토마토에 있어 PCR에 영향을 미치는 요인들

최근원,김현주 경희대학교 부설 식량자원개발연구소 1995 硏究論文集 Vol.16 No.-

토마토 에 있어 RAPD 분석을 위한 PCR에 영항을 미치는 요인들을 결정하기 위한 일련의 실험이 실시되었던 바, 최종 50㎕의 반응용액내에 25ng의 template DNA, 5pM의 10-mer primer, 0.05mM의 dNTP와 0.25 unit의 Taq DNA polymerase의 조건이 본 실험에 사용된 토마토의 PCR에 최적으로 사료되었다. 본 실험에서는 Operon사의 A_(9)~A_(14) random primer들이 토마토에 있어 적합한 것으로 판단되었으며 45 cycle의 반응횟수가 명확한 다형화 band 형성에 필요한 충분량의 DNA를 확보하기에 적합한것으로 나타났고 38℃의 annealing 온도 가 공시된 토마토의 PCR에 적합한것임을 보였다. 반응 요인들의 농도와 양에서 나타난 결과로 보아 그들의 효과에 있어 각각은 매우 상호의존적인 관계에 있는것으로 판단되었다. A series of experiments were initiated to examine factors affecting Polymerase Chain Reaction(PCR) in studying Random Amplified Polymorphic DNA(RAPD) of tomato. The results showed that optimum concentrations or amounts of reaction parameters were composed of 25ng of template DNA, 5pM of 10-mer primer, 0.05mM of dNTP, and 0.25 units of Taq DNA Polymerase in 50㎕ of the final reaction mixture. In this study, random primer A_(9)∼A_(14)(Operon) were selected as proper primers for tomatoes. 45 cycles of PCR and the annealing temperature of 38℃ were proper to get enough amount of amplified DNA for clear polymorphic band formation. The results also indicated that the concentrations or amounts of reaction parameters were highly correlated with each other in their effects on DNA amplification.

스피드 스케이팅 선수들의 외상 후 성장 과정 탐색

최근원,최나영,김민정 한국레저사이언스학회 2022 한국레저사이언스학회지 Vol.13 No.1

This study investigates the psychological change of speed skating athletes who experienced the social disaster called COVID 19 Pandemic through phenomenology method. Researchers in the study targeted 5 athletes who met the conditions by using standard sampling among purposeful sampling method. Data were collected through in-depth interviews, participatory observation and on-site records and collected data were analyzed according to the four steps of Giorgi’s technical phenomenology method. For the veracity of the study, a review was conducted by researchers and conducted triangulation method during the process of reviewing the data. As a result of data analysis, 50 sense group, 15 sub-components were drawn, and situational structure techniques of psychological changes were derived through the process of change, difficulty, adaption and overcome. Elite speed skating athletes went through prolonged negative psychologies such as anxiety, dissatisfaction, loss, and loss of sense of purpose from trauma caused by COVID-19, but it was confirmed that they had derived post-traumatic growth from intrusive rumination to deliberate rumination.

인스타그램 인플루언서의 활동은 놀이인가, 노동인가?: 인스타그램 마이크로 인플루언서를 중심으로

최근원 강원대학교 사회통합연구센터 2022 사회통합연구 Vol.3 No.2

본 연구는 2030세대인 MZ세대 사이에서 현재 굉장히 영향력 있는 인스타그램 마이크로 인플루언서들의 활동이 놀이인지 노동인지에 대해 중점적으로 살펴보았다. 이에 현재 인스타그램 마이크로 인플루언서로 활동하고 있는 7명의 인플루언서들을 1대1 반구조화된 심층 인터뷰를 진행하였다. 또한, 이들이 마이크로 인플루언서 활동을 지속하기 위해 어떠한 노력을 하고 있으며, 마이크로 인플루언서 활동을 하며 어떠한 스트레스를 받고 있는지도 함께 알아보았다. 이를 통해 다음과 같이 세 가지 결론을 도출했다. 첫째로 마이크로 인플루언서들의 활동은 분명히 노동의 성격을 포함하고 있다. 둘째로 인스타그램 마이크로 인플루언서들 내에서도 팔로워 숫자와 영향력에 따라 활동을 인식하는 정도와 유지하기 위한 노력의 차이가 존재한다. 마지막 셋째로 마이크로 인플루언서들은 불순한 의도 혹은 스팸성 DM으로 인해 스트레스를 받고 있다. 즉, 마이크로 인플루언서의 활동은 놀이보다는 노동에 가까우며, 적지 않은 스트레스를 받고 있는 것으로 나타났다.

