김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

Hydroa Vacciniforme의 1예

이종석,김영근,김홍직,현경준 順天鄕大學 1989 순천향대학논문집 Vol.12 No.3

급성심근경색증 환자에서 발생초기의 지단백질 변화

이혜진,신길자,조홍근,박시훈 梨花女子大學校 醫科大學 醫科學硏究所 2000 EMJ (Ewha medical journal) Vol.23 No.3

연구배경 : 저자등은 한국인 급성심근경색증 환자에서 첫째, 급성 흉통이후 발병초기에서 지질치의 변화를 관찰하고 둘째, 기존의 고지혈증 유무와 성별등이 지질 변동치에 영향을 주는지를 규명하며 셋째, 급성흉통 이후의 어느 시기에 측정하는 것이 기저지질치와 가장 유사하여 치료의 기준으로 삼을 수 있는가를 알아보기 위하여 본 연구를 시행하였다. 방법 : 급성흉통으로 응급실을 내원한 환자중, 28명의급성 심근경색증 환자를 대상으로 응급실 내원당시와 입원 다음날 공복시 총콜레스테롤, 저밀도 콜레스테롤, 고밀도 콜레스테롤, 중성지방을 측정하여 다음과 같은 결과를 얻었다. 결과 : 1) 급성심근경색증 환자에서 총콜레스테롤, 저밀도 콜레스테롤은 의의있는 감소를 보였다. 2) 지질변동치는 응급실 내원당시 지질수치와 양의 상관관계를 보였다. 3) 남성군에서 여성군에 비해 저밀도 콜레스테롤의 더 큰 감소폭을 보였다. 결론 : 급성심근경색증으로 응급실을 내원한 환자에서 입원 다음날 측정한 콜레스테롤치는 응급실 내원당시에 비해 의의있게 감소된 수치를 보였다. 이는 내원 다음날 측정한 지질치가 고지혈증의 진단과 치료 시작에 잘못된 판단기준으로 작용할 수 있다는 점을 시사한다. 결론적으로 급성흉통으로 응급실을 내원하는 환자에서 초기 지질측정이 필수적이며, 초기에 검사가 이루어지지 못한 경우는 지질치가 기저치에 비해 낮게 측정됐음을 고려하여 고지혈증의 치료여부를 결정해야 할 것이다. 본 논문에서는 급성흉통으로 응급실을 내원한 급성 심근경색증 환자에서 내원 당시의 수치와 비교해 볼 때 내원 다음날의 총콜레스테롤은 11.5%, 저밀도 콜레스테롤은 9.5% 감소하였다. Objectives : Hyperlipidemia is an important risk factor of coronary atherosclerosis. Serum lipids, especially cholesterol level is closely related to coronary artery and early identification and treatment of hypercholesterolemia reduced the risk of ischemic heart disease. In secondary prevention studies, lipid regulation has been demonstrated to result in a reduced incidence of myocardial infarction and mortality. But during the acute phase of a myocardial infarction, the serum lipid pattern is known to be rapidly changed and consequently dose not reflect the baseline level of the patient. Total serum cholesterol concentrations measured within 24 hours after acute myocardial infarction are likely to reflect basal levels, thus they must be used as the reference for the diagnosis and treatment of hyperlipidemia. If serum lipid levels were not measured within 24 hours of acute chest pain, it is essential to correct the lipid level to the baseline level. So we investigated the following. First, serum lipid alteration during the acute phase of acute myocardial infarction, second, the factors that are related to lipid change, third, the time to check the baseline value of lipid level during the acute phase of myocardial infarction. Methods : We have measured the total cholesterol, LDL cholesterol, HDL cholesterol and triglyceride at admission time and the next day in a group of 51 acute myocardial infarction patients who had acute chest pain. Results : First, total cholesterol, LDL cholesterol at the next day were significantly reduced. Second, positive correlation was noted between lipid alteration and the lipid level that was checked at admission time. Last, male groups had more significant reduction of LDL cholesterol than female groups. Conclusion : Cholesterol levels thats were checked the next day were significantly reduced in comparison with the cholesterol value registered at hopital admission. Consequently, it is essential to check the lipid level at the time of hospital admission. But if it was not done, corrected values are a useful guide to patients basal lipid state and treatment references.

6-hydmxydopamine 파킨슨병 모델에서 Apomorphine의 투여에 따른 회전운동 변화의 시간적 추이

홍근식,김병곤,전범석,윤병우,이광우,노재규,이상복,명호진 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

