들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

정신분열병과 Neurotensin 수용체 유전자 다형성의 연합 연구

이유상,김형배,한진희,채영규,이정식,이혜순,주연호,김형섭,최인근,양병환 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: Neurotensin(NT)은 NT수용체와 결합하여 그 효과를 나타내는 neuromodulator 혹은 neurotransmitter로서 대뇌에서 도파민의 분비를 조절하는데 중요한 역할을 한다. 근래의 연구에 의하면 NT와 그 수용체는 대뇌에서 항정신병 약물의 효과를 매개하는 것으로 생각되고 있으며 약물치료를 받지 않은 정신분열병 환자의 뇌척수액에서 NT의 양이 적으로 보고되고 있어 이들은 정신분열병과 깊은 관련을 가지고 있을 것으로 추정된다. 최근 NT수용체의 유전자의 3`인접영역에서 정보가치가 높은 4 염기반복 다형성이 발견되어 이를 유전 표지자로 하여 정신분열병과의 연합을 알아보았다. 방 법: 서로 혈연관계에 있지 않은 정신분열병 환자 120명(남자 91명, 여자 29명)과 정상 대조군 106명(남자 84명, 여자 22명)을 대상으로 하였다. PANSS를 사용하여 양성 및 음성을 알아보았다. 말초혈액에서 DNA를 분리한 후에 중합효소연쇄반응을 사용하여 3`인접영역에 있는 4 염기 반복 다형성을 증폭하였고 silver staining한 후에 유전자형을 관찰하였다. chi-square 검증과 Bonferroni`s correction을 사용하여 환자군과 정상 대조군간의 대립유전자 빈도의 차이를 알아보았다. 또한 양성 및 음성 환자군으로 나누어 차이를 알아보았다. 결 과: 총 23개의 대립유전자가 관찰되었으며, 399bp의 대립유전자(A10)의 빈도가 환자군보다 정상대조군에서 통계적으로 유의하게 높았다(χ²=16.49, df=1, p<0.001). 음성 정신분열병 환자군과 정상대조군 사이의 비교에서는 정상대조군의 A10의 빈도가 환자군보다 유의하게 높았다(χ²=21.33, df=1, p<0.001). 성별 비교에서 남자 정신분열병 환자군은 대조군에 비하여 A10의 분포가 유의하게 적었다. (χ²=13.71, df=1, p<0.001) 결 론: NT 수용체 유전자와 정신분열병사이에 음성연합이 관찰되었다. NT 수용체 유전자가 일부 정신분열병의 발병과정에서 확실하지는 않지만 어떤 종류의 보호기능을 할 수도 있다는 것을 암시한다. Objectives: Neurotensin(NT), of which functions are evoked by its interaction with neurotensin receptors(NTR), coexists with mesolimbic dopamine and regulates endogenous dopamine release. Recent studies have shown that NT with NTR exerts neuroleptic-like activity within the central nervous system and may play an important role in the pathogenesis and in the treatment of schizophrenia. We have examined the gentic association between schizophrenia and tetranucleotide repeat polymorphism in the 3-flanking region of the NTR gene to investigate the possible contribution of the NTR gene to the schizophrenia susceptibility. Methods: Among 23 alleles identified, the subjects were 120 patients(male 91, female 29)with schizophrenia and 106 normal healthy controls(male 84, female 22). They were unrelated native Korean. PANSS was used to determine positive or negative subgroup in the schizophrenic patients. Using polymerase chain reaction and polyacrylamide gel electrophoresis, tetranucleotide repeat polymorphism(CCTT and CTT) in the 3`-flanking region of NTR gene was observed. For a comparison of NTR gene`s allelic frequencies between patients with schizophrenia and normal healthy controls, chi-square test and Bonferroni`s correction was performed. Results: The frequency of A10 allele(base pair size=399)was significantly higher in normal healthy controls than schizophrenia(χ²=16.4902, df=1, p<.000). In the comparison between schizophrenic patients with negative symptoms and normal controls, the frequency of A10 allele was significantly higher in normal healthy control subjects than patients with schizophrenia(χ²=21.33, df=1, p<0.001). In the case of male, the frequency of A10 allele of schizophrenia was significantly higher than normal controls(χ²=13.71, df=1, p<0.001). Conclusions: NTR gene was negatively associated with schizophrenia. NTR gene`s tetranucleotide repeat polymorphism may provide some protective function against schizophrenia.