고추 PCR 조건의 최적화

최근원,이승우,남승현,설일환,유일웅 경희대학교 부설 식량자원개발연구소 1996 硏究論文集 Vol.17 No.-

신품종에 대한 육종가의 권리보호용 고추 RAPD 마커를 개발하기 위한 기초연구로 PCR에 영향을 주는 요인들에 대한 최적화가 이루어졌으며 주요 결과를 요약하면 다음가 같다. 1. MgCl₂ 농도는 DNA증폭에 직접적인 영향을 주었으며 본 실험의 결과는 고추 PCR에 요구되는 MgCl₂의 최소농도는 3mM임을 보였다. 2. 공시된 2고추 조합간데 차이는 있었지만 Taq DNA polymerase는 1.5U이상의 조건일 때 DNA증폭에 효과적임이 관측되었다. 3. 본 실험에 사용된 template DNA양 중 최소인 10ng의 수준으로도 성공적인 DNA증폭을 보였다 4. dNTPs농도는 200~400μM 수준이 적당하였다. 5. Primer의 농도가 높아짐에 따라 band의 선명도가 증가하는 경향을 보였으며 200nM이상의 농도에서 뚜렷한 band가 관측되었다. 6. 공시된 두조합에서 모두 annealing 온도가 높아짐에 따라 minor band의 수가 줄어들고 major band의 선명도가 증가했다. 7. 본 실험의 결과는 3mM의 MgCl₂, 1.5U의 Taq, DNA polymerase, 10ng의 template DNA, 200μM의 dNTPs, 200nM의 primer, 그리고 42℃의 annealing 온도가 고추의 random primer를 이용한 PCR이 적정수준이 될 수 있음을 제시하였다. Factors affecting PCR have been optimized as a fundamental study in developing RAPD markers to protect breeder's right for new cultivars in Capsicums. DNA amplification was directly influenced by MgCl₂ concentration and the result showed that the minimum concentration required for PCR in Capsicums was 3mM. Although there was a difference between two pepper combinations tested, it was observed that over 1.5U of Taq. DNA polymerase was effective in DNA amplification. A successful DNA amplification was obtained with 10ng of template DNA, the smallest amount tested in this experiment. The proper level of dNTPs was the range between 200 and 400μM. There was a tendency that the intensity of bands was increased as the concentration of random primer increased and clear bands were observed with the concentration above 200nM. In both pepper combinations tested, the number of nonspecific bands was decreased and the intensity of major bands was increased as the annealing temperature was increased. The results suggested that the optimum conditions for PCR with random primer in Capsicums could be obtained with 3mM of MgCl₂, 1.5U of Taq. DNA polymerase, 10ng of template DNA, 200μM of dNTPs, 200nM of random primer, and 42℃ of annealing temperature.

生育初期의 溫度가 피만고추의 開花 및 着果에 미치는 影響

崔根元 경희대학교 부설 식량자원개발연구소 1992 硏究論文集 Vol.13 No.-

Seedlings of 'New Ace' and 'Lady Bell' pepper (Capsicum annuum) were exposed to low, medium, high tempertures for 24 days after transplanting into pots to study the influence of temperature at the early growth stage in flowering and fruiting. Pepper plants pretreated with the high temperature of 30/15℃ (day/night) showed a significant increase in the leaf and branching point number, and this resulted in the formation of more flowers and fruits. During the pretreatment, a significant number of flowers on the first branching point were failed to grow under the high temperature condition because of the abscission layer formed within the peduncle and the abnormal flower bud development.

Agrobacterium을 이용한 고추의 형질전환체계개발

최근원,박영두,박승국,전영주,유일웅,김인태,백운돈 경희대학교 생명자원과학연구원 1997 硏究論文集 Vol.18 No.-

본 실험은 고추의 형질전환체 선발을 위한 최소의 항생제농도를 결정하고 그 결과를 이용하여 형질전환체를 유기하고 효율을 최대화하기 위하여 수행한 바 그 결과는 다음과 같다. 1. 항생제 감응성 검정결과 kanamycin 40㎎/ℓ와 hygromycin 20 ㎎/ℓ에서 신초형성이 완전히 억제되었다. 2. Agrobacterium 제거에 사용되는 carbenicillin과 cefotaxime 처리시 재분화에 아무런 영향을 미치지 않았으며 cefotaxime 200㎎/ℓ 처리에서는 무처리구보다 신초형성이 더 활발하였다. 3. 회복친인 135Q와 276F에서는 형질 전환시 높은 재분화율을 보였으나 형질전환율은 각각 10%와 11.1%의 낮은 빈도를 보였다. 4. 재분화된 신초들을 대상으로 histochemical GUS assay를 실시한 결과 부분적으로 푸른발색이 확인되었다. 5. GUS활성을 보인 신초들에 대한 NPTⅡprimer를 이용한 PCR검정에서 NPTⅡ 유전자의 존재가 확인되었다. A transformation system using Agrobacterium tumefaciens was developed with different genotypes of hot peppers (Capsicum annuum). Four antibiotics commonly used in Agrobacterium-mediated transformation were tested for their effects on shoot regeneration. Shoot induction was completely inhibited by kanamycin at 40㎎/ℓ and hygromycin at 20㎎/ℓ or higher, but not by carbenicillin and cefotaxime. Cotyledon explants of hot peppers were cocultivated with Agrobacterium turnefaciens LBA4404 which carries a binary. vector pBI121 harboring the neomycin phosphotransferase, type Ⅱ (NPT Ⅱ) gene and β-glucuronidase (GUS) gene. For selection of transformed cells, 40㎎/ℓ kanamycin were used because the sensitivity test showed that 40㎎/ℓ kanamycin completely inhibited shoot induction from nontransformed cells. Histochemical GUS assay showed the presence of the GUS gene and PCR analysis confirmed the NP TⅡ gene was integrated. Transformation frequency was about 10.0% and 11.1% of total regenerants from 135Q and 276F, respectively.

