Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

고관절 반 치환술의 대퇴 골절 예방을 위한 강선 고정의 효과

유정한 ( Jeong Han Yoo ),박용욱 ( Yong Wook Park ),박진수 ( Jin Soo Park ),노규철 ( Kyu Cheol Rowe ),정국진 ( Kuk Jin Chung ),김홍균 ( Hong Kyun Kim ),김형년 ( Hyong Nyun Kim ),임희준 ( Hee Joon Lim ),이철 ( Cheol Lee ),황지효 ( 대한고관절학회 2009 Hip and Pelvis Vol.21 No.2

목적: 무시멘트형 양극성 반 인공 관절 치환술에 있어서 수술 도중 발생하는 대퇴 골절 예방을 위한 예방적 강선 고정술의 효과를 분석하고자 한다. 대상 및 방법: 2004년 1월부터 2008년 8월까지 무시멘트형 양극 인공 관절 치환술을 받은 65세 이상의 환자 48명(1군)을 대상으로 하여 강선을 사용하지 않은 48명의 환자(2군)와 비교하였다. 평균 나이는 75.4세(1), 73.6세(2)이었고 평균 추시 기간은 19.1개월(1), 18.3개월(2)이었다. 수술 도중 골절 발생율 외에 수술 시간, 수술 도중 예상 출혈량, 입원 기간, 방사선학적 안정성을 측정하였고 임상적 평가를 위한 대퇴 통증과 Jensen의 기능 점수와 Parker와 Palmer의 활동 점수를 비교하였다. 결과: 1군에서는 수술 도중 골절이 발생하지 않았으며 2군에서 4예(8.3%)가 발생하였다(p=0.041). 수술 시간은 각각 172분(1), 162분(2) 예상 출혈량은 866 cc(1), 855 cc(2), 입원기간 36일(1), 35일(2)이었다. 방사선학적으로 침강은 1.59 mm(1), 1.67 mm(2)이었으며 임상적으로 대퇴 통증이 1예(1), 2예(2) 관찰되었다. 임상적으로 Jensen의 기능 점수는 평균 2점(1) 2.2점(2), Parke와 Palmer의 활동 점수는 평균 5.2점(1), 5.3점 (2)이었다. 결론: 무시멘트형 인공 관절 치환술에 있어서 예방적 강선 고정술은 골절률을 의의있게 감소시켰다. Purpose: The purpose of this study was to assess the effect of a prophylactic cable fixation for prevention of femoral fractures in cementless bipolar hemiarthoplasties. Materials and Methods: Forty-eight cementless bipolar hemiarthroplasties with prophylactic cable fixations on the femur were performed in patients>65 years of age between January 2004 and August 2008 (group 1). The control group which did not undergo prophylactic cable fixation included 48 cases (group 2). The mean age was 75.4 years (group 1) and 73.6 years (group 2). The patients were followed up for an average of 19.1 (group 1) and 18.3 months (group 2). The intra-operative fracture rates were compared. Additionally, operative time, estimated blood loss (EBL), and length of hospital stay were compared. Radiologic assessment for stem stability was performed. Clinical assessment was evaluated by the thigh pain and Jensen`s functional score, and Parker & Palmer`s mobility score. Results: There was no fractures in the cabled group (1), and 4 fractures (8.3%) in the control group (2; p=0.041). The mean operative time was 172 minutes (1) and 162 minutes (2), the EBL was 866 cc (1) and 855 cc (2), and the duration of admission was 36 (1) and 35 days (2), respectively. Radiologically, subsidence was 1.59 mm (1) and 1.67 mm (2). Clinically, one (1) and two (2) thigh pains were recorded and the functional score of Jensen was 2 (1) and 2.2 points (2), and the mobility score of Parker and Palmer was 5.2 (1) and 5.3 points (2), respectively. Conclusion: Prophylactic cable fixation is effective for reducing intra-operative femoral fractures.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.

