Complete genome sequence and comparative analysis of Streptomyces seoulensis, a pioneer strain of nickel superoxide dismutase

Jihoon Shin,Shinae Park,Jung‑Shin Lee,Eun‑Jin Lee,Hong‑Duk Youn 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Streptomyces seoulensis has contributed to the discovery and initiation of extensive research into nickel superoxide dismutase (NiSOD), a unique type of superoxide dismutase found in actinomycetes. Still so far, there is no information about whole genome sequence of this strain. Objective To investigate complete genome sequence and perform bioinformatic analyses for genomic functions related with nickel-associated genes. Methods DNA was extracted using the Wizard Genomic DNA Purification Kit then sequenced using a Pacific Biosciences SMRT cell 8Pac V3 DNA Polymerase Binding Kit P6 with the PacBiov2 RSII platform. We assembled the PacBio longreads with the HGAP3 pipeline. Results We obtained complete genome sequence of S. seoulensis, which comprises a 6,339,363 bp linear chromosome. While analyzing the genome to annotate the genomic function, we discovered the nickel-associated genes. We observed that the sodN gene encoding for NiSOD is located adjacent to the sodX gene, which encodes for the nickel-type superoxide dismutase maturation protease. In addition, several nickel-associated genes and gene clusters-nickel-responsive regulator, nickel uptake transporter, nickel–iron [NiFe]-hydrogenase and other putative genes were also detected. Strain specific genes were discovered through a comparative analysis of S. coelicolor and S. griseus. Further bioinformatic analyses revealed that this strain encodes at least 22 putative biosynthetic gene clusters, thereby implying that S. seoulensis has the potential to produce novel bioactive compounds. Conclusion We annotated the genome and determined nickel-associated genes and gene clusters and discovered biosynthetic gene clusters for secondary metabolites implying that S. seoulensis produces novel types of bioactive compounds.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

자료포락분석(DEA)를 이용한 보건의료의 효율성 측정

김남송,박홍섭,김은진,김설화,김정숙,김현아,김경숙,신지혜,최은정,양은진,유선미,유수진 圓光大學校 醫科學硏究所 2007 圓光醫科學 Vol.22 No.1

쓰레기 매립장 주변 농촌 주민들의 삶의 질 연구

이명경,최준열,김인경,조영아,김영신,정혜진,김리나,이영규,조영태,Lee, Myung-Kyung,Choi, Jun-Yeol,Kim, In-Kyoung,Cho, Yeong-Ah,Kim, Young-Shin,Jung, Hye-Jin,Kim, Li-Na,Lee, Young-Kyu,Cho, Young-Tae 대한예방의학회 2006 예방의학회지 Vol.39 No.4

Objectives: This study aims to examine if a garbage dumping site has real and negative influence on the quality of life (QOL) for the nearby residents. The net effects of the residential distance from the garbage dumping site and from the garbage truck route were investigated for five domains of the QOL. Methods: Two hundred fifty seven Shin-dong Myeon residents, Chun-cheon Si, participated in a self-administrated survey. The Shin-dong Myeon garbage dumping site began operating in 1996. ANCOV A with generalized linear models and multiple regression analysis were performed. Results: Descriptive analyses show that a residence nearby a garbage dumping site is negatively associated with the physical and environmental domains of the QOL. The residential distance from the garbage truck route does not exert any significant effect on various domains of QOL, except for the environmental domain. On the multivariate analysis, the residents living near the garbage dumping site tended to have a significantly negative QOL in the physical and environmental domains. However, the distance from the garbage truck route did not show a significant nor substantial effect on the QOL. The demographic and socioeconomic control variables are associated with a number of the QOL domains, and their patterns are consistent with the general expectations. Conclusions: The results indicated that a garbage dumping site is considered to be an environmental hazard among the nearby residents according to the lower scores on the physical and environmental domains of the QOL. The findings from this study provide comprehensive understanding on the residents' QOL, and they may help politicians and policy makers make decisions for appropriate interventions.

음성 인식 헬스케어 시스템

신진우,조우승,조성빈,김재훈,정지은,사예지,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

As modern people's interest in health grows, wearable healthcare-related technologies that can collect biometric information and receive health-related services regardless of time and place are developing. This paper proposes the healthcare system based on voice recognition function. Unlike conventional wearable healthcare systems, the proposed system provides real-time telemedicine and diagnosis and the direct connection with doctors is possible through it. And almost all functions of the application in it are operated with voice commands to lower user access barriers. Because it deals with personal information including medical information, encryption algorithms have been applied between all data movements. Finally, the healthcare system proposed in this paper is expected to increase convenience in health care and reduce the medical gap.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

정신분열병에 대한 Risperidone의 효과 및 안전성에 관하여

신석철,왕성근,지익성,이선우,이규광,이봉희,이진영,황선희,신용재,배경도,김정란 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

