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For every 9kg of biodiesel production, about 1kg of crude glycerol is generated as a byproduct. Glycerol is a good carbon source for the production of 3-hydroxypropionic acid (3-HP). Several engineered microbes such as Escherichia coli, Klebsiella pneumoniae and Pseudomonas denitrificans have been developed for the production of 3-HP by introducing an aldehyde dehydrogenase and a glycerol dehydratase. However, both the enzymes were seriously inactivated during the reaction. To minimize the inactivation of the enzymes, balanced expression of the genes was attempted by promoter engineering and unique dynamic promoters which are induced by 3-HP have been employed. The dynamic promoters could express the genes of the two enzymes continuously and maintained their activity until the end of fermentation. This talk summarizes our long efforts for the 3-HP production and discusses the potential and limitations of our work. Also, glycerol metabolisms affecting 3-HP production are discussed.
Three new acylhomoserine lactone (AHL) inhibitors have been isolated from the Korean red alga, Ahnfeltiopsis flabelliformis, via bioactivity-guided fractionation using the recombinant Agrobacterium tumefaciens liquid culture bioassay. Unlike the majority of AHL inhibitors reported to date, these compounds were α-D-galactopyranosyl-(1→2)-glycerol (floridoside) (1), bet-onicine (2), and isethionic acid (3), all of which are structurally unrelated to AHLs.
Branchial cleft cysts and branchial anomalies develop from the branchial cleft apparatus that persists after fetal development. The most common anatomical site for the occurrence of branchial cleft cysts is in the cervical area, generally anterior to the sternomastoid muscle in the upper or middle portion of the neck. A mediastinal branchial cleft cyst is extremely rare and few cases have been reported. We report the case of branchial cleft cyst found in the anterior mediastinum with literature review.
We prepared (Bi2Te3)x (Sb2Te3)2-x p-type thermoelectric materials via SPS(spark plasma sintering) technique and the thermoelectric properties have been investigated by using Harman method at RT. With the increase of Sb2Te3 content, the electrical resistivity and Seebeck coefficient decreased while the thermal conductivity increased. The maximum z value of the sintered thermoelectric material was 3.2 at RT. Also, p-type Bi2Te3-based ingots with 50mm in diameter were prepared by SPS technique. To verify the reliability of the ingots, thermoelectric and mechanical properties such as Seebeck distribution and density were studied. Finally, thermoelectric module with the dimension of 40mm X 40mm was fabricated by using p-type materials by SPS and n-type materials by zone melting technique. The Z value of the module was 2.7 at RT.