等電點 電氣泳動에 의한 배추와 양배추 育成系統에서의 自家不和合性 關聯 glycoproteins의 比較分析

이정명,최근원,민병훈,이태진,백남권,이철우 경희대학교 부설 식량자원개발연구소 1994 硏究論文集 Vol.15 No.-

等電點 電氣泳動을 이용하여 배추의 몇 개의 5-allele 造成과 그들의 F₁에서 自家不和合性의 발현과 관련된 glycoprotein의 band 樣相을 비교 분석한 결과는 다음과 같다. 1. 배추의 內婚系와 이들 간의 에서 柱頭內 glycoprotein의 band pattern을 分析한 결과 兩親의 band pattern이 F₁으로 그대로 遺傳되므로 써 명확한 同定 및 區分이 가능하였다. 2. 공시된 배추의 계통 중에서 8개의 等電點을 발견하였는데 대부분의 系統에서 pl 7.0 또는 8.6에서 major band를 보이고 있었다. 따라서 major band만으로는 S-allele genotype의 명확한 동정이 불가능한 경우도 상당히 있었다. 3. 상기 bands (pI 6.8 and 7.7)에서의 glycoprotein의 分子量은 pI 7.0에서 49,000-50,000이었고 pI 8.6에서 59,000으로 推定되었다. 4. 양배추는 배추에 비해서 glycoprotein의 major bands와 minor bands수가 많고 매우 다양하게 나타나므로써 S-allele 동정이 배추보다 매우 용이할 것임을 보여 주었다. Band patterns of glycoproteins associated with the expression of self-incompatibility were examined in the stigmatic extracts of self-incompatible Chinese cabbage inbred lines and the hybrids. Clear relationship in the band pattern could be confirmed in the parents and their hybrid, indicating the inheritance of S-alleles to the progeny. Total of 8 isoelectric points were indentified in the inbreds, with the major bands at pI 7.0 and 8.6. Thereforne, in some genotypes, identification of cultivars or S-allele genotypes were impossible with the major bands only. The molecular weights of the glycoproteins were estimated to be 49,000-50,000 for pI 7.0 and 59,000 for pI 8.6 bands. The stigmatic extracts from cabbages showed numerous major and minor bands so that identification of S-allele genotype will be much easier as compared to chinese cabbage.

수모세포종과 대뇌 원시 신경외배엽 종양의 면역조직화학적 연구

이정일,왕규창,지제근,조병규 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.8

Although it is becoming widely accepted that medulloblastoma and cerebral primitive neuroectodermal tumor(PNET) are identical tumors occuring at different locations, there are some controversies in their origin and pathological classification. As a method of investigating whether the tumors are identical in pathological aspects, immunohistochemical characteristics of medulloblastomas and cerebral PNETs were compared in this study. Also the prognostic significance of the immunohistochemical findings in modulloblastoma patients was analyzed. Clinical features of twenty-seven patients with modulloblastoma and eleven patients with cerebral PNET were reviewed, excluding tumors with significant cellular differentiation such as ependymoblastoma, pineoblastoma and neuroblastoma. The presence of glial fibrillary acidic protein(GFAP), neurofilament(NF), S-100 protein, vimentin synaptophysin, and epithelial membrane antigen (EMA) was examined with immunohistochemical method and the differences of the results between the two tumors were statistically analyzed. The positive rates of NF and synaptophysin were significantly higher in medulloblastomas(p = 0.006 and 0.003, respectively) and so was the positive rate of vimentin in cerebral PNETs(p=0.004). S-100 protein showed a higher positive rate in cerebral PNETs although it was not statistically significant Univariate and multivariate analyses did not show any significant correlation between the duration of survival and the presence of cellular antigens.

