Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

Yoon, Hye Eun,Kim, Eun Nim,Kim, Min Young,Lim, Ji Hee,Jang, In-Ae,Ban, Tae Hyun,Shin, Seok Joon,Park, Cheol Whee,Chang, Yoon Sik,Choi, Bum Soon Hindawi Publishing Corporation 2016 Oxidative medicine and cellular longevity Vol.2016 No.-

<P><I>Background</I>. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice.<I> Methods</I>. Histologic changes and expressions of transforming growth factor-<I>β</I> (TGF-<I>β</I>), collagen IV,<I> fibronectin</I>, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG),<I> 3-nitrotyrosine</I>, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice.<I> Results</I>. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-<I>β</I>, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine<SUP>1177</SUP>-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change.<I> Conclusions</I>. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice. </P>

상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

R&D 투자 영향평가 기반 구축 및 시범분석

황석원(Seog-Won, Hwang),오승환(Seung-Hwan, Oh),우청원(Ceung-Won, Woo),장필성(Pil-Seoung, Jang),홍사균(Sa-Gyun, Hong),강희종(Hee-Jong, Kang),최창택(Chang-Taek, Choi),김기환(Gi-Hwan, Kim),이재진(Jae-Jin, Lee),김지훈(Ji-Hoon, Kim) 과학기술정책연구원 2016 정책연구 Vol.- No.-

This study is a late study of “An Exploratory Study on STI policy impact assessment”. In the research of 2015, in principle, it is necessary to evaluate not only R&D investment but also general policies such as various promotion systems, standards and regulations by putting the term “policy impact assessment” in front of it We emphasized the points. However, it is practically very difficultto collect and analyze detailed data of all STI policies. In addition, it is also restricted to allow policy researchers to access data within a certain range. Therefore, this study focuses on the impact assessment of R&D investment, which accounts for the largest percentage of STI policies. In this research, the impact assessment system of R&D investment is analyzed by classifying it into evaluation of economic impact and social impact assessment. Also, the evaluation of economic impact was analyzed separating into a micro viewpoint and a macro viewpoint. First, The analysis of the economic impact assessment of the micro viewpoint relates to how the government’s R&D investment affects enterprises. Specifically, the main analysis target was the employment and investment of companies, the impact on sales and profits. Second, The Analysis for macroeconomic impact assessment on the economy is related to the influence of government’s R&D investment such as national economic growth, employment etc. We also analyzed the spillover effect by industry according to R&D investment. Third, The social impact assessment is to analyze how R&D investment has impacted technology-based social change. This study is a very difficult attempt. However, we analyzed from the point of view of the consumer considering all social change areas as much as possible. The results of the study are summarized as follows. First, the study of economic impact evaluation shows the effect of R&D investment and it can be used as a basis to improve the efficiency and goal of R&D investment in future sectors such as employment, economic growth and investment. Second, through research on social impact assessment, government R&D investment has focused on specific areas in terms of improving the quality of life and social development of people, and confirmed that most social sectors are neglected. In addition, we proposed policy priorities for specific social change indicators and social sectors and proposed long-term R&D investment strategies. Finally, this study is expected to contribute to the establishment of future strategy of private enterprise through the measurement of social change index, the social influence of private research and development, and the influence of technology shock from abroad.

카오틱 신경망을 이용한 비선형 시스템 모사에 관한 연구

장창화,박원우,김상희 金烏工科大學校 1998 論文集 Vol.19 No.-

In this paper, we investigated the identification of a nonlinear dynamic system and chaotic system using chaotic neural networks. Because of chaotic characteristics and self-connections in neuron itself, and interconnections between neural network identifiers could showed good performance in highly nonlinear dynamics such as chaotic system. Simulation results for some nonlinear dynamic system and the chaotic system with chaotic responses were presented to demonstrate robustness of chaotic neural network identifier.

