Characterization of the European type of maternal lineage evident in extant Jeju native pigs

Byeong‐Woo Kim,In‐Cheol Cho,Moon‐Sung Park,Tao Zhong,임현태,Sung‐Soo Lee,Hee‐Bok Park,Moon‐Suck Ko,Jun‐Heon Lee,Jin‐Tae Jeon 한국유전학회 2011 Genes & Genomics Vol.33 No.2

Using a partial D‐loop sequence of mtDNA, an intensive analysis was conducted of the maternal lineages of Jeju native pigs (JNPs) from Korea. In total, 100 mtDNA sequences were obtained from Asian wild boars (AWBs), European wild boars (EWBs), Asian domestic pigs (ADPs), European domestic pigs (EDPs), and JNPs and were used for phylogeny and network analyses. Two distinct JNP groups - one (JNPA) in the Asian cluster and the other (JNPE) in the European cluster - were identified in the estimated phylogenetic tree and network. The maternal lineage of JNPE was the closest to that of EWB and a clear haplogroup was identified that shared an identical haplotype (hap04) among 15 individuals of JNPE and 2 individuals of EWB. A Landrace and an EWB shared hap03with a JNPE. EWB, Landrace, Large White, and Duroc formed two clear haplogroups with JNPE in a parsimonious medianjoining network analysis, suggesting that an obvious maternal contribution of EDP has occurred in JNPE in recent years. A pair‐wise mismatch analysis also indicated that JNPE may have experienced a sudden population expansion, suggesting a more recent establishment compared with the gradual population expansion of JNPA. The JNPE group therefore should be further evaluated in order to decide whether this group should be culled or accepted into further programs for maintenance of the JNP population as a pure breed.

Bupivacaine과 ropivacaine이 Xenopus oocyte에 발현된 HERG 전류에 미치는 영향

김국성,이규승,김효신,손숙진,이상도,김광진,전병화,김윤희,박진봉 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Bupivacaine is an amide-type local anesthetic widely used for regional anesthesia. Ropivacaine is developed as a less cardiotoxic alternatives to bupivacaine. In the present study, we have analyzed the effects of bupivacaine and ropivacaine on HERG currents expressed in Xenopus oocytes. Bupivacaine and ropivacaine(3∼1,000μM) blocked HERG currents in a concentration dependent manner. EC_(50) was 26.1±3.1μM(n_(R) 0.65±0.04) and 43.5±7.9μM(n_(H) 0.99±0.13) in bupivacaine and ropivacaine, respectively. Bupivacaine and ropivacaine did not affect the activation and deactivation kinetics of HERG channels. However, the drugs decreased the slope conductance measured from fully activated current-voltage relationship curves. These results suggest that bupivacaine and ropivacaine have a similarinhibitory effect on HERG channels, which could be a possible cellular mechanism of LQT or ventricular arrythmia by the drugs.

Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

저강도 펄스형 초음파를 이용한 개의 슬개골 탈구 치료 효과

조성진,김남수,김화영,김선균,이성호,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The effect of low-intensity ultrasonography on dislocation of the patella and fracture healing is controversial, and current management of that does not generally involve the use of ultrasound therapy. Nine patients with dislocation of the patella were treated with low-intensity ultrasonography(l5 mW/㎠, 1∼3.3 MHz) per 10-15 min/days, with an interval of 3 days during the last 3 weeks, at Family Animal Hospital(in Kunsan city). Ultrasound therapy may be beneficial to dislocation of the patella and healing. This finding is of considerable importance in that treatment with a low-intensity pulsed ultrasound signal may reduce healing time and could yield substantial cost savings and decreases in disability associated with delayed healing of dislocation of the patella.