실험적 적혈구 증다증에 있어서 전위효소활성의 변동 : Ⅲ. L-Ornithine Aminotransferase

홍영수,이근배 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.15 No.1

토끼에 Phenylhydrazine을 주사한 후 시일이 경과하는 동안에 채혈한 혈액의 적혈구에서 ornithine aminotransferase(L-oi-nithine : 2 oxoacid aminotransferase, EC(2.6.1.13)의 총 활성도의 변동과 세포질 및 mitochondria에 함유된 ornithine aminotransferase의 활성도의 변화를 관찰하여 다음과 같은 결과를 얻었다. ornithine aminotransferase의 활성도의 측정은 김형노 및 공동연구자의 방법에 따랐다. 정상토끼 적혈구증의 망상적혈구의 방법에 따랐다. 정상 적혈구의 ornithine aminotransferase의 활성도는 약 14.1unit/㎖ RBC였으며 세포질 분획에는 약 93.6%가 mitochondria분획에 약 2.4%가 분포되어 있었다. Phenylhydrazine후사후 5~6일이 경과하면 망상적혈구의 수는 최고치에 달하는바 약35배의 증가를 보여 준다. 적혈구내의 ornithine aminotransferase의 총 활성도도 최고치에 달하는바, 정상치의 약9.4배의 증가를 보여준다. 총 활성도의 약 97.3%가 세포질 분획에, 약2.7%가 mitochondria 분획에 증명된다. 망상적혈구증다증의 유발기에 있어서 ornithine aminotransferase의 총 활성도의 증가는 주로 세포질 분획내의 이 효소의 증가에 기인하는 것이다. (Ks=0.0339, Kd=0.0102, t1/2=72.2 hours). Mitochondria 분획내의 isozyme도 정상치의 최고 약22.1배 증가한다. (Ks=0.0339, Kd=0.0102, t1/2=67.9 hours) Phenylhydrazine 주사하였을 때 이 효소의 합성 및 분해속도 상수는 세포질 분획에 있어서는, Ks=0.4382, Kd=0.0332 t1/2=20.9 hours이며 mitochondria 분획에 있어서는, Ks=0.0060, Kd=0.0401, t1/2=17.3 hours이다. The activity of ornithine aminotransferase(L-ornithine : 2-oxoacid aminotrasnferase, EC 2. 6. 1. 13) isozymes in red blood cells were studied during induction of and recovery from phenylhy-drazine-produced reticulocytosis is from rabbit. Ornithine aminotransferase activity was assayed by the method of Kim et al. The results obtained were as follow : 1. Red blood of normal rabbit contained about 2.9% of reticulocytes. 2. Total activity of ornithine aminotransferase in normal blood was 14.1 units and the relative activity of cytosolic and mitochondrial ornithine aminotransferase isozymes were 93.6% and 2.4%, respectively. 3. The reticulocyte count in phenylhydrazine-treated rabbit increased to about 35 times on day 5. Total ornithine aminotransferase activity way maximal on day 5, about 123.4 units which is about 9.4 fold higher than normal value. The relative activity of cytosolic and mitochondrial ornithine aminotransferase isozymes were 97.3% and 2.7%. respectively. 4. The increase in total ornithine aminotransferase activity during induction of reticulocytosis was due primarily to the 9.1 fold increase in the activity cytosolic isozyrrie(Ks=1.1530; Kd=0.0096 ; t½=72.2 hours). 5. The activity of the mitochondrial isozyme increased to about 22.1 times its normal value (Ks=O.0339 ; Kd=0.0102 ; t½=67.9 hours). 6. The rate constants following recovery from phenylhydrazine treatment were; Ks=0.43 82; Kd=0.0332 ; t½=20.9 hours for mitochondrial isozyme.

반응표면 분석을 이용한 Salmonella enteritidis의 증식 효능 평가

홍정미,정효준,이홍근 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This stidy was performed to investigate the single and combined effect of concentrations of garlic juice according to the pH and temperature on the growth of Salmonella enteritdis in brain heart infusion broth, and to develope Response surface model for the effect of concentrations of garlic extract. The results of electric conductibility of Salmonella enteritidis growing in the range of incubation temperature (25~42℃), PH(5.5~9.0) and concentration of garlic juice(0~0.8%) showed that a badge with high low D.T.value and concentration of garlic extract were significantly with D.T.value and concentration of garlic extract were significantly correlated with D.T.value (p<0.01). Growth of Salmonella enteritidis in the condition of 37℃ and pH7.0 presented the lowest D.T.value.

대학생의 기본 심폐소생술 교육 평가 : 간호과와 비간호과를 대상으로

이정은,고봉연,이인모,최근명,박신일,안홍기 한국응급구조학회 2003 한국응급구조학회지 Vol.7 No.1

The purpose of this study was to evaluate the accuracy of CPR skills and the satisfaction of CPR Training targeted for college students. Also, this study made comparative study of nursing students and non-nursing students. The sample consisted of 248 students(132 nursing students, 116 non-nursing students). CPR Training was designed by two components which were a lecture and demonstration by one professor and individual practice 표 instructors. As tool of measurement estimation satisfaction of CPR Training questionnaires were developed based on educated contents. The accuracy of CPR skills were checked by SkillReporter CPR training manikin. Collected data were computerized and analyzed by SPSS-WIN program. The results were as follows : 1. The skills of CPR were cardiac compression 92.79 times/min, correct cardiac compression rate 90.85%, ventilation 9.93 times/min. correct ventilation rate 79.34%. 2. The mean of the accuracy of CPR skills were 2.94(SD .87). 3. When errors were analyzed, the highest item was stomach distension(51.2%) of ventilation skills and too little(70.6%) of cardiac compression skills. 4. The mean of the satisfaction of CPR training were 2.87(SD .17), the highest item of the satisfaction of CPR Training was practice. 5. We found significant statistical differences based on the accuracy and the error, non-nursing students were high in correct cardiac compression per minute(t=3.615, p=0...) and ventilation too much(t=4.292, p=.000), nursing students were high in correct ventilation rate(t=-3.885, p=.000) and cardiac compression too shallow) t=-2.842, p=.005).

경로당 활성화사업의 전문적 접근방안

이근홍 협성대학교 2003 협성논총 Vol.15 No.-

임대아파트의 수선비용 관리시스템을 위한 데이터베이스 구축

이춘경,정영한,박태근,김광우,김창덕,김옥규,황영삼,박홍석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

It shows that a variety of side effects result from the housing-supply policy focused in quantity. In a rental apartment for the low-income group, a shortage of maintenance cost in facilities become aggravated. The rapid progress of obsolete leads the lessor to the economic loss as well as a baneful influence to national economy. Also, the maintenance skill brings inadequacy by reason of technique development focused on new construction. As a result, the cost which needs for maintenance should get accomplished to application toward economy analysis and attempt considered management and financial affairs. Simultaneously, it should keep and administer a lot of data of maintenance affairs during life cycle in apartment and set up the way for aiding the situation of maintenance process.