in severe AK was significantly lower than in normal control and mild AK; no difference in LC density was seen among control, mild AK and moderate AK. Conclusion: Claudin-1 can be a useful marker of pathologic severity of AK. In contrast, p53 increases and VDR decreases in AK not in gradual manner but in the early steps of carcinogenesis. LC density is relatively maintained in AK until it reaches severe dysplasia.

Hairless Mice를 이용한 광노화 모델에서 APB-01의 경구반복투여에 의한 피부주름개선 효과 시험

이지해(Ji-Hae Lee),이병석(Byung-Suk Lee),변범선(Bum-Sun Byun),김완기(Wan-Gi Kim),이상준(Sang-Jun Lee),심영철(Young-Chul Sim),김배환(Bae-Hwan Kim) 한국독성학회 2003 Toxicological Research Vol.19 No.4

Ultraviolet (UV) is thought to induce erythema, sun-burn, photo-toxicity, photo-allergy, photo-aging and sometimes skin tumor. To investigate the photo-protective effects of APB-01 (Amore-<br/> Pacific Beauty-01, the mixture of Jaummi-dan and Fujiflavone P10) on UV-induced skin damage, forty of SKH hairless female mice were orally administered with APB-01 or saline fifth a week, and irradiated with UV third a week for up to ten weeks. We examined the relationship between visible changes and skin damage in the dermis and epidermis. In the APB-01 treated group, a better skin<br/> and less wrinkles formation were observed when compared to the UV control group. This results demonstrated that oral administration of APB-01 seems to have photo-protective effects on UV-induced<br/> skin damage of hairless mice due to an inhibitory effect on collagen breakdown, and the model using hairless mice is very useful to investigate the efficacy of functional beauty foods.

가축에서 분리된 Vancomycin 내성 장구균의 내성유전자 구조 분석을 통한 분자생물학적 역학 연구

허지영,이선민,이석호,이위교,용동은,이경원,신완식,이동건 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

중추성 요붕증이 동반된 랑게르한스 세포 조직구증 1예

김진호,문준성,문선중,이지은,최재원,은미정,천경아,조인호,윤지성,원규장,이경희 신덕섭,이형우 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Central diabetes insipidus (DI) is a syndrome characterized by thirst, polydipsia and polyuria. Langerhans cell histiocytosis is one of the etiologies of DI. Recently we experienced a central DI associated with Langerhans cell histiocytosis. The 44 years old female patient complained right hip pain polydipsia and polyuria. We carried out water deprivation test. After vasopressin injection, urine osmotic pressure was increased form 109mOsmol/Kg to 327mOsmol/Kg (300%). Brain MRI showed a thickened pituitary stalk and at hot bone CT.CT guided biopsy revealed abnormal histiocytes proliferation and abundant lymphocytes, The final diagnosis was central DI associated with systemic Langerhans cell histiocytosis invading hip bone, L-spine and pituitary stalk. Desmopressin and etoposide chemotherapy were performed to the patient.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