Plant Regeneration from Cotyledon Explants in Leaf Chicory (Cichorium intybus L. var. foliosum)

최근원,김대성,황현정,채원병,이윤형 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1

Plantlets of leaf chicory ‘Radicchio’ and ‘Italiana’ (Cichorium intybus L. var. foliosum) were produced through organogenesis from cotyledon explants cultured on MS basal medium supplemented with various concentrations of indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP) combinations. Most vigorous callus and shoot formation of ‘Radicchio’ were observed in the combination of 1.0 mg・L-1 IAA and 1.0 mg・L-1 BAP and those of ‘Italiana’ in 0.1 mg・L-1 IAA and 1.0 mg・L-1 BAP. Silver nitrate (AgNO3) which was applied to reduce vitrification was also effective on the formation of single shoots; also, 10 and 5 mg・L-1 AgNO3 were selected as the most effective levels in ‘Radicchio’ and ‘Italiana’, respectively. Root formation was promoted when regenerated shoots were cultured in 1/2 MS medium with 0.5 mg・L-1 naphthaleneacetic acid (NAA) for both ‘Radicchio’ and ‘Italiana’ although adventitious roots were formed without NAA. Rooted plants were transferred into pots and thereafter, acclimatized and maintained in proper greenhouse conditions.

Salvia microphylla 에탄올추출물의 항산화 및 항염에 관한 연구

최근원,조현미,최인호 한국피부과학연구원 2023 아시안뷰티화장품학술지 Vol.21 No.3

Purpose: This study was aimed at investigating the anti-inflammatory and antioxidant properties of Salvia microphylla (SM) extract (SME). Methods: Powdered SM was treated with ethanol and decocted to acquire SME. The antioxidant activities of SME were assessed by evaluating its total polyphenolic, and flavonoid , contents and performing 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. The antiinflammatory activities of SME were verified by examining the inhibition of nitric oxide (NO) production, and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS) activated RAW 264.7 macrophages. Results: The total polyphenol and flavonoid contents in SME were 102.26±0.39 mg GAE/g and 377.42±0.77 mg QUE/g, respectively. The DPPH and ABTS assays revealed the antioxidant activities of SME by exhibiting their ability to scavenge radicals in a concentration-dependent manner (50-1000 μg/mL). The cell viability of SME exceeded 99% at 50-1000 μg/mL. The concentration-dependent reduction in the expression of the inflammation mediator iNOS and subsequent NO production indicated an approximately 100% inhibition efficacy at 600 μg/mL (0.00±1.34%). These results confirmed the potential of SME as a component of cosmeceuticals. Conclusion: Based on the findings of this study, SME is explicitly acknowledged for its applicability as a natural preservative possessing antioxidant and antiinflammatory activities as well as a raw material for cosmeceuticals intended to alleviate skin irritation.

지방 꽃 피다 : 명품 고객서비스, 세계1등 도시 -서울특별시 서초구

최근원 대한지방행정공제회 2011 地方行政 Vol.60 No.697

생장조절제 및 항생제가 고추의 기내 기관형성에 미치는 영향

최근원,박영두,유일웅,설일환 慶熙大學校 1997 論文集 Vol.26 No.-

An efficient organogenesis system was developed from in vitro cotyledon tissues of 4 pepper cultivars. The vest medium was Murashige and Skoog medium with 0.05 mg/L NAA and 3.0 mg/L zeatin. Four antibotics commonly used in Agrobacterium-mediated transformation were tested for their effects on shoot regeneration. Shoot induction was completely inhibited by danamycin at 40 mg/L and hygromycin 20 mg/L or higher, but not by carbenicillin and cefotaxime.