세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

만성 간질환에 대한 DDB(Biphenyl Dimethyl Dicaboxylate)의 임상적 효과

이헌영,임의혁,김성걸,김진희,육은주,성자원,김병호,정형용,강대영,김영건 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

To investigate the effects of short-term administration of DDB(Biphenyl Dimethyl Dicarboxylate) for decreasing elevated serum aminotransferase levels in chronic liver diseases, authors applied 3 capsules of DDB per day at each meal time for 35 patients and compared with 35 patients treated with other conservative management. The following results were obtained. 1. Serum alanine aminotransferase (ALT) levels significantly decreased from 247.1± 180.0 IU/L of pretreatment level to 96.6±126.0 IU/L, 61.1±62.7 IU/L and 38.0±26.8 IU/L after 1, 3 and 6 months of treatment(p<0.05) and effective rates were observed in 60.0%, 71.4% and 88.6% of patients after 1, 3 and 6 months of treatment. 2. Serum aspartate aminotransferase (AST) levels decreased also significantly from 167.8±96.9 IU/L of pretreatment levels to 124.4±155.0 IU/L, 77.9±49.6 IU/L, 60.6± 46.9 IU/L after 1, 3 and 6 months of treatment(p<0.05), but decreasing pattern of serum AST was less significant than that of serum ALT. 3. No significant difference were observed in the serum ALT and AST changes according to the cause and duration of hepatitis. 4. No significant adverse effects were observed in whole patients treated with DDB. It is suggested that small dose administration of DDB is effective for decreasing serum aminotrans ferase levels in chronic liver disease in which other conservative management was not effective.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

교육사회학의 연구 동향 분석

김경식,권민석,김병규,박형진,송승면,황정희 경북대학교 사범대학부속 중등교육연구소 2005 중등교육연구 Vol.53 No.3

한국, 일본, 미국, 영국의 교육사회학 학술지 논문을 주제별 내용분석을 통해 각각의 하위개념을 도출하고 개념의 유사성의 정도에 따라 다시 상위개념으로 분류하여 교육사회학의 주요 연구영역을 탐색하였다. 학술지에서 가장 많이 다루어진 영역은 교육과 평등/지위획득, 교육정책과 제도, 교육사회학의 성격/이론, 학교사회와 학업성취, 페미니스트(여성), 청소년 문제와 문화, 교사교육/교직사회, 공교육의 문제/교육개혁 등의 순으로 나타났다. 특히 교육정책과 제도 문제는 한국과 일본에서, 교육사회학의 발달과 정체성 문제는 영국에서, 학업성취 관련 내용은 미국에서 가장 많이 다루어졌다. The purpose of this study was to explore the research trend in sociology of education. To conduct the purpose of this study, the Korean Journal of Sociology of Education(KJSE), the Journal of Educational Sociology(JES), Sociology of Education(SOE), and Bitish Journal of Sociology of Education(BJSE)'s articles were analyzed. The findings of this study were as follows: First, though the major categories(major domains) of research areas were a few differences among nations, they were education & equality, educational policy, sociology of education theory, school society & academic achievement, feminism, adolescent problem & culture, teacher education, school crisis & education reform, course education, sociology of curriculum, and etc. Second, the most frequent research area was the educational policy & system problems in Korea and Japan; the development and identity of sociology of education in Britain; The school society & academic achievement in U.S.A.