In order to evaluate the efficacy and safety of risperidone, 10 patients with chronic schizophrenia were examined for 8 weeks in a silgle-open study. After the wash-out period of 1 week, risperidone was administered. Efficacy was evaluated by means of Positive and Negative syndrome Scale for Schizophernia(PANSS) and Clinical Global Impression(CGI). Safety assessment included the Extrapyramidal Symptom Rating Scale(ESRS), vital signs, body weight, ECG, and laboratory tests. The results were as follows 1) On the PANSS total score, there was significant improvement of total score after 1 week (P<.05) of administratiom. 2) On the PANSS positive and negative subscal, there were significant improvement of posotive and negative scores after 2 weeks (P<.05) of administration. 3) On the PANSS general psychopatholgy subscals, there were significant improvement of general psychopathologyscores after 1 week (P<.05) of administratiom. 4) On the CGI, there was significant decreasement of clinical impression of severity of schizophrenia score after 2 week (P<.05) of administration. 5) A statistically significant increase in body weight (P<.05) was observed after 8 weeks of administration. 6) EPS reached the peak at the end of the 1st week of administration of risperidone (11.8± 24.25) but they were easily relieved by addition of benztropine and clonazepam. There was not significant change in laboratory tests, vital sign, ECG after 8 weeks of administration. These results suggest that Risperidone is an effective antipsychotics and clinically safe except for increased body weight in chronic schizophrenia.

'-ㄹ]Noun+ㅅ+Noun' 合成語의 通時 語彙論

愼重珍 국어학회 2004 국어학 Vol.0 No.44

오늘날의 '칼날, 글귀, 길가, 물결, 물고기, 밀가루, 발톱, 솔방울, 활시위, 뭇사람, 이튿날, 사흗날, 일기, 바닷가, 바닷물, 송진, 햇빛'과 같은 어휘는 각각 중세국어 시기의 '?Fㄴㆍㄹㅎ, ?G句, ?zㄱㆍㅅ, ?逵? ?逵慈? ?Bㄱㆍㄹㆍ, ?M톱, ??방올, ?시울, ?O사ㄹㆍㅁ, 이?날, 사ㅇㆍㄽ날, ??氣運, 바ㄹㆍㄽㄱㆍㅅ, 바ㄹㆍㄽ믈, ??진, ??빛' 등에 대응되는데, 모두 '-ㄹ]Noun+ㅅ+Noun'의 동일한 內的 構造를 갖는다. 본고에서는 오늘날 상이한 구조의 어휘들이지만, 기원적으로는 동일한 구조의 어휘들을 相同構造의 어휘라고 부른다. 결과적으로 字素形을 중심으로 볼 때, '-ㄹ]Noun+ㅅ+Noun'이었던 어형들은 크게 세 가지 유형으로 심리 어휘부(mental lexicon)에 전승·저장되었는데, 'ㄹ' 유지형인 '칼날, 글구/글귀, 길가, 물결, 물고기, 밀가루, 발톱, 솔방울, 활시위' 등, 'ㄹ' 탈락형인 '뭇사람; 이튿날, 사흗날' 등, 그리고 대체형인 '일기(日氣), 바닷가, 바닷물, 송진(松津), 햇빛' 등이 그것이다. 즉 이들은 원래 상동구조였으나 전승·저장되는 과정에서 심리 어휘부 속의 어근 어휘부나 접사 어휘부의 각기 다른 간섭의 결과로 서로 상이한 내적 구조 유형에 속하게 된 것이다. 본고에서는 현대국어 시기의 어형을 바탕으로 나눈 이 세 가지 유형에 따라 어근 어휘부와 접사 어휘부가 이들의 변화에 각각 어떻게 간섭하였는지를 밝힌다. This paper aims to take a close look at '-ㄹ]Noun+ㅅ+Noun' compounds that appered upon the scene of history variously. For example, '?Fㄴㆍㄹㅎ, ?G句, ?zㄱㆍㅅ, ?逵? ?逵慈? ?Bㄱㆍㄹㆍ, ?M톱, ??방올, ?시울, ?O사ㄹㆍㅁ, 이?날, 사ㅇㆍㄽ날, ??氣運, 바ㄹㆍㄽㄱㆍㅅ, 바ㄹㆍㄽ믈, ??진, ??빛'. As far as '-ㄹ]Noun+ㅅ+Noun' compounds is concerned, we denominate lexemes of the etymologically same structure lexemes of "the homologous structure(相同構造)". From the standpoint of a grapheme, '-ㄹ]Noun+ㅅ+Noun' compounds are divided into three groups, which have been transmitted to the mental lexicon. For example, '칼날(the blade of a knife), 글구/글귀(passage), 길가(roadside), '물결(wave), 물고기(fish), 밀가루(wheat flour), 발톱(toenail), 솔방울(pine cone), 활시위(bowstring)' etc belong to 'ㄹ' maintenance group, '뭇사람(crowd); 이튿날(next day), 사흗날(third day)' etc belong to 'ㄹ' omission group, and '일기(weather), 바닷가(beach), 바닷물(sea water), 송진(pine resin), 햇빛(sunshine)' etc belong to alternation group. Because the root lexicon and the affix lexicon of the mental lexicon have interfered in '-ㄹ]Noun+ㅅ+Noun' compounds. Accordingly they have been belonged to the unlike internal structures.

