Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

고등학교 1학년 학생들의 인지수준과 과학의 물질 단원 내용수준 비교 분석 및 교사들의 인식

김성숙,박소영,정미현,류상미,강성주,박국태 韓國敎員大學校 敎育硏究院 2009 敎員敎育 Vol.25 No.4

The purpose of this study was to seek for improved teaching and learning methods by comparatively analyzing the cognitive level of 10th grade students and the content level of high school science textbooks's matter unit and examining the teacher's understanding of these issues. For this study, the cognitive level of 336 10th grade students from 4 areas were analyzed using SRT Ⅲ (Science Reasoning Task Ⅲ) and the content level of 10 scientific concepts selected from the matter unit of high school science textbooks were analyzed using CAT (Curriculum Analysis Taxonomy). How high school science teachers understood these issues was also examined. The result showed that 51% of the research subjects were the formal operational level, 30% of the transitional level, and 19% of the concrete operational level. The percentage of students of the formal operational level varied according to each school. The scientific concepts of the matter unit of the 6 high school science textbooks were mostly of the early formal operational level. However, the content level of the same scientific concept varied according to each textbook, since the level of each textbookordiexpdeining scientific concepts to students and the answers theyiexpected from students were different. Furthermore, high school science teachers considered the content level of the scientific concepts lower than the actual content level analyzed through teptsAT. Such d6 harmonyibetween the cognitive level of students and the content level of the scientific concepts and the wrong conceptt. Furtteachers of the content level of the scientific concepts effects the students' understanding of scientific concepts and teaching-learning methods. I Furder to solve the discordance between the level of students and scientific concepts and to increase the understanding of students of scientific concepts, teachers should develop the appropriate teaching and learning methods that considers the cognitive level of students and content level of scientific concepts, by analyzing the cognitive level of students and understand the content level of scientific concepts of science textbooks, prior to class. 이 연구는 고등학교 1학년 학생들의 인지수준과 고등학교 과학 물질 단원의 내용수준을 비교 분석하고, 이에 대한 공통과학 교사들의 인식을 알아봄으로써, 교수 학습 방법에 대한 시사점을 얻고자 하는 것이다. 연구를 위하여 4개 지역의 고등학교 1학년 학생 336명 을 대상으로 SRT Ⅲ(science reasoning task Ⅲ)를 사용하여 인지수준을 조사하였으며, 고등학교 과학 물질 단원에서 10개의 과학 개념을 추출하여 CAT (curriculum analysis taxonomy) 분석틀로 내용수준을 분석하였다. 그리고 이에 대한 공통과학 교사들의 인식을 조사하였다. 연구 결과에 의하면, 고등학교 1학년 학생들의 51%가 형식적 조작가 수준이었으나, 30%의 학생들과 19%의 학생들은 각각 과도기 수준과 구체적 조작기 수준이었으며, 형식적 조작기 수준의 학생 비율은 학교별로 차이가 있었다. 고등학교 6종 과학교파서에 제시된 물질 단원 과학 개념들의 내용수준은 대부분 초기 형식적 조작기 수준이었으니, 같은 과학 개념이라도 설명하는 수준과 학생들에게 요구하는 답변의 수준 차이로 인해 교과서별로 내용수준에 차이가 있었다. 그리고 과학교사들은 과학 개념의 내용수준을 CAT 분석틀로 분석된 실제의 내용수준에 비해서 낮게 인식하고 있었다. 이러한 학생들의 인지수준과 과학 개념의 내용수준의 불일치와 과학교사들의 과학 개념 내용수준에 대한 잘못된 인식이 학생들의 과학개념에 대한 이해도와 교수-학습에 영향을 미칠 것이다. 그러므로 학생들의 인지수준과 과학 개념 내용수준간의 불균형을 해소하고 과학 개념에 대한 학생들의 이해도를 높이기 위해서는 과학교사들이 학생들의 인지수준과 과학 개념의 내용수준을 사전에 파악하여, 학생들의 인지수준과 파학 개념의 내용수준을 고려하는 교수-학습 전략이 필요하다.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

