Combined Gene Therapy with Hypoxia-Inducible Factor-1α and Heme Oxygenase-1 for Therapeutic Angiogenesis

Bhang, Suk Ho,Kim, Ju Hee,Yang, Hee Seok,La, Wan-Geun,Lee, Tae-Jin,Kim, Ga Hee,Kim, Hyun Ah,Lee, Minhyung,Kim, Byung-Soo Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.7

<P>Transfection with either hypoxia-inducible factor-1α (HIF-1α) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1α gene occurs rapidly, the expressed HIF-1α protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1α and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1α or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1α and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1α gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1α and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.</P>

키넥트 센서를 이용한 고령자 대상의 선자세 균형능력 평가

양승태(Seung-Tae Yang),강동원(Dong-Won Kang),서정우(Jeong-Woo Seo),김대혁(Dae-Hyeok Kim),김태호(Tae-Ho Kim),최진승(Jin-Seung Choi),탁계래(Gye-Rae Tack) 대한전기학회 2017 전기학회논문지 Vol.66 No.2

Portable low-cost Kinect sensor was used to analyze standing balance ability of the elderly. Eighty subjects who can walk alone and have a normal cognitive level participated in this experiment. Based on Berg Balance scale (BBS) test with 52 points, subjects were divided into Healthy older (HO: 46 persons, BBS: 53.80 ± 1.19) and Impaired older (IO: 34 persons, BBS: 49.06 ± 2.03) group. Each subject performed 30 seconds four different standing balance tests (EO: Eyes Open, EC: Eyes Close, EOf: Eyes Open on foam, ECf: Eyes Close on foam). Five variables (Mean distance, Range of distance, Root mean square, Mean velocity, 95% ellipse area) were calculated from the hip joint center movement of Kinect sensor. Results showed that there were significant differences between groups for four different standing tests. Calculated variables from kinect sensor showed significant correlation with BBS score. Especially, mediolateral mean distance, mediolateral root mean square, mediolateral range of distance and 95% ellipse area showed discriminative ability for all tests. Mean values of variables of IO were higher than those of HO, which means the decreased balance ability in IO compared with HO. Therefore, it was possible to estimate simple balance assessment of the elderly using portable low-cost Kinect sensor.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

과학 영재 프로그램을 통해 본 과학 관련 태도와 과학 불안도의 관계와 변화

양태연,박상우,박인호,한기순 한국과학교육학회 2005 한국과학교육학회지 Vol.25 No.2

본 연구는 과학 영재 학생의 과학 관련 태도와 과학 불안도와의 관계가 어떠한 상관을 보이고 있고, 과학 관련 태도와 과학 불안도가 과학 영재 프로그램에 참여하기 전과 참여한 후에 어떻게 변화하는지를 보았다. 이에 따라 과학 영재 교육 프로그램에 참여하고 있는 148명의 중학생들을(남학생 : 95명, 여학생 53명)대상으로 과학 관련 태도 검사와 과학 불안도 검사를 실시하였다. 연구결과, 과학 관련 태도와 과학 불안도의 관계는 사전 검사에서 뚜렷한 부적 상관을 보였으나 사후 검사에서는 부분적으로 정적 상관이 나타났고 영재 프로그램 참여 후 과학 영재들의 과학 관련 태도는 낮아졌고 과학 불안도는 증가하였다. 이 연구의 흥미로운 점은, 여러 선행 연구결과와는 달리 영재 프로그램에 참여한 후 남학생이 여학생보다 과학 관련 태도가 낮아졌고 과학 불안도가 증가하였다는 점이다. This study examined scientifically gifted students' science attitude, anxiety, and their relationship. In addition, sex differences in these variables were investigated. The main interest of the study, however, was to explore how scientifically gifted students' science attitude and anxiety have changed before and after a gifted education experience. One hundred and forty-eight middle school students (95 boys and 53 girls) who enrolled in a gifted education program participated in the present study. The results indicate an increase in science anxiety and a decrease in science attitude after the six-month gifted program experience. Negative correlations between science attitude and anxiety were found in pre-test, but some positive relationship existed in post-test. Interestingly, after the specialized gifted program, male students' science attitudes were lowered significantly. In contrast, there was no statistically significant difference in female students. Results of the study were discussed in relation to the programming issues for the scientifically gifted.