기계중복과 셀간 이동수의 최소화가 가능한 예외적 요소의 제거 방법 : 비용 및 설치대수 제약 고려

장익,윤창원,정병희 한국경영과학회 1998 韓國經營科學會誌 Vol.23 No.4

Using the concept of cellular manufacturing systems(CMS) in job shop manufacturing system is one of the most innovative approaches to improving plant productivity. However. several constraints in machine duplication cost, machining capability. cell space capacity. intercell moves and exceptional elements(EEs) are main problems that prevent achieving the goal of maintaining an ideal CMS environment. Minimizing intercell part traffics and EEs are the main objective of the cell formation problem because it is a critical point that improving production efficiency. Because the intercell moves could be changed according to the sequence of operation, it should be considered in assigning parts and machines to machine cell. This paper presents a method that eliminates EEs under the constraints of machine duplication cost and cell space capacity attaining two goals of minimizing machine duplications and minimizing intercell moves simultaneously. Developing an algorithm that calculates the machine duplications by cell-machine incidence matrix and part-machine incidence matrix. and calculates the exact intercell moves considering the sequence of operation. based on the number of machine duplications and exact intercell moves. the goal programming model which satisfying minimum machine duplications and minimum intercell moves is developed. A linear programming model is suggested that could calculates more effectively without damaging optimal solution. A numerical example is provided to illustrate these methods.

보의 전단 내력에 관한 실험적 연구

장원영,고봉수,은희창,서일교 제주대학교 공과대학 첨단기술연구소 2001 尖端技術硏究所論文集 Vol.12 No.2

The objective of this study is to describe the shear behavior of reinforced concrete beams. Twenty-four specimens were tested with the variables of concrete strength, the strength and spacing of shear bars, and support conditions. The specimens without the shear reinforcement bars showed the shear failure with the abrupt deterioration of shear capacity. The ones with the shear bars exhibited the enhanced capacity with the yielding of longitudinal bars or crushing of compressive concrete. It was observed that the specimens had an flexural effect. Also, the specimens with the continued support condition showed more flexural and shear capacity than the ones with the simple supports, and it was shown that the results satisfied the ACI code.

Langerhans cell histiocytosis

장창덕,허준,박성환,김철훈,오수진,황희성,최설희,신상훈 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Langerhans cell histiocytosis(LCH) appears to arise from Langerhans cell and comprises a spectrum of clinical disease previously described in the literature by a variety of eponyms including histiocytosis X, eosinophilic granuloma, Hand-Christian disease, and Letterer Siwe syndrome. This rare disorder occurs in all groups, predominently affecting children & young adults. LCH has a wide spectrum of clinical features. The differentiation of several forms of this disease is primarily a clinical and not a histologic one. The radiographic characteristics include the appearance of solitary "intraosseous" lesios, the multipicity of "alveolar bone" lesions, the bone lesions, periosteal new bone formation, and slight root resorption. Prognosis of a single bone lesion, is known to be excellent. In contrast, disseminated disease has seen associated with a chronic course, a high rate of morbidity and late consequences, and possible mortality. Treatment of LCH remains problematic. Treatment of multisystem disease, where organ function is being compromised has generally been with high-dose systemic corticosteroids or multiple chemotherapy.

청정지역과 도시지역의 오존농도 특성 연구

장광미,강창희,서명석,허철구,박경윤,이호근,김영준 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

A study has been performed on the characteristics of rural and urban surface ozone concentration for the period of March 1992 to February 1993. The monitoring station of rural ozone is located at Kosan, Cheju and other urban monitoring stations are located at Seoul, Pusan and Kwangju. Rural's and urban's ozone data exhibit a distinct features in many ways. First, annual mean of rural ozone concentration is very high(42 ppbv) but urban's are very low(10~15 ppbv). Second, rural ozone data shows a seasonal variation with it's maximum in spring, and minimum in summer, but urban's show a seasonal variation with it's maximum in spring, and minimum in winter. Third, diurnal variation of rural data is very small but that of urban's are very large. Fourth, monthly mean of daily minimum of rural data is not low and shows a large seasonal variation, but those of urban's data are extremely low(<3 ppbv) and have no seasonal variations.