개에서 발생한 기관 허탈의 진단과 관리

이새미,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 11-year-old Yorkshire terrier anesthetized for computed tomography. The dog showed cyanosis and syncope during postanesthetic recovery, therefore drug administrated parenterally. Goose-honking was noticed after full recovery. As a result of survey radiography and fluoroscopy, grade 4 tracheal collapse was diagnosed. Prescription for the dog was oral administration of hydrocodone 0.22 mg/kg. theophylline 20 mg/kg, prednisone 0.5 mg/kg, amoxicillin 22 mg/kg and chlorpheniramine 2 mg/kg twice a day for 3 days. A 10-year-old Pomeranian was diagnosed as grade 2 traceal collapse at the local animal hospital 2 years before being referred to the veterinary teaching hospital of Chungnam National University. On physical examination, goose-honking, coughing and intermittent open-mouth breathing. Tracheal narrowing was observed on survey einradiography and fluoroscopy. Oral administration of theophylline 13 mg/kg, enrofloxacin 5 mg/kg, chlropheniramine 0.5 mg/kg twice a day was prescribed for 2 weeks.

토착형 P. vivax에 의한 Malaria 4례

박형렬,이성원,이경미,김태겸,한진영,손춘희,정원태,김성은,김종성 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.2

고콜레스테롤 혈증 환자에서 Cerivastatin(LIPOBAY, 의과학연구소)의 임상효과 및 안전성에 관한 연구

박성훈,김미연,정수진,이영숙,박창한,변은경,전성희 梨花女子大學校 醫科大學 醫科學硏究所 2001 EMJ (Ewha medical journal) Vol.24 No.3

목적 : Cerivastatin(LIPOBAY)은 최근에 개발되어 사용되기 시작한 HMG-CoA 환원효소 억제제로서 저용량으로 탁월한 콜레스테롤 강하효과를 보이는 약물이다. 저자들은 Cerivatatin(LIPOBAY)의 혈중 콜레스테롤 강하효과를 평가하고 약물의 안전성을 평가하기 위하여 본 연구를 시행하였다. 방법 : 고콜레스테롤혈증 환자 37명(남자 13명, 여자 24명)을 대상으로 하였다. 대상환자는 2주간의 저콜레스테롤 식이 후에도 혈청 콜레스테롤이 240㎎/㎗이상이거나, 관상동맥질환이 있는 환자는 혈청콜레스테롤이 220㎎/㎗이상인 경우 cerivastatin 0.4㎎을 8주간 복용하도록 하였다. 임상적 관찰과 검사실 검사(간기능검사, 일반혈액검사, 혈청지질검사)는 투약개시 후 4주 및 8주 후에 시행하였다. 결과 : Cerivastatin 0.4㎎을 4주간 투여한 후 측정한 LDL 콜레스테롤, 총콜레스테롤, triglyceride, 및 HDL 콜레스테롤은 각각 38%, 28.8%, 11.6% 및 7.8% 감소하였다. 총콜레스테롤/HDL 콜레스테롤 비 및 LDL/HDL비는 각각 20.8% 및 31.1% 감소하였다. 투약 8주후에 측정한 검사소견은 4주후에 비해 유의한 변화를 보이지 않았다. 한명의 환자(2.7%)에서 간기능 검사상 AST, 및 ALT의 지속적인 상승으로 투약을 중지하였다. 결론 : Cerivastatin 0.4㎎/day는 혈청콜레스테롤을 효과적으로 감소시켰으며 심각한 부작용은 볼 수 없었다. 따라서 적극적으로 LDL콜레스테롤을 낮출 필요가 있는 고콜레스테롤혈증 환자에서 cerivastatin은 효과적이고 안전한 약물이라고 생각된다. Objectives : Cerivastain(LIPOBAY) is recently developed HMG-CoA reductase inhibitor which is effective in lowering serum cholesterol levels at microgram does. We evaluated the clinical efficacy and safety of cerivastatin(LIPOBAY) in patients with hypercholesterolemia. Method : Thirty-seven patients(male : 13, female : 24) with hypercholesteolemia defined as baseline serum total cholesterol ≥240㎎/㎗, or ≥220㎎/㎗ in patients with known coronary artery disease were included for this study. After 2 weeks of low cholesterol diet, if the serum total choesterol level meet the criteria, cerivastain 0.4㎎/day was prescribed for 8 weeks. Clinical follow-up and laboratory tests were performed 4 weeks and 8 weeks after medication. Results : After 4 weeks of cerivastain 0.4㎎/day treatment, low density lipoprotein(LDL) cholesterol decreased 38% and total cholesterol decreased 28.8% from baseline. Triglyceride decreased 11.6%, and high density lipoprotein(HDL) cholesterol decreased 7.8% from baseline. Total cholesterol/HDL ratio decreased 20.8% and LDL/HDL ratio decreased 31.1% from baseline. After 8 weeks of treatment, no further significant changes were noted compared with the values at 4 weeks. Cervastatin was discontinued in one patient(2.7%) due to continuous liver enzyme elevation. Conclusion : Cerivastatin 0.4㎎/day is effective in lowering serum cholesterol levels without significant adverse reactions. Cerivastatin is effective and safe for patients with hypercholesterolemia who needs aggressive LDL cholesterol lowering.