수요자의 요구와 아파트 단위세대 평면계획의 상관관계에 관한 연구 : 용인지역을 대상으로

신중진,박성진,김명훈,김태엽 대한건축학회 2004 대한건축학회논문집 Vol.20 No.2

The purpose of this study is to define the correlation between consumer's needs appearing diversely in recent years and characteristics of apartment unit plan derived from categorizing composition forms of unit plan. The results of this study are as follows; ① The separation and connection of public zone have relation to use space economically with living room in the center. ② The opened space composition is reflected to an increase in size and openness of the living room. ③ The variability by the hierarchy change of space appears into the introduction of variable designs through light wall and family room. ④ The protection of privacy and communication have relation to the separation of master zone and conversion of inner room to family communication space. ⑤ The diversity and individualization of rooms appear into giving a special function to rooms increased by the extension of bay. ⑥ the extension and connection of service space are reflected to an increase in size of balcony and storage and the flexibility of utility space. ⑦ The recovery of emotionality has relation to the introduction of interior garden.

부산 서구 저탄소 녹색도시사업을 통한 지역환경 개선 및 활성화 방안 연구

정숙진, 윤성환, 우신구 신라대학교 부산학연구센터 2012 부산연구 Vol.10 No.-

전 세계의 환경문제인 기후변화에 적극적으로 대응하기 위해 새로운 국가 비전인 저탄소 녹색도시을 견인할 수 있는 지역·권역별 녹색도시화 특성화 방안을 제시함으로서 기후변화에 대응할 수 있는 방안을 모색하고자 한다. 부산 원도심인 서구 충무동·남부민동의 풍부한 자연자원과 다양한 역사·문화자원을 활용한 저탄소 녹색성장과 환경보호를 양립시켜 새로운 성장동력과 지역경제 활성화로 도시재생방안을 마련한다. 녹색기술을 활용한 경사지 주거재생, 도심형 항만재생, 원도심 상권재생의 3대 목표를 설정하고 6대 핵심 전략을 중심으로 지속가능한 녹색도시를 조성한다. 서구 저탄소 녹색도시 선도사업에서 노후화된 주거시설을 개선하기 위한 하우스 프로젝트을 통해 지역성에 맞는 주거모델을 제시하고 친환경 기술요소를 이용하여 자체에너지 생산, 그린 인프라를 이용하여 생태복원 및 바람길을 연결하여 미기후 조절을 가능하게 한다. 그리고 도심형 항만재생에서는 공동어시장에 발생하는 유기성폐기물을 바이오매스시스템 구축을 통하여 바이오에너지를 생산하며 해양친수공간 조성과 천마산을 연결하는 그린네트워크를 형성하여 부산의 대표적인 저탄소 녹색성장 명품도시로 조성한다. By suggesting the green city characterization measure for each region and zone that can lead the low carbon green city which is a new national vision in order to actively cope with the climate change, the global environment issue, we aim to seek for the measure to build up low carbon green city suitable for local characteristics. With low carbon green growth and environment protection by using abundant natural resources and various historical/cultural resources in Seo-gu Chungmu-dong and Nambumin-dong, the old downtown of Busan, the city reclamation measure can be prepared based on a new growth driving force and local economic vitalization. It is a general measure related to easing and adapting to climate change like water circulation, including setting up the three main goals like reclamation of residence on slopes, urban ports and business districts in old downtown based on green technology, and the six core strategies like establishing system of carbon reduction through using low carbon energy, improving the transportation structure through introducing green traffic system, having water/resource circulation system through low carbon circulation resource, having green construction system through applying green technology and environmentfriendly materials, building up ecotype greens based on the carbon absorption system through management of greens and urban forest and creating green community through private and public green partnership. It is possible to control microclimate by connecting wind ways, restoring ecology through using green infrastructure and producing its own energy based on environment-friendly technical elements as also suggesting residental models suitable for local characteristics based on the house project to improve obsolete residential facilities with low carbon green city leading project in Seo-gu. For the urban port reclamation, bio-energy is produced by building up biomass system through organic waste generated in the joint fish market. As also forming waterfront and green network connecting with Mt. Cheonma, it can be the representative low carbon green growth city in Busan.