四肢骨折治療後 發生된 關絶連動制限에 關한 臨床的 觀察

金世珠,吳貞姬 최신의학사 1974 最新醫學 Vol.17 No.9

Bone tissue requires a longer period to heal than any other connective tissue and immobilization with fixation during the process of heaing is absolute. Immobilization allows deposition of collagen and reticulin as a dense network instead of a loose connective tissue such as joint capsule, where movement occurs repeatedly. It will allow movement through limited distance, adapted by shortening and fixation if there is no motion, or elongate slowly under prolonged tension. The authors have observed 110 cases with extremity fractures who were treated with immobiliza?tion at department of Orthopedic surgery and refered after treatment to department of Rehabilitaion medicine from January 1972 to December 1973 and studied the relationship of immobilization with the development of limitation of range of motion in joints. The results are as follows: 1) Longer the period of immobilization developed more limited motion in joint. 2) In upper extremity;. the improvement of joint range of motion in elbow was observed steady progress with treatment regardless to the period of immobilization, but no full range of motion was retained. 3) In lower extremity, it was observed that the cases with less than 49 days immobilization in femur fracture were increased to normal functional range in knee joint with treatment but with more than 49 days immobilization not to the normal range obtained. .4) In cases with closed reduction, it was observed that better improvement noticed in joints motion than with open reduction cases. 5) 27.7% of upper extrmity fractures and 12.9% of lower extremity fractures were developed limited range of motion in non-immobilized joints.

The Genetic Studies of Obsessive-Compulsive Disorder and Its Future Directions

김세주,김찬형 연세대학교의과대학 2006 Yonsei medical journal Vol.47 No.4

Obsessive-compulsive disorder (OCD) is characterized by recurrent and persistent thoughts (obsessions), and repetitive behaviors or mental acts (compulsions). In Korea, an epidemiological study reported that the lifetime prevalence of OCD in the population was greater than two percent. The exact cause of OCD is still unknown. Evidence from familial, twin and segregation studies supports the role of a genetic component in the etiology of OCD. In addition, there is growing evidence that OCD has a specific neurochemical and neuroanatomical basis. According to this evidence, researchers have selected various candidate genes which have been implicated in the neurophysiology of OCD, and differences of allelic variants in OCD patients and controls have been analyzed. In this review we will introduce the results of previous genetic studies of OCD which have been performed in other populations, including twin studies, family studies, segregation analyses, linkage analyses, and association studies. In addition to these studies, we will present the results of our genetic studies of OCD performed in Korea.

Single-cell PCR on protargol-impregnated euplotid ciliates: a combined approach of morphological and molecular taxonomy

김세주,최중기,류성호,민기식 한국통합생물학회 2011 Animal cells and systems Vol.15 No.3

Ciliates are considered one of the most diverse protozoa and play significant roles in ecology. For successful taxonomic study of these microscopic eukaryotes, a staining procedure is necessary, due mainly to intrinsic difficulties in recognizing characteristics from living cells. Although molecular taxonomy has been used to resolve the ambiguities associated with traditional morphology-based taxonomy, extraction of genomic DNA from stained ciliate cells is not available yet. In the present study, we describe a method to extract genomic DNA from a single protargol-impregnated euplotid cell. By using HgCl_2 as a fixative and modulating the exposure time of bleach solution in the protargol impregnation, high-quality genomic DNA can successfully be extracted from a stained single cell with minimal loss of morphological integrity. This technique will contribute to the effectiveness of combined approaches of molecular and morphological taxonomy from single ciliate cells.

Taxonomic Study of Poorly-known Marine Pleurostomatid Ciliates of Litonotus paracygnus and L. pictus (Ciliophora: Pleurostomatida) from Korea

김세주,민기식 한국동물분류학회 2009 Animal Systematics, Evolution and Diversity Vol.25 No.2

Two poorly known and often confused pleurostomatid ciliates, Litonotus paracygnus Song, 1994 and L.pictus Gruber, 1884, were collected from the coastal waters of Yeonggeumjeong and Bongpo-port, Gangwondo in the East Sea and from the Iwon tide embankment near Ganwol-do, Chungcheongnam-do in the Yellow Sea, Korea. These species were described based on live observations, the protargol-impregnation and morphometrics of the species. Also provided are their diagnoses. The small subunit ribosomal DNA (SSU rDNA) sequences of these species were compared with previously known sequences of related species. The diagnostics of the two Litonotus species are as follows. L. paracygnus: 150-300 μm long in vivo, strongly contractile neck region, two ellipsoid macronuclei (Ma) and one micronucleus (Mi), 7 left (LSK) and 11-14 right somatic kineties (RSK), 2-4 contractile vacuoles (CV) located on the posterior end, extrusemes (Ex) distributed on the anterior region of the ventral margin only. L. pictus: about 200-600 μm long in vivo, extremely contractile, beautiful body color with rows of yellow to yellow-brownish cortical pigment granules, 12-21 Ma arranged in moniliform pattern, infrequently vermiform, 7-11 LSK and 18-26 RSK, several CV located on both margins, Ex distributed on the anterior region of the ventral margin only. In this study, this genus was firstly recorded in Korea.