정신분열병의 유병기간과 인지적 결함간의 관계

이근창,이종학,강병조 대한생물치료정신의학회 1996 생물치료정신의학 Vol.2 No.1

정신분열병에 대한 현행의 개념이 손상된 인지기능이 이 질환의 정신병리중의 하나라고 받아들여지고 있다. 그러나 정신분열병의 경과가 점진적이고 지속적인 손상을 유발하는 진행적인 치매적 장애인지 급성 또는 아급성과정에서 인지기능의 감소가 오고 결국은 비교적 안정된 결함 상태가 되는 정적뇌병증인지에 대한 논란은 아직도 계속되고 있다. 본 연구에서는 58명의 정신분열병환자들을 유병기간에 따라 5년이하(Group 1), 5년초과에서 10년이하(Group 2), 10년초과에서 15년이하(Group 3), 15년초과에서 20년이하(Group 41)의 4군으로 분류하여 정신분열병환자들에서 유병기간이 증가함에 따른 인지기능의 차이가 나타나는 지에 대해서 WCST, VCPT, 재인기억검사, 공간기억검사, 수지럭검사, KWIS 어휘문제 및 BPRS를 시행하여 다음과 같은 결과를 얻었다. 1) 각 군간에서의 간이정신평가척도(F=0.22, d.f.=3,54, P=0.88)는 유의한 차이가 없었다. 2) Wisconsin카드분류검사(F=0.23, d.f.=3,54, p=0.87), 시각지속수행검사(F=0.27, d.f.=3,54, P=0.84), 공간기억검사(F=0.10, d.f.=3,54, P=0.96), 재인기억검사(F=0.15, d.f.=3,54, p=0.93) 수지력검사(F=1.20, d.f.=3,54, p=0.32), 어휘문제(F=0.67. d.f.=3,54, P=0.98) 등 실시한 모든 신경심리검사에서 4군간에 유의한 차이가 없었다. 이러한 결과로 볼 때 정신분열병은 유병기간과는 무관한 정적뇌병증일 것이라는 것을 시사한다. Most current thinking about schizophrenia acknowledges that impaired cognitive performance is one of the manifestations of the neuropathology of the illness. But, controversy surrounds the issue of whether a dementing disorder which produces a gradual and progressive loss of cognitive function or a static encephalopathy which is a relatively stable deficit state followed after cognitive function diminishing during an acute or subacute process. In this study, 58 schizophrenic patients were assigned to four duration of illness-derived groups: duration of illness≤5years(group 1), 5<duration of illness≤10years(group 2), 10<duration of illness≤15years(group 3), 15<duration of illness≤20years(group 4). To ascertain the significant differences of the cognitive function between 4 duration of illness-derived groups, WCST, VCPT, recognition test, spatial memory, finger tapping test, KWIS-vocabulary test, and BPRS were performed. The results are as follows ; 1) There was no significant differences between 4 groups on BPRS(F=0.22, d.f.=3,54, P=0.88). 2) There were no significant differences between 4 groups on WCST(F=0.23, d.f.=3,54, P=0.87), VCPT(F=0.27, d.f.=3,54, P=0.84), spatial memory(F=0.10, d.f.=3,54, P=0.96), recognition test(F=0.15, d.f.=3,54, P=0.93), finger tapping test(F=1.20, d.f.=3,54, P=0.32), KWIS-vocabulary test(F=0.67, d.f.=3,54, P=0.98). These results suggest that the course of schizophrenia is more consistent with a static encephalopathy than a dementing disorder, irrespective of duration of illness.

기-액계면 흡착에 의한 계면활성제 및 중금속분리

이근희,남상진,김병조,박경기,박종태,신정호 釜山大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.53 No.-