大學校 敎職員의 健康實態에 관한 基礎的 調査硏究

양점홍,황철문,남태호,백영호,윤양진,황영성 부산대학교 사범대학 1993 교사교육연구 Vol.26 No.-

The purpose of this study was to analyze the staffs' health of P. University through survey research. Also, the staffs's health problem was analyzed by the regular health test's result and their lifestyle. The results were as following: 1. The analysis of lifestyle (1) A habit of smoking 1. It was presented the male smokers(55.9%) and females smokers(0.6%) of P. university staffs were found in this study. 2. It was presented the male smokers smoked average 18.2 pieces a day. Also, they began to smoke cigarette 21.5 years old. 3. The persistent smokers were 24.6% and 52.2% of smokers be going to quit smoking cigarette someday in the future. (2) A habit of drinking 1. It was presented 85.7% of males and 59.1% of females subject drinking normally. 2. It was presented 3.7% of male subjects were drinking alcohol almost everyday in a week. 3. The most frequent kind of alcohol is as following order a beer(males 67.6% & females 79.6%) and a soju(males 28.3% & females 4.3%). Males drunken average amount of beer was 2 bottles(640ml) and females drunken average amount of beer was 0.8 bottles in a week. (3) A habit of exercise 1. It was presented males (29.0%) and females (15.1%) were exercising regularly, but males (23.3%) and females (38.6%) of staffs were not exercise at all. 2. It was found males' regular kind of exercise was as following order mountain-climbing, tennis, and jogging and females' regular kind of exercise was as order swimming, free handing exercise, and mountain-climbing. 3. It was presented 25.1% of staffs exercised 6 times a week regularly. And each times of exercise, 37.3% of staffs spent less than 30 minutes. 4. It was presented staffs exercised for health (72.4%) and 48.4% of staffs could not exercise as following reasons of not enough times, home work, research.... 5. Male staffs prefer as following order kind of sport tennis, swimming, and golf and female staffs prefer as following order kind of sport swimming, tennis, and bowling in the future. (4) A habit of eating 1. It was presented 5.5% of males and 16.6% of females did not have a breakfast regularly. 2. Concerning balance of food content: do not concerning food (males 34.7% & females 23.8%) and subjects like vegetables 9males 25.7% & females 32.7%) were found. 3. It was presented 8.5% of males and 5.4% of females took the vitamin regularly. (5) Health condition 1. In present evaluation for subject's health condition, very healthy (males 7.5% & females 6.0%) and poor health (males 2.0% & females 1.8%) were found in this study. 2. In present evaluation criterion for subject's health condition, the ability of adaptation on fatigue was appeared the most (males 35.9% & females 40.7%). 3. It was presented subjects have a digestive trouble (males 23.5% & females 33.1%) which were the best health problem in subjects. 2. A analysis of result of regular health test (1) Physique test 1. It was presented height (males 169.5cm & females 158.6cm) and body weight (males 65.9kg & females 53.1kg) were found in this study. 2. According to this study, obesity (males 11.2% & females 5.6%) and low body weight (males 8.8% & females 14.8%) were found in this study. 3. A high blood pressure subject (males 8.7% & females 2.5%) over than a contraction blood pressure 140mmHg and A high blood pressure subject (males 8.5% & females 1.9%) over than a expansion blood pressure 90mmHg were found in this study. (2) A blood test 1. Normal hemoglobin of subject (males 99.8% & females 62.3%) and 37.7% of females less than normal hemoglobin were found in this study. 2. 5.3% of males and 2.5% of females have greater than a concentration of glucose 140mg/dl, but there was anyone less than a concentration of glucose 50mg/dl. 3. The greater than 251mg/dl staff''s T.C.(males 7.1% & females 4.9%) and the less than 130mg/dl staffs' T.C.(males 2.0% & females 3.7%) were found in this study. 4. It was found males 5.3%, 11.6% & females 1.9%, 4.3% were presented greater than GOT and GPT 341U/L. (3) Hepatitis, Urinary Sugar, and Urinary Protein test 1. According to this study result, positive hepatitis was presented males (6.5%) and female(4.3%). 2. Urinary sugar is greater than +- males (1.8%) and females (2.4%) were found in this study. 3. Urinary protein is greater than +- males (1.6%) and females (1.8%) were found in this study. (4) Chest X-ray test 1. It was found male (1.6%) suffered from tuberculosis of the lungs. (5) Dental test 1. It was presented 77.3% of males and 88.9% of females was not existed the upper caries. Also, 75.7% of males and 90.7% of females was not existed the bottom caries. 2. It was presented 96.8% of males and 99.4% of females had the non-deficit of teeth in the upper part of teeth. Also, 93.9% of males and 98.1% of females had the non-deficit of teeth in the bottom part of teeth. 3. It was presented 19% of males and 3.7% of females have the periodontal disease. (6) General report and judgment 1. In general report, it was found 75.3% of males and 91.4% of females were the normal health condition. It was presented 9.1% of males and 4.9% of females were the liver disease, 4.9% of males and 1.2% of females were the sugar diabetes, 4.9% of males and 0.6% of females were the high blood pressure, and 1.4% of males and 1.9% of females were high arrest of bleeding. 2. In general judgment, it was presented 75.5% of males and 91.4% of females were the normal health condition. However, it was presented 17.8% of males and 7.4% of females were required high observation, 6.7% of males and 1.2% of females were required simple observation. As presented P. University staffs' lifestyle, it was found still many staffs have not right living habit. It is necessary to be wellness of lifestyle and satisfaction of life through correction of self responsibility and management in their life. Therefore, it is possible to live with healthy in better quality of life which is high quality of life and new concept of health were offered. Also, researcher expected the regular health examination be more practical contents than informal contents. Researcher recommended that it is necessary to support the university administration to be more flexibility of the regular health examination day than set the certain day of regular health examination and it required to create a way of using the P. university hospital.