뇌성마비아 부모의 욕구 조사

김세주,이은하,이상헌,박병규,성인영,나진경 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.6

Optimization of DNA Extraction from a Single Living Ciliate for Stable and Repetitive PCR Amplification

김세주,민기식 한국통합생물학회 2009 Animal cells and systems Vol.13 No.3

Ciliates are undoubtedly one of the most diverse protozoans that play a significant role in ecology. However, molecular examination, based on comparing the DNA sequences, has been done on a limited number of the species. Because most ciliates are uncultivable and their population sizes are often too small, it is usually difficult to obtain sufficient genomic DNA required for PCR based experiments. In the present study, we evaluated the effectiveness of four commercial DNA extraction procedures that extract high quality genomic DNA from a single ciliate cell. It was discovered that RED Extract-N-AmpTM PCR kit is the best method for removing PCR-inhibiting substances and minimizing DNA loss during purification. This method can also amplify more than 25 reactions of PCR. In addition, this technique was applied to single cells of 19 species belonged to 7 orders under 5 classes that isolated from mixed natural populations. Their small subunit ribosomal DNA (SSU rDNA) was successfully amplified. In summary, we developed a simple technique for the high-yield extraction of purified DNA from a single ciliate cell that may be more useful for rare ciliates, such as tiny and uncultivable marine microbes.

급성기 뇌졸중 환자 자살 사고

김세주,김영신,최낙경,서동향,이병철,이만홍 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

연구목적: 일반적으로 자살과 자살 사고의 가장 주된 위험 인자는 우울증, 불안장애와 같은 정신과적 질환의 동반 유무이다. 그러나 정신과적 질환 외에 신체적 질환의 동반 유무 또한 자살 및 자살 사고에 영향을 미치는 것으로 알려져 있다. 일반적인 신체 질환과 마찬가지로 뇌졸증 환자들에 있어 자살율이 증가한다는 몇몇의 보고들이 있다. 그러나 우리나라에서는 뇌졸중 환자의 자살 사고에 대한 연구가 보고된 바가 없다. 따라서 본 연구에서는 급성기 뇌졸중 환자들을 대상으로, 자살 사고의 발현율을 조사하고, 자살 사고 유무에 따른 사회문화적 변인 및 신경과적, 정신과적 임상 양상에 있어서의 차이에 대해 알아보고자 하였다. 방법: 1999년 7월부터 2000년 6월까지 한림대학교 성심병원 뇌졸중 센터에 입원한 77명의 급성기 뇌졸중 환자를 대상으로 하였다. Beck Suicidal Ideation Scale(BSIS)을 이용하여 자살 사고를 평가하였고, 우울 증상과 불안 증상은 각각 Beck Depression Inventory(BDI)와 Beck Anxiety Inventory(BAI)를 사용하였다. 사회적 지지체계의 정도는 사회적 지지체계 척도(Social Support Scale)를 사용하였으며, 뇌졸중 후 신경학적 장애의 정도는 National Institutes of Health Stroke Scale(NIHSS)와 Barthel's Index를 사용하였다. 결과: 약 35%의 뇌졸중 환자들이 심한 자살 사고를 보고하였다. 심한 자살 사고군에서 심하지 않은 자살 사고군에 비해 BDI, BAI 그리고 사회적 지지체계 척도 점수가 높은 반면, Barthel's index와 NIHSS는 두 군간에 유의한 차이를 보이지 않았다. 심한 자살 사고를 지닌 뇌졸중 환자의 대부분은 우울 증상이나 불안 증상을 가지고 있었으나, 일부의 환자들은 우울 증상이나 불안 증상 없이 자살 사고를 보고하였다. 결론: 급성기 뇌졸중 환자들의 많은 수가 정신과적 평가와 개입이 필요한 자살 사고를 가지고 있었다. 따라서 모든 급성기 뇌졸중 환자들을 대상으로 정기적으로 불안 및 우울 증상에 대한 평가와 더불어 직접 또는 간접적인 방법을 통한 자살 사고의 평가가 반드시 필요할 것으로 생각된다. Objective: The presence of psychiatric disorders including depression and anxiety disorders is considered to be the most important risk factor of suicide. Also, suicidal risk is known to be increased in patients who have serious medical illnesses. Like in patients with other medicalillness, some authors reported that suicidal risk is increased in patients with stroke. But there have been no reports with korean patients. The aim of this study is to investigate the frequency of suicidal ideation of patients in the acute stage of stroke, and to examine the demographic characteristics, and psychiatric and neurological symptoms between the patients with and without suicidal ideation. Method: Seventy seven hospitalized stroke patients at the Hallym Stroke Center from July of 1999 to June of 2000 were included in this study. Beck Suicidal Ideation Scale(BSIS) was used to evaluate suicidal ideation. Beck Depression Inventory(BDI) and Beck Anxiety Inventory(BAI) for depression and aniety, and Social Support Scale(SSS) for social support system of the stroke patients were used. Neurologic disabilities were rated with National Institutes of Health Stroke Scale(NIHSS) and Barthel's Index. Results: Thirty five percent(N=27) of the stroke patients reported severe suicidal ideation. Scores of BDI, BAI and SSS were higher in the patients with severe suicidal ideation than their counterpart. There were no differences in Barthel's index score and NIHSS between two groups. Most patients with severe suicidal ideation had depressive or anxiety symptoms. But patients without depressive or anxiety symptoms also reported severe suicidal ideation. Conclusions: Thirty five percent of stroke patients in acute stage of their illness have severe suicidal ideation that requires careful psychiatric evaluation and intervention. Inclusion of routine assessment of suicidal ideation in these patients is recommended.