장수군 주민의 핵심역량 수준 분석

조영숙,김병진,황대용,강경하 한국농업교육학회 2008 농업교육과 인적자원개발 Vol.40 No.3

이 연구는 농촌사회 속에서 살아가는 성인들의 삶의 현장 속에서 직업을 갖고 살아가는데 주어진 과제를 성공적으로 수행하기 위해 펼요한 핵심역량 수준을 진단하고 개인 특성에 따른 핵심역량 보유수준의 차이를 분석함으로써 핵심역량 향상을 위한 시사점을 탐색하고 제언하는 데 목적이 있다. 이를 위해 선행연구를 바탕으로 개발한 농촌주민의 핵심역량 설문지를 가지고 층화무작위추출(One-Staged Stratified Random Sampling)과 체계적 표집방법을 사용하여 선정된 600명의 장수군 농촌주민을 대상으로 핵심역량을 측정하였다. 이 연구의 주요 결과는 다음과 같다. 첫째, 장수군 농촌주민의 핵심역량은 전반적으로 직업기초능력이 가장 높았고, 그 다음으로 시민의식 능력, 기초문해능력, 직업전환능력, 전문직업능력, 여가문화능력 순으로서 전문직업능력과 여가문화능력이 낮게 나타났다. 따라서 농업기술센터 등 교육기관에서는 전문직업능력과 여가문화능력에 대한 교육 기회 확대나 강화가 펼요하다. 둘째, 6개 핵심역량 보유수준은 직업교육 경험이 없는 층보다 경험있는 층이, 비농가보다 농가가, 여자보다 남자가, 40대 이하와 60대 이상보다 50대에서, 저학력층보다 고학력층, 주부, 무직보다는 농업과 직장인 층에서, 소농보다는 대농과 중농에서 더 높은 것으로 나타났다. 따라서 농촌지역 성인의 현재 직업기초능력 수준을 정확히 진단하고 대상층의 특성과 요구를 명확히 파악하여, 그 결과를 토대로 교육프로그램을 개발하고 제공하는 노력이 필요하다. 이상의 연구결과를 바탕으로 몇 가지 제언을 하였다. 국어 및 컴퓨터 활용 교육 등 기초문해능력 향상을 위한 교육프로그램 개발 및 운영 강화, 자기주도적 학습능력, 문제해결능력 등 핵심역량 향상을 위한 교육프로그램의 개발·운영 등이 필요하다. The purpose of this study was to find core competencies necessary for successful performance of tasks given to rural resident in the age of human resource development and to determine whether the level of core competencies were different according to individual background. For these purpose, we analyzed domestic and foreign literature, and derived core competencies required to rural adult. We surveyed rural resident's perception about their level of vocational competencies. We conducted a questionnaire survey of 600 rural residents living in Jangsu gun sampled by One-Staged Stratified Random Sampling and systematic sampling. The result of this study was summarized as follows. First, rural residents' level of core competencies were generally low. Out of core competencies, basic vocational competency is high, professional competency and leisure culture competency is low. Second the level of core competencies was perceived highly by experienced vocational education, farm house, male, 50's, high educated, farmer and worker, large and middle size farm house. Thus the opportunity for developing the core competencies for these the vulnerable have to be enlarged. Based on the results of this study, we proposed several tasks to be promoted as a part of regional human resources development in Jangsu Gun: 'develop and strengthen education program for basic literacy competency such as literacy and computer', 'expand the opportunity to develop professional competency and leisure culture competency'.

경부 종괴의 초음파 소견

김진환,김충현,이병철 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

The 48 sonograms of 46 patients with neck masses were retrospectively reviewed to assess the superiority of ultrasonography. Neck masses were divided into salivary glands, thyroid gland, cervical lymph node origin masses, and others. The salivary gland masses(16 cases), included pleomorpic adenoma(5 cases), sialadenitis (9 cases), and abscess (2 cases). The thyroid gland masses(7 cases), included thyroiditis(2 cases), goiter(4 cases) and malignant tumor(1 case). The cervical lymphadenopathy(10 cases) included Inflammatory(7 cases) and neoplastic(3 cases). The other masses included branchial cleft cyst(2 cases), thyroglossal duct cyst(2 cases), cystic hygroma(2 cases), abscess(2 cases) etc. Real-time ultrasonography with water bag adapted linear array 5.00 MHz transducer enabled us to ascertain mass character in detail. Sonography is very useful for differentiating a soild lesion from a cystic one, for differentiating an intraglandular lesion from a superficially located lesion extrinsic to the gland. On the other hand, CT is the method of choice for seperating lesions of the deep portion of the neck from adjacent structures. The sonography does not visualize the entire deep portion at the neck but it is not a major drawback of the method because majority of lesion originate from the superficial portion