조선시대 감로탱화 풍속장면의 복식 연구

양숙향,이태호,이경화 한국의류산업학회 2003 한국의류산업학회지 Vol.5 No.5

The Gamrotenghwa of the Choson Dynasty is a unique genre of buddhist painting in that the destiny of the dead souls and the guidance to nirvana are expressed in reality. More than 50 of the Gamrotenghwa are known to he present, and the lower part of the paintings includes a wide assortment of folk customary scenes reflecting the social life style of the time when the painting was drawn, such as difficulties in life, public life, punishment and war. Changes in the costume of the people shown on the customary scenes of the Gamrotenghwa according to the time based changes in painting style were investigated in this study. The results are summarized in three points. First, the costumes of bureaucrats showed a tendency of preservation without any major changes in the painting. On the other hand, costumes of public and entertainer in the scenes were very close to those worn by the people at that time. The realistic description of public life in the painting may establish the value of the Gamrotenghwa as the historical documents. Second, the costume of the people in the painting showed a dual structure as the social positions; bureaucrats wore various official hats, large coats and belts to expose their social prestige, while the general public wore simple and convenient clothing which is divided into shirt and pants. The dual structure of the costume in the painting is in well accordance with that of the later period of Choson dynasty, suggesting that the customary scenes in the painting represent the social life style of the period. Finally, the customary scenes in the Gamrotenghwa are very variegated, which shows a variety of beauty of wearing even though they look coarse in a sense. The diversity of wearing beauty in the picture may contribute to the recreation of the beauty of shape in the new design of Hanbok.