Lack of Association between Polymorphisms of the Dopamine Receptor D4 and Dopamine Transporter Genes and Personality Traits in a Korean Population

김세주,김영신,김찬형,이홍식 연세대학교의과대학 2006 Yonsei medical journal Vol.47 No.6

Human personality traits have a considerable genetic component. Cloninger et al. were the first to postulate that certain personality traits, such as novelty seeking, are related to the dopamine neurotransmitter system. In this study, we investigated the associations between dopamine receptor D4 (DRD4) exon III and dopamine transporter (DAT1) polymorphisms and personality traits. The DRD4 and DAT1 gene polymorphisms were genotyped in 214 healthy Korean subjects, whose personality traits were assessed with the Temperament and Character Inventory (TCI). There were no significant differences between scores of TCI temperament dimensions (novelty seeking, harm avoidance, reward dependence, and persistence) and DRD4 gene polymorphism. The DAT1 gene polymorphisms also showed no significant association with any of the temperament subscales of the TCI. These data suggest that DRD4 and DAT1 gene polymorphism may not associated with personality traits in a Korean population.

Identification of Proteolipid Protein 1 Gene Duplication by Multiplex Ligation-Dependent Probe Amplification: First Report of Genetically Confirmed Family of Pelizaeus-Merzbacher Disease in Korea

김세주,윤준식,Hye Jin Baek,서상일,Sook Young Bae,Hyun Jung Cho,기창석 대한의학회 2008 Journal of Korean medical science Vol.23 No.2

Pelizaeus-Merzbacher disease (PMD) is a rare X-linked recessive disorder with a prototype of a dysmyelinating leukodystrophy that is caused by a mutation in the proteolipid protein 1 (PLP1) gene on the long arm of the X chromosome in band Xq22. This mutation results in abnormal expression or production of PLP. We here present a Korean boy with spastic quadriplegia, horizontal nystagmus, saccadic gaze, intentional tremor, head titubation, ataxia, and developmental delay. The brain magnetic resonance imaging (MRI) showed abnormally high signal intensities in the white matter tract, including a subcortical U fiber on the T2-weighted and fluid attenuated inversion recovery (FLAIR) image. The chromosomal analysis was normal; however, duplication of the PLP1 gene in chromosome Xq22 was detected when the multiplex ligation-dependent probe amplification (MLPA) method was used. We also investigated the pedigree for a genetic study related to PMD. This case suggests that the duplication mutation of the PLP1 gene in patients with PMD results in a mild clinical form of the disorder that mimics the spastic quadriplegia of cerebral palsy.

Taxonomic Redescription of Loxophyllum perihoplophorum and L. rostratum (Ciliophora: Pleurostomatida) from Korea

김세주,민기식 한국동물분류학회 2015 Animal Systematics, Evolution and Diversity Vol.31 No.4

Two pleurostomatid ciliates, Loxophyllum perihoplophorum Buddenbrock, 1920 and L. rostratum Cohn, 1866, were collected from the coastal waters of the East Sea, Korea. Their morphologies are described based on live observation and protargol staining, and morphometrics are provided. Loxophyllum perihoplophorum is characterized by the following features: 200-650 μm long in vivo; body slender leaf-shaped, flexible and contractile, with thin and wide extrusome- belted zone; 2 macronuclear nodules (Ma) and 1 micronucleus (Mi); 7-9 contractile vacuoles (CV) positioned along dorsal margin; extrusomes (Ex) evenly distributed along edge of entire body, with about 10 dorsal warts (Wa); 9-11 left (LSK) and 19-22 right somatic kineties (RSK), 4-5 furrows (Fu) on left side. Loxophyllum rostratum is about 100-130 μm long in vivo; body oblate leaf-shaped, contractile, convex ventral side and S-shaped dorsal side, beaklike anterior end; 2 Ma and 1 Mi; 1 CV terminally located; Ex distributed along edge of entire body, with about 9-10 dorsal Wa; 7-8 LSK and 15-19 RSK, ca. 5 Fu on left body side. In addition, sequences of small subunit ribosomal DNA were determined from these two Loxophyllum species and compared with the known Loxophyllum sequences.