CTAB에 의한 CuS 침전 미립자의 응집과 기포 흡착특성을 고찰하였다. CTAB의 기포흡착은 Langmuir흡착식을 따르며, 포말간 본체액의 동반을 고려한 회분해석으로부터 구한 흡착열은 3700cal/mol로 나타났다. CTAB에 의한 CuS미립자의 기포흡착은 기포-입자간 충돌흡착으로 설명되었고, 흡착분리에 대한 최적 농도비는 CuS의 최적 응집농도비와 일치하였으며, 그 값은 [CTAB] 대 [CuS]가 0.1로 얻어졌다. 기포에 의한 포집효율은 pH와 CTAB농도 등에 의존하나 공기의 유량에는 무관하였으며, 최대 포집효율은 최적 첨가농도에서 0.0002로 나타났다. 또한 Cu-Cd-Zn 황화물의 혼합계에서 CTAB를 사용한 기포흡착의 경우 ZnS의 선택적 분리가 가능하였다. The characteristics of the bubble adsorptive separation of CTAB(cetyltrimethyl- ammonium bromide) and CuS precipitates was investigated. The Langmuir adsorption equation was adequate at very low concentration of CTAB, and the adsorption heat was determined from the batch analysis considering the bulk liquid accompanied between bubbles. The adsorption mechanism was explained with the collision adsorption between bubbles and precipitate particles. The optimum concentration ratio of [CTAB] to [CuS] for adsorptive separation was 0.1 and coincided with the ratio for the coagulation of particles. The collection efficiency was depended on pH and CTAB concentration but not the air flow rate, and the maximum efficiency was 0.0002. The selective separation of ZnS from the mixture of Cu-Cd-Zn sulfides was able by the bubble adsorption with CTAB.

퍼지 환경하에서 유연생산시스템 일정계획의 Pairwise 해법에 관한 연구

이상완,신대혁,윤연근,박병주 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

FMS(Flexible Manufacturing System) scheduling problem is a complex system and a NP-hard problem. Thus it is natural to look for heuristic method. Here, we consider the multi-part production scheduling problem in a FMS scheduring of altemative routings. The problem in more complex if the processing time is imprecision. It requires suitable method to deal with imprecision. Fuzzy set theory can be useful in modeling and solving scheduling problems with uncertain processing times. Lee-Ki fuzzy number comparison method will be used to compare processing times that evaluated under fuzziness. This study propose heuristic algorithm solving FMS scheduling problem under fuzzy environment. In this algorithm, the pairwise comparision method is used to find proper alternative routing. On the basis of the proposed method, and example in presented.

한국 경호환경의 동향과 전망에 대한 분석

이상철,유송근,박준석,김병주 龍仁大學校 1998 용인대학교 논문집 Vol.15 No.-

It is neccesary to facilitate the developments of the systems, principles and instructions of guarding in order that the security guard be able to meet the needs of the environments of guarding regardless what kind of government system our country may be. The guard also need the nationality and brilliant brains rather than the political power so that the guarded be safe always and everywhere and all people in the country feel peaceful and safe, which requires it that every bodyguard investigate and develope the new technology of guarding through reasonable and scientific methods. And I think followings are necessary for the development and advance of future the Security guard : the strong relations with the related organizations, the education facilities for supporting good and strong bodyguards, the related information bureau with mood image against the internation terror croups, and professionalization for better adaption in the idustralized various society, and the department for study on the principles of guarding. In addition to those, it would be important to be equipped with enough amarments and strong wills. Anyway, the next 21th century requires big change of the everyone's opinions on guarding and it would not be impossible to provide complete guard with only the system of guard itself, so it needs everyone's help. Of course will it require the professionals of Information technology too, in the future information society. I suggest that it need to combine various functions and specialize each fuction of the security guard at the same time. Then it would be more easy to maintain the system to prevent the future dangers, so to acomplish the complete guarding system. As a conclusion, it would be possible to meet the 21th environment of guarding only with the scientific, reasonable and specialized guard system.

뜬 바닥구조용 완충재의 동탄성계수 측정에 관한 연구

김하근,김명준,이병권 대한건축학회 2005 대한건축학회논문집 Vol.21 No.1

The aim of this study is to suggest the method of measuring the dynamic stiffness and the loss factor of materials used under floating floors in dwellings by Korea standard (KS F 2868) We measured and compared those of 18 resilient materials in several conditions such as the size of test specimens, the type of excitation methods, pick-up locations and so on in the first result, there's no differences of natural frequency according to vibration pick-up location in the second result, the dynamic stiffness by using sinusoidal signal of exciter is higher than that by using impulse signal of Impact hammer Finally, in case of measuring dynamic stiffness of material which has higher stiffness or is easy to change by bending moment, It should be measured with great care