師範系 體育敎育科 志望生의 一般運動能力檢査 基準値 設定에 關한 硏究

梁點紅,高基俊,黃喆文,金泰運,金重雄,白永鎬,表內淑,黃太相,徐國雄 釜山大學校 附設 體育科學硏究所 1989 體育科學硏究所 論文集 Vol.5 No.-

This study was analyzed the validity and the reliability of evaluation norm. which was used in choosing and grading events of the General Motor Ability Test. The General Motor Ability Test has been done for applicants for entrance to the department of physical education, college of education, Pusan National University. The subjects of this study were 433 applicants(men :387, women :46) for three years. They were tested in seven events : 1) 100m Dash, 2) 1500m Run(man), 800m Run (woman), 3) Throwing Hand-Ball, 4) Broad Jump, 5) Chinning(man), Flexed-Arm Hang(woman), 6) Zig-Zag Run, 7) Trunk Flexion. The results could be summerized as follows ; 1. In 100m Dash, 1500m Run, and Throwing Hand-Ball, man's average records were shown normal distribution which was inclined toward low ll grade. Therefore, the interval size of those three events must be regulated to lower. 2. Average records of Broad Jump in man were distributed into high (1) grade. So, the interval size must be adjusted to higher. 3. In Chinning, Zig-Zag Run, ad Trunk Flexion, man's average records were shawn normal distribution, and the interval size was similar to present records. 4. For woman, it was impossible to analyze correctly in all seven events because of the small number of subjects. So, it was for the sake of the reference. 5. The record of applicants for three years were operated to the variable quantity analysis. And the restults were these ; 100m Dash was F=3.0865(p<0.05), Chinning F=3.9309(p<0.05), Broad Jump F=14.7338(p<0.001), Throwing Hand-Ball F=4.9716(p<0.01), and Zig-Zag Run F=6.8297(p<0.001). Then, it was shown statistical meaningful difference in three groups. 6. In investigating the validity in choosing seven events, it was concluded that the events have been chosen reasonably. Because all events except for 100m Dash, and Zig-Zag Run in man were independent. From the analysis of the General Motor Ability Test, we could realized the necessity of regulating the standard and the interval size in several events. Then, we proposed the new evaluation norm which was suitable to present situation.

발진기 위상잡음 감소방안에 관한 연구

양승인,이태호 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

In this paper, the proposed oscillator structure employs both the low-frequency feedback system and the amplitede-phase control system. By means of hybrid structure, the proposed system greatly eliminates phase noise. We have designed 6.4㎓ oscillator and proposed system. Simulated results show that the phase noise is 91.88dBc/㎐ at the 10㎑ offset frequency, from these results we have found out that reduction method is very effective. The phase noise of the proposed system using a hybrid structure is quite comparable to that of conventional oscillator system. Throughout computer simulations, the proposed system shows the most enhanced performance than others.

제주 곽지유적에서 출토된 말 뼈의 골학적 관찰

양기천,신태균,김승호 제주대학교 1993 논문집 Vol.37 No.-

Equine bones including one phalanx 3 and three teeth excavated from Kwakji archaeological site, were morphologically examined, Compared with the Cheju pony and the Thoroughbred horse, the significance of the observations were tentatively evaluated. It was suggestied that the larger-limbed horses than the present Cheju pony inhabited in ancient Cheju island.

Congenital Insensitivity to Pain with Anhidrosis 환아의 치험례

양규호,최남기,김태근 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.1

본 병원에 동통을 느끼지 못하는 것으로 인한 혀나 손가락 깨물기 등이 자가 손상,무한증,반복적 발열등의 임상증상을 보이며 유전성이 없이 CIPA로 내원한 환아에서 보호자에게 세심한 보호와 우발사고 방지에 대한 교육이 무엇보다도 중요하며, 치면세마,불소도포,소와열구전색등의 예방적인 처치와 주기적으로 내원시켜 치과적인 문제를 조기에 해결하는 것이 필요하다고 사료된다. Congenital Insensivity to Pain with Anhidrosis(CIPA) is rare autosomal recessive disorder which is known to be hereditary sensory and autonomic neuropathies(HSAN) typeⅣ. It is predilection for male and observed in early infant. Its clinical features include congenital analgesia, which leads to self-mutilation; inability to sweat, which leads to defective thermoregulation; and mild to moderate mental retardation. Its dental features include self-mutilation by nail peeling, which leads to gingival ulcer, tongue ulcer due to tongue biting, and enamel hypoplasia. Partial anodontia is often observed as well. Seventeen-months-old boy with CIPA was reported. This is the case in which the dental characteristics are described and the dental treatment of patient is discussed.