흰쥐에서 반복적 스트레스가 신경활성 스테로이드의 GABAA 수용체 조절작용에 미치는 영향

장봉석,이종범,박형배,하정희,송창진 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연 구 : 신경활성 스테로이드는 대뇌피질 세포막의 GABAA 수용체 복합체에서 GABAA 수용체 효현제 양상으로 작용하여 [³H]flunitrazepam이 GABAA 수용체에 결합하는 항진 작용이 관찰되었으나, 반복적으로 신경활성 스테로이드를 처치한 세포에서는 이러한 신경활성 스테로이드의 [³H]flunitrazepam 결합 항진효과가 소실되었다는 보고가 있었다. 이에 본 연구에서는 반복적인 스트레스에 부하된 흰쥐를 사용하여 신경활성 스테로이드의 GABAA 수용체에 대한 배위자(ligand)의 결합반응 양상을 정상 실험동물과 비교, 관찰하여 보고자 하였다. 방 법 : 본 연구에서, 벤조다이아제핀 수용체 결합반응(receptor binding assay)을 위한 [3H]flunitrazepam 결합반응을 위해서는 GF/B 여과지(Whatman) 상에서 Brandel M-24R을 사용하여 측정했고, GABAA 수용체 결합반응 (receptor binding assay)을 위한 [3H]muscimol 결합반응에서는 각 시험관 당 100㎕의 대뇌피질 조직(약 0.32mg 단백에 해당)을 사용하였으며, 상기 벤조다이아제핀 수용체 결합반응과 동일한 실험방법을 실시하였다. 결 과 : 신경활성 스테로이드인 pregnanolone은 대뇌피질 세포막의GABAA수용체 복합체에 대한 [³H]flunitrazepam 결합반응을 항진시켜 GABAA 수용체 효현제 양상으로 작용하는 것으로 관찰되었다. 또 pregnanolone이 GABAA 수용체 복합체상의 GABA 결합부위에 대한 결합력을 알아보기 위하여 GABAA 수용체 효현제인 [³H]muscimol 결합반응을 실시하였을 때 pregnanolone은 50%정도의 억제율을 보여주는 GABA와는 그 결과가 다르게 [³H]muscimol 결합반응을 억제하지 않음으로써. muscimol과 경쟁적으로 같은 결합부위에 작용하지 않는다는 것을 보여주었다. 또 반복적인 부동화 스트레스에 부하된 흰쥐의 대뇌 피질조직을 사용하여 pregnanolone의 [³H]flunitrazepam 결합반응을 관찰하였을 때, 대조군에 비하여 pregnanolone의 [³H]flunitrazepam 결합 항진작용이 감약되는 것을 관찰하였다. 결 론 : 이상의 결과로부터 반복적인 스트레스에 부하된 흰쥐의 대뇌피질조직에서는 정상 흰쥐에 비하여, pregnanolone이 GABA성 신경전달에 미치는 항진작용이 감약되었으며, 이는 반복적인 스트레스와 연관된 불안장애의 정신생리 규명에 중요한 정보라고 사료된다. Objectives : Pregnanolone is a potent positive modulator of the γ-aminobutyric acid(GABA) response that enhances the binding of [³H]flunitrazepam to the GABAA receptor. Recently, it was reported that chronic treatment with pregnanolone uncouples allosteric interactions between steroid and benzodiazepine recognition sites. The present study was designed to assess the effect of repeated stress on the modulation of neuroactive steroids on the GABAA receptor. Methods : The effect of steroids on the ligands binding to GABAA receptor was investigated using cerebral cortices of unstressed and repeatedly immobilized rats. Male Sprague-Dawley rats, weighing 200-250g were forced to suffer an immobilization stress for 2 hours. Results : Pregnanolone enhanced the binding of [³H]flunitrazepam to GABAA receptor in both of unstressed and repeatedly stressed rats. However, repeatedly stressed rats showed significantly higher values in EC50 and lower values in Emax of enhancement binding of [³H]flunitrazepam than those of unstressed rats. Conclusions : From these findings, it can be concluded that repeated stress reduced the positive modulation of neuroactive steroid no the GABAA-receptor complex.