좌전하행지 관동맥 개구부위의 망사형의 관(Slotted Tube)스텐트와 나선형 코일(Coil)스텐트 삽입후 단기 및 장기 암상 경과의 비교

박훈기,박성욱,이일수,이상곤,김진우,이근,정상식,이철환,홍명기,김재중,박승정 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.6

회전근 개 병변에서 골 단일광자방출전산화 단층촬영과 단순 방사선 사진의 비교

박진영,박선건,김승철,임종범 단국대학교 2000 論文集 Vol.35 No.-

purpose : The increased uptake on the bone scan represents the zone of increased bon metabolism. Single photon emission computerized tomography(SPECT) is more precise in detecting lesions of increased uptake than planar bone scan. The purpose of this study is to examine applicability of bone SPECT to shoulder lesions and to analyze the efficacy of SPECT compared with simple X-ray in rotator cuff lesion. Materials and Methods : Subjects were 21 patients with of rotator cuff lesion confirmed by surgical(arthroscopic or open) procedure. Increased uptake(bone SPECT) and sclerosis of greater tuberosity and anterior acromion on X-ray were considered positive. Results : For acromion, sensitivity of the bone SPECT was 90.9% and specificity was 50.0%. For greater tuberosity, sensitivity was 92.3% and specificity 37.5%. For acromioclavicular joint, sensitivity was 80.0% and specificity 81.3%. Conclusion : This study demonstrates that the uptake of radioisotope in shoulder is increased in patients with painful partial or total rotator cuff tear. And extrinsic mechanical pressure seems to be the cause of rotator cuff lesion of the shoulder.

Destructive Encephalopathy를 보인 Incontinentia Pigmenti 1예

한진규,최재철,박민규,박건우,은백린,정지태,이대희 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

김치발효중 유기산 및 당함량변화

朴哲辰,曺哉銑 경희대학교 부설 식량자원개발연구소 1989 硏究論文集 Vol.10 No.-

In order to investigate the changes in sugar and organic acids of kimchi during fermentation, Chinese cabbage, radish and kimchi extracts were fermented by lactic acid bacteria and then analyzed acidity, pH, sugar and (acidic ac) of fermented samples. The major organic acids in the samples were lactic, acetic, malic and succinic acid Of these acids, lactic, acetic and citric acid were increased but malic and succinic acid were decreased during kimchi fermentation. The major sugars of the samples were fructose and glucose. The amount of sugars in these samples were decreased more rapidly when fermented at higher temperature than that of low temperature.

김치발효중 유기산 및 당함량변화

朴哲辰,曺哉銑 慶熙大學校 1989 論文集 Vol.18 No.-

In order to investigate the changes in sugar and organic acids of kimchi during fermentation, Chinese cabbage, radish and kimchi extracts were fermented by lactic acid bacteria and then analyzed acidity, pH, sugar and ( acid ic ac ) of fermented samples. The major organic acids in the samples were lactic, acetic, malic and succinic acid. Of these acids, lactic, acetic and citric acid were increased but malic and succinic acid were decreased during kimchi fermentation. The major sugars of the samples were fructose and glucose. The amount of sugars in these samples were decreased more rapidly when fermented at higher temperature than that of low temperature.

시판 항생제들의 대추나무빗자루병 치료약제 가능성 비교

박철하,이세표,차병진 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

To minimize the trial and error by farmers who have no other choice but to use antibiotics for human-disease control, because no agricultural tetracycline for jujube witches-broom disease is available at this point. therapeutical efficacy of several commercial antibiotics against the disease through trunk injection was evaluated. The efficacy of human tetracyclines in trunk injection of jujube tree for witches-broom therapy varied to some extnet. Tetracyclines for intravenous or subepidermal injection showed the strongest symptom-suppression effect. Those, however, were not practical because of high price. On the other hand, tetracyclines in capsule were much cheaper than the former and can be used practically. For trunk injection, just the powder in capsule should be dissolved in clean water and then filtered through cheese cloth or tissue papaer to remove the sediments unnecessary for trees. Otherwise, the sediment would clog the injection hole and the feeder tube. In this experiment. tetracycline HCl of Glaxo made the least sediment in the solution. In addition, the plastic bottle to contain Korean wine was much cheaper and more practical than the commercial one for tree injection.