비행청소년을 위한 대집단 사이코드라마의 치료요인

이후경,현지은,윤성철,김선재 대한신경정신의학회 2000 신경정신의학 Vol.39 No.6

연구목적 : 본 연구는 초범 및 경범인 비행청소년을 위한 대집단 사이코드라마(이하 사이코드라마라 함)를 개발하여 실시하고, 치료요인을 중심으로 비행청소년 사이코드라마의 효과 및 특징을 조사하여, 사이코드라마를 비행청소년을 위한 효과적, 효율적인 치료프로그램으로 활용할 수 있는 기반을 마련하고자 하는 목적으로 실시되었다. 방 법 : 연구대상은 1998년 7월부터 12월까지 서울시립청소년수련관의 사랑의 교실에 참여한 비행청소년 827명(남자759명, 여자 68명)이었으며, 나이는 평균 15.92세였다. 비행청소년에 적용되는 사이코드라마 개발을 위해 정신과전문의를 중심으로 한 치료팀을 구성하였고, 기초작업으로는 1998년 1월부터 6월까지 사이코드라마를 시행하면서 ①warming-up(소집단모임), ③action(상황극과 본극), ③sharing(공유)으로 이루어진 기본구조를 만들었으며, 진행의 기본목표와 기본원칙을 설정하였다. 사이코드라마시행한 후 매 회마다 13가지 치료요인 척도로 치료요인을 조사하고, 사이코드라마 평가 설문지로 사이코드라마에 대한 유익도, 흥미도, 참여도를 조사하였다. 결 과 : 본 사이코드라마에 대한 평가 결과를 보면 47.4%가 유익한 것으로 평가하였고 이에 비해 12.1%만이 유익하지 않았다고 하였으며, 49.2%가 재미있었다고 하였고 이해 비해 16.0%만이 재미없었다고 하였다. 전체 대상자들의 치료요인을 보면 실존적 인자가 최상위를 차지하였으며, 다음으로 보편성, 치료자와 동일시, 희망의 고취가 순서대로 상위순위를 차지하였다. 전체 대상자를 순수관객, 상황극 참가자, 보조자아, 공유참가자의 4집단으로 나누어 치료요인을 비교해 보면, 순수관객의 치료요인 순위는 대체로 전체 집단의 치료요인 순위와 유사하였으며, 상황극 참가자의 경우 다른 구성원들에 비해 카타르시스가 약간 높은 순위를 차지하였고, 보조자아의 경우 다른 구성원들에 비해 구성원의 조언과 치료자의 조언이 상대적으로 높은 순위를 차지하였으며, 공유 참가자의 경우에는 다른 구성원들에 비해 집단응집력이 비교적 높은 순위를 차지하였다. 결 론 : 비행청소년의 수는 나날이 증가하고 있으나 예산 문제와 전문가의 부재 등으로 인해 이들을 위해 적절한 치료프로그램을 시행하기 어려운 실정이다. 본 사이코드라마는 대집단으로 시행되므로 비용면에서 경제적이며, 치료자가 한번에 많은 비행청소년에게 접근할 수 있어 시간적으로 효율적이라 할 수 있다. 그러므로 본 사이코드라마를 비행청소년을 위한 치료프로그램으로 적극적으로 활용할 필요가 있을 것으로 생각된다. Objectives : This study is designed to develop a large group phychodrama for light delin-quent adolescents (hereinafter called as "Psychodrama") thereby determining therapeutic factors for dealing with them. Methods : The study was conducted in the program of Seoul Metropolitan Youth Centre called "the class of love" during the period of July through December, 1998. The subjects consisted of a total of 827 young delinquents(759 boys and 68 girls, with the average age of 15.9 years old). To develop "this psychodrama", after we composed of therapeutic team centered by psychiatrists, we prepared from January to June, 1998 by practicing psychodrama with delinquent adolescents and by analyzing the results. "This psychodrama" has three basic steps: ① warming-up(small group meeting), ② action(situation drama and main drama), ③ sharing. A survey of treatments, from a pool of participating adolescents, based on evaluation sheets and 13 therapeutic factors scale followed every session of this psychodrama. Results : "This psychodrama" was helpful for 47.4%, interesting for 49.2%, not helpful for 12.1% and not interesting for 16%. In the whole group of subjects, the important therapeutic factors were existential factor, universality, identification with the therapist and the instillation of hope in the order of rank. When categorized in four different groups(audience, participants in situation drama, auxiliary egos, participants in sharing) the 4 groups displayed different results to one another. First, the therapeutic factors of audience were similar to that others. Third, auxiliary egos were leading in the guidance of members and therapists. Fourth, participants in sharing displayed higher group cohesiveness. Conclusion : As it is getting harder to find program's suitable for the treatment of increasing number of delinquents due to limited budget and experts, this psychodrama could be more cost-effective than any other measures.

Sulfamide유도체의 합성과 화학적 성질에 관한 연구 (Ⅱ) : 2-Arylethylsulfamide유도체와 1,1,3,3-Tetraethoxypropane의 반응 Reaction of 2-Arylethylsulfamide Derivatives

이지선,이채호 圓光大學校 基礎自然科學硏究所 1996 基礎科學硏究誌 Vol.14 No.2

산촉매하에서 N―(2―arylethyl)sulfamiide 또는 N―alkyl―N'―(2―arylethyl)sulfanide와 1,1,3,3―tetraethoxypropene의 반응으로 2―(2―aryethyl)sulfamide―2H―1,2,6―thiadiazine 1,1―dioxide와 N―(3―acro―leino)―N―alkyl―N'―phenethylsulfanide를 얻었다. N―(2―aryethyl)sulfamides and N―alkyl―N'―(2―arylethyl)sulfamides were treated with with 1,1,3,3―tetraethoxypropene to yield 2―(2―aryethyl)sulfamide―2H―1,2,6―thiadiazine 1,1―dioxide and N―(3―acro―leino)―N―alkyl―N'―phenethylsulfanides