개에서 발생한 양파 섭취에 의한 하인즈 소체 용혈성 빈혈 증례

이미선,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 10 month-old Yorkshire Terrier was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of lethargy, bile pigment in urine and anorexia. Blood smear showed Heinz body, polychromatocytosis, schistocytosis, anisocytosis, which were the results from the oxidative injury. Hematology demonstrated a moderate anemia(PCV 22.4%, reference rage 35-55%), which was regeneration based on the degree of RPI (4.03, reference rage > 1). Serum biochemistry demonstrated mild elevation in total bilrubin, and mild decrease in total protein and albumin concentration. A diagnosis of Heinz body hemolytic anemia secondary to onion ingestion was made and treated with fluid therapy, oxygen and antioxidant drugs. The dog recovered without any complication.

‘숙명역사관’뮤지엄 아이덴티티 개선을 위한 마케팅, 디자인 전략 수립 -특별 기획전, 홍보, 기프트 샵을 중심으로-

박민지,박한빛,서예원,이진민,장미정,이성애 숙명여자대학교 산업디자인연구소 2017 숙명디자인학 연구 Vol.24 No.-

본 연구는 디자인과 마케팅이 이분법적으로 나누어질 수 없음을 인식하고, 디자인과 마케팅을 융합하여 스페이스 디자인 마케팅을 통한 뮤지엄아이덴티티를 구축하는 일련의 프로세스를 제안하는데 목적이 있다. 본 연구는 2017년 3월~6월 총 15주간 숙명여자대학교 환경디자인과 ‘브랜드·스페이스·마케팅’수업에 서 진행된 결과물로서 작업물을 정리하였다. 본 연구의 범위는 4개(物, 事, 人, 場)의 주제 중, 장소(場)를 중심으로 ‘숙명역사관’의 그래픽, 제품, 공간을 개발하는 브랜드 매뉴얼과 연관하여 한정한다. 이에 본 연구는 ‘숙명역사관’이 가지고 있는 역사적 컨텐츠와 가치를 통해 현재 상황과 문제점을 분석하였다. 그 결과 ‘숙명역사관’의 다양한 마케팅 전략을 제시하고 이에 따른 디자인 전략과 해결 방안을 통해 디자인과 마케팅의 융합을 꾀하였다. 아울러 SWOT 분석을 통해 로고, 명함부터 새로 기획하는 숙명역사관 기프트샵, 인테리어디자인, 디스플레이, 공간연출과 그 안의 제품디자인에 이르기까지 일관성과 통일성을 추구했다. 끝으로 본 연구는 뮤지엄아이덴티티 구축을 위한 창의적 디자인 프로세스를 고찰하며, 이를 바탕으로 개발 매뉴얼을 총체적으로 정리함에 있어서 뮤지엄 브랜딩의 기초 자료로 활용될 